Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,920,011	T→C	K131K (AAA→AAG)	atpH ←	ATP synthase F1 complex subunit delta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,920,011	0	T	C	100.0%	270.7 / NA	78	K131K (AAA→AAG)	atpH	ATP synthase F1 complex subunit delta
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (43/35);  total (43/35)

CAAGACGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACGCAGGTGAATAAACTGCTCCAGAACATCCGGGAGCGCGTTAA  >  NC_000913/3919879‑3920147                                                                                                                                     |                                                                                                                                         cAAGACGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCa                                                                                                                                   >  2:139447/1‑140 (MQ=255) cAAGACGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCa                                                                                                                                   >  2:150674/1‑140 (MQ=255) cAAGACGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCa                                                                                                                                   >  1:564115/1‑140 (MQ=255) cAAGACGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCa                                                                                                                                   >  1:373219/1‑140 (MQ=255)  aaGACGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCAt                                                                                                                                  <  1:422218/140‑1 (MQ=255)           gCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGa                                                                                                                         >  2:560861/1‑140 (MQ=255)                gcgcTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAAttt                                                                                                                    >  1:116420/1‑140 (MQ=255)                 cgcTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAAtttt                                                                                                                   >  1:627194/1‑140 (MQ=255)                   cTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTcg                                                                                                                 >  1:305486/1‑140 (MQ=255)                     cAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTcgcg                                                                                                               <  1:560861/140‑1 (MQ=255)                      aaGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGa                                                                                                              >  2:762275/1‑140 (MQ=255)                         acgacCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCt                                                                                                           <  2:373219/140‑1 (MQ=255)                         acgacCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTCTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCt                                                                                                           <  1:33280/140‑1 (MQ=255)                           gacCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCt                                                                                                           <  2:501170/138‑1 (MQ=255)                           gacCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCt                                                                                                           >  1:501170/1‑138 (MQ=255)                                cgTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTc                                                                                                    >  2:952916/1‑140 (MQ=255)                                 gTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCa                                                                                                   <  2:214060/140‑1 (MQ=255)                                  tACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCAc                                                                                                  >  1:1030299/1‑140 (MQ=255)                                       tGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGt                                                                                             >  1:1019926/1‑140 (MQ=255)                                       tGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGt                                                                                             >  1:1050127/1‑140 (MQ=255)                                         ccATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGc                                                                                           >  2:672805/1‑140 (MQ=255)                                            tCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGc                                                                                        >  1:128229/1‑140 (MQ=255)                                                 gACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCgg                                                                                   >  2:383511/1‑140 (MQ=255)                                                   ccATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTg                                                                                            <  2:85699/129‑1 (MQ=255)                                                   ccATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTg                                                                                            >  1:85699/1‑129 (MQ=255)                                                   ccATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGc                                                                                        <  2:58947/133‑1 (MQ=255)                                                   ccATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGc                                                                                        >  1:58947/1‑133 (MQ=255)                                                   ccATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGaa                                                                                 >  2:954216/1‑140 (MQ=255)                                                      tatCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATg                                                                              <  2:116420/140‑1 (MQ=255)                                                      tatCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATg                                                                              <  2:1019926/140‑1 (MQ=255)                                                            ccGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCt                                                                        >  1:692306/1‑140 (MQ=255)                                                                tCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCt                                                                    <  2:305486/140‑1 (MQ=255)                                                                 cGGATGATAACGCCTGTCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTc                                                                   >  2:522033/1‑140 (MQ=255)                                                                   gatgatAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCt                                                                    <  2:960206/137‑1 (MQ=255)                                                                   gatgatAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCt                                                                    >  1:960206/1‑137 (MQ=255)                                                                      gatAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCgg                                                              <  2:289870/140‑1 (MQ=255)                                                                         aaCGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAg                                                           >  2:489920/1‑140 (MQ=255)                                                                          aCGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGc                                                          >  2:597819/1‑140 (MQ=255)                                                                            gCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCct                                                        >  1:15230/1‑140 (MQ=255)                                                                              cTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGc                                                          <  2:975599/136‑1 (MQ=255)                                                                              cTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGc                                                          >  1:975599/1‑136 (MQ=255)                                                                              cTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCCGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCctca                                                      >  1:491691/1‑140 (MQ=255)                                                                               tGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCctcac                                                     >  2:20746/1‑140 (MQ=255)                                                                                gCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCctcact                                                    >  1:733528/1‑140 (MQ=255)                                                                                  cATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGTGGAAATGACGTCTACCTCAGCGGTAGCctcactca                                                  >  2:453603/1‑140 (MQ=255)                                                                                   aTTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCtcactcac                                                 >  1:242538/1‑140 (MQ=255)                                                                                       cAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCa                                             <  1:139447/140‑1 (MQ=255)                                                                                         gACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACg                                           <  2:1030299/140‑1 (MQ=255)                                                                                         gACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACg                                           <  2:879321/140‑1 (MQ=255)                                                                                         gACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACg                                           <  1:952916/140‑1 (MQ=255)                                                                                         gACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACg                                           <  2:1050127/140‑1 (MQ=255)                                                                                          aCTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACGc                                          >  1:1128097/1‑140 (MQ=255)                                                                                           cTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACGCa                                         <  1:876155/140‑1 (MQ=255)                                                                                              aTCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCa                                             <  1:336874/133‑1 (MQ=255)                                                                                              aTCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCa                                             >  2:336874/1‑133 (MQ=255)                                                                                              aTCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACGCa                                         >  2:206978/1‑137 (MQ=255)                                                                                              aTCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACGCa                                         <  1:206978/137‑1 (MQ=255)                                                                                              aTCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACGCAGGt                                      <  2:1128097/140‑1 (MQ=255)                                                                                               tCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACGCAGGt                                      <  1:424058/139‑1 (MQ=255)                                                                                               tCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACGCAGGt                                      >  2:424058/1‑139 (MQ=255)                                                                                                      tGCAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACGCAGGTGAATAAAc                              <  1:489920/140‑1 (MQ=255)                                                                                                        cAATTCAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACGCAGGTGAATAAACTg                            >  2:258066/1‑140 (MQ=255)                                                                                                             cAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACGCAGGTGAATAAACTGCTCCa                       >  1:1050118/1‑140 (MQ=255)                                                                                                             cAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACGCAGGTGAATAAACTGCTCCa                       >  1:610553/1‑140 (MQ=255)                                                                                                             cAGCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACGCAGGTGAATAAACTGCTCCa                       <  2:287998/140‑1 (MQ=255)                                                                                                               gCTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACGCAGGTGAATAAACTGCTCCAGa                     <  1:245957/140‑1 (MQ=255)                                                                                                                cTTAACTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACGCAGGTGAATAAACTGCTCCAGaa                    >  1:1131590/1‑140 (MQ=255)                                                                                                                    aCTTTGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACGCAGGTGAATAAACTGCTCCAGAACATc                <  1:20746/140‑1 (MQ=255)                                                                                                                        tGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACGCAGGTGAATAAACTGCTCCAGAACATCCggg            >  2:46837/1‑140 (MQ=255)                                                                                                                        tGCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACGCAGGTGAATAAACTGCTCCAGAACATCCggg            <  2:1131590/140‑1 (MQ=255)                                                                                                                         gCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACGCAGGTGAATAAACTGCTCCAGAACATCCGGGa           <  2:610553/140‑1 (MQ=255)                                                                                                                         gCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACGCAGGTGAATAAACTGCTCCAGAACATCCGGGa           <  2:15230/140‑1 (MQ=255)                                                                                                                         gCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACGCAGGTGAATAAACTGCTCCAGAACATCCGGGa           <  1:150674/140‑1 (MQ=255)                                                                                                                         gCGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACGCAGGTGAATAAACTGCTCCAGAACATCCGGGa           <  1:522033/140‑1 (MQ=255)                                                                                                                          cGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACGCAGGTGAATAAACTGCTCCAGAACATCCGGGa           <  2:121374/139‑1 (MQ=255)                                                                                                                          cGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACGCAGGTGAATAAACTGCTCCAGAACATCCGGGa           >  1:121374/1‑139 (MQ=255)                                                                                                                          cGTGACAGACGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACGCAGGTGAATAAACTGCTCCAGAACATCCGGGAg          >  2:66964/1‑140 (MQ=255)                                                                                                                                  aCGCTTTTCCATCGCAGCAGAAATTTTCGCGAGCCGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACGCAGGTGAATAAACTGCTCCAGAACATCCGGGAGCGCGTTaa  <  2:491691/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                         CAAGACGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACGCAGGTGAATAAACTGCTCCAGAACATCCGGGAGCGCGTTAA  >  NC_000913/3919879‑3920147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑