Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	319,878	T→C	T42A (ACC→GCC)	rclA ←	putative pyridine nucleotide‑disulfide oxidoreductase RclA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	319,878	0	T	C	100.0%	160.0 / NA	47	T42A (ACC→GCC)	rclA	putative pyridine nucleotide‑disulfide oxidoreductase RclA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/19);  total (28/19)

CAAGATTATGAAAATTCTTATTACGTAAAAAATTAACCACTTCATTTTTACGCTGTATGGCACGGACAAAATCTGTGTGCTGCTGTGCGTCATGAACCAATGTTTTGGTTGGGATGCAGCCGATATTAATACAGGTCCCGCCATACATTGCATTTGATTGTTCGATGAGAGCCACTCGCCAACCTGCTTTTGCCAGCGTGACGGCTAATGTTTTTCCAGCCTTGCCAAAACCAATAATCACTGCCTGATATTTATTCATGATGATT  >  NC_000913/319743‑320008                                                                                                                                        |                                                                                                                                   aaaGATTATAAAAATTCTTATTACGAAAAAAATTAACCACTTAATTTTTACGCTGTATGGCACGAAAAAAATCTGTGTGCTGCTGTGCGTCATGAACCAATGTTTTGGTTGGGATGCAGCCGATATTAATACAGGCCCCg                                                                                                                                <  2:1075568/139‑1 (MQ=255)   aGATTATGAAAATTTTTATTACGAAAAAAATTAACCACTTCATTTTTACGCTGTATGGCACGGAAAAAATCTGTGTGCTGCTGTGCGTCATGAACCAATGTTTTGGTTGGGATGCAGCCGATATTAATACAGGCCCCGcc                                                                                                                              <  1:534417/140‑1 (MQ=255)      ttATGAAAATTCTTATTACGTAAAAAATTAACCACTTCATTTTTACGCTGTATGGCACGGACAAAATCTGTGTGCTGCTGTGCGTCATGAACCAATGTTTTGGTTGGGATGCAGCCGATATTAATACAGGCCCCGCCATa                                                                                                                           >  1:244497/1‑140 (MQ=255)         tGAAAATTCTTATTACGTAAAAAATTAACCACTTCATTTTTACGCTGTATGGCACGGACAAAATCTGTGTGCTGCTGTGCGTCATGAACCAATGTTTTGGTTGGGATGCAGCCGATATTAATACAGGCCCCGCCATACAt                                                                                                                        >  1:878443/1‑140 (MQ=255)          aaaaaTTTTTATTACGAAAAAAATTACCCACTTCATTTTTACGCTGTATGGCACGGAAAAAATCTGTGTGCTGCTGTGCGTCATGAACCAATGTTTTGGTGGGGATGCAGCCGATATTAATACAGGCCCCGCCATACAtt                                                                                                                       <  2:1000169/139‑1 (MQ=255)              aTTCTTATTACGTAAAAAATTAACCACTTCATTTTTACGCTGTATGGCACGGACAAAATCTGTGTGCTGCTGTGCGTCATGAACCAATGTTTTGGTTGGGATGCAGCCGATATTAATACAGGCCCCGCCATACATTGCAt                                                                                                                   >  1:478183/1‑140 (MQ=255)                      taCGTAAAAAATTAACCACTTCATTTTTACGCTGTATGGCACGGACAAAATCTGTGTGCTGCTGTGCGTCATAAACCAATGTTTTGGTGGGGATGCAGCCGATATTAATACAGGCCCCGCCATACATTGCATTTGATTGt                                                                                                           <  1:882344/140‑1 (MQ=255)                       aCGAAAAAAATTAACCACTTCTTTTTTACGCTGTATGGCACGGAAAAAATCTGTGTGCTGCTGTGCGTAATGAACCAATTTTTTGGTGGGGATGCAGCCGATATTAATACAGGCCCCGCCATACATTGCATTTGATTGtt                                                                                                          <  2:605865/140‑1 (MQ=255)                        cGTAAAAAATTAACCACTTCATTTTTACGCTGTATGGCACGGACAAAATCTGTGTGCTGCTGTGCGTCATGAACCAATGTTTTGGTTGGGATGCAGCCGATATTAATACAGGCCCCGCCATACATTGCATTTGATTGTTc                                                                                                         >  1:33529/1‑140 (MQ=255)                          tAAAAAATTAACCACTTCATTTTTACGCTGTATGGCACGGACAAAATCTGTGTGCTGCTGTGCGTCATGAACCAATGTTTTGGTTGGGATGCAGCCGATATTAATACAGGCCCCGCCATACATTGCATTTGATTGTTCGa                                                                                                       >  1:605865/1‑140 (MQ=255)                                    aCCACTTCATTTTTACGCTGTATGGCACGGACAAAATCTGTGTGCTGCTGTGCGTCATGAACCAATGTTTTGGTTGGGATGCAGCCGATATTAATACAGGCCCCGCCATACATTGCATTTGATTGTTCGATGAGAGCCAc                                                                                             >  1:365914/1‑140 (MQ=255)                                    aCCACTTCATTTTTACGCTGTATGGCACGGACAAAATCTGTGTGCTGCTGTGCGTCATGAACCAATGTTTTGGTTGGGATGCAGCCGATATTAATACAGGCCCCGCCATACATTGCATTTGATTGTTCGATGAGAGCCAc                                                                                             >  1:535446/1‑140 (MQ=255)                                            ttttttACGCTGTATGGCACGGACAAAATCTGTGTGCTGCTGTGCGTCATGACCCAATTTTTTGGTTGGGATGCAGCAGATATTAATACAGGCCCCGCCATACATTGCATTTGATTGTTCGATGAGAGCCACTCGCCAAc                                                                                     <  2:976006/139‑1 (MQ=255)                                            ttttttACGCTGTAGGGCACGGAAAAAATCTGTGTGCTGCTGTGCGTCATGACCAAATGTTTTGGTGGGGATGCAGCCGATATTAATACAGGCCCCGCCATACATTGCATTTGATTGTTCGATGAGAGCCACTCGCCAAc                                                                                     <  2:478183/139‑1 (MQ=255)                                                ttACGCTGTATGGCACGGACAAAATCTGTGTGCTGCTGTGCGTCATGAACCAATGTTTTGGTTGGGATGCAGCCGATATTAATACAGGCCCCGCCATACATTGCATTTGATTGTTCGATGAGAGCCACTCGCCAACCTGc                                                                                 >  1:88188/1‑140 (MQ=255)                                                        tATGGCACGGACAAAATCTGTGTGCTGCTGTGCGTCATGAACCAATGTTTTGGTTGGGATGCAGCCGATATTAATACAGGCCCCGCCATACATTGCATTTGATTGTTCGATGAGAGCCACTCGCCAACCTGCTTTTGCCa                                                                         >  1:46816/1‑140 (MQ=255)                                                                ggACAAAATCTGTGTGCTGCTGTGCGTCATGAACCAATGTTTTGGTTGGGATGCAGCCGATATTAATACAGGCCCCGCCATACATTGCATTTGATTGTTCGATGAGAGCCACTCGCCAACCTGCTTTTGCCAGCGTGACg                                                                 >  1:1073577/1‑140 (MQ=255)                                                                ggACAAAATCTGTGTGCTGCTGTGCGTCATGAACCAATGTTTTGGTTGGGATGCAGCCGATATTAATACAGGCCCCGCCATACATTGCATTTGATTGTTCGATGAGAGCCACTCGCCAACCTGCTTTTGCCAGCGTGACg                                                                 >  1:1080973/1‑140 (MQ=255)                                                                  aCAAAATCTGTGTGCTGCTGTGCGTCATGAACCAATGTTTTGGTTGGGATGCAGCCGATATTAATACAGGCCCCGCCATACATTGCATTTGATTGTTCGATGAGAGCCACTCGCCAACCTGCTTTTGCCAGCGTGACGGc                                                               >  2:609238/1‑140 (MQ=255)                                                                  aCAAAATCTGTGTGCTGCTGTGCGTCATGAACCAATGTTTTGGTTGGGATGCAGCCGATATTAATACAGGCCCCGCCATACATTGCATTTGATTGTTCGATGAGAGCCACTCGCCAACCTGCTTTTGCCAGCGTGACGGc                                                               >  1:559506/1‑140 (MQ=255)                                                                  aCAAAATCTGTGTGCTGCTGTGCGTCATGAACCAATGTTTTGGTTGGGATGCAGCCGATATTAATACAGGCCCCGCCATACATTGCATTTGATTGTTCGATGAGAGCCACTCGCCAACCTGCTTTTGCCAGCGTGACGGc                                                               >  1:888464/1‑140 (MQ=255)                                                                  aCAAAATCTGTGTGCTGCTGTGCGTCATGAACCAATGTTTTGGTTGGGATGCAGCCGATATTAATACAGGCCCCGCCATACATTGCATTTGATTGTTCGATGAGAGCCACTCGCCAACCTGCTTTTGCCAGCGTGACGGc                                                               >  1:399777/1‑140 (MQ=255)                                                                      aaTCTGTGTGCTGCTGTGCGTCATGAACCAATGTTTTGGTTGGGATGCAGCCGATATTAATACAGGCCCCGCCATACATTGCATTTGATTGTTCGATGAGAGCCACTCGCCAACCTGCTTTTGCCAGCGTGACGGCTAAt                                                           <  1:990919/140‑1 (MQ=255)                                                                            tgtgCTGCTGTGCGTCATGAACCAATGTTTTGGTTGGGATGCAGCCGATATTAATACAGGCCCCGCCATACATTGCATTTGATTGTTCGATGAGAGCCACTCGCCAACCTGCTTTTGCCAGCGTGACGGCTAATGttttt                                                     >  2:622954/1‑140 (MQ=255)                                                                             gtgCTGCTGTGCGTCATGAACCAATGTTTTGGTTGGGATGCAGCCGATATTAATACAGGCCCCGCCATACATTGCATTTGATTGTTCGATGAGAGCCACTCGCCAACCTGCTTTTGCCAGCGTGACGGCTAATGTTTTTc                                                    <  2:399777/140‑1 (MQ=255)                                                                                ctgctgTGCGTCATGAACCAATGTTTTGGTTGGGATGCAGCCGATATTAATACAGGCCCCGCCATACATTGCATTTGATTGTTCGATGAGAGCCACTCGCCAACCTGCTTTTGCCAGCGTGACGGCTAATGTTTTTCCAg                                                 >  1:719624/1‑140 (MQ=255)                                                                                ctgctgTGCGTCATGAACCAATGTTTTGGTTGGGATGCAGCCGATATTAATACAGGCCCCGCCATACATTGCATTTGATTGTTCGATGAGAGCCACTCGCCAACCTGCTTTTGCCAGCGTGACGGCTAATGTTTTTCCAg                                                 >  1:272767/1‑140 (MQ=255)                                                                                 tgttgtgCGTCATGACCAATTGTTTTGGTGGGGATGCAGCCGATATAAATACAGCCCCCGCCATACATTGCATTTGATTGTTCGATGAGAGCCACTCGCCAACCTGCTTTTGCCAGCGTGACGGCTAATGTTTTTCCAGc                                                <  2:1080973/137‑1 (MQ=255)                                                                                  gctgTGCGTCATGAACCAATGTTTTGGTTGGGATGCAGCCGATATTAATACAGGCCCCGCCATACATTGCATTTGATTGTTCGATGAGAGCCACTCGCCAACCTGCTTTTGCCAGCGTGACGGCTAATGTTTTTCCAGcc                                               >  2:525505/1‑140 (MQ=255)                                                                                     gtgCGTCATAAACCAATGTTTTGGTGGGGATGCAGCCGATATTAATACAGGCCCCGCCATACATTGCATTTGATTGTTCGATGAGAGCCACTCGCCAACCTGCTTTTGCCAGCGTGACGGCTAATGTTTTTCCAGCCTTg                                            <  2:114072/140‑1 (MQ=255)                                                                                       gCGTCATGAACCAATGTTTTGGTTGGGATGCAGCCGATATTAATACAGGCCCCGCCATACATTGCATTTGATTGTTCGATGAGAGCCACTCGCCAACCTGCTTTTGCCAGCGTGACGGCTAATGTTTTTCCAGCCTTGcc                                          <  2:719624/140‑1 (MQ=255)                                                                                        cGTCATGAACCAATGTTTTGGTTGGGATGCAGCCGATATTAATACAGGCCCCGCCATACATTGCATTTGATTGTTCGATGAGAGCCACTCGCCAACCTGCTTTTGCCAGCGTGACGGCTAATGTTTTTCCAGCCTTGCCa                                         >  2:324161/1‑140 (MQ=255)                                                                                             tGAACCAATGTTTTGGTTGGGATGCAGCCGATATTAATACAGGCCCCGCCATACATTGCATTTGATTGTTCGATGAGAGCCACTCGCCAACCTGCTTTTGCCAGCGTGACGGCTAATGTTTTTCCAGc                                                >  2:254786/1‑128 (MQ=255)                                                                                             tGAACCAATGTTTTGGTTGGGATGCAGCCGATATTAATACAGGCCCCGCCATACATTGCATTTGATTGTTCGATGAGAGCCACTCGCCAACCTGCTTTTGCCAGCGTGACGGCTAATGTTTTTCCAGc                                                <  1:254786/128‑1 (MQ=255)                                                                                               aaCCAATGTTTTGGTTGGGATGCAGCCGATATTAATACAGGCCCCGCCATACATTGCATTTGATTGTTCGATGAGAGCCACTCGCCAACCTGCTTTTGCCAGCGTGACGGCTAATGTTTTTCCAGCCTTGCCAAAACCaa                                  <  1:893049/140‑1 (MQ=255)                                                                                               aaCCAATGTTTTGGTTGGGATGCAGCCGATATTAATACAGGCCCCGCCATACATTGCATTTGATTGTTCGATGAGAGCCACTCGCCAACCTGCTTTTGCCAGCGTGACGGCTAATGTTTTTCCAGCCTTGCCAAAACCaa                                  <  1:324161/140‑1 (MQ=255)                                                                                                    aTGTTTTGGTTGGGATGCAGCCGATATTAATACAGGCCCCGCCATACATTGCATTTGATTGTTCGATGAGAGCCACTCGCCAACCTGCTTTTGCCAGCGTGACGGCTAATGTTTTTCCAGCCTTGCCAAAACCAATAATc                             >  1:966262/1‑140 (MQ=255)                                                                                                     ttttttGGTGGGGATGCAGCCAATATTAATACAGCCCCCGCCATACATTGCATTTGATTGTTCGATGAGAGCCACTCGCCAACCTGCTTTTGCCAGCGTGACGGCTAATGTTTTTCCAGCCTTGCCAAAACCAATAATCa                            <  2:365914/138‑1 (MQ=255)                                                                                                     ttttttGGTGGGGAGGCACCGAATATTAATACAGCCCCCGCCATACATTGCATTTGATTGTTCGATGAGAGCCACTCGCCAACCTGCTTTTGCCAGCGTGACGGCTAATGTTTTTCCAGCCTTGCCAAAACCAATAATCa                            <  2:535446/138‑1 (MQ=255)                                                                                                     tGTTTTGGTTGGGATGCAGCCGATATTAATACAGGCCCCGCCATACATTGCATTTGATTGTTCGATGAGAGCCACTCGCCAACCTGCTTTTGCCAGCGTGACGGCTAATGTTTTTCCAGCCTTGCCAAAACCAATAATCa                            <  1:609238/140‑1 (MQ=255)                                                                                                         ttggttggGATGCAGCCGATATTAATACAGGCCCCGCCATACATTGCATTTGATTGTTCGATGAGAGCCACTCGCCAACCTGCTTTTGCCAGCGTGACGGCTAATGTTTTTCCAGCCTTGCCAAAACCAATAATCACTGc                        >  2:236925/1‑140 (MQ=255)                                                                                                           ggttggGATGCAGCCGATATTAATACAGGCCCCGCCATACATTGCATTTGATTGTTCGATGAGAGCCACTCGCCAACCTGCTTTTGCCAGCGTGACGGCTAATGTTTTTCCAGCCTTGCCAAAACCAATAATCACTGCCt                      <  2:966262/140‑1 (MQ=255)                                                                                                           ggttggGATGCAGCCGATATAAATCCAGCCCCCGCCATACATTGCATTTGATTGTTCGATGAGAGCCACTCGCCAACCTGCTTTTGCCAGCGTGACGGCTAATGTTTTTCCAGCCTTGCCAAAACCAATAATCACTGCCt                      <  2:761389/140‑1 (MQ=255)                                                                                                           ggtgggGATGCGGCCGATATAAATACAGCCCCCGCCATACATTGCATTTGATTGTTCGATGAGAGCCACTCGCCAACCTGCTTTTGCCAGCGTGACGGCTAATGTTTTTCCAGCCTTGCCAAAACCAATAATCACTGCCt                      <  2:272767/140‑1 (MQ=255)                                                                                                               gggATGCAGCCGATATTAATACGGGCCCCGCCATACATTGCATTTGATTGTTCGATGAGAGCCACTCGCCAACCTGCTTTTGCCAGCGTGACGGCTAATGTTTTTCCAGCCTTGCCAAAACCAATAATCACTGCCTGata                  <  1:918218/140‑1 (MQ=255)                                                                                                               gggATGCAGCCGATATTAATACAGGCCCCGCCATACATTGCATTTGATTGTTCGATGAGAGCCACTCGCCAACCTGCTTTTGCCAGCGTGACGGCTAATGTTTTTCCAGCCTTGCCAAAACCAATAATCACTGCCTGata                  >  2:528089/1‑140 (MQ=255)                                                                                                               gggATGCAGCCGATATTAATACAGGCCCCGCCATACATTGCATTTGATTGTTCGATGAGAGCCACTCGCCAACCTGCTTTTGCCAGCGTGACGGCTAATGTTTTTCCAGCCTTGCCAAAACCAATAATCACTGCCTGata                  >  1:679320/1‑140 (MQ=255)                                                                                                               gggATGCAGCCGATATTAATACAGGCCCCGCCATACATTGCATTTGATTGTTCGATGAGAGCCACTCGCCAACCTGCTTTTGCCAGCGTGACGGCTAATGTTTTTCCAGCCTTGCCAAAACCAATAATCACTGCCTGata                  <  1:525505/140‑1 (MQ=255)                                                                                                                ggATGCAGCCGATATTAATACAGGCCCCGCCATACATTGCATTTGATTGTTCGATGAGAGCCACTCGCCAACCTGCTTTTGCCAGCGTGACGGCTAATGTTTTTCCAGCCTTGCCAAAACCAATAATCACTGCCt                      <  2:972057/135‑1 (MQ=255)                                                                                                                ggATGCAGCCGATATTAATACAGGCCCCGCCATACATTGCATTTGATTGTTCGATGAGAGCCACTCGCCAACCTGCTTTTGCCAGCGTGACGGCTAATGTTTTTCCAGCCTTGCCAAAACCAATAATCACTGCCt                      >  1:972057/1‑135 (MQ=255)                                                                                                                ggATGCAGCCGATATTAATACAGGCCCCGCCATACATTGCATTTGATTGTTCGATGAGAGCCACTCGCCAACCTGCTTTTGCCAGCGTGACGGCTAATGTTTTTCCAGCCTTGCCAAAACCAATAATCACTGCCTGatat                 >  1:761389/1‑140 (MQ=255)                                                                                                                     cAGCCAATATTAATCCAGCCCCCGCCATACATTGCATTTGATTGTTCGATGAGAGCCACTCGCCAACCTGCTTTTGCCAGCGTGACGGCTAATGTTTTTCCAGCCTTGCCAAAACCAATAATCACTGCCTGAtatttatt            <  2:679320/140‑1 (MQ=255)                                                                                                                          aatatTAATCCAGGCCCCGCCATACATTGCATTTGATTGTTCGATGAGAGCCACTCGCCAACCTGCTTTTGCCAGCGTGACGGCTAATGTTTTTCCAGCCTTGCCAAAACCAATAATCACTGCCTGATATTTATTCatga       <  2:878443/139‑1 (MQ=255)                                                                                                                           atatTAATACAGGCCCCGCCATACATTGCATTTGATTGTTCGATGAGAGCCACTCGCCAACCTGCTTTTGCCAGCGTGACGGCTAATGTTTTTCCAGCCTTGCCAAAACCAATAATCACTGCCTGATATTTATTCatgat      >  2:17935/1‑140 (MQ=255)                                                                                                                               tAATACAGGCCCCGCCATACATTGCATTTGATTGTTCGATGAGAGCCACTCGCCAACCTGCTTTTGCCAGCGTGACGGCTAATGTTTTTCCAGCCTTGCCAAAACCAATAATCACTGCCTGATATTTATTCATGATGAtt  <  2:985899/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                   CAAGATTATGAAAATTCTTATTACGTAAAAAATTAACCACTTCATTTTTACGCTGTATGGCACGGACAAAATCTGTGTGCTGCTGTGCGTCATGAACCAATGTTTTGGTTGGGATGCAGCCGATATTAATACAGGTCCCGCCATACATTGCATTTGATTGTTCGATGAGAGCCACTCGCCAACCTGCTTTTGCCAGCGTGACGGCTAATGTTTTTCCAGCCTTGCCAAAACCAATAATCACTGCCTGATATTTATTCATGATGATT  >  NC_000913/319743‑320008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑