Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,580,766	T→C	T14A (ACC→GCC)	yhhX ←	putative oxidoreductase YhhX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,580,766	0	T	C	100.0%	240.9 / NA	69	T14A (ACC→GCC)	yhhX	putative oxidoreductase YhhX
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/32);  total (37/32)

CGTCGAGATCGCTGGTGAAATGGATATGGGAATAAATGGGAGCCTGTTCTTCCGGCTTCGCATGGCGACGAAAAATATGCGCGACATGCCAGCTATCCTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGTGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGTCCTTTTTAAGGTGGTTATTTACACCTTAGCGCAAAGCGGACGTGGTTCCTACTGGAGTGCGCATAACGAACACAAGCACTCCCGT  >  NC_000913/3580631‑3580898                                                                                                                                        |                                                                                                                                     cgtcgAGATCGCTGGTGAAATGGATATGGGAATAAATGGGAGCCTGTTCTTCCGGCTTCGCATGGCGACGAAAAATATGCGCGACATGCCAGCTATCCTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCtt                                                                                                                                  >  2:815843/1‑140 (MQ=255) cgtcgAGATCGCTGGTGAAATGGATATGGGAATAAATGGGAGCCTGTTCTTCCGGCTTCGCATGGCGACGAAAAATATGCGCGACATGCCAGCTATCCTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCtt                                                                                                                                  >  2:735225/1‑140 (MQ=255)      agaTCGCTGGTGAAATGGATATGGGAATAAATGGGAGCCTGTTCTTCCGGCTTCGCATGGCGACGAAAAATATGCGCGACATGCCAGCTATCCTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCTTTTGcc                                                                                                                             >  1:366106/1‑140 (MQ=255)       gaTCGCTGGTGAAATGGATATGGGAATAAATGGGAGCCTGTTCTTCCGGCTTCGCATGGCGACGAAAAATATGCGCGACATGCCAGCTATCCTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCg                                                                                                                            <  1:735225/140‑1 (MQ=255)       gaTCGCTGGTGAAATGGATATGGGAATAAATGGGAGCCTGTTCTTCCGGCTTCGCATGGCGACGAAAAATATGCGCGACATGCCAGCTATCCTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCg                                                                                                                            <  1:1113507/140‑1 (MQ=255)       gaTCGCTGGTGAAATGGATATGGGAATAAATGGGAGCCTGTTCTTCCGGCTTCGCATGGCGACGAAAAATATGCGCGACATGCCAGCTATCCTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCg                                                                                                                            <  1:206535/140‑1 (MQ=255)         tCGCTGGTGAAATGGATATGGGAATAAATGGGAGCCTGTTCTTCCGGCTTCGCATGGCGACGAAAAATATGCGCGACATGCCAGCTATCCTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCGaa                                                                                                                          <  2:157537/140‑1 (MQ=255)                  aaaTGGATATGGGAATAAATGGGAGCCTGTTCTTCCGGCTTCGCATGGCGACGAAAAATATGCGCGACATGCCAGCTATCCTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATaaa                                                                                                                 >  1:680738/1‑140 (MQ=255)                  aaaTGGATATGGGAATAAATGGGAGCCTGTTCTTCCGGCTTCGCATGGCGACGAAAAATATGCGCGACATGCCAGCTATCCTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATaaa                                                                                                                 >  2:810111/1‑140 (MQ=255)                    aTGGATATGGGAATAAATGGGAGCCTGTTCTTCCGGCTTCGCATGGCGACGAAAAATATGCGCGACATGCCAGCTATCCTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCg                                                                                                                            >  2:463224/1‑127 (MQ=255)                    aTGGATATGGGAATAAATGGGAGCCTGTTCTTCCGGCTTCGCATGGCGACGAAAAATATGCGCGACATGCCAGCTATCCTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCg                                                                                                                            <  1:463224/127‑1 (MQ=255)                         tatGGGAATAAATGGGAGCCTGTTCTTCCGGCTTCGCATGGCGACGAAAAATATGCGCGACATGCCAGCTATCCTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCGaa                                                                                                                          <  2:518134/124‑1 (MQ=255)                         tatGGGAATAAATGGGAGCCTGTTCTTCCGGCTTCGCATGGCGACGAAAAATATGCGCGACATGCCAGCTATCCTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCGaa                                                                                                                          >  1:518134/1‑124 (MQ=255)                         tatGGGAATAAATGGGAGCCTGTTCTTCCGGCTTCGCATGGCGACGAAAAATATGCGCGACATGCCAGCTATCCTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAg                                                                                                          >  1:241587/1‑140 (MQ=255)                         tatGGGAATAAATGGGAGCCTGTTCTTCCGGCTTCGCATGGCGACGAAAAATATGCGCGACATGCCAGCTATCCTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAg                                                                                                          >  2:566182/1‑140 (MQ=255)                            gggAATAAATGGGAGCCTGTTCTTCCGGCTTCGCATGGCGACGAAAAATATGCGCGACATGCCAGCTATCCTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTtg                                                                                                       >  1:1078700/1‑140 (MQ=255)                               aaTAAATGGGAGCCTGTTCTTCCGGCTTCGCATGGCGACGAAAAATATGCGCGACATGCCAGCTATCCTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTtgatg                                                                                                    >  2:319011/1‑140 (MQ=255)                                  aaaTGGGAGCCTGTTCTTCCGGCTTCGCATGGCGACGAAAAATATGCGCGACATGCCAGCTATCCTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGAcc                                                                                                 >  2:1012141/1‑140 (MQ=255)                                        gAGCCTGTTCTTCCGGCTTCGCATGGCGACGAAAAATATGCGCGACATGCCAGCTATCCTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGAtt                                                                                           >  2:530160/1‑140 (MQ=255)                                         aGCCTGTTCTTCCGGCTTCGCATGGCGACGAAAAATATGCGCGACATGCCAGCTATCCTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGAttt                                                                                          >  2:816180/1‑140 (MQ=255)                                          gCCTGTTCTTCCGGCTTCGCATGGCGACGAAAAATATGCGCGACATGCCAGCTATCCTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTc                                                                                         >  2:882808/1‑140 (MQ=255)                                           ccTGTTCTTCCGGCTTCGCATGGCGACGAAAAATATGCGCGACATGCCAGCTATCCTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCg                                                                                        >  2:738180/1‑140 (MQ=255)                                            cTGTTCTTCCGGCTTCGCATGGCGACGAAAAATATGCGCGACATGCCAGCTATCCTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGt                                                                                       <  1:816180/140‑1 (MQ=255)                                             tGTTCTTCCGGCTTCGCATGGCGACGAAAAATATGCGCGACATGCCAGCTATCCTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGTc                                                                                      >  1:1056175/1‑140 (MQ=255)                                                tcttcCGGCTTCGCATGGCGACGAAAAATATGCGCGACATGCCAGCTATCCTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGt                                                                                       <  1:1099506/136‑1 (MQ=255)                                                tcttcCGGCTTCGCATGGCGACGAAAAATATGCGCGACATGCCAGCTATCCTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGt                                                                                       >  2:1099506/1‑136 (MQ=255)                                                    ccGGCTTCGCATGGCGACGAAAAATATGCGCGACATGCCAGCTATCCTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGTCCTTTTTa                                                                               <  2:366106/140‑1 (MQ=255)                                                     cGGCTTCGCATGGCGACGAAAAATATGCGCGACATGCCAGCTATCCTTGCGGTTAAGTACATACGGCAAATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGTCCTTTTTaa                                                                              >  2:151687/1‑140 (MQ=255)                                                       gCTTCGCATGGCGACGAAAAATATGCGCGACATGCCAGCTATCCTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGTCCTTTTTAAgg                                                                            <  2:1078700/140‑1 (MQ=255)                                                         ttCGCATGGCGACGAAAAATATGCGCGACATGCCAGCTATCCTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGTCCTTTTTAAggtg                                                                          <  1:530160/140‑1 (MQ=255)                                                         ttCGCATGGCGACGAAAAATATGCGCGACATGCCAGCTATCCTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGTCCTTTTTAAggtg                                                                          <  1:738180/140‑1 (MQ=255)                                                         ttCGCATGGCGACGAAAAATATGCGCGACATGCCAGCTATCCTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGTCCTTTTTAAggtg                                                                          <  1:810111/140‑1 (MQ=255)                                                          tCGCATGGCGACGAAAAATATGCGCGACATGCCAGCTATCCTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGTCCTTTTTAAggtgg                                                                         <  2:928491/140‑1 (MQ=255)                                                          tCGCATGGCGACGAAAAATATGCGCGACATGCCAGCTATCCTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGTCCTTTTTAAggtgg                                                                         <  2:680738/140‑1 (MQ=255)                                                          tCGCATGGCGACGAAAAATATGCGCGACATGCCAGCTATCCTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGTCCTTTTTAAggtgg                                                                         <  2:1056175/140‑1 (MQ=255)                                                           cGCATGGCGACGAAAAATATGCGCGACATGCCAGCTATCCTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGTCCTTTTTAAggtggt                                                                        >  2:390040/1‑140 (MQ=255)                                                            gCATGGCGACGAAAAATATGCGCGACATGCCAGCTATCCTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGTCCTTTTTAAGGTGGtt                                                                       <  1:256695/140‑1 (MQ=255)                                                                             atGCGCGACATGCCAGCTATCCTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGTCCTTTTTAAGGTGGTTATTTACACCTTAGCGCa                                                      <  2:466392/140‑1 (MQ=255)                                                                             atGCGCGACATGCCAGCTATCCTTGCGGTTAAGTACATACGGCAAATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGTCCTTTTTAAGGTGGTTATTTACACCTTAGCGCa                                                      <  1:151687/140‑1 (MQ=255)                                                                                 gcgACATGCCAGCTATCCTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGTCCTTTTTAAGGTGGTTATTTACACCTTAGCGCAAAGc                                                  <  1:319011/140‑1 (MQ=255)                                                                                    aCATGCCAGCTATCCTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGTCCTTTTTAAGGTGGTTATTTACACCTTAGCGCAAAGCGGa                                               >  1:834901/1‑140 (MQ=255)                                                                                      aTGCCAGCTATCCTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGTCCTTTTTAAGGTGGTTATTTACACCTTAGCGCAAAGCGGACg                                             <  2:834901/140‑1 (MQ=255)                                                                                        gCCAGCTATCCTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGTCCTTTTTAAGGTGGTTATTTACACCTTAGCGCAAAGCGGACGTg                                           >  1:430928/1‑140 (MQ=255)                                                                                          cAGCTATCCTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGTCCTTTTTAAGGTGGTTATTTACACCTTAGCGCAAAGCGGACGTGGt                                         <  2:820023/140‑1 (MQ=255)                                                                                               aTCCTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGTCCTTTTTAAGGTGGTTATTTACACCTTAGCGCAAAGCGGAc                                              >  2:52991/1‑130 (MQ=255)                                                                                               aTCCTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGTCCTTTTTAAGGTGGTTATTTACACCTTAGCGCAAAGCGGAc                                              <  1:52991/130‑1 (MQ=255)                                                                                                 ccTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGTCCTTTTTAAGGTGGTTATTTACACCTTAGCGCAAAGCGGACGTg                                           >  2:325655/1‑131 (MQ=255)                                                                                                 ccTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGTCCTTTTTAAGGTGGTTATTTACACCTTAGCGCAAAGCGGACGTg                                           <  1:325655/131‑1 (MQ=255)                                                                                                    tGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGTCCTTTTTAAGGTGGTTATTTACACCTTAGCGCAAAGCGGACGTGGTTCCTACTGGa                               >  2:643445/1‑140 (MQ=255)                                                                                                      cGGTTAAGTACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGTCCTTTTTAAGGTGGTTATTTACACCTTAGCGCAAAGCGGACGTGGTTCCTACTGGAGt                             >  2:891839/1‑140 (MQ=255)                                                                                                           aaGTACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGTCCTTTTTAAGGTGGTTATTTACACCTTAGCGCAAAGCGGACGTGGTTCCTACTGGAGTGCGCa                        >  1:972744/1‑140 (MQ=255)                                                                                                              tACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGTCCTTTTTAAGGTGGTTATTTACACCTTAGCGCAAAGCGGACGTGGTTCCTACTGGAGt                             <  2:881489/132‑1 (MQ=255)                                                                                                              tACATACGGCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGTCCTTTTTAAGGTGGTTATTTACACCTTAGCGCAAAGCGGACGTGGTTCCTACTGGAGt                             >  1:881489/1‑132 (MQ=255)                                                                                                                cATACGGCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGTCCTTTTTAAGGTGGTTATTTACACCTTAGCGCAAAGCGGACGTGGTTCCTACTGGAGTGCGCATAACg                   <  1:566182/140‑1 (MQ=255)                                                                                                                cATACGGCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGTCCTTTTTAAGGTGGTTATTTACACCTTAGCGCAAAGCGGACGTGGTTCCTACTGGAGTGCGCATAACg                   <  1:658867/140‑1 (MQ=255)                                                                                                                 aTACGGCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGTCCTTTTTAAGGTGGTTATTTACACCTTAGCGCAAAGCGGACGTGGTTCCTACTGGAGTGCGCATAACGa                  >  2:59110/1‑140 (MQ=255)                                                                                                                  tACGGCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGTCCTTTTTAAGGTGGTTATTTACACCTTAGCGCAAAGCGGACGTGGTTCCTACTGGAGTGCGCATAACGaa                 >  2:62383/1‑140 (MQ=255)                                                                                                                  tACGGCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGTCCTTTTTAAGGTGGTTATTTACACCTTAGCGCAAAGCGGACGTGGTTCCTACTGGAGTGCGCATAACGaa                 <  1:59110/140‑1 (MQ=255)                                                                                                                     ggCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGTCCTTTTTAAGGTGGTTATTTACACCTTAGCGCAAAGCGGACGTGGTTCCTACTGGAg                              >  2:576575/1‑124 (MQ=255)                                                                                                                     ggCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGTCCTTTTTAAGGTGGTTATTTACACCTTAGCGCAAAGCGGACGTGGTTCCTACTGGAg                              <  1:576575/124‑1 (MQ=255)                                                                                                                     ggCAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGACTTCGTCCTTTTTAAGGTGGTTATTTACACCTTAGCGCAAAGCGGACGTGGTTCCTACTGGAGTGCGCATAACGAacac              >  1:446260/1‑140 (MQ=255)                                                                                                                       cAGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGTCCTTTTTAAGGTGGTTATTTACACCTTAGCGCAAAGCGGACGTGGTTCCTACTGGAGCGCGCATAACGAACACaa            >  1:364720/1‑140 (MQ=255)                                                                                                                        aGATGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGTCCTTTTTAAGGTGGTTATTTACACCTTAGCGCAAAGCGGACGTGGTTCCTACTGGAGTGCGCATAACGAACACAAg           <  1:882808/140‑1 (MQ=255)                                                                                                                          aTGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGTCCTTTTTAAGGTGGTTATTTACACCTTAGCGCAAAGCGGACGTGGTTCCTACTGGAGTGCGCATAACGAACACAAGCa         >  1:42978/1‑140 (MQ=255)                                                                                                                          aTGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGTCCTTTTTAAGGTGGTTATTTACACCTTAGCGCAAAGCGGACGTGGTTCCTACTGGAGTGCGCATAACGAACACAAGCa         >  2:352822/1‑140 (MQ=255)                                                                                                                          aTGGTAACGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGTCCGTTTTAAGGTGGTTATTTACACCTTAGCGCAAGGCGGACGTGGTTCCTACTGGAGTGCGCATAACGAACACAAGCa         <  2:144393/140‑1 (MQ=255)                                                                                                                                aCGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGTCCTTTTTAAGGTGGTTATTTACACCTTAGCGCAAAGCGGACGTGGTTCCTACTGGAGTGCGCATAACGAACACAAGCACTCCCg   <  1:643445/140‑1 (MQ=255)                                                                                                                                aCGGGTGGCGCTTTTACCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGTCCTTTTTAAGGTGGTTATTTACACCTTAGCGCAAAGCGGACGTGGTTCCTACTGGAGTGCGCATAACGAACACAAGCACTCCCg   >  2:1070246/1‑140 (MQ=255)                                                                                                                                 cGGGTGGCGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGTCCTTTTTAAGGTGGTTATTTACACCTTAGCGCAAAGCGGACGTGGTTCCTACTGGAGTGCGCATAACGAACACAAGCACTCCCGt  >  2:235081/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                     CGTCGAGATCGCTGGTGAAATGGATATGGGAATAAATGGGAGCCTGTTCTTCCGGCTTCGCATGGCGACGAAAAATATGCGCGACATGCCAGCTATCCTTGCGGTTAAGTACATACGGCAGATGGTAACGGGTGGTGCTTTTGCCGAAGCCAATAAAGGCGCAGTTGATGACCATGATTTCGTCCTTTTTAAGGTGGTTATTTACACCTTAGCGCAAAGCGGACGTGGTTCCTACTGGAGTGCGCATAACGAACACAAGCACTCCCGT  >  NC_000913/3580631‑3580898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑