Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,368,837	T→C	G337G (GGT→GGC)	yhcG →	DUF1016 domain‑containing protein YhcG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,368,837	0	T	C	100.0%	196.9 / NA	56	G337G (GGT→GGC)	yhcG	DUF1016 domain‑containing protein YhcG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/24);  total (32/24)

GCGATGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTC  >  NC_000913/3368704‑3368973                                                                                                                                      |                                                                                                                                         gCGATGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGCTTGCCt                                                                                                                                    >  1:594947/1‑140 (MQ=255)  cGATGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGCTTGCCTa                                                                                                                                   >  2:72905/1‑140 (MQ=255)     tGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGCTTGCCTAaca                                                                                                                                <  2:100297/140‑1 (MQ=255)      gCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGCTTGCCTAacac                                                                                                                               >  2:541899/1‑140 (MQ=255)          gACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGCTTGCCTAACACCGtt                                                                                                                           <  1:934250/140‑1 (MQ=255)          gACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGCTTGCCTAACACCGtt                                                                                                                           >  1:295167/1‑140 (MQ=255)              gATGAATATGTATCTCAACTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGCTTGCCTAACACCGTTCTgg                                                                                                                       <  1:290082/140‑1 (MQ=255)                   atatGTATCTCAACTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGCTTGCCTAACACCGTTCTGGCAAGc                                                                                                                  <  1:317234/140‑1 (MQ=255)                         aTCTCAACTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGCTTGCCTAACACCGTTCTGGCAAGCGAatat                                                                                                            >  2:1072499/1‑140 (MQ=255)                         aTCTCAACTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGCTTGCCTAACACCGTTCTGGCAAGCGAatat                                                                                                            >  2:574579/1‑140 (MQ=255)                              aaCTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGCTTGCCTAACACCGTTCTGGCAAGCGAATATAAGAt                                                                                                       >  1:324792/1‑140 (MQ=255)                              aaCTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGCTTGCCTAACACCGTTCTGGCAAGCGAATATAAGAt                                                                                                       >  1:738509/1‑140 (MQ=255)                              aaCTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGCTTGCCTAACACCGTTCTGGCAAGCGAATATAAGAt                                                                                                       >  1:1086839/1‑140 (MQ=255)                               aCTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGCTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATg                                                                                                      >  2:1043516/1‑140 (MQ=255)                               aCTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGCTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATg                                                                                                      >  1:1027332/1‑140 (MQ=255)                                cTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGCTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGc                                                                                                     >  2:781312/1‑140 (MQ=255)                                 tACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGCTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCa                                                                                                    <  2:324792/140‑1 (MQ=255)                                   cGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGCTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAAc                                                                                                  <  1:1043516/140‑1 (MQ=255)                                   cGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGCTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAAc                                                                                                  >  2:797122/1‑140 (MQ=255)                                    gCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGCTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACt                                                                                                 >  2:187377/1‑140 (MQ=255)                                      cAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGCTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTAc                                                                                               <  1:936925/140‑1 (MQ=255)                                      cAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGCTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTAc                                                                                               <  1:574579/140‑1 (MQ=255)                                           agCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGCTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCtgat                                                                                          <  2:295167/140‑1 (MQ=255)                                              aCTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGCTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATgag                                                                                       >  1:338252/1‑140 (MQ=255)                                                 ggACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGCTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGaaa                                                                                    <  2:738509/140‑1 (MQ=255)                                                 ggACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGCTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGaaa                                                                                    <  2:1086839/140‑1 (MQ=255)                                                       tACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGCTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTc                                                                              >  2:738175/1‑140 (MQ=255)                                                            gATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGCTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGa                                                                         >  2:586643/1‑140 (MQ=255)                                                                   aTCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGCTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTc                                                                  >  2:249555/1‑140 (MQ=255)                                                                                tCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGCTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAgg                                                     <  2:1027332/140‑1 (MQ=255)                                                                                  tGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGCTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCg                                                   <  1:1072499/140‑1 (MQ=255)                                                                                            tgCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGCTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAgg                                         >  1:37872/1‑140 (MQ=255)                                                                                            tgCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGCTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAgg                                         <  2:683040/140‑1 (MQ=255)                                                                                             gCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGCTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGa                                        <  2:338252/140‑1 (MQ=255)                                                                                              cAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGCTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAgg                                         <  1:380303/138‑1 (MQ=255)                                                                                              cAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGCTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAgg                                         >  2:380303/1‑138 (MQ=255)                                                                                                      ggAGCCGGAGAAGCGCATTATGCTCTGGCAGGCTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAg                               >  1:514329/1‑140 (MQ=255)                                                                                                       gAGCCGGAGAAGCGCATTATGCTCTGGCAGGCTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGa                              <  2:1021390/140‑1 (MQ=255)                                                                                                          ccGGAGAAGCGCATTATGCTCTGGCAGGCTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGc                           >  2:728718/1‑140 (MQ=255)                                                                                                           cGGAGAAGCGCATTATGCTCTGGCAGGCTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGcc                          >  2:98419/1‑140 (MQ=255)                                                                                                             gagaAGCGCATTATGCTCTGGCAGGCTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGt                        >  2:987962/1‑140 (MQ=255)                                                                                                              agaAGCGCATTATGCTCTGGCAGGCTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCta                                  <  2:69122/129‑1 (MQ=255)                                                                                                              agaAGCGCATTATGCTCTGGCAGGCTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCta                                  >  1:69122/1‑129 (MQ=255)                                                                                                                     cATTATGCTCTGGCAGGCTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCa                >  2:419539/1‑140 (MQ=255)                                                                                                                        tATGCTCTGGCAGGCTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCg             >  1:732188/1‑140 (MQ=255)                                                                                                                          tGCTCTGGCAGGCTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGt           >  2:343617/1‑140 (MQ=255)                                                                                                                           gCTCTGGCAGGCTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGtt          <  1:249555/140‑1 (MQ=255)                                                                                                                             tctGGCAGGCTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTc        <  1:738175/140‑1 (MQ=255)                                                                                                                               tGGCAGGCTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGa      <  2:732188/140‑1 (MQ=255)                                                                                                                                ggCAGGCTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGaa     >  2:654763/1‑140 (MQ=255)                                                                                                                                  cAGGCTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAAtt   >  2:58937/1‑140 (MQ=255)                                                                                                                                  cAGGCTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAAtt   >  1:1104492/1‑140 (MQ=255)                                                                                                                                  cAGGCTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAAt    >  2:517527/1‑139 (MQ=255)                                                                                                                                  cAGGCTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAAt    <  1:517527/139‑1 (MQ=255)                                                                                                                                   aGGCTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTc  <  1:987962/140‑1 (MQ=255)                                                                                                                                   aGGCTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTc  <  1:98419/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         GCGATGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTC  >  NC_000913/3368704‑3368973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑