Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,044,758	T→C	I187T (ATT→ACT)	yihF →	uncharacterized protein YihF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,044,758	0	T	C	100.0%	183.2 / NA	52	I187T (ATT→ACT)	yihF	uncharacterized protein YihF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/25);  total (27/25)

CATTGCGATTCGCGTTTGGTGGCTCATTCTCTACGACATTAGATGTTGCCCCTGCAGAGTATGGAAAGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATATTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGTTGGTGAAAGCGAGTCGAAATTTAAT  >  NC_000913/4044626‑4044894                                                                                                                                     |                                                                                                                                         cATTGCGATTCGCGTTTGGTGGCTCATTCTCTACGACATTAGATGTTGCCCCTGCAGAGTATGGAAAGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGc                                                                                                                                   >  2:840355/1‑140 (MQ=255)  aTTGCGATTCGCGTTTGGTGGCTCATTCTCTACGACATTAGATGTTGCCCCTGCAGAGTATGGAAAGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCa                                                                                                                                  >  2:641682/1‑140 (MQ=255)         ttCGCGTTTGGTGGCTCATTCTCTACGACATTAGATGTTGCCCCTGCAGAGTATGGAAAGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGa                                                                                                                           >  1:1082656/1‑140 (MQ=255)         ttCGCGTTTGGTGGCTCATTCTCTACGACATTAGATGTTGCCCCTGCAGAGTATGGAAAGTTTTCTTTTGGTGAGCGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGa                                                                                                                           <  2:987154/140‑1 (MQ=255)           cgcgTTTGGTGGCTCATTCTCTACGACATTAGATGTTGCCCCTGCAGAGTATGGAAAGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAg                                                                                                                         >  1:596577/1‑140 (MQ=255)              gTTTGGTGGCTCATTCTCTACGACATTAGATGTTGCCCCTGCAGAGTATGGAAAGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGata                                                                                                                      <  1:1119603/140‑1 (MQ=255)              gTTTGGTGGCTCATTCTCTACGACATTAGATGTTGCCCCTGCAGAGTATGGAAAGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGata                                                                                                                      <  1:937808/140‑1 (MQ=255)                 tggtggCTCATTCTCTACGACATTAGATGTTGCCCCTGCAGAGTATGGAAAGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTg                                                                                                                   <  2:998449/140‑1 (MQ=255)                      gCTCATTCTCTACGACATTAGATGTTGCCCCTGCAGAGTATGGAAAGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTg                                                                                                              <  1:840355/140‑1 (MQ=255)                      gCTCATTCTCTACGACATTAGATGTTGCCCCTGCAGAGTATGGAAAGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTg                                                                                                              >  2:856187/1‑140 (MQ=255)                         cATTCTCTACGACATTAGATGTTGCCCCTGCAGAGTATGGAAAGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCaa                                                                                                           <  2:687197/140‑1 (MQ=255)                          aTTCTCTACGACATTAGATGTTGCCCCTGCAGAGTATGGAAAGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGa                                                                                                                           <  1:664767/123‑1 (MQ=255)                          aTTCTCTACGACATTAGATGTTGCCCCTGCAGAGTATGGAAAGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGa                                                                                                                           >  2:664767/1‑123 (MQ=255)                               ctACGACATTAGATGTTGCCCCTGCAGAGTATGGAAAGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCa                                                                                                     <  1:999711/140‑1 (MQ=255)                               ctACGACATTAGATGTTGCCCCTGCAGAGTATGGAAAGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCa                                                                                                     <  1:42639/140‑1 (MQ=255)                                          gATGTTGCCCCTGCAGAGTATGGAAAGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACaa                                                                                          >  2:750214/1‑140 (MQ=255)                                                gCCCCTGCAGAGTATGGAAAGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAAc                                                                                    >  2:342104/1‑140 (MQ=255)                                                 ccccTGCAGAGTATGGAAAGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACg                                                                                   >  1:663971/1‑140 (MQ=255)                                                          agTATGGAAAGTTTTCTTTTGGCGAGGGCCAGGTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGt                                                                          >  2:456242/1‑140 (MQ=255)                                                            tATGGAAAGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGttt                                                                        <  1:641682/140‑1 (MQ=255)                                                            tATGGAAAGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGttt                                                                        >  2:485921/1‑140 (MQ=255)                                                             aTGGAAAGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGtttt                                                                       <  1:360602/140‑1 (MQ=255)                                                               ggAAAGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTAc                                                                     >  2:854587/1‑140 (MQ=255)                                                                 aaaGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCa                                                                   >  1:290196/1‑140 (MQ=255)                                                                 aaaGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCa                                                                   >  2:82044/1‑140 (MQ=255)                                                                      tttCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGAt                                                              >  1:850151/1‑140 (MQ=255)                                                                        tCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGAAGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATtc                                                            >  2:326474/1‑140 (MQ=255)                                                                         cTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATtct                                                           <  1:139980/140‑1 (MQ=255)                                                                              ggTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGgc                                                      <  2:660471/140‑1 (MQ=255)                                                                                   gggCCAGTTTACTTTTAATGGTGAAGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGAt                                                 <  1:326474/140‑1 (MQ=255)                                                                                    ggCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGAtt                                                >  2:350023/1‑140 (MQ=255)                                                                                              cTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGaagaaaag                                      >  2:108692/1‑140 (MQ=255)                                                                                                    aTGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAAttt                                >  2:1019718/1‑140 (MQ=255)                                                                                                        tGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCgg                            >  2:831050/1‑140 (MQ=255)                                                                                                         gATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGt                           >  1:115199/1‑140 (MQ=255)                                                                                                         gATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGt                           <  1:854587/140‑1 (MQ=255)                                                                                                         gATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGt                           <  2:850151/140‑1 (MQ=255)                                                                                                         gATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGt                           <  1:677177/140‑1 (MQ=255)                                                                                                         gATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGt                           <  1:1110908/140‑1 (MQ=255)                                                                                                         gATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGt                           <  1:613522/140‑1 (MQ=255)                                                                                                         gATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAACAAATTTCCGGt                           <  2:1080442/140‑1 (MQ=255)                                                                                                                 ttCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGTTGGTGaaa                   >  1:251487/1‑140 (MQ=255)                                                                                                                      tGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGTTGGTGAAAGCGAg              >  2:1140394/1‑140 (MQ=255)                                                                                                                      tGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGTTGGTGAAAGCGAg              >  2:818423/1‑140 (MQ=255)                                                                                                                       gTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGTTGGTGAAAGCGAGt             >  2:365403/1‑140 (MQ=255)                                                                                                                        tCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGt                           <  2:449915/125‑1 (MQ=255)                                                                                                                        tCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGt                           >  1:449915/1‑125 (MQ=255)                                                                                                                        tCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGTTGGTGAAAGCGAGTc            <  1:1019718/140‑1 (MQ=255)                                                                                                                        tCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGTTGGTGAAAGCGAGTc            <  2:1094745/140‑1 (MQ=255)                                                                                                                            aCCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGTTGGTGAAAGCGAGTCGaaa        <  1:750214/140‑1 (MQ=255)                                                                                                                            aCCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGTTGGTGAAAGCGAGTCGaaa        >  1:632479/1‑140 (MQ=255)                                                                                                                               tGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGTTGGTGAAAGCGAGTCGAAAttt     >  1:207322/1‑140 (MQ=255)                                                                                                                                ggATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGTTGGTGAAAGCGAGTCGAAATTTa    <  1:831050/140‑1 (MQ=255)                                                                                                                                 gATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGTTGGTGAAAGCGAGTCGAAATTTaa   <  1:818423/140‑1 (MQ=255)                                                                                                                                  atacTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGTTGGTGAAAGCGAGTCGaa         <  1:549662/133‑1 (MQ=255)                                                                                                                                  atacTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGTTGGTGAAAGCGAGTCGAAATTTAAt  >  2:769780/1‑140 (MQ=255)                                                                                                                                  atacTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGTTGGTGAAAGCGAGTCGAAATTTAAt  <  2:115199/140‑1 (MQ=255)                                                                                                                                  atacTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGTTGGTGAAAGCGAGTCGAAATTTAAt  >  1:936206/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                         CATTGCGATTCGCGTTTGGTGGCTCATTCTCTACGACATTAGATGTTGCCCCTGCAGAGTATGGAAAGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATATTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGTTGGTGAAAGCGAGTCGAAATTTAAT  >  NC_000913/4044626‑4044894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑