| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,555,953 | T→C | E299E (GAA→GAG) | rtcA ← | RNA 3'‑terminal phosphate cyclase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,555,953 | 0 | T | C | 98.3% | 186.4 / ‑4.3 | 59 | E299E (GAA→GAG) | rtcA | RNA 3'‑terminal phosphate cyclase |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (30/28); total (31/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.32e-01 | |||||||||||
ACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCG > NC_000913/3555822‑3556084 | aCATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCcgc < 2:247614/140‑1 (MQ=255) cATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCcgcg < 1:822708/140‑1 (MQ=255) ctGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGcgcccgc > 1:901037/1‑140 (MQ=255) atcatTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAgc < 1:1011817/140‑1 (MQ=255) ttCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTccc > 1:988148/1‑122 (MQ=255) ttCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTccc < 2:988148/122‑1 (MQ=255) ttCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCa > 2:768639/1‑140 (MQ=255) ttCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCa > 1:590517/1‑140 (MQ=255) tCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGACATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCAt > 1:866047/1‑140 (MQ=255) aaTGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCg > 1:1150740/1‑140 (MQ=255) tcccccGCGTTACGCCATCTGTTTCTACCAAACTAAACCGCACCGGCAAGACACGCTCCCCCACCTCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGc < 2:577497/137‑1 (MQ=255) tCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGc > 2:902096/1‑140 (MQ=255) cACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCa > 1:280794/1‑140 (MQ=255) aCCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGcgcccgc < 2:687414/122‑1 (MQ=255) aCCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGcgcccgc > 1:687414/1‑122 (MQ=255) aCCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCAc > 1:880017/1‑140 (MQ=255) aCCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCAc > 2:469427/1‑140 (MQ=255) cgTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCg < 2:928370/128‑1 (MQ=255) cgTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGtt > 2:441005/1‑140 (MQ=255) cgTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCACCAGCGCCATCg > 1:928370/1‑128 (MQ=255) gTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTg < 1:238316/140‑1 (MQ=255) tACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGt < 1:441005/140‑1 (MQ=255) tACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGt < 2:901037/140‑1 (MQ=255) cATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCa < 2:280794/140‑1 (MQ=255) cTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGa < 1:311070/140‑1 (MQ=255) tGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGat < 2:107332/140‑1 (MQ=255) gTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGata > 1:981523/1‑140 (MQ=255) ttCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGata > 1:762050/1‑138 (MQ=255) ttCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGata < 2:762050/138‑1 (MQ=255) cTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTcc < 1:902096/140‑1 (MQ=255) tATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTccc > 1:290699/1‑140 (MQ=255) tCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTccccc > 2:233475/1‑140 (MQ=255) aaaCCGCCCCGTCAAGAAACGCCCCACCTCCGCGATTTTGGTCAGCAGATGGCAGGGGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCAccgccg < 2:829854/140‑1 (MQ=255) cACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTTCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCAccgccg > 1:388369/1‑134 (MQ=255) cACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCAccgccg < 2:388369/134‑1 (MQ=255) aCCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCt > 1:658542/1‑140 (MQ=255) gCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGcc > 1:160607/1‑140 (MQ=255) aCGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGAGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGc < 1:907113/140‑1 (MQ=255) tCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGttt > 1:1101357/1‑140 (MQ=255) tCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGttt > 2:466140/1‑140 (MQ=255) ccaccaCCGCAATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTc < 2:563699/140‑1 (MQ=255) accaccGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCAc < 2:154842/140‑1 (MQ=255) caccGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACct < 2:229962/140‑1 (MQ=255) caccGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACct > 1:229962/1‑140 (MQ=255) cgcgATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCtt > 1:1130995/1‑140 (MQ=255) cgATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTc > 1:292768/1‑140 (MQ=255) tatTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCAcc < 1:768639/140‑1 (MQ=255) ggTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACt < 1:1058627/140‑1 (MQ=255) gTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACtg > 2:822512/1‑140 (MQ=255) agcagATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGc < 2:880017/140‑1 (MQ=255) aTGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCaa > 2:1002202/1‑140 (MQ=255) aTGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCaa > 2:934290/1‑140 (MQ=255) tGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAac < 1:934290/140‑1 (MQ=255) tGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAac < 1:469427/140‑1 (MQ=255) tGGCATGAGGGATGGGCGACCGTAAACCCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAac < 2:867932/140‑1 (MQ=255) cATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAaccac < 1:466140/140‑1 (MQ=255) tGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACt > 2:454471/1‑140 (MQ=255) gggCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTg > 1:577862/1‑140 (MQ=255) gCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGAc < 1:454471/140‑1 (MQ=255) gCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGAc < 2:1150740/140‑1 (MQ=255) cGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGAcg < 1:822512/140‑1 (MQ=255) aCCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGAcgcg < 2:981523/140‑1 (MQ=255) aCCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCA‑CAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGAcg > 2:252452/1‑137 (MQ=255) aCCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCA‑CAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGAcg < 1:252452/137‑1 (MQ=255) | ACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCG > NC_000913/3555822‑3556084 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |