Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,102,693	A→G	intergenic (+20/‑129)	kdgT → / → yiiM	2‑dehydro‑3‑deoxy‑D‑gluconate:H(+) symporter/2‑amino‑6‑N‑hydroxylaminopurine resistance protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,102,693	0	A	G	100.0%	203.1 / NA	58	intergenic (+20/‑129)	kdgT/yiiM	2‑dehydro‑3‑deoxy‑D‑gluconate:H(+) symporter/2‑amino‑6‑N‑hydroxylaminopurine resistance protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/32);  total (26/32)

CTGGTAGCGACGGCGGTCATTGTGACTTCGATTCTGGTGCCAATTCTTACCTCTATCTGGTCACGTAAAGTCAAAGCCAGAGCAGCGAAAATCGAAATTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCACAGCCGGATGAACTAACGTTCGTCCGGTTTGCTATTGAGTTCCTGCCTGGATACTTACCGCGAAAACAGCACGCCTGACATAAAATAAATATGGTCTAATGGGGAAATTTCTCGTGGAGAGGGAACAGATGCGAT  >  NC_000913/4102557‑4102828                                                                                                                                         |                                                                                                                                        cTGGTAGCGACGGCGGTCATTGTGACTTCGATTCTGGTGCCAATTCTTACCTCTATCTGGTCACGTAAAGTCAAAGCCAGAGCAGCGAAAATCGAAATTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCGCAg                                                                                                                                      <  1:224741/140‑1 (MQ=255)  tGGTAGCGACGGCGGTCATTGTGACTTCGATTCTGGTGCCAATTCTTACCTCTATCTGGTCACGTAAAGTCAAAGCCAGAGCAGCGAAAATCGAAATTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCGCAGc                                                                                                                                     <  1:384954/140‑1 (MQ=255)     tAGCGACGGCGGTCATTGTGACTTCGATTCTGGTGCCAATTCTTACCTCTATCTGGTCACGTAAAGTCAAAGCCAGAGCAGCGAAAATCGAAATTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCGCAGCCgg                                                                                                                                  <  2:89094/140‑1 (MQ=255)      aGCGACGGCGGTCATTGTGACTTCGATTCTGGTGCCAATTCTTACCTCTATCTGGTCACGTAAAGTCAAAGCCAGAGCAGCGAAAATCGAAATTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCGCAGCCGGa                                                                                                                                 <  1:225116/140‑1 (MQ=255)      aGCGACGGCGGTCATTGTGACTTCGATTCTGGTGCCAATTCTTACCTCTATCTGGTCACGTAAAGTCAAAGCCAGAGCAGCGAAAATCGAAATTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCGCAGCCGGa                                                                                                                                 >  1:366303/1‑140 (MQ=255)          aCGGCGGTCATTGTGACTTCGATTCTGGTGCCAATTCTTACCTCTATCTGGTCACGTAAAGTCAAAGCCAGAGCAGCGAAAATCGAAATTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCGCAGCCGGATGaa                                                                                                                             >  2:955734/1‑140 (MQ=255)              cggTCATTGTGACTTCGATTCTGGTGCCAATTCTTACCTCTATCTGGTCACGTAAAGTCAAAGCCAGAGCAGCGAAAATCGAAATTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCGCAGCCGGATGAACTaa                                                                                                                         >  1:402470/1‑140 (MQ=255)                      gtgACTTCGATTCTGGTGCCAATTCTTACCTCTATCTGGTCACGTAAAGTCAAAGCCAGAGCAGCGAAAATCGAAATTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCGCAGCCGGATGAACTAACGTTCGTc                                                                                                                 >  2:1012491/1‑140 (MQ=255)                      gtgACTTCGATTCTGGTGCCAATTCTTACCTCTATCTGGTCACGTAAAGTCAAAGCCAGAGCAGCGAAAATCGAAATTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCGCAGCCGGATGAACTAACGTTCGTc                                                                                                                 <  2:104098/140‑1 (MQ=255)                      gtgACTTCGATTCTGGTGCCAATTCTTACCTCTATCTGGTCACGTAAAGTCAAAGCCAGAGCAGCGAAAATCGAAATTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCGCAGCCGGATGAACTAACGTTCGTc                                                                                                                 <  2:366303/140‑1 (MQ=255)                        gACTTCGATTCTGGTGCCAATTCTTACCTCTATCTGGTCACGTAAAGTCAAAGCCAGAGCAGCGAAAATCGAAATTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCGCAGCCGGATGAACTAACGTTCGTCCg                                                                                                               <  2:784397/140‑1 (MQ=255)                        gACTTCGATTCTGGTGCCAATTCTTACCTCTATCTGGTCACGTAAAGTCAAAGCCAGAGCAGCGAAAATCGAAATTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCGCAGCCGGATGAACTAACGTTCGTCCg                                                                                                               <  1:493055/140‑1 (MQ=255)                          cTTCGATTCTGGTGCCAATTCTTACCTCTATCTGGTCACGTAAAGTCAAAGCCAGAGCAGCGAAAATCGAAATTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCGCAGCCGGATGAACTAACGTTCGTCCGGt                                                                                                             >  2:975367/1‑140 (MQ=255)                          cTTCGATTCTGGTGCCAATTCTTACCTCTATCTGGTCACGTAAAGTCAAAGCCAGAGCAGCGAAAATCGAAATTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCGCAGCCGGATGAACTAACGTTCGTCCGGt                                                                                                             <  2:402470/140‑1 (MQ=255)                                         cAATTCTTACCTCTATCTGGTCACGTAAAGTCAAAGCCAGAGCAGCGAAAATCGAAATTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCGCAGCCGGATGAACTAACGTTCGTCCGGTTTGCTATTGAGTTcc                                                                                              <  1:975367/140‑1 (MQ=255)                                             tCTTACCTCTATCTGGTCACGTAAAGTCAAAGCCAGAGCAGCGAAAATCGAAATTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCGCAGCCGGATGAACTAACGTTCGTCCGGTTTGCTATTGGGTTcctgcc                                                                                          <  2:14912/140‑1 (MQ=255)                                                tACCTCTATCTGGTCACGTAAAGTCAAAGCCAGAGCAGCGAAAATCGAAATTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCGCAGCCGGATGAACTAACGTTCGTCCGGTTTGCTATTGAGTTCCTGCCTgg                                                                                       >  1:1078160/1‑140 (MQ=255)                                                      tATCTGGTCACGTAAAGTCAAAGCCAGAGCAGCGAAAATCGAAATTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCGCAGCCGGATGAACTAACGTTCGTCCGGTTTGCTATTGAGTTcc                                                                                              >  2:1043418/1‑127 (MQ=255)                                                      tATCTGGTCACGTAAAGTCAAAGCCAGAGCAGCGAAAATCGAAATTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCGCAGCCGGATGAACTAACGTTCGTCCGGTTTGCTATTGAGTTcc                                                                                              <  1:1043418/127‑1 (MQ=255)                                                       aTCTGGTCACGTAAAGTCAAAGCCAGAGCAGCGAAAATCGAAATTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCGCAGCCGGATGAACTAACGTTCGTCCGGTTTGCTATTGAGTTCCTGCCTGGATACTTa                                                                                >  2:26520/1‑140 (MQ=255)                                                       aTCTGGTCACGTAAAGTCAAAGCCAGAGCAGCGAAAATCGAAATTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCGCAGCCGGATGAACTAACGTTCGTCCGGTTTGCTATTGAGTTCCTGCCTGGATACTTa                                                                                >  2:842731/1‑140 (MQ=255)                                                        tCTGGTCACGTAAAGTCAAAGCCAGAGCAGCGAAAATCGAAATTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCGCAGCCGGATGAACTAACGTTCGTCCGGTTTGCTATTGAGTTCCTGCCTGGATACTTAc                                                                               >  1:23330/1‑140 (MQ=255)                                                        tCTGGTCACGTAAAGTCAAAGCCAGAGCAGCGAAAATCGAAATTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCGCAGCCGGATGAACTAACGTTCGTCCGGTTTGCTATTGAGTTCCTGCCTGGATACTTAc                                                                               >  1:854401/1‑140 (MQ=255)                                                            gTCACGTAAAGTCAAAGCCAGAGCAGCGAAAATCGAAATTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCGCAGCCGGATGAACTAACGTTCGTCCGGTTTGCTATTGAGTTCCTGCCTGGATACTTACcgcg                                                                           >  2:650561/1‑140 (MQ=255)                                                             tCACGTAAAGTCAAAGCCAGAGCAGCGAAAATCGAAATTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCGCAGCCGGATGAACTAACGTTCGTCCGGTTTGCTATTGAGTTCCTGCCTGGATACTTACCGCGa                                                                          >  2:468514/1‑140 (MQ=255)                                                             tCACGTAAAGTCAAAGCCAGAGCAGCGAAAATCGAAATTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCGCAGCCGGATGAACTAACGTTCGTCCGGTTTGCTATTGAGTTCCTGCCTGGATACTTACCGCGa                                                                          >  2:65126/1‑140 (MQ=255)                                                                cGTAAAGTCAAAGCCAGAGCAGCGAAAATCGAAATTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCGCAGCCGGATGAACTAACGTTCGTCCGGTTTGCTATTGAGTTCCTGCCTGGATACTTACCGCGaaaa                                                                       >  1:413982/1‑140 (MQ=255)                                                                cGTAAAGTCAAAGCCAGAGCAGCGAAAATCGAAATTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCGCAGCCGGATGAACTAACGTTCGTCCGGTTTGCTATTGAGTTCCTGCCTGGATACTTACCGCGaaaa                                                                       >  2:861031/1‑140 (MQ=255)                                                                  tAAAGTCAAAGCCAGAGCAGCGAAAATCGAAATTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCGCAGCCGGATGAACTAACGTTCGTCCGGTTTGCTATTGAGTTCCTGCCTGGATACTTACCGCGAAAACa                                                                     >  2:293945/1‑140 (MQ=255)                                                                      gTCAAAGCCAGAGCAGCGAAAATCGAAATTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCGCAGCCGGATGAACTAACGTTCGTCCGGTTTGCTATTGAGTTCCTGCCTGGATACTTACCGCGAAAACAGCAc                                                                 >  1:574792/1‑140 (MQ=255)                                                                         aaaGCCAGAGCAGCGAAAATCGAAATTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCGCAGCCGGATGAACTAACGTTCGTCCGGTTTGCTATTGAGTTCCTGCCTGGATACTTACCGCGAAAACAGCACGcc                                                              <  1:861031/140‑1 (MQ=255)                                                                         aaaGCCAGAGCAGCGAAAATCGAAATTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCGCAGCCGGATGAACTAACGTTCGTCCGGTTTGCTATTGAGTTCCTGCCTGGATACTTACCGCGAAAACAGCACGcc                                                              <  2:1078160/140‑1 (MQ=255)                                                                           aGCCAGAGCAGCGAAAATCGAAATTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCGCAGCCGGATGAACTAACGTTCGTCCGGTTTGCTATTGAGTTCCTGCCTGGATACTTACCGCGAAAACAGCACGCCTg                                                            >  2:146591/1‑140 (MQ=255)                                                                              cAGAGCAGCGAAAATCGAAATTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCGCAGCCGGATGAACTAACGTTCGTCCGGTTTGCTATTGAGTTCCTGCCTGGATACTTACCGCGAAAACAGCACGCCTGACa                                                         <  1:592693/140‑1 (MQ=255)                                                                              cAGAGCAGCGAAAATCGAAATTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCGCAGCCGGATGAACTAACGTTCGTCCGGTTTGCTATTGAGTTCCTGCCTGGATACTTACCGCGAAAACAGCACGCCTGACa                                                         <  1:1012491/140‑1 (MQ=255)                                                                              cAGAGCAGCGAAAATCGAAATTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCGCAGCCGGATGAACTAACGTTCGTCCGGTTTGCTATTGAGTTCCTGCCTGGATACTTACCGCGAAAACAGCACGCCTGACa                                                         <  1:146591/140‑1 (MQ=255)                                                                               agagCAGCGAAAATCGAAATTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCGCAGCCGGATGAACTAACGTTCGTCCGGTTTGCTATTGAGTTCCTGCCTGGATACTTACCGCGAAAACAGCACGCCTGACat                                                        <  1:955734/140‑1 (MQ=255)                                                                               agagCAGCGAAAATCGAAATTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCGCAGCCGGATGAACTAACGTTCGTCCGGTTTGCTATTGAGTTCCTGCCTGGATACTTACCGCGAAAACAGCACGCCTGACat                                                        <  2:574792/140‑1 (MQ=255)                                                                                 agcagcGAAAATCGAAATTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCGCAGCCGGATGAACTAACGTTCGTCCGGTTTGCTATTGAGTTCCTGCCTGGATACTTACCGCGAAAACAGCACGCCTGACataa                                                      >  1:791000/1‑140 (MQ=255)                                                                                    agcGAAAATCGAAATTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCGCAGCCGGATGAACTAACGTTCGTCCGGTTTGCTATTGAGTTCCTGCCTGGATACTTACCGCGAAAACAGCACGCCTGACataaaat                                                   <  1:650561/140‑1 (MQ=255)                                                                                        aaaaTCGAAATTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCGCAGCCGGATGAACTAACGTTCGTCCGGTTTGTTATTGAGTTCCTGCCTGGATACTTACCGCGAAAACAGCACGCCTGACATAaaataaat                                               <  2:505207/140‑1 (MQ=255)                                                                                         aaaTCGAAATTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCGCAGCCGGATGAACTAACGTTCGTCCGGTTTGCTATTGAGTTCCTGCCTGGATACTTACCGCGAAAACAGCACGCCTGACATAAaataaata                                              <  2:854401/140‑1 (MQ=255)                                                                                         aaaTCGAAATTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCGCAGCCGGATGAACTAACGTTCGTCCGGTTTGCTATTGAGTTCCTGCCTGGATACTTACCGCGAAAACAGCACGCCTGACATAAaataaata                                              <  1:293945/140‑1 (MQ=255)                                                                                                aaTTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCGCAGCCGGATGAACTAACGTTCGTCCGGTTTGCTATTGAGTTCCTGCCTGGATACTTACCGCGAAAACTGCACGCCTGACa                                                         >  2:703739/1‑122 (MQ=255)                                                                                                aaTTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCGCAGCCGGATGAACTAACGTTCGTCCGGTTTGCTATTGAGTTCCTGCCTGGATACTTACCGCGAAAACAGCACGCCTGACa                                                         >  2:127747/1‑122 (MQ=255)                                                                                                aaTTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCGCAGCCGGATGAACTAACGTTCGTCCGGTTTGCTATTGAGTTCCTGCCTGGATACTTACCGCGAAAACAGCACGCCTGACa                                                         <  1:127747/122‑1 (MQ=255)                                                                                                aaTTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCGCAGCCGGATGAACTAACGTTCGTCCGGTTTGCTATTGAGTTCCTGCCTGGATACTTACCGCGAAAACAGCACGCCTGACa                                                         <  1:703739/122‑1 (MQ=255)                                                                                                                 aaTAAACCTGCTTCCTTAATCCCCGCAGCCGGATGAACTAACGTTCGTCCGGTTTGCTATTGAGTTCCTGCCTGGATACTTACCGCGAAAACAGCACGCCTGACATAAAATAAATATGGTCTAATGGGGAAATTTCTCGt                      <  1:65126/140‑1 (MQ=255)                                                                                                                  aTAAACCTGCTTCCTTAATCCCCGCAGCCGGATGAACTAACGTTCGTCCGGTTTGCTATTGAGTTCCTGCCTGGATACTTACCGCGAAAACAGCGCGCCTGACATAAAATAAATATGGTCTAATGGGGAAATTTCTCGTg                     <  1:26520/140‑1 (MQ=255)                                                                                                                      aCCTGCTTCCTTAATCCCCGCAGCCGGATGAACTAACGTTCGTCCGGTTTGCTATTGAGTTCCTGCCTGGGTACTTACCGCGAAAACAGCACGCCTGACATAAAATAAATATGGTCTAATGGGGAAATTTCTCGTGgaga                 <  2:442976/140‑1 (MQ=255)                                                                                                                      aCCTGCTTCCTTAATCCCCGCAGCCGGATGAACTAACGTTCGTCCGGTTTGCTATTGAGTTCCTGCCTGGATACTTACCGCGAAAACAGCACGCCTGACATAAAATAAATATGGTCTAATGGGGAAATTTCTCGt                      <  2:608820/135‑1 (MQ=255)                                                                                                                      aCCTGCTTCCTTAATCCCCGCAGCCGGATGAACTAACGTTCGTCCGGTTTGCTATTGAGTTCCTGCCTGGATACTTACCGCGAAAACAGCACGCCTGACATAAAATAAATATGGTCTAATGGGGAAATTTCTCGt                      >  1:608820/1‑135 (MQ=255)                                                                                                                           cTTCCTTAATCCCCGCAGCCGGATGAACTAACGTTCGTCCGGTTTGCTATTGAGTTCCTGCCTGGATACTTACCGCGAAAACAGCACGCCTGACATAAAATAAATATGGTCTAATGGGGAAATTTCTCGTGGAGAGGGaa            <  2:1091723/140‑1 (MQ=255)                                                                                                                                 tAATCCCCGCAGCCGGATGAACTAACGTTCGTCCGGTTTGCTATTGAGTTCCTGCCTGGATACTTACCGCGAAAACAGCACGCCTGACATAAAATAAATATGGTCTAATGGGGAAATTTCTCGTGGAGAGGGAACAGATg      <  2:812954/140‑1 (MQ=255)                                                                                                                                 tAATCCCCGCAGCCGGATGAACTAACGTTCGTCCGGTTTGCTATTGAGTTCCTGCCTGGATACTTACCGCGAAAACAGCACGCCTGACATAAAATAAATATGGTCTAATGGGGAAATTTCTCGTGGAGAGGGAACAGATg      >  2:853710/1‑140 (MQ=255)                                                                                                                                     ccccGCAGCCGGATGAACTAACGTTCGTCCGGTTTGCTATTGAGTTCCTGCCTGGATACTTACCGCGAAAACAGCACGCCTGACATAAAATAAATATGGTCTAATGGGGAAATTTCTCGTGGAGAGGGAACa          >  2:680984/1‑132 (MQ=255)                                                                                                                                     ccccGCAGCCGGATGAACTAACGTTCGTCCGGTTTGCTATTGAGTTCCTGCCTGGATACTTACCGCGAAAACAGCACGCCTGACATAAAATAAATATGGTCTAATGGGGAAATTTCTCGTGGAGAGGGAACa          <  1:680984/132‑1 (MQ=255)                                                                                                                                     ccccGCAGCCGGATGAACTAACGTTCGTCCGGTTTGCTATTGAGTTCCTGCCTGGATACTTACCGCGAAAACAGCACGCCTGACATAAAATAAATATGGTCTAATGGGGAAATTTCTCGTGGAGAGGGAACAGATGCGat  >  2:659204/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        CTGGTAGCGACGGCGGTCATTGTGACTTCGATTCTGGTGCCAATTCTTACCTCTATCTGGTCACGTAAAGTCAAAGCCAGAGCAGCGAAAATCGAAATTTTAGGTACGGTGAAATAAACCTGCTTCCTTAATCCCCACAGCCGGATGAACTAACGTTCGTCCGGTTTGCTATTGAGTTCCTGCCTGGATACTTACCGCGAAAACAGCACGCCTGACATAAAATAAATATGGTCTAATGGGGAAATTTCTCGTGGAGAGGGAACAGATGCGAT  >  NC_000913/4102557‑4102828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑