BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,247,760	A→G	N326S (AAC→AGC)	malK →	maltose ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,247,760	0	A	G	100.0%	368.7 / NA	105	N326S (AAC→AGC)	malK	maltose ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (55/50); total (55/50)

Only 100 of 106 total aligned reads displayed.

ATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAACCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCAGAGCGTTGCCATCTGTTCCGTGAGGATGGCACTGCATGTCGTCGACTGCATAAGGAGCCGGGCGT  >  NC_000913/4247624‑4247895

atGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCt                                                                                                                                      <  1:761961/140‑1 (MQ=255)

     gCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGgtgt                                                                                                                                 <  1:42901/140‑1 (MQ=255)

       tGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTAc                                                                                                                               <  2:114705/140‑1 (MQ=255)

           tATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGcc                                                                                                                           <  1:437734/140‑1 (MQ=255)

                 cccGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAacg                                                                                                                     <  2:484064/140‑1 (MQ=255)

                   cGGAACATCTACTGCCGAGTGATATCGCTGCCGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAacgac                                                                                                                   <  2:803988/140‑1 (MQ=255)

                   cGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAacgac                                                                                                                   >  2:819823/1‑140 (MQ=255)

                     gAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACgt                                                                                                                 >  2:262008/1‑140 (MQ=255)

                        cATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACgtggt                                                                                                              <  1:819823/140‑1 (MQ=255)

                        cATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACgtggt                                                                                                              <  1:262008/140‑1 (MQ=255)

                         aTCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACgtggtg                                                                                                             <  1:273274/140‑1 (MQ=255)

                         aTCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACgtggtg                                                                                                             <  1:966887/140‑1 (MQ=255)

                          tCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGgtgt                                                                                                            >  2:1137466/1‑140 (MQ=255)

                          tCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGgtgt                                                                                                            >  1:786222/1‑140 (MQ=255)

                            tACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTg                                                                                                          <  1:169324/140‑1 (MQ=255)

                                gCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTag                                                                                                      <  1:346197/140‑1 (MQ=255)

                                gCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTag                                                                                                      >  2:420322/1‑140 (MQ=255)

                                    aGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAacgac                                                                                                                   >  1:998813/1‑123 (MQ=255)

                                    aGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAacgac                                                                                                                   <  2:998813/123‑1 (MQ=255)

                                    aGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACgtggt                                                                                                              <  2:424558/128‑1 (MQ=255)

                                    aGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTagaaga                                                                                                  >  1:892715/1‑140 (MQ=255)

                                    aGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAACCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTagaaga                                                                                                  >  1:1056583/1‑140 (MQ=255)

                                     gTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAacgacg                                                                                                                  <  1:96452/123‑1 (MQ=255)

                                     gTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAacgacg                                                                                                                  >  2:96452/1‑123 (MQ=255)

                                      tGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGaagaag                                                                                                >  2:181866/1‑140 (MQ=255)

                                        atatCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGt                                                                                              >  2:911174/1‑140 (MQ=255)

                                         tatCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTg                                                                                             <  1:1093100/140‑1 (MQ=255)

                                         tatCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTg                                                                                             <  1:1136319/140‑1 (MQ=255)

                                          atCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGc                                                                                            <  2:892715/140‑1 (MQ=255)

                                           tCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGcc                                                                                           <  2:3891/140‑1 (MQ=255)

                                           tCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAACCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGcc                                                                                           <  2:1056583/140‑1 (MQ=255)

                                              cTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCcaca                                                                                        >  2:657959/1‑140 (MQ=255)

                                                gACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACAtt                                                                                      <  1:897839/140‑1 (MQ=255)

                                                 aCGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTc                                                                                     >  2:520701/1‑140 (MQ=255)

                                                   gTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACAtt                                                                                      <  1:581002/137‑1 (MQ=255)

                                                   gTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACAtt                                                                                      >  2:581002/1‑137 (MQ=255)

                                                      aTCCTTGAGGGGGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATCCGCTAt                                                                                >  2:447489/1‑140 (MQ=255)

                                                           tGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGcc                                                                           <  1:40964/140‑1 (MQ=255)

                                                           tGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGcc                                                                           <  1:802075/140‑1 (MQ=255)

                                                            gAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCt                                                                          >  2:568573/1‑140 (MQ=255)

                                                             aGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTg                                                                         >  2:682343/1‑140 (MQ=255)

                                                             aGGGTAAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATc                                                                               >  1:129158/1‑134 (MQ=255)

                                                             aGGGTAAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATc                                                                               <  2:129158/134‑1 (MQ=255)

                                                              gggTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTgc                                                                        <  1:1137466/140‑1 (MQ=255)

                                                                 tGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTgccgc                                                                     >  1:712316/1‑140 (MQ=255)

                                                                   aaGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATc                                                                               <  2:54019/128‑1 (MQ=255)

                                                                   aaGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATc                                                                               >  1:54019/1‑128 (MQ=255)

                                                                   aaGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCa                                                                   >  1:52837/1‑140 (MQ=255)

                                                                     gTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCaga                                                                 <  2:755081/140‑1 (MQ=255)

                                                                     gTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCaga                                                                 <  1:657959/140‑1 (MQ=255)

                                                                      ttCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCagag                                                                <  1:420322/140‑1 (MQ=255)

                                                                        cAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCAGAGCg                                                              >  1:674265/1‑140 (MQ=255)

                                                                          ggTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCAGAGCGtt                                                            <  1:602117/140‑1 (MQ=255)

                                                                               tcgAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCAGAGCGTTGCCAt                                                       >  1:540713/1‑140 (MQ=255)

                                                                               tcgAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCAGAGCGTTGCCAt                                                       >  2:865950/1‑140 (MQ=255)

                                                                               tcgAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCAGAGCGTTGCCAt                                                       >  2:744785/1‑140 (MQ=255)

                                                                                  aGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCAGAGCGTTGCCATCTg                                                    <  1:744785/140‑1 (MQ=255)

                                                                                  aGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATCCGCTATCGGCCTGCCGCCAGAGCGTTGCCATCTg                                                    <  1:447489/140‑1 (MQ=255)

                                                                                         cGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCAGAGCGTTGCCATCTGTTCCGTg                                             >  2:870030/1‑140 (MQ=255)

                                                                                             aaCGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCAGAGCGTTGCCATCTGTTCCGTGAGGa                                         <  1:520701/140‑1 (MQ=255)

                                                                                                 aaaCTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCAGAGCGTTGCCATCTGTTCCGTGAGGATGGc                                     >  1:642555/1‑140 (MQ=255)

                                                                                                  aaCTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCAGAGCGTTGCCATCTGTTCCGTGAGGATGGCa                                    <  1:181866/140‑1 (MQ=255)

                                                                                                   aCTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCAGAGCGTTGCCATCTGTTCCGTGAGGATGGCAc                                   >  1:567259/1‑140 (MQ=255)

                                                                                                    cTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCAGAGCGTTGCCATCTGTTCCGTGAGGATGGCACt                                  <  2:52837/140‑1 (MQ=255)

                                                                                                      cAAATCCATATCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCAGAGCGTTGCCATCTGTTCCGTGAGGATGGCACTGc                                <  1:999303/140‑1 (MQ=255)

                                                                                                             atatCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCAGAGCGTTGCCATCTGTTCCGTGAGGATGGCACTGCATgtcgt                         <  1:911174/140‑1 (MQ=255)

                                                                                                               atCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCAGAGCGTTGCCATCTGTTCCGTGAGGATGGCACTGCATGtcgtcg                       <  1:568573/140‑1 (MQ=255)

                                                                                                                tCCAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCAGAGCGTTGCCATCTGTTCCGTGAGGATGGCACTGCATGTCGTCGa                      <  2:938128/140‑1 (MQ=255)

                                                                                                                 ccAGATCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCAGAGCGTTGCCATCTGTTCCGTGAGGATGGCACTGCATGTCGTCGAc                     <  2:674265/140‑1 (MQ=255)

                                                                                                                     aTCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCAGAGCGTTGCCATCTGTTCCGTGAGGATGGCACTGCATgtcgtc                        >  1:698363/1‑133 (MQ=255)

                                                                                                                     aTCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCAGAGCGTTGCCATCTGTTCCGTGAGGATGGCACTGCATgtcgtc                        <  2:698363/133‑1 (MQ=255)

                                                                                                                     aTCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCAGAGCGTTGCCATCTGTTCCGTGAGGATGGCACTGCATGtcgtcg                       <  1:15956/134‑1 (MQ=255)

                                                                                                                     aTCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCAGAGCGTTGCCATCTGTTCCGTGAGGATGGCACTGCATGtcgtcg                       >  2:15956/1‑134 (MQ=255)

                                                                                                                     aTCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCAGAGCGTTGCCATCTGTTCCGTGAGGATGGCACTGCATGTCGTCGa                      <  1:907053/135‑1 (MQ=255)

                                                                                                                     aTCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCAGAGCGTTGCCATCTGTTCCGTGAGGATGGCACTGCATGTCGTCGa                      >  2:907053/1‑135 (MQ=255)

                                                                                                                     aTCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCAGAGCGTTGCCATCTGTTCCGTGAGGATGGCACTGCATGTCGTCGACTGCa                 >  1:742540/1‑140 (MQ=255)

                                                                                                                     aTCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCAGAGCGTTGCCATCTGTTCCGTGAGGATGGCACTGCATGTCGTCGACTGCa                 >  1:736456/1‑140 (MQ=255)

                                                                                                                     aTCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCAGAGCGTTGCCATCTGTTCCGTGAGGATGGCACTGCATGTCGTCGACTGCa                 >  2:831852/1‑140 (MQ=255)

                                                                                                                     aTCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCAGAGCGTTGCCATCTGTTCCGTGAGGATGGCACTGCATGTCGTCGACTGCa                 >  2:692327/1‑140 (MQ=255)

                                                                                                                     aTCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCAGAGCGTTGCCATCTGTTCCGTGAGGATGGCACTGCATGTCGTCGACTGCa                 >  2:705758/1‑140 (MQ=255)

                                                                                                                     aTCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCAGAGCGTTGCCATCTGTTCCGTGAGGATGGCACTGCATGTCGTCGACTGCa                 >  2:369800/1‑140 (MQ=255)

                                                                                                                     aTCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCAGAGCGTTGCCATCTGTTCCGTGAGGATGGCACTGCATGTCGTCGACTGCa                 >  1:449023/1‑140 (MQ=255)

                                                                                                                     aTCCCTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCAGAGCGTTGCCATCTATTCCGTGAGGATGGCACTGCATGTCGTCGACTGCa                 >  2:792052/1‑140 (MQ=255)

                                                                                                                        ccTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCAGAGCGTTGCCGTCTGTTCCGTGAGGATGGCACTGCATGTCGTCGACTGCATaa              >  2:760569/1‑140 (MQ=255)

                                                                                                                        ccTTCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCAGAGCGTTGCCATCTGTTCCGTGAGGATGGCACTGCATGTCGTCGACTGCATaa              >  1:310286/1‑140 (MQ=255)

                                                                                                                          ttCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACgtggt                                                                                                              >  2:1120549/1‑42 (MQ=255)

                                                                                                                          ttCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACgtggt                                                                                                              <  1:1120549/42‑1 (MQ=255)

                                                                                                                           tCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCAGAGCGTTGCCATCTGTTCCGTGAGGATGGCACTGCATgtcgtc                        <  1:524431/127‑1 (MQ=255)

                                                                                                                           tCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCAGAGCGTTGCCATCTGTTCCGTGAGGATGGCACTGCATgtcgtc                        >  2:524431/1‑127 (MQ=255)

                                                                                                                           tCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCAGAGCGTTGCCATCTGTTCCGTGAGGATGGCACTGCATGTCGTCGACTGCATAAGGa           >  1:684289/1‑140 (MQ=255)

                                                                                                                           tCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCAGAGCGTTGCCATCTGTTCCGTGAGGATGGCACTGCATGTCGTCGACTGCATAAGGa           >  2:12144/1‑140 (MQ=255)

                                                                                                                           tCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCAGAGCGTTGCCATCTGTTCCGTGAGGATGGCACTGCATGTCGTCGACTGCATAAGGa           >  1:341030/1‑140 (MQ=255)

                                                                                                                           tCCATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGCGCCACATTCGCTATCGGCCTGCCGCCAGAGCGTTGCCATCTGTTCCGTGAGGATGGCACTGCATGTCGTCGACTGCATAAGGa           >  1:618681/1‑140 (MQ=255)

                                                                                                                            ccATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCAGAGCGTTGCCATCTGTTCCGTGAGGATGGCACTGCATGTCGTCGACTGCATAAGGAg          >  2:330862/1‑140 (MQ=255)

                                                                                                                            ccATTCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCAGAGCGTTGCCATCTGTTCCGTGAGGATGGCACTGCATGTCGTCGACTGCATAAGGAg          >  1:775412/1‑140 (MQ=255)

                                                                                                                               ttCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCAGAGCGTTGCCATCTGTTCCGTGAGGATGGCACTGCATGTCGTCGACTGCATAAGGAGCCg       <  2:775412/140‑1 (MQ=255)

                                                                                                                               ttCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCAGAGCGTTGCCATCTGTTCCGTGAGGATGGCACTGCATGTCGTCGACTGCATAAGGAGCCg       >  1:259996/1‑140 (MQ=255)

                                                                                                                               ttCGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCAGAGCGTTGCCATCTGTTCCGTGAGGATGGCACTGCATGTCGTCGACTGCATAAGGAGCCg       <  2:540713/140‑1 (MQ=255)

                                                                                                                                 cGTCAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCAGAGCGTTGCCATCTGTTCCGTGAGGATGGCACTGCATGTCGTCGACTGCATAAGGAGCCggg     <  1:705758/140‑1 (MQ=255)

                                                                                                                                    cAAAGCCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCAGAGCGTTGCCATCTGTTCCGTGAGGATGGCACTGCATGTCGTCGACTGCATAAGGAGCCGGGCGt  >  1:984074/1‑140 (MQ=255)

ATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGTCAAAACCTGGTGTACCGCCAGAACGACGTGGTGTTGGTAGAAGAAGGTGCCACATTCGCTATCGGCCTGCCGCCAGAGCGTTGCCATCTGTTCCGTGAGGATGGCACTGCATGTCGTCGACTGCATAAGGAGCCGGGCGT  >  NC_000913/4247624‑4247895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`