Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,303,065	T→C	C193R (TGT→CGT)	yhbV →	putative peptidase YhbV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,303,065	0	T	C	100.0%	209.4 / NA	60	C193R (TGT→CGT)	yhbV	putative peptidase YhbV
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/28);  total (32/28)

CTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCTACGTTTACAACCTCGGTAACGAGCTGGCAT  >  NC_000913/3302933‑3303194                                                                                                                                     |                                                                                                                                  cTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGCGTGAAAc                                                                                                                            <  2:893813/140‑1 (MQ=255)    cTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGCGTGAAACctg                                                                                                                         <  1:916003/140‑1 (MQ=255)     ttAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGCGTGAAACctgc                                                                                                                        >  2:975285/1‑140 (MQ=255)      tAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGCGTGAAACctgct                                                                                                                       >  2:15844/1‑140 (MQ=255)           aGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGCGTGAAACCTGCTGCAtt                                                                                                                  <  1:219004/140‑1 (MQ=255)             tGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGACCGAAAGATGAGCGTGAAACCTGCTGCAtt                                                                                                                  <  1:635294/138‑1 (MQ=255)             tGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGACCGAAAGATGAGCGTGAAACCTGCTGCAtt                                                                                                                  >  2:635294/1‑138 (MQ=255)              gCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGCGTGAAACCTGCTGCAATAAg                                                                                                               >  2:1121769/1‑140 (MQ=255)                  tGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGCGTGAAACctg                                                                                                                         <  1:458694/126‑1 (MQ=255)                  tGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGCGTGAAACctg                                                                                                                         >  2:458694/1‑126 (MQ=255)                    aGCTGGGCATTCGTAACCAGTTTGAAGTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCg                                                                                                         >  2:770919/1‑140 (MQ=255)                              tCGTAACCAGTTTGAAGTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCTCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCa                                                                                               >  2:658064/1‑140 (MQ=255)                              tCGTAACCAGTTTGAAGTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCTCGTTCGGAAGACCGCCCGAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCa                                                                                               <  2:1067745/140‑1 (MQ=255)                              tCGTAACCAGTTTGAAGTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCa                                                                                               >  2:1121275/1‑140 (MQ=255)                              tCGTAACCAGTTTGAAGTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCa                                                                                               >  1:733610/1‑140 (MQ=255)                                              gTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGa                                                                               <  2:784766/140‑1 (MQ=255)                                              gTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGa                                                                               <  1:770919/140‑1 (MQ=255)                                               tGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGaa                                                                              <  1:1121275/140‑1 (MQ=255)                                                ggAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGaaa                                                                             <  2:506959/140‑1 (MQ=255)                                                ggAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGaaa                                                                             <  1:130486/140‑1 (MQ=255)                                                          aGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGaaa                                                                             <  1:377119/130‑1 (MQ=255)                                                          aGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGaaa                                                                             >  2:377119/1‑130 (MQ=255)                                                           gCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAgtg                                                                  >  1:62526/1‑140 (MQ=255)                                                              aCGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGttt                                                               >  2:1095502/1‑140 (MQ=255)                                                               cGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACcaaca                                                                      >  2:1021780/1‑132 (MQ=255)                                                               cGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACcaaca                                                                      <  1:1021780/132‑1 (MQ=255)                                                               cGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTg                                                              <  1:279172/140‑1 (MQ=255)                                                                     tCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCa                                                        >  2:514175/1‑140 (MQ=255)                                                                     tCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCa                                                        >  1:106235/1‑140 (MQ=255)                                                                           gCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGCGTGAAACCTGCTGCAATAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCa                                                  <  1:1121769/140‑1 (MQ=255)                                                                            cTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCAt                                                 >  2:511294/1‑140 (MQ=255)                                                                            cTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCAt                                                 >  1:1069986/1‑140 (MQ=255)                                                                             tGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTTCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCAtt                                                <  2:869860/140‑1 (MQ=255)                                                                                ccTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAg                                             >  1:692964/1‑140 (MQ=255)                                                                                    cTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCa                                         >  2:1066988/1‑140 (MQ=255)                                                                                        gcccgcTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAg                                     <  1:1095502/140‑1 (MQ=255)                                                                                         cccgcTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCAtt                                                <  2:764205/128‑1 (MQ=255)                                                                                         cccgcTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCAtt                                                >  1:764205/1‑128 (MQ=255)                                                                                         cccgcTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGc                                    >  1:109174/1‑140 (MQ=255)                                                                                         cccgcTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGc                                    >  1:535466/1‑140 (MQ=255)                                                                                             ctgctTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCt                                <  2:1069986/140‑1 (MQ=255)                                                                                             ctgctTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCt                                <  2:535466/140‑1 (MQ=255)                                                                                             ctgctTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCt                                >  1:613657/1‑140 (MQ=255)                                                                                              tgctTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCTa                               >  1:306142/1‑140 (MQ=255)                                                                                                     ccGCGCGTTCGGAAGACCGCTCGAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCTACGTTTAc                        <  2:306142/140‑1 (MQ=255)                                                                                                      cgcgcgTTCGGAAGACCGCCCGAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCTACGTTTACa                       <  2:109174/140‑1 (MQ=255)                                                                                                        cgcgTTCGGAAGACCGCCCGAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCTACGTTTAc                        >  1:1035611/1‑137 (MQ=255)                                                                                                        cgcgTTCGGAAGACCGCCCGAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCTACGTTTAc                        <  2:1035611/137‑1 (MQ=255)                                                                                                          cgTTCGGAAGACCGCCCGAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCTACGTTTACAACCt                   >  2:541353/1‑140 (MQ=255)                                                                                                           gTTCGGAAGACCGCCCGAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCTACGTTTACaa                      <  2:541596/136‑1 (MQ=255)                                                                                                           gTTCGGAAGACCGCCCGAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCTACGTTTACaa                      >  1:541596/1‑136 (MQ=255)                                                                                                                       gCCCGAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCTACGTTTACAACCTCGGTAACGAGCTg      >  1:948025/1‑140 (MQ=255)                                                                                                                       gCCCGAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCTACGTTTACAACCTCGGTAACGAGCTg      >  1:73674/1‑140 (MQ=255)                                                                                                                        cccGAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCTACGTTTACAACCTCGGTAACGAGCTgg     >  2:1034919/1‑140 (MQ=255)                                                                                                                        cccGAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCTACGTTTACAACCTCGGTAACGAGCTgg     >  2:937489/1‑140 (MQ=255)                                                                                                                         ccGAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCTACGTTTACAACCTCGGTAAc            >  1:999108/1‑132 (MQ=255)                                                                                                                         ccGAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCTACGTTTACAACCTCGGTAAc            <  2:999108/132‑1 (MQ=255)                                                                                                                          cGAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCTACGTTTACAACCTCGGTAACGAGCTGGCa   <  1:975285/140‑1 (MQ=255)                                                                                                                           gAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCTACGTTTACAACCTCGGTAACGAGCTGGCAt  <  1:658064/140‑1 (MQ=255)                                                                                                                           gAAAGATGAGCGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCTACGTTTACAACCTCGGTAACGAGCTGGCAt  <  1:15844/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                  CTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACCTGCTGCATTAAGTATCCGAACGGGCGCAACGTGCTGTCGCAGGAAAACCAACAAGTGTTTGTACTCAATGGCATTCAGACCATGAGCGGCTACGTTTACAACCTCGGTAACGAGCTGGCAT  >  NC_000913/3302933‑3303194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑