Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,110,997	C→T	A171T (GCA→ACA)	tpiA ←	triose‑phosphate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,110,997	0	C	T	100.0%	240.0 / NA	67	A171T (GCA→ACA)	tpiA	triose‑phosphate isomerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (41/26);  total (41/26)

CAGAGCCGCCGTACTGAATGATCACTTGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGTGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCAC  >  NC_000913/4110871‑4111133                                                                                                                               |                                                                                                                                         cAGAGCCGCCGTACTGAATGATCACTTGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGTGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGTCCATACAGGTTCg                                                                                                                             >  2:51327/1‑140 (MQ=255) cAGAGCCGCCGTACTGAATGATCACTTGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGTGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGTCCATACAGGTTCg                                                                                                                             <  2:162570/140‑1 (MQ=255)  agagCCGCCGTACTGAATGATCACTTGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGTGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGTCCATACAGGTTCGt                                                                                                                            <  1:951746/140‑1 (MQ=255)          cgTACTGAATGATCACTTGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGTGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATa                                                                                                                    >  2:541366/1‑140 (MQ=255)                      tCACTTGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGTGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCg                                                                                                        >  2:1084768/1‑140 (MQ=255)                      tCACTTGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGTGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCg                                                                                                        >  1:488839/1‑140 (MQ=255)                        aCTTGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGTGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGaa                                                                                                      >  1:335524/1‑140 (MQ=255)                             ttCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGTGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCg                                                                                                 >  1:266221/1‑140 (MQ=255)                              tCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGTGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCgc                                                                                                <  1:569845/140‑1 (MQ=255)                               cAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGTGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCgca                                                                                               >  2:852934/1‑140 (MQ=255)                                ggCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGTGCCTGAGCCGGAGTTGCAGATTTGCCGGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCATCTTCGAATGCCgcag                                                                                              >  1:137171/2‑140 (MQ=255)                                  cGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGTGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCgcagca                                                                                            >  2:506172/1‑140 (MQ=255)                                      gTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGTGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCt                                                                                        <  2:335524/140‑1 (MQ=255)                                           cGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGTGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGtt                                                                                   >  2:557085/1‑140 (MQ=255)                                            gTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGTGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCgcag                                                                                              <  2:659394/128‑1 (MQ=255)                                            gTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGTGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCgcag                                                                                              >  1:659394/1‑128 (MQ=255)                                            gTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGTGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGttt                                                                                  >  2:168167/1‑140 (MQ=255)                                            gTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGTGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGttt                                                                                  >  1:921148/1‑140 (MQ=255)                                             tCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGTGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGtttt                                                                                 <  1:372270/140‑1 (MQ=255)                                               aaCTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGTGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCa                                                                               >  2:386018/1‑140 (MQ=255)                                               aaCTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGTGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCa                                                                               >  2:110031/1‑140 (MQ=255)                                                     aGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGTGCCTGAGCCGGAGTTGTAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTa                                                                            >  1:539587/1‑137 (MQ=255)                                                     aGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGTGCCTGAGCCGGAGTTGTAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTa                                                                            <  2:539587/137‑1 (MQ=255)                                                     aGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGTGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGACAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACcg                                                                         <  1:127706/140‑1 (MQ=255)                                                           gtgGTCACGGATGACTTTGTGAACAGCCTGTGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGACTGCCGCAGCACCCTGAGATTTCAGTACCGCGCCGa                                                                   >  2:827049/1‑140 (MQ=255)                                                           gtgGTCACGGATGAATTTGTGAACAGCCTGTGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACcgcg                                                                       >  1:1101244/1‑136 (MQ=255)                                                           gtgGTCACGGATGAATTTGTGAACAGCCTGTGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACcgcg                                                                       <  2:1101244/136‑1 (MQ=255)                                                            tgGTCACGGATGAATTTGTGAACAGCCTGTGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGAt                                                                  >  1:538594/1‑140 (MQ=255)                                                                        aaTATGTGAACAGCCTGTGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCa                                                      >  1:1098766/1‑140 (MQ=255)                                                                         aTTTGTGAACAGCCTGTGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCaa                                                     >  2:1056761/1‑140 (MQ=255)                                                                          tttGTGAACAGCCTGTGCCTGAGCCGGAGTTGCAGATTTGCCGGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCaaa                                                    <  2:137171/140‑1 (MQ=255)                                                                                     ccTGTGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCAAActtc                                                <  2:516237/133‑1 (MQ=255)                                                                                     ccTGTGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCAAActtc                                                >  1:516237/1‑133 (MQ=255)                                                                                     ccTGTGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCAAACTTCTTCAGtt                                         >  1:168676/1‑140 (MQ=255)                                                                                     ccTGTGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCAAACTTCTTCAGtt                                         >  2:821580/1‑140 (MQ=255)                                                                                        gtgCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCAAACTTCTTCAGTTTTg                                      >  2:530496/1‑140 (MQ=255)                                                                                        gtgCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCAAACTTCTTCAGTTTTg                                      <  2:925109/140‑1 (MQ=255)                                                                                        gtgCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCAAACTTCTTCAGTTTTg                                      >  2:544025/1‑140 (MQ=255)                                                                                          gCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCAAACTTCTTCAGTTTTGcc                                    <  1:544025/140‑1 (MQ=255)                                                                                           ccTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCAAACTTCTTCAGTTTTGccc                                   <  2:921148/140‑1 (MQ=255)                                                                                               aGCCGGAGTTGCAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTGAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCtt                               >  2:548238/1‑140 (MQ=255)                                                                                               aGCCGGAGTTGCAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCtt                               >  2:817696/1‑140 (MQ=255)                                                                                               aGCCGGAGTTGCAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCtt                               >  2:302476/1‑140 (MQ=255)                                                                                               aGCCGGAGTTGCAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCtt                               >  2:1077497/1‑140 (MQ=255)                                                                                               aGCCGGAGTTGCAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCtt                               >  2:1012450/1‑140 (MQ=255)                                                                                               aGCCGGAGTTGCAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCtt                               >  2:52481/1‑140 (MQ=255)                                                                                               aGCCGGAGTTGCAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCtt                               >  2:52841/1‑140 (MQ=255)                                                                                                   ggAGTTGCAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGGACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCAtt                           >  2:710416/1‑140 (MQ=255)                                                                                                     aGTTGCAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCAtttt                         >  2:1101449/1‑140 (MQ=255)                                                                                                     aGTTGCAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCAtttt                         <  2:538594/140‑1 (MQ=255)                                                                                                         gCAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGc                     <  2:266221/140‑1 (MQ=255)                                                                                                         gCAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGc                     <  1:168167/140‑1 (MQ=255)                                                                                                          cAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCt                    <  1:1012450/140‑1 (MQ=255)                                                                                                          cAGATTTGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCt                    >  1:945014/1‑140 (MQ=255)                                                                                                               ttGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCGTTTTCAGCTTCGGt               >  2:290847/1‑140 (MQ=255)                                                                                                               ttGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGt               >  1:818387/1‑140 (MQ=255)                                                                                                               ttGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGt               <  1:1084768/140‑1 (MQ=255)                                                                                                               ttGCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGt               >  2:168017/1‑140 (MQ=255)                                                                                                                 gCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGttt             >  2:688900/1‑140 (MQ=255)                                                                                                                 gCCAGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGttt             <  1:1056761/140‑1 (MQ=255)                                                                                                                    aGTACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCAc          <  1:52841/140‑1 (MQ=255)                                                                                                                      tACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCg        <  1:530496/140‑1 (MQ=255)                                                                                                                      tACCGATTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCg        <  1:821580/140‑1 (MQ=255)                                                                                                                           aTTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCAcc         <  1:711403/134‑1 (MQ=255)                                                                                                                           aTTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCAc          >  1:132859/1‑133 (MQ=255)                                                                                                                           aTTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCAc          <  2:132859/133‑1 (MQ=255)                                                                                                                           aTTGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTAGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCAcc         >  2:711403/1‑134 (MQ=255)                                                                                                                            ttGTCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGcac  <  1:1101449/140‑1 (MQ=255)                                                                                                                               |                                                                                                                                         CAGAGCCGCCGTACTGAATGATCACTTGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGTGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCAAACTTCTTCAGTTTTGCCCGCTTCATTTTCAGCTTCGGTTTCACCGATGCAC  >  NC_000913/4110871‑4111133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑