Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	301,581	A→G	L103L (TTA→CTA)	paoB ←	aldehyde dehydrogenase, FAD‑binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	301,581	0	A	G	100.0%	139.4 / NA	41	L103L (TTA→CTA)	paoB	aldehyde dehydrogenase, FAD‑binding subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/20);  total (21/20)

CGGCTAAAGCCTTCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTGCGCCGATGCGCAGCCCACCCGCGTCGGTCACTT  >  NC_000913/301451‑301709                                                                                                                                   |                                                                                                                                 cGGCTAAAGCCTTCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAGCTGACCAGa                                                                                                                         <  2:469259/140‑1 (MQ=255)              cAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAGCTGACCAGACGCGCCAGCGAg                                                                                                             >  1:707497/1‑139 (MQ=255)              cAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAGCTGACCAGACGCGCCAGCGAg                                                                                                             <  2:707497/139‑1 (MQ=255)                    ccgcGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAGATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAGCTGACCAGACGCGCCAGCGAGCAGGgcg                                                                                                      <  1:363453/140‑1 (MQ=255)                    ccgcGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAGCTGACCAGACGCGCCAGCGAGCAGGgcg                                                                                                      <  2:773027/140‑1 (MQ=255)                    ccgcGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAGCTGACCAGACGCGCCAGCGAGCAGGgcg                                                                                                      <  2:52601/140‑1 (MQ=255)                    ccgcGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAGCTGACCAGACGCGCCAGCGAGCAGGgcg                                                                                                      >  2:72845/1‑140 (MQ=255)                    ccgcGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAGCTGACCAGACGCGCCAGCGAGCAGGgcg                                                                                                      >  2:718198/1‑140 (MQ=255)                             cGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAGCTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGt                                                                                             <  2:840685/140‑1 (MQ=255)                               cTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAGCTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTAc                                                                                           >  1:1081367/1‑140 (MQ=255)                                          gcgcTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAGCTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTa                                                                                            <  2:1075849/128‑1 (MQ=255)                                          gcgcTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAGCTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTa                                                                                            >  1:1075849/1‑128 (MQ=255)                                          gcgcTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAGCTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTACCGCGTAGTCac                                                                                >  2:1057089/1‑140 (MQ=255)                                          gcgcTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAGCTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTACCGCGTAATCac                                                                                >  2:92884/1‑140 (MQ=255)                                                 ttGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAGCTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTACCGCGTAATCACGacgcac                                                                         <  2:973956/140‑1 (MQ=255)                                                      gggCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAGCTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTACCGCGTAATCACGACGCACGCGCt                                                                    >  2:1049242/1‑140 (MQ=255)                                                           gATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAGCTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTACCGCGTAATCACGACGCACGCGCTCGTGa                                                               <  1:1107247/140‑1 (MQ=255)                                                           gATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAGCTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTACCGCGTAATCACGACGCACGCGCTCGTGa                                                               >  2:1051648/1‑140 (MQ=255)                                                            aTTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAGCTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAg                                                              >  1:514100/1‑140 (MQ=255)                                                                      tAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAGCTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTc                                                    >  1:562861/1‑140 (MQ=255)                                                                        aaaaTAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAGCTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCgg                                                  <  2:1081367/140‑1 (MQ=255)                                                                        aaaaTAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAGCTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCgg                                                  <  1:1049242/140‑1 (MQ=255)                                                                          aaTAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAGCTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGgtg                                                <  1:1051648/140‑1 (MQ=255)                                                                               gggCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAGCTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCg                                           >  1:156125/1‑140 (MQ=255)                                                                                ggCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAGCTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGt                                          >  2:618312/1‑140 (MQ=255)                                                                                  cAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAGCTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTAc                                        <  1:1057089/140‑1 (MQ=255)                                                                                  cAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAGCTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTAc                                        <  1:72845/140‑1 (MQ=255)                                                                                     cgcgTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAGCTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAg                                     >  2:1002880/1‑140 (MQ=255)                                                                                      gcgTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAGCTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGt                                    >  1:910914/1‑140 (MQ=255)                                                                                          gcgcTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAGCTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTgcgc                                >  2:981120/1‑140 (MQ=255)                                                                                                      aTTACCTGCGGTGGTTGCCTGATTACGTAGCTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTGCGCCGATGCGCAGcc                    >  1:14222/1‑140 (MQ=255)                                                                                                        tACCTGCGGTGGTTGCCTGATTACGTAGCTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTGCGCCGATGCGCAGCCCa                  >  1:951885/1‑140 (MQ=255)                                                                                                         aCCTGCGGTGGTTGCCTGATTACGTAGCTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTGCGCCGATGCGCAGCCCAc                 <  2:773336/140‑1 (MQ=255)                                                                                                            tGCGGTGGTTGCCTGATTACGTAGCTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTGCGCCGATGCGCAGCCCACCcg              <  2:951885/140‑1 (MQ=255)                                                                                                                  ggtTGCCTGATTACGTAGCTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTGCGCCGATGCGCAGCCCACCCGCGTCgg        <  1:92884/140‑1 (MQ=255)                                                                                                                   gtTGCCTGATTACGTAGCTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTGCGCTGATGCGCAGCCCACCCGCGTCGGt       <  1:423518/140‑1 (MQ=255)                                                                                                                   gtTGCCTGATTACGTAGCTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTGCGCCGATGCGCAGCCCACCCGCGTCGGt       <  1:981120/140‑1 (MQ=255)                                                                                                                   gtTGCCTGATTACGTAGCTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTGCGCCGATGCGCAGCCCACCCGCGTCGGt       >  2:23783/1‑140 (MQ=255)                                                                                                                    ttGCCTGATTACGTAGCTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTGCGCCGATGCGCAGCCCACCCGCGTCGGTc      >  2:652676/1‑140 (MQ=255)                                                                                                                        cTGATTACGTAGCTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTGCGCCGATGCGCAGCCCACCCGCGTCGGTCACtt  >  2:715409/1‑140 (MQ=255)                                                                                                                        cTGATTACGTAGCTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTGCGCCGATGCGCAGCCCACCCGCGTCGGTCACtt  <  2:14222/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                 CGGCTAAAGCCTTCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTGCGGTGGTTGCCTGATTACGTAACTGACCAGACGCGCCAGCGAGCAGGGCGCGGGAGAGTACCGCGTAATCACGACGCACGCGCTCGTGAGCCGCCAGGTCGGTGTTCCGTACCAGTGCGCCGATGCGCAGCCCACCCGCGTCGGTCACTT  >  NC_000913/301451‑301709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑