Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,086,581	T→C	V189A (GTG→GCG)	fdhD →	sulfurtransferase for molybdenum cofactor sulfuration

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,086,581	0	T	C	100.0%	260.9 / NA	75	V189A (GTG→GCG)	fdhD	sulfurtransferase for molybdenum cofactor sulfuration
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (39/36);  total (39/36)

GACGTTTGATCTCAACAAACTGGATGATGCATTACGTCATCTCAACGATTTTCAGCCAGTGGGGCAACTGACTGGTTGTACTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGTGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGTCACAAGAAGGGGAAAGCTGGCAGCAAGGTGCGGTACTGGTTTCCAGTCGTGCCAGTTATGAAATGGTGCAAAAGTCGGCGATGTGCGGCGTA  >  NC_000913/4086444‑4086714                                                                                                                                          |                                                                                                                                      gACGTTTGATCTCAACAAACTGGATGATGCATTACGTCATCTCAACGATTTTCAGCCAGTGGGGCAACTGACTGGTTGTACTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCgg                                                                                                                                     >  1:146831/1‑140 (MQ=255)       tGATCTCAACAAACTGGATGATGCATTACGTCATCTCAACGATTTTCAGCCAGTGGGGCAACTGACTGGTTGTACTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGcc                                                                                                                               >  1:735649/1‑140 (MQ=255)        gATCTCAACAAACTGGATGATGCATTACGTCATCTCAACGATTTTCAGCCAGTGGGGCAACTGACTGGTTGTACTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCa                                                                                                                              >  2:338200/1‑140 (MQ=255)        gATCTCAACAAACTGGATGATGCATTACGTCATCTCAACGATTTTCAGCCAGTGGGGCAACTGACTGGTTGTACTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCa                                                                                                                              >  2:951966/1‑140 (MQ=255)            tcAACAAACTGGATGATGCATTACGTCATCTCAACGATTTTCAGCCAGTGGGGCAACTGACTGGTTGTACTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCAtgtg                                                                                                                          >  2:218730/1‑140 (MQ=255)            tcAACAAACTGGATGATGCATTACGTCATCTCAACGATTTTCAGCCAGTGGGGCAACTGACTGGTTGTACTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCAtgtg                                                                                                                          >  1:249436/1‑140 (MQ=255)             caacaaACTGGATGATGCATTACGTCATCTCAACGATTTTCAGCCAGTGGGGCAACTGACTGGTTGTACTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTgg                                                                                                                         >  2:1114073/1‑140 (MQ=255)              aacaaACTGGATGATGCATTACGTCATCTCAACGATTTTCAGCCAGTGGGGCAACTGACTGGTTGTACTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGgc                                                                                                                        >  1:867528/1‑140 (MQ=255)                caaACTGGATGATGCATTACGTCATCTCAACGATTTTCAGCCAGTGGGGCAACTGACTGGTTGTACTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGgcgc                                                                                                                      >  2:386862/1‑140 (MQ=255)                      ggATGATGCATTACGTCATCTCAACGATTTTCAGCCAGTGGGGCAACTGACTGGTTGTACTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACa                                                                                                                <  2:58954/140‑1 (MQ=255)                      ggATGATGCATTACGTCATCTCAACGATTTTCAGCCAGTGGGGCAACTGACTGGTTGTACTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACa                                                                                                                <  1:528382/140‑1 (MQ=255)                        atgatgCATTACGTCATCTCAACGATTTTCAGCCAGTGGGGCAACTGACTGGTTGTACTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACaaa                                                                                                              <  1:218730/140‑1 (MQ=255)                        atgatgCATCACGTCATCTCAACGATTTTCAGCCAGTGGGGCAACTGACTGGTTGTACTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACaaa                                                                                                              >  1:1065391/1‑140 (MQ=255)                             gCATTACGTCATCTCAACGATTTTCAGCCAGTGGGGCAACTGACTGGTTGTACTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGgcg                                                                                                                       >  2:501153/1‑126 (MQ=255)                             gCATTACGTCATCTCAACGATTTTCAGCCAGTGGGGCAACTGACTGGTTGTACTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGgcg                                                                                                                       <  1:501153/126‑1 (MQ=255)                                 tACGTCATCTCAACGATTTTCAGCCAGTGGGGCAACTGACTGGTTGTACTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACa                                                                                                                >  1:917260/1‑129 (MQ=255)                                 tACGTCATCTCAACGATTTTCAGCCAGTGGGGCAACTGACTGGTTGTACTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACa                                                                                                                <  2:917260/129‑1 (MQ=255)                                   cGTCATCTCAACGATTTTCAGCCAGTGGGGCAACTGACTGGTTGTACTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGgccg                                                                                                   >  1:760880/1‑140 (MQ=255)                                   cGTCATCTCAACGATTTTCAGCCAGTGGGGCAACTGACTGGTTGTACTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGgccg                                                                                                   <  2:760880/140‑1 (MQ=255)                                    gTCATCTCAACGATTTTCAGCCAGTGGGGCAACTGACTGGTTGTACTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGgccgc                                                                                                  <  2:1065391/140‑1 (MQ=255)                                    gTCATCTCAACGATTTTCAGCCAGTGGGGCAACTGACTGGTTGTACTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGgccgc                                                                                                  <  2:451368/140‑1 (MQ=255)                                    gTCATCTCAACGATTTTCAGCCAGTGGGGCAACTGACTGGTTGTACTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGgccgc                                                                                                  <  2:867528/140‑1 (MQ=255)                                      cATCTCAACGATTTTCAGCCAGTGGGGCAACTGACTGGTTGTACTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGccgccg                                                                                                <  2:146831/140‑1 (MQ=255)                                       aTCTCAACGATTTTCAGCCAGTGGGGCAACTGACTGGTTGTACTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACa                                                                                                                <  1:99856/123‑1 (MQ=255)                                       aTCTCAACGATTTTCAGCCAGTGGGGCAACTGACTGGTTGTACTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACa                                                                                                                >  2:99856/1‑123 (MQ=255)                                        tctcAACGATTTTCAGCCAGTGGGGCAACTGACTGGTTGTACTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGt                                                                                              >  2:47286/1‑140 (MQ=255)                                        tctcAACGATTTTCAGCCAGTGGGGCAACTGACTGGTTGTACTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGt                                                                                              <  1:338200/140‑1 (MQ=255)                                        tctcAACGATTTTCAGCCAGTGGGGCAACTGACTGGTTGTACTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGt                                                                                              >  1:98288/1‑140 (MQ=255)                                        tctcAACGATTTTCAGCCAGTGGGGCAACTGACTGGTTGTACTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGt                                                                                              >  1:1098478/1‑140 (MQ=255)                                         ctcAACGATTTTCAGCCAGTGGGGCAACTGACTGGTTGTACTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGTc                                                                                             <  2:911181/140‑1 (MQ=255)                                              cGATTTTCAGCCAGTGGGGCAACTGACTGGTTGTACTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGTCACaag                                                                                        <  2:409540/140‑1 (MQ=255)                                                  tttCAGCCAGTGGGGCAACTGACTGGTTGTACTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGTCACAAGAAgg                                                                                    <  1:1044831/140‑1 (MQ=255)                                                  tttCAGCCAGTGGGGCAACTGACTGGTTGTACTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGTCACAAGAAgg                                                                                    <  1:630084/140‑1 (MQ=255)                                                     cAGCCAGTGGGGCAACTGACTGGTTGTACTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGTCACAAGAAGGGGa                                                                                 <  1:653954/140‑1 (MQ=255)                                                         cAGTGGGGCAACTGACTGGTTGTACTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGTCACAAGAAGGGGAAAGc                                                                             <  1:451123/140‑1 (MQ=255)                                                           gTGGGGCAACTGACTGGTTGTACTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGTCACAAGAAGGGGAAAGCTg                                                                           >  1:1090221/1‑140 (MQ=255)                                                                gCAACTGACTGGTTGTACTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGTCACAAGAAGGGGAAAGCTGgcagc                                                                      >  2:504591/1‑140 (MQ=255)                                                                 cAACTGACTGGTTGTACTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGTCACAAGAAGGGGAAAGCTGgcagca                                                                     <  1:504591/140‑1 (MQ=255)                                                                   actgactgGTTGTACTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGTCACAAGAAGGGGAAAGCTGGCAGCAAg                                                                   >  2:636123/1‑140 (MQ=255)                                                                           gTTGTACTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGTCACAAGAAGGGGAAAGCTGGCAGCAAGGTGCGGTa                                                           <  1:990244/140‑1 (MQ=255)                                                                           gTTGTACTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGTCACAAGAAGGGGAAAGCTGGCAGCAAGGTGCGGTa                                                           <  2:362905/140‑1 (MQ=255)                                                                            ttGTACTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGTCACAAGAAGGGGAAAGCTGGCAGCAAGGTGCGGTAc                                                          <  1:636123/140‑1 (MQ=255)                                                                                 cTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGTCACAAGAAGGGGAAAGCTGGCAGCAAGGt                                                                 <  1:1019719/128‑1 (MQ=255)                                                                                 cTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGTCACAAGAAGGGGAAAGCTGGCAGCAAGGt                                                                 >  2:1019719/1‑128 (MQ=255)                                                                                 cTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGTCACAAGAAGGGGAAAGCTGGCAGCAAGGTGCGGTACTGGtt                                                     >  1:387655/1‑140 (MQ=255)                                                                                 cTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGTCACAAGAAGGGGAAAGCTGGCAGCAAGGTGCGGTACTGGtt                                                     >  1:341281/1‑140 (MQ=255)                                                                                     cgccgcTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGTCACAAGAAGGGGAAAGCTGGCAGCAAGGTGCGGTACTGGTTTCCa                                                 >  1:782387/1‑140 (MQ=255)                                                                                     cgccgcTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGTCACAAGAAGGGGAAAGCTGGCAGCAAGGTGCGGTACTGGTTTCCa                                                 >  2:534785/1‑140 (MQ=255)                                                                                      gccgcTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGTCACAAGAAGGGGAAAGCTGGCAGCAAGGTGCGGTACTGGTTTCCAg                                                <  1:386862/140‑1 (MQ=255)                                                                                       ccgcTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGTCACAAGAAGGGGAAAGCTGGCAGCAAGGTg                                                                <  1:903534/123‑1 (MQ=255)                                                                                       ccgcTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGTCACAAGAAGGGGAAAGCTGGCAGCAAGGTg                                                                >  2:903534/1‑123 (MQ=255)                                                                                          cTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGTCACAAGAAGGGGAAAGCTGGCAGCAAGGTGCGGTACTGGTTTCCAGTCGt                                            >  2:742068/1‑140 (MQ=255)                                                                                          cTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGTCACAAGAAGGGGAAAGCTGGCAGCAAGGTGCGGTACTGGTTTCCAGTCGt                                            >  2:592217/1‑140 (MQ=255)                                                                                          cTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGTCACAAGAAGGGGAAAGCCGGCAGCAAGGTGCGGTACTGGTTTCCAGTCGt                                            >  2:422034/1‑140 (MQ=255)                                                                                            gCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGTCACAAGAAGGGGAAAGCTGGCAGCAAGGTGCGGTACTGGTTTCCAGTCGTGc                                          <  2:341281/140‑1 (MQ=255)                                                                                            gCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGTCACAAGAAGGGGAAAGCTGGCAGCAAGGTGCGGTACTGGTTTCCAGTCGTGc                                          >  2:522233/1‑140 (MQ=255)                                                                                            gCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGTCACAAGAAGGGGAAAGCTGGCAGCAAGGTGCGGTACTGGTTTCCAGTCGTGc                                          >  2:1084275/1‑140 (MQ=255)                                                                                             ccTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGTCACAAGAAGGGGAAAGCTGGCAGCAAGGTGCGGTACTGGTTTCCAGTCGTGcc                                         <  1:522233/140‑1 (MQ=255)                                                                                             ccTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGTCACAAGAAGGGGAAAGCTGGCAGCAAGGTGCGGTACTGGTTTCCAGTCGTGcc                                         <  1:1084275/140‑1 (MQ=255)                                                                                                       gCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGTTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGTCACAAGAAGGGGAAAGCTGGCAGCAAGGTGCGGTACTGGTTTCCAGTCGTGCCAGTTATGaaa                               >  1:25545/1‑140 (MQ=255)                                                                                                       gCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGTCACAAGAAGGGGAAAGCTGGCAGCAAGGTGCGGTACTGGTTTCCAGTCGTGc                                          <  1:882709/129‑1 (MQ=255)                                                                                                       gCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGTCACAAGAAGGGGAAAGCTGGCAGCAAGGTGCGGTACTGGTTTCCAGTCGTGc                                          >  2:882709/1‑129 (MQ=255)                                                                                                       gCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGTCACAAGAAGGGGAAAGCTGGCAGCAAGGTGCGGTACTGGTTTCCAGTCGTGCCAGTTATGaaa                               >  1:722744/1‑140 (MQ=255)                                                                                                       gCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGTCACAAGAAGGGGAAAGCTGGCAGCAAGGTGCGGTACTGGTTTCCAGTCGTGCCAGTTATGaaa                               >  2:895472/1‑140 (MQ=255)                                                                                                       gCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGTCACAAGAAGGGGAAAGCTGGCAGCAAGGTGCGGTACTGGTTTCCAGTCGTGCCAGTTATGaaa                               >  2:187707/1‑140 (MQ=255)                                                                                                        ccATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGGGCTGGACAAACTGTTAGGCCGCCGGTCACAAGAAGGGGAAAGCTGGCAGCAAGGTGCGGTACTGGTTTCCAGTCGTGCCAGTTATGAAAt                              >  2:55976/1‑140 (MQ=255)                                                                                                         cATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGTCACAAGAAGGGGAAAGCTGGCAGCAAGGTGCGGTACTGGTTTCCAGTCGTGCCAGTTATGAAATg                             <  2:1115094/140‑1 (MQ=255)                                                                                                         cATCTGGCGAACTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGTCACAAGAAGGGGAAAGCTGGCAGCAAGGTGCGGTACTGGTTTCCAGTCGTGCCAGTTATGAAATg                             <  1:47286/140‑1 (MQ=255)                                                                                                                  aaCTGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGTCACAAGAAGGGGAAAGCTGGCAGCAAGGTGCGGTACTGGTTTCCAGTCGTGCCAGTTATGAAATGGTGCAAAAg                    <  2:722744/140‑1 (MQ=255)                                                                                                                     tGGTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGTCACAAGAAGGGGAAAGCTGGCAGCAAGGTGCGGTACTGGTTTCCAGTCGTGCCAGTTATGAAATGGTGCAAAAGTCg                 <  1:1114073/140‑1 (MQ=255)                                                                                                                       gTCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGTCACAAGAAGGGGAAAGCTGGCAGCAAGGTGCGGTACTGGTTTCCAGTCGTGCCAGTTATGAAATGGTGCAAAAGTCGGc               >  1:523207/1‑140 (MQ=255)                                                                                                                        tCGGCGGGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGCTAGGCCGCCGGTCACAAGAAGGGGAAAGCTGGCAGCAAGGTGCGGTACTGGTTTCCAGTCGTGCCAGTTATGAAATGGTGCAAAAGTCGGCg              <  1:315402/140‑1 (MQ=255)                                                                                                                          ggcggGCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGTCACAAGAAGGGGAAAGCTGGCAGCAAGGTGCGGTACTGGTTTCCAGTCGTGCCAGTTATGAAATGGTGCAAAAGTCGGCGAt            <  2:661047/140‑1 (MQ=255)                                                                                                                              ggCATGAAGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGTCACAAGAAGGGGAAAGCTGGCAGCAAGGTGCGGTACTGGTTTCCAGTCGTGCCAGTTATGAAATGGTGCAAAAGTCGGCGATGTgc        >  1:341930/1‑140 (MQ=255)                                                                                                                                    aaGACGCGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGTCACAAGAAGGGGAAAGCTGGCAGCAAGGTGCGGTACTGGTTTCCAGTCGTGCCAGTTATGAAATGGTGCAAAAGTCGGCGATGTGCGGCGTa  <  1:55976/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      GACGTTTGATCTCAACAAACTGGATGATGCATTACGTCATCTCAACGATTTTCAGCCAGTGGGGCAACTGACTGGTTGTACTCACGCCGCTGCCTGGATGTTGCCATCTGGCGAACTGGTCGGCGGGCATGAAGACGTGGGTCGCCATGTGGCGCTGGACAAACTGTTAGGCCGCCGGTCACAAGAAGGGGAAAGCTGGCAGCAAGGTGCGGTACTGGTTTCCAGTCGTGCCAGTTATGAAATGGTGCAAAAGTCGGCGATGTGCGGCGTA  >  NC_000913/4086444‑4086714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑