Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,303,947	T→C	V167A (GTG→GCG)	yhbW →	putative luciferase‑like monooxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,303,947	0	T	C	100.0%	152.6 / NA	45	V167A (GTG→GCG)	yhbW	putative luciferase‑like monooxygenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/22);  total (23/22)

TGGCGCTACGTCGTCATATGAGCGGCGATATTGATAATTTCCCCCGCGATGTGGCGGAGCTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGTGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCCGTTTGCGTTTGCCTCACACTTCGCGCCGGATATGCTGTTCCAGGCGCTGCATCTTTATCGCAGCAACTTCAAACC  >  NC_000913/3303812‑3304079                                                                                                                                        |                                                                                                                                     tgGCGCTACGTCGTCATATGAGCGGCGATATTGATAATTTCCCCCGCGATGTGGCGGAGCTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTgg                                                                                                                                  <  1:1143442/140‑1 (MQ=255)        aCGTCGTCATATGAGCGGCGATATTGATAATTTCCCCCGCGATGTGGCGGAGCTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCgcgt                                                                                                                                    >  1:832526/1‑131 (MQ=255)         cgtcgtCATATGAGCGGCGATATTGATAATTTCCCCCGCGATGTGGCGGAGCTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAgg                                                                                                                          >  1:995462/1‑140 (MQ=255)         cgtcgtCATATGAGCGGCGATATTGATAATTTCCCCCGCGATGTGGCGGAGCTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAgg                                                                                                                          >  1:546291/1‑140 (MQ=255)           tcgtcATATGAGCGGCGATATTGATAATTTCCCCCGCGATGTGGCGGAGCTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCt                                                                                                                        <  2:488357/140‑1 (MQ=255)           tcgtcATATGAGCGGCGATATTGATAATTTCCCCCGCGATGTGGCGGAGCTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCt                                                                                                                        <  1:795608/140‑1 (MQ=255)                       cggcgATATTGATAATTTCCCCCGCGATGTGGCGGAGCTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACa                                                                                                            >  1:64825/1‑140 (MQ=255)                         gcgATATTGATAATTTCCCCCGCGATGTGGCGGAGCTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAgc                                                                                                          >  1:1144248/1‑140 (MQ=255)                         gcgATATTGATAATTTCCCCCGCGATGTGGCGGAGCTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAgc                                                                                                          >  2:925777/1‑140 (MQ=255)                          cgATATTGATAATTTCCCCCGCGATGTGGCGGAGCTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCt                                                                                                                        <  1:1065774/125‑1 (MQ=255)                          cgATATTGATAATTTCCCCCGCGATGTGGCGGAGCTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCt                                                                                                                        >  2:1065774/1‑125 (MQ=255)                            atatTGATAATTTCCCCCGCGATGTGGCGGAGCTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAgcgcg                                                                                                       >  1:970851/1‑140 (MQ=255)                                  aTAATTTCCCCCGCGATGTGGCGGAGCTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTg                                                                                                 <  2:1144248/140‑1 (MQ=255)                                           cccGCGATGTGGCGGAGCTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAg                                                                                        >  2:622984/1‑140 (MQ=255)                                            ccGCGATGTGGCGGAGCTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGc                                                                                       <  2:546291/140‑1 (MQ=255)                                                gATGTGGCGGAGCTGGTGGACTGGTTTGACGCCCGCGATCCC‑‑‑CCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTggcg                                                                                              <  1:69109/126‑1 (MQ=255)                                                gATGTGGCGGAGCTGGTGGACTGGTTTGACGCCCGCGATCCC‑‑‑CCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTggcg                                                                                              >  2:69109/1‑126 (MQ=255)                                                         gAGCTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTggcggc                                                                                            <  1:1131369/122‑1 (MQ=255)                                                         gAGCTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTggcggc                                                                                            >  2:1131369/1‑122 (MQ=255)                                                         gAGCTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCcgtt                                                                          <  1:622984/140‑1 (MQ=255)                                                          aGCTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCcgttt                                                                         >  2:897575/1‑140 (MQ=255)                                                            cTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGGCCGGTACCAGGCTATGGCGCGAAAAACCCCGCGTGGTTGTTAGGCCCCAGCCCTTTCAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCcgttttc                                                                       >  2:322363/1‑138 (MQ=255)                                                            cTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGc                                                                                       <  1:763012/124‑1 (MQ=255)                                                            cTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGc                                                                                       >  2:763012/1‑124 (MQ=255)                                                                tggACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCcgtttgcgttt                                                                   >  1:851185/1‑140 (MQ=255)                                                                      ggTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCCGTTTGCGTTTGCCTca                                                             <  1:897575/140‑1 (MQ=255)                                                                      ggTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCCGTTTGCGTTTGCCTca                                                             >  1:351067/1‑140 (MQ=255)                                                                      ggTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCCGTTTGCGTTTGCCTca                                                             <  2:970851/140‑1 (MQ=255)                                                                         ttGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCcgtttgcgttt                                                                   >  1:896489/1‑131 (MQ=255)                                                                         ttGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCcgtttgcgttt                                                                   <  2:896489/131‑1 (MQ=255)                                                                         ttGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCCGTTTGCGTTTGCCTCacac                                                          >  2:727681/1‑140 (MQ=255)                                                                                ccGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCCGTTTGCGTTTGCCTCACACTTCgcgc                                                   <  2:64825/140‑1 (MQ=255)                                                                                          aaTCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCCGTTTGCGTTTGCCTCACACTTCGCGCCGGa                                               >  2:807833/1‑134 (MQ=255)                                                                                          aaTCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCCGTTTGCGTTTGCCTCACACTTCGCGCCGGa                                               <  1:807833/134‑1 (MQ=255)                                                                                                cATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCCGTTTGCGTTTGCCTCACACTTCGCGCCGGATATGCTGTTCCa                                   >  2:857446/1‑140 (MQ=255)                                                                                                cATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCCGTTTGCGTTTGCCTCACACTTCGCGCCGGATATGCTGTTCCa                                   <  2:995462/140‑1 (MQ=255)                                                                                                cATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCCGTTTGCGTTTGCCTCACACTTCGCGCCGGATATGCTGTTCCa                                   >  2:105609/1‑140 (MQ=255)                                                                                                  tgtgCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCCGTTTGCGTTTGCCTCACACTTCGCGCCGGATATGCTGTTCCAgg                                 <  1:274010/140‑1 (MQ=255)                                                                                                   gtgCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCCGTTTGCGTTTGCCTCACACTTCGCGCCGGATATGCTGTTCCAGgc                                <  2:851185/140‑1 (MQ=255)                                                                                                    tgCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCCGTTTGCGTTTGCCTCACACTTCGCGCCGGATATGCTGTTCCAGgcg                               <  1:322363/140‑1 (MQ=255)                                                                                                       gcCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCCGTTTGCGTTTGCCTCACACTTCGCGCCGGATATGCTGTTCCAGGCGCTg                            <  1:704942/140‑1 (MQ=255)                                                                                                        cccGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCCGTTTGCGTTTGCCTCACACTTCGCGCCGGATATGCTGTTCCAGGCGCTGc                           <  1:105609/140‑1 (MQ=255)                                                                                                                   gCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCCGTTTGCGTTTGCCTCACACTTCGCGCCGGATATGCTGTTCCAGGCGCTGCATCTTTATCgc                >  2:708885/1‑140 (MQ=255)                                                                                                                       tGGCGAGAAAATCCCCGCGTGGTTGTTAGGTTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCCGTTTGCGTTTGCCTCACACTTCGCGCCGGATATGCTGTTCCAGGCGCTGCATCTTTATCgcagca            >  2:49799/1‑140 (MQ=255)                                                                                                                        ggCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCCGTTTGCGTTTGCCTCACACTTCGCGCCGGATATGCTGTTCCAGGCGCTGCATCTTTATCGCAGCaa           >  2:731923/1‑140 (MQ=255)                                                                                                                                   ccccGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCCGTTTGCGTTTGCCTCACACTTCGCGCCGGATATGCTGTTCCAGGCGCTGCATCTTTATCGCAGCAACTTCAAAcc  <  2:328321/138‑1 (MQ=255)                                                                                                                                   ccccGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCCGTTTGCGTTTGCCTCACACTTCGCGCCGGATATGCTGTTCCAGGCGCTGCATCTTTATCGCAGCAACTTCAAAcc  >  1:328321/1‑138 (MQ=255)                                                                                                                                        |                                                                                                                                     TGGCGCTACGTCGTCATATGAGCGGCGATATTGATAATTTCCCCCGCGATGTGGCGGAGCTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGTGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCCGTTTGCGTTTGCCTCACACTTCGCGCCGGATATGCTGTTCCAGGCGCTGCATCTTTATCGCAGCAACTTCAAACC  >  NC_000913/3303812‑3304079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑