Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,558,205	A→G	S29P (TCC→CCC)	hypT ←	DNA‑binding transcriptional dual regulator HypT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,558,205	0	A	G	100.0%	176.9 / NA	51	S29P (TCC→CCC)	hypT	DNA‑binding transcriptional dual regulator HypT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/27);  total (24/27)

AAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGG  >  NC_000913/4558069‑4558338                                                                                                                                         |                                                                                                                                      aaGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGgaaa                                                                                                                                    >  1:670439/1‑140 (MQ=255)      tttCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGGAAAATTGc                                                                                                                               <  2:670439/140‑1 (MQ=255)              gTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGGAAAATTGCGGCAtttt                                                                                                                       >  2:480940/1‑140 (MQ=255)                   gagaGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGGAAAATTGCGGCATTTTTCCAg                                                                                                                  <  2:872357/140‑1 (MQ=255)                    agagTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCTCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGGAAAATTGCGGCATTTTTCCa                                                                                                                   >  2:794802/1‑138 (MQ=255)                    agagTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGGAAAATTGCGGCATTTTTCCa                                                                                                                   <  1:794802/138‑1 (MQ=255)                    agagTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGGAAAATTGCGGCATTTTTCCAgg                                                                                                                 >  1:77069/1‑140 (MQ=255)                    agagTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGGAAAATTGCGGCATTTTTCCAgg                                                                                                                 >  1:4797/1‑140 (MQ=255)                      agTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGGAAAATTGCGGCATTTTTCCAGGGt                                                                                                               <  1:80743/140‑1 (MQ=255)                      agTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGGAAAATTGCGGCATTTTTCCAGGGt                                                                                                               <  2:4797/140‑1 (MQ=255)                            aGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGGAAAATTGCGGCATTTTTCCAGGGTCAGaaa                                                                                                         <  2:81493/140‑1 (MQ=255)                              cggcgTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGATTCGAGACGTTGCGACTGACCGCCGCCTGGGGGAAATTGCGGCATTTTTCCAGGGTCCGAAAAt                                                                                                       >  2:510233/1‑140 (MQ=255)                                 cgTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGGAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCAt                                                                                                    >  1:510540/1‑140 (MQ=255)                                  gTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGGAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATa                                                                                                   <  1:510233/140‑1 (MQ=255)                                  gTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGGAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATa                                                                                                   <  2:77069/140‑1 (MQ=255)                                  gTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGGAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATa                                                                                                   <  1:775079/140‑1 (MQ=255)                                          gCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGGAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCAtt                                                                                           >  2:746485/1‑140 (MQ=255)                                           cGGTTAAACAATTCAGCACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGGAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCAttt                                                                                          <  2:510540/140‑1 (MQ=255)                                           cGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGGAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCAttt                                                                                          <  1:480940/140‑1 (MQ=255)                                                aaaCAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGGTGCGAGACGTTGCGACTGACTGCCGCCCGGGGGAAAATGCGGCATTTTTTCAGGGGCAGAAAAACATAAAACCATTTTGttt                                                                                     >  2:1128508/1‑140 (MQ=255)                                                        cAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGGAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCAtt                                                                                           >  1:452638/1‑126 (MQ=255)                                                        cAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGGAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCAtt                                                                                           <  2:452638/126‑1 (MQ=255)                                                          acacCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGGAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATg                                                                           <  2:814534/140‑1 (MQ=255)                                                           cacCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGGAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGc                                                                          >  1:153331/1‑140 (MQ=255)                                                             ccAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGGAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCaa                                                                        <  2:890324/140‑1 (MQ=255)                                                              cAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGGAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCaaa                                                                       >  2:233142/1‑140 (MQ=255)                                                                aTCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGGAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAAt                                                                     >  1:668696/1‑140 (MQ=255)                                                                aTCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGGAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAAt                                                                     >  1:228168/1‑140 (MQ=255)                                                                     cTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGGAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGcac                                                                <  1:233142/140‑1 (MQ=255)                                                                       gTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGGAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGcacca                                                              <  2:618107/140‑1 (MQ=255)                                                                        ttCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACCGACTGCCGCCTGGGGAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGcaccac                                                             >  1:615091/1‑140 (MQ=255)                                                                          ccAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGGAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAg                                                           <  1:1128508/140‑1 (MQ=255)                                                                            aGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGGAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTc                                                         >  2:911998/1‑140 (MQ=255)                                                                             gcgcACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGGAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCa                                                        >  1:924780/1‑140 (MQ=255)                                                                               gcACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGGAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATc                                                      >  1:1065040/1‑140 (MQ=255)                                                                                  cGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGGAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCa                                                    >  1:971451/1‑139 (MQ=255)                                                                                  cGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGGAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCa                                                    <  2:971451/139‑1 (MQ=255)                                                                                  cGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGGAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCAt                                                   >  1:332707/1‑140 (MQ=255)                                                                                  cGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGGAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCAt                                                   >  2:892735/1‑140 (MQ=255)                                                                                  cGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGGAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCAt                                                   >  1:1123546/1‑140 (MQ=255)                                                                                   ggATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGGAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCAtt                                                  <  2:973046/140‑1 (MQ=255)                                                                                       gcggcgGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGGAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGt                                              <  1:864141/140‑1 (MQ=255)                                                                                        cggcggCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGGAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCCTCCATTACGtt                                             >  1:1012858/1‑140 (MQ=255)                                                                                           cggcTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGGAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTa                                          >  2:655142/1‑140 (MQ=255)                                                                                                             gagaCGTTGCGACTGACTGCCGCCTGGGGAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTg                        <  2:332707/140‑1 (MQ=255)                                                                                                             gagaCGTTGCGACTGACTGCCGCCTGGGGAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTg                        <  2:1123546/140‑1 (MQ=255)                                                                                                                 cGTTGCGACTGACTGCCGCCTGGGGAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCggg                    <  2:1065040/140‑1 (MQ=255)                                                                                                                 cGTTGCGACTGACTGCCGCCTGGGGAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCggg                    <  1:659861/140‑1 (MQ=255)                                                                                                                 cGTTGCGACTGACTGCCGCCTGGGGAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCggg                    <  1:278921/140‑1 (MQ=255)                                                                                                                     gCGACTGACTGCCGCCTGGGGAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCAtt                <  2:228168/140‑1 (MQ=255)                                                                                                                      cGACTGACTGCCGCCTGGGGAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCt                          >  1:1066450/1‑129 (MQ=255)                                                                                                                      cGACTGACTGCCGCCTGGGGAAAATTGCGGCACTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCt                          <  2:1066450/129‑1 (MQ=255)                                                                                                                              tgCCGCCTGGGGAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCa       >  2:486900/1‑140 (MQ=255)                                                                                                                                   ccTGGGGAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCgg  >  1:279838/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      AAGATTTTCCCTTGTTCTGAGAGTTGCAGCGGCGTTACCTGGCGGTTAAACAATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGG  >  NC_000913/4558069‑4558338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑