Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,283,203	C→T	P21S (CCT→TCT)	kbaY →	tagatose‑1,6‑bisphosphate aldolase 1 subunit KbaY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,283,203	0	C	T	100.0%	243.0 / NA	69	P21S (CCT→TCT)	kbaY	tagatose‑1,6‑bisphosphate aldolase 1 subunit KbaY
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (38/31);  total (38/31)

CCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAATCATTCATCCGTGGCAGGCATAAGAGGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATCCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCC  >  NC_000913/3283072‑3283334                                                                                                                                    |                                                                                                                                    ccAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAATCATTCATCCGTGGCAGGCATAAGAGGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGTCTGCtttt                                                                                                                             >  1:1099998/1‑140 (MQ=255) ccAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAATCATTCATCCGTGGCAGGCATAAGAGGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGTCTGCtttt                                                                                                                             <  2:1099998/140‑1 (MQ=255)    gTGGCACCGTTAACCGCGTGGTGCAAGGCGTAATCATTCATCCGTGGCAGGCATAAGAGGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAAc                                                                                                                          <  1:1100743/140‑1 (MQ=255)       gCACCGTTAACCGCGTGGTGCAAGGCGTAATCATTCATCCGTGGCATGCATAAGAGGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACAtt                                                                                                                       >  2:272769/1‑140 (MQ=255)           cGTTAACCGCGTGGTGCAAGGCGTAATCATTCATCCGTGGCATGCATAAGAGGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATa                                                                                                                   <  1:272769/140‑1 (MQ=255)                   gcgTGGTGCAAGGCGTAATCATTCATCCGTGGCAGGCATAAGAGGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCgag                                                                                                           >  2:1119231/1‑140 (MQ=255)                   gcgTGGTGCAAGGCGTAATCATTCATCCGTGGCAGGCATAAGAGGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCgag                                                                                                           <  1:453247/140‑1 (MQ=255)                     gtggtgCAAGGCGTAATCATTCATCCGTGGCAGGCATAAGAGGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGAc                                                                                                         >  1:1056864/1‑140 (MQ=255)                      tggtgCAAGGCGTAATCATTCATCCGTGGCAGGCATAAGAGGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACa                                                                                                                         >  2:766154/1‑123 (MQ=255)                      tggtgCAAGGCGTAATCATTCATCCGTGGCAGGCATAAGAGGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACa                                                                                                                         <  1:766154/123‑1 (MQ=255)                      tggtgCAAGGCGTAATCATTCATCCGTGGCAGGCATAAGAGGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCga                                                                                                            <  2:896807/136‑1 (MQ=255)                      tggtgCAAGGCGTAATCATTCATCCGTGGCAGGCATAAGAGGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCga                                                                                                            >  1:896807/1‑136 (MQ=255)                       ggtgCAAGGCGTAATCATTCATCCGTGGCAGGCATAAGAGGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGa                                                                                                       >  2:1004328/1‑140 (MQ=255)                         tgCAAGGCGTAATCATTCATCCGTGGCAGGCATAAGAGGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATc                                                                                                     <  2:232867/140‑1 (MQ=255)                              ggCGTAATCATTCATCCGTGGCAGGCATAAGAGGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGc                                                                                                <  2:939817/140‑1 (MQ=255)                              ggCGTAATCATTCATCCGTGGCAGGCATAAGAGGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGc                                                                                                <  1:40109/140‑1 (MQ=255)                                   aaTCATTCATCCGTGGCAGGCATAAGAGGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGAACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATcc                                                                                           >  1:314433/1‑140 (MQ=255)                                    aTCATTCATCCGTGGCAGGCATAAGAGGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCt                                                                                          >  1:108748/1‑140 (MQ=255)                                      cattcatCCGTGGCAGGCATAAGAGGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCg                                                                                        <  1:1116900/140‑1 (MQ=255)                                        ttcatCCGTGGCAGGCATAAGAGGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGaa                                                                                      <  1:1004328/140‑1 (MQ=255)                                          catCCGTGGCAGGCATAAGAGGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAgt                                                                                    <  1:690839/140‑1 (MQ=255)                                             ccGTGGCAGGCATAAGAGGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGtgtg                                                                                 >  2:335136/1‑140 (MQ=255)                                                       caTAAGAGGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATg                                                                       >  1:476812/1‑140 (MQ=255)                                                                aTCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCg                                                              >  1:898060/1‑140 (MQ=255)                                                                 tCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCgg                                                             >  2:1008281/1‑140 (MQ=255)                                                                             cATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATCCTCGCCg                                                 >  1:328513/1‑140 (MQ=255)                                                                               ttATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGAt                                                         >  1:646204/1‑130 (MQ=255)                                                                               ttATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGAt                                                         <  2:646204/130‑1 (MQ=255)                                                                               ttATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATCCTCGCCGGa                                               >  2:563481/1‑140 (MQ=255)                                                                               ttATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATCCTCGCCGGa                                               >  2:154478/1‑140 (MQ=255)                                                                               ttATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATCCTCGCCGGa                                               >  1:322016/1‑140 (MQ=255)                                                                               ttATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATCCTCGCCGGa                                               >  1:367960/1‑140 (MQ=255)                                                                                tATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATCCTCGCCGGaa                                              <  2:367960/140‑1 (MQ=255)                                                                                 aTCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATc                                                        <  1:746438/129‑1 (MQ=255)                                                                                 aTCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATc                                                        >  2:746438/1‑129 (MQ=255)                                                                                     ccACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATCCt                                                      <  1:1041351/127‑1 (MQ=255)                                                                                     ccACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATCCt                                                      >  2:1041351/1‑127 (MQ=255)                                                                                       aCTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATCCTCGCCGGAACGCCggg                                       <  2:1137736/140‑1 (MQ=255)                                                                                         tAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATCCTCGCCGGAACGCCGGGGa                                     <  1:1008281/140‑1 (MQ=255)                                                                                            atatCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATCCTCGCCGGAACGCCGGGGACCt                                  >  2:327298/1‑140 (MQ=255)                                                                                              atCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATCCTCGCCGGAACGCCGGGGACCttt                                >  2:409767/1‑140 (MQ=255)                                                                                                cTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATCCTCGCCGGAACGCCGGGGACCTTTaa                              <  2:108748/140‑1 (MQ=255)                                                                                                     aCAGGACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATCCTCGCCGGAACGCCGGGGACCTTTAAAcaca                         >  1:769983/1‑140 (MQ=255)                                                                                                     aCAGGACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATCCTCGCCGGAACGCCGGGGACCTTTAAAcaca                         >  1:188587/1‑140 (MQ=255)                                                                                                      cAGGTCGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATCCTCGCCGGAACGCCGGGGACCTTTAAACACAt                        <  2:1056864/140‑1 (MQ=255)                                                                                                       aGGACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATATCGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATCCTCGCCGGAACGCCggg                                       <  2:1101798/124‑1 (MQ=255)                                                                                                       aGGACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATCCTCGCCGGAACGCCggg                                       >  1:1101798/1‑124 (MQ=255)                                                                                                        ggACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATCCTCGCCGGAACGCCGGGGACCTTTAAACACAt                        >  2:258912/1‑138 (MQ=255)                                                                                                        ggACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATCCTCGCCGGAACGCCGGGGACCTTTAAACACAt                        <  1:258912/138‑1 (MQ=255)                                                                                                         gACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATCCTCGCCGGAACGCCggg                                       >  2:589685/1‑122 (MQ=255)                                                                                                         gACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATCCTCGCCGGAACGCCggg                                       <  1:589685/122‑1 (MQ=255)                                                                                                         gACGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATCCTCGCCGGAACGCCGGGGACCTTTAAACACATcgc                     <  1:327298/140‑1 (MQ=255)                                                                                                          aCGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATCCTCGCCGGAACGCCGGGGACCTTTAAACACATcgcg                    <  2:769983/140‑1 (MQ=255)                                                                                                           cGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATCCTCGCCGGAACGCCGGGGACCTTTAAACACATCgcgc                   >  2:1103571/1‑140 (MQ=255)                                                                                                           cGCCCAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATCCTCGCCGGAACGCCGGGGACCTTTAAACACATCgcgc                   >  1:94164/1‑140 (MQ=255)                                                                                                             cccAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATCCTCGCCGGAACGCCGGGGACCTTTaa                              >  1:791419/1‑127 (MQ=255)                                                                                                             cccAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATCCTCGCCGGAACGCCGGGGACCTTTaa                              <  2:791419/127‑1 (MQ=255)                                                                                                             cccAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATCCTCGCCGGAACGCCGGGGACCTTTAAACGCATCGCGCTg                 >  1:848433/1‑140 (MQ=255)                                                                                                              ccAGGCCAATGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATCCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTgg                >  2:673256/1‑140 (MQ=255)                                                                                                                      aTGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATCCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATc        >  2:844953/1‑140 (MQ=255)                                                                                                                       tGGCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATCCTCGTCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCt       <  2:758880/140‑1 (MQ=255)                                                                                                                         gCTACGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATCCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTAc     <  2:902840/140‑1 (MQ=255)                                                                                                                            aCGCGGTGTCTGCTTTTAACGTTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATCCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGcc  >  2:861316/1‑140 (MQ=255)                                                                                                                            aCGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATCCTCGCCGGAACGCCGGGGACCTTTAAACACATCgcgc                   >  2:142993/1‑123 (MQ=255)                                                                                                                            aCGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATCCTCGCCGGAACGCCGGGGACCTTTAAACACATCgcgc                   <  1:142993/123‑1 (MQ=255)                                                                                                                            aCGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATCCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAaga            >  1:880868/1‑130 (MQ=255)                                                                                                                            aCGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATCCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAaga            <  2:880868/130‑1 (MQ=255)                                                                                                                            aCGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATCCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGcc  <  1:1103571/140‑1 (MQ=255)                                                                                                                            aCGCGGTGTCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATCCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGcc  >  2:253482/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                    CCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAATCATTCATCCGTGGCAGGCATAAGAGGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATCCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCC  >  NC_000913/3283072‑3283334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑