Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,566,281	C→T	A101T (GCA→ACA)	glgP ←	glycogen phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,566,281	0	C	T	100.0%	126.1 / NA	36	A101T (GCA→ACA)	glgP	glycogen phosphorylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/19);  total (17/19)

CCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTAAACCTGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAG  >  NC_000913/3566145‑3566414                                                                                                                                         |                                                                                                                                      ccGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGTGcc                                                                                                                                    <  1:539922/140‑1 (MQ=255)    cgcCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGTGcc                                                                                                                                    >  1:1108333/1‑137 (MQ=255)    cgcCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGTGcc                                                                                                                                    <  2:1108333/137‑1 (MQ=255)    cgcCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGTGCCCTg                                                                                                                                 <  1:1103771/140‑1 (MQ=255)              aCCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGTGCCCTGTACATCTTCg                                                                                                                       <  1:663757/140‑1 (MQ=255)                          ccAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGc                                                                                                           >  1:345101/1‑140 (MQ=255)                            agagAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGa                                                                                                         <  1:560698/140‑1 (MQ=255)                                  tCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACAt                                                                                                   >  2:309339/1‑140 (MQ=255)                                   cAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATg                                                                                                  <  2:906377/140‑1 (MQ=255)                                          gcaagcCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTgg                                                                                           <  2:382028/140‑1 (MQ=255)                                                  ccAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTa                                                                                   >  2:451176/1‑140 (MQ=255)                                                         tCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCaa                                                                            >  1:400190/1‑140 (MQ=255)                                                                    cGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTcc                                                                 >  1:116335/1‑140 (MQ=255)                                                                        aCCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCg                                                             >  2:30205/1‑140 (MQ=255)                                                                           gAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACa                                                          <  2:838709/140‑1 (MQ=255)                                                                               ccTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGgta                                                      >  1:1013669/1‑140 (MQ=255)                                                                                  gggTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGgtagta                                                   <  1:451176/140‑1 (MQ=255)                                                                                   ggTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTaa                                                  >  1:120973/1‑140 (MQ=255)                                                                                              tcatcaATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTAAACCTGACGAGt                                       >  1:871081/1‑140 (MQ=255)                                                                                               catcaATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTAAACCTGACGAGtt                                      >  2:800300/1‑140 (MQ=255)                                                                                                     tcaGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTAAACCTGACGAGTTTCTTGc                                <  2:120973/140‑1 (MQ=255)                                                                                                        gCTCTTCGAGATTTAACCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTAAACCTGACGAGTTTCTTGCGAc                             <  1:590028/140‑1 (MQ=255)                                                                                                               gagaTTTAACCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTAAACCTGACGAGTTTCTTGCGACAACTggg                      <  1:63638/140‑1 (MQ=255)                                                                                                               gagaTTTAACCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTAAACCTGACGAGTTTCTTGCGACAACTggg                      >  1:681044/1‑140 (MQ=255)                                                                                                                 gaTTTAACCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTAAACCTGACGAGTTTCTTGCGACAACTGGGCa                    <  2:400190/140‑1 (MQ=255)                                                                                                                  aTTTAACCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTAAACCTGACGAGTTTCTTGCGACAACTGGGCAc                   <  2:116335/140‑1 (MQ=255)                                                                                                                     tAACCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTAAACCTGACGAGTTTCTTGCGACAACTGGGCACGGt                <  1:800300/140‑1 (MQ=255)                                                                                                                      aaCCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTAAACCTGACGAGTTTCTTGCGACAACTGGGCACGGtt               <  2:681044/140‑1 (MQ=255)                                                                                                                      aaCCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTAAACCTGACGAGTTTCTTGCGACAACTGGGCACGGtt               >  2:244838/1‑140 (MQ=255)                                                                                                                      aaCCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTAAACCTGACGAGTTTCTTGCGACAACTGGGCACGGtt               <  1:1075625/140‑1 (MQ=255)                                                                                                                            aTCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTAAACCTGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACg         >  1:1038760/1‑140 (MQ=255)                                                                                                                             tCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTAAACCTGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGt        >  2:146051/1‑140 (MQ=255)                                                                                                                             tCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTAAACCTGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGt        >  1:992564/1‑140 (MQ=255)                                                                                                                             tCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTAAACCTGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGt        >  2:923848/1‑140 (MQ=255)                                                                                                                                   ccAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTAAACCTGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAg  <  1:244838/140‑1 (MQ=255)                                                                                                                                   ccAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTAAACCTGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAg  <  1:146051/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      CCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTAAACCTGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAG  >  NC_000913/3566145‑3566414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑