Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,354,227	T→C	E164G (GAA→GGA)	lysU ←	lysine‑‑tRNA ligase/Ap4A synthetase/Ap3A synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,354,227	0	T	C	100.0%	262.4 / NA	75	E164G (GAA→GGA)	lysU	lysine‑‑tRNA ligase/Ap4A synthetase/Ap3A synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/41);  total (34/41)

GTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTCACCCGTTTGCGTCTTAAACAGCGTACCGCGGGCACCGATAATGTCAC  >  NC_000913/4354095‑4354362                                                                                                                                     |                                                                                                                                        gTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATc                                                                                                                                  <  2:818315/140‑1 (MQ=255)   ttCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCt                                                                                                                                <  1:323702/140‑1 (MQ=255)    tCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTg                                                                                                                               >  1:48899/1‑140 (MQ=255)     cTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGc                                                                                                                              <  2:613789/140‑1 (MQ=255)      tACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCa                                                                                                                             <  1:959260/140‑1 (MQ=255)      tACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCa                                                                                                                             <  2:397866/140‑1 (MQ=255)              tAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATgg                                                                                                                     >  1:254162/1‑140 (MQ=255)              tAAAGCCGCGCCCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGCCAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATgg                                                                                                                     >  2:854881/1‑140 (MQ=255)                aaGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGaa                                                                                                                   >  1:771736/1‑140 (MQ=255)                      cgcgcgACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGa                                                                                                                    <  2:41438/133‑1 (MQ=255)                      cgcgcgACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGa                                                                                                                    >  1:41438/1‑133 (MQ=255)                      cgcgcgACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATc                                                                                                             >  2:589819/1‑140 (MQ=255)                      cgcgcgACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATc                                                                                                             >  2:972100/1‑140 (MQ=255)                      cgcgcgACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATc                                                                                                             >  2:303330/1‑140 (MQ=255)                        cgcgACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTg                                                                                                           <  2:982519/140‑1 (MQ=255)                             ccATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTaaa                                                                                                      <  1:854881/140‑1 (MQ=255)                              cATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAg                                                                                                     <  1:530550/140‑1 (MQ=255)                              cATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAg                                                                                                     <  1:889066/140‑1 (MQ=255)                                tGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGa                                                                                                   <  2:771736/140‑1 (MQ=255)                                       aCGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTAt                                                                                                              >  2:590619/1‑122 (MQ=255)                                       aCGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTAt                                                                                                              <  1:590619/122‑1 (MQ=255)                                       aCGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAgcg                                                                                            >  1:840292/1‑138 (MQ=255)                                            tAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTa                                                                                       >  1:258230/1‑140 (MQ=255)                                                ggCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTca                                                                                   <  2:699337/140‑1 (MQ=255)                                                 gCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTcag                                                                                  >  1:128870/1‑140 (MQ=255)                                                  ccAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTcagc                                                                                 <  2:97337/140‑1 (MQ=255)                                                     gAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAgg                                                                              <  2:481179/140‑1 (MQ=255)                                                      aaTTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGgc                                                                             >  1:529234/1‑140 (MQ=255)                                                       aTTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGgcg                                                                            <  2:254162/140‑1 (MQ=255)                                                       aTTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGgcg                                                                            <  1:1083211/140‑1 (MQ=255)                                                          tttGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAg                                                                         <  1:589819/140‑1 (MQ=255)                                                           ttGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGc                                                                        <  2:529234/140‑1 (MQ=255)                                                            tGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCt                                                                       <  1:811380/140‑1 (MQ=255)                                                            tGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCt                                                                       <  1:1125345/140‑1 (MQ=255)                                                            tGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCt                                                                       <  2:756397/140‑1 (MQ=255)                                                              aaCGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCa                                                                     <  2:599232/140‑1 (MQ=255)                                                               aCGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAg                                                                    <  1:515003/140‑1 (MQ=255)                                                                cGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGt                                                                   <  1:962971/140‑1 (MQ=255)                                                                     aacaaACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGt                                                              <  1:893327/140‑1 (MQ=255)                                                                            gTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGa                                                       >  2:132041/1‑140 (MQ=255)                                                                            gTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGa                                                       <  1:303330/140‑1 (MQ=255)                                                                             tttGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCt                                                                       <  1:546487/123‑1 (MQ=255)                                                                             tttGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCt                                                                       >  2:546487/1‑123 (MQ=255)                                                                               tGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAg                                                    >  2:682149/1‑140 (MQ=255)                                                                               tGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAg                                                    >  2:26359/1‑140 (MQ=255)                                                                                  cGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTc                                                 <  2:774909/140‑1 (MQ=255)                                                                                          aTCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTCACCCGttt                                         >  2:583675/1‑140 (MQ=255)                                                                                          aTCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTCACCCGttt                                         <  1:972100/140‑1 (MQ=255)                                                                                          aTCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTCACCCGttt                                         <  2:356466/140‑1 (MQ=255)                                                                                                   gATGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTCACCCGTTTGCGTCTTaa                                <  2:102703/140‑1 (MQ=255)                                                                                                     tGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTCACCCGTTTGCGTCTTAAAc                              >  1:111324/1‑140 (MQ=255)                                                                                                     tGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTCACCCGTTTGCGTCTTAAAc                              >  2:575314/1‑140 (MQ=255)                                                                                                     tGAGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTCACCCGTTTGCGTCTTAAAc                              >  2:418591/1‑140 (MQ=255)                                                                                                       aGGTCCAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTCACCCGTTTGCGTCTTAAACAg                            <  1:583675/140‑1 (MQ=255)                                                                                                            cAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTCACCCGTTTGCGTCTTAAACAGCGTAc                       >  2:580157/1‑140 (MQ=255)                                                                                                            cAGATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTCACCCGTTTGCGTCTTAAACAGCGTAc                       >  1:839586/1‑140 (MQ=255)                                                                                                              gATAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTCACCCGTTTGCGTCTTAAACAGCGTACcg                     <  1:580157/140‑1 (MQ=255)                                                                                                                tAACGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTCACCCGTTTGCGTCTTAAACAGCGTACcgcg                   <  1:26359/140‑1 (MQ=255)                                                                                                                  aCGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTCACCCGTTTGCGTCTTAAACAGCGTACCGCggg                 <  1:575314/140‑1 (MQ=255)                                                                                                                   cGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTCACCCGTTTGCGTCTTAAACAGCGTACcg                     <  2:232664/135‑1 (MQ=255)                                                                                                                   cGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTCACCCGTTTGCGTCTTAAACAGCGTACcg                     >  1:232664/1‑135 (MQ=255)                                                                                                                   cGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTCACCCGTTTGCGTCTTAAACAGCGTACCGCGGGc                >  1:128828/1‑140 (MQ=255)                                                                                                                   cGTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTCACCCGTTTGCGTCTTAAACAGCGTACCGCGGGc                >  1:366716/1‑140 (MQ=255)                                                                                                                    gTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTCACCCGTTTGCGTCTTAAACAGCGTACCGCGGGCa               >  2:154691/1‑140 (MQ=255)                                                                                                                    gTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTCACCCGTTTGCGTCTTAAACAGCGTACCGCGGGCa               >  1:840194/1‑140 (MQ=255)                                                                                                                    gTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTCACCCGTTTGCGTCTTAAACAGCGTACCGCGGGCa               >  1:738135/1‑140 (MQ=255)                                                                                                                    gTTGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTCACCCGTTTGCGTCTTAAACAGCGTACCGCGGGCa               <  2:111324/140‑1 (MQ=255)                                                                                                                      tGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTCACCCGTTTGCGTCTTAAACAGCGTACCGCGGGCAcc             >  1:96220/1‑140 (MQ=255)                                                                                                                      tGACGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTCACCCGTTTGCGTCTTAAACAGCGTACCGCGGGCAcc             >  1:146884/1‑140 (MQ=255)                                                                                                                        aCGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTCACCCGTTTGCGTCTTAAACAGCGTACCGCGGGCACCGa           <  1:418591/140‑1 (MQ=255)                                                                                                                        aCGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTCACCCGTTTGCGTCTTAAACAGCGTACCGCGGGCACCGa           <  2:146884/140‑1 (MQ=255)                                                                                                                         cGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTCACCCGTTTGCGTCTTAAACAGCGTACCGCGGGCACCGAt          >  2:842395/1‑140 (MQ=255)                                                                                                                         cGATAACGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTCACCCGTTTGCGTCTTAAACAGCGTACCGCGGGCACCGAt          >  2:782314/1‑140 (MQ=255)                                                                                                                              aCGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTCACCCGTTTGCGTCTTAAACAGCGTACCGCGGGCACCGATAATGt     <  2:96220/140‑1 (MQ=255)                                                                                                                              aCGGACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTCACCCGTTTGCGTCTTAAACAGCGTACCGCGGGCACCGATAATGt     >  2:33206/1‑140 (MQ=255)                                                                                                                                 gACTCCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTCACCCGTTTGCGTCTTAAACAGCGTACCGCGGGCACCGATAATGTCAc  <  1:33206/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                        GTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACGGATAGCGGCCAGAATTTTTGAACGGACAACAAACGTTTGACGGGATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTCACCCGTTTGCGTCTTAAACAGCGTACCGCGGGCACCGATAATGTCAC  >  NC_000913/4354095‑4354362

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑