Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	37,682	T→C	N48S (AAC→AGC)	caiC ←	carnitine‑CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	37,682	0	T	C	100.0%	212.7 / NA	61	N48S (AAC→AGC)	caiC	carnitine‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/31);  total (30/31)

GTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTCGAGATGTAGTGCAACCTTGTCGCCTTTGCGAATCCCCAGCGTATAAAACAGGTTTGCCGTGCGGTTAATCTCCTGATTTAACTCAAGATAACTATACCGGTTAACGACTCCGCCGCTGGATTCACAAATCAGCGCCGTTTTATGACCGTAAACGTCCGCAAGATCGTCCCACATTTGACGTAGATGTTGTCCGCCAAT  >  NC_000913/37546‑37815                                                                                                                                         |                                                                                                                                      gTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTCGAGATGTAGTGCAACCTTGTCGCCTTTGCGAATCCCCAGCGTATAAAACAGGTTTGCCGTGCGGCTaa                                                                                                                                    <  2:867160/140‑1 (MQ=255)          cACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTCGAGATGTAGTGCAACCTTGTCGCCTTTGCGAATCCCCAGCGTATAAAACAGGTTTGCCGTGCGGCTAATCTCCTGAt                                                                                                                           >  2:142583/1‑140 (MQ=255)          cACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTCGAGATGTAGTGCAACCTTGTCGCCTTTGCGAATCCCCAGCGTATAAAACAGGTTTGCCGTGCGGCTAATCTCCTGAt                                                                                                                           >  1:1042574/1‑140 (MQ=255)              aTAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTCGAGATGTAGTGCAACCTTGTCGCCTTTGCGAATCCCCAGCGTATAAAACAGGTTTGCCGTGCGGCTAATCTCCTGATTTaa                                                                                                                       <  1:41935/140‑1 (MQ=255)               tAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTCGAGATGTAGTGCAACCTTGTCGCCTTTGCGAATCCCCAGCGTATAAAACAGGTTTGCCGTGCGGCTAAtctc                                                                                                                                >  2:222884/1‑130 (MQ=255)               tAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTCGAGATGTAGTGCAACCTTGTCGCCTTTGCGAATCCCCAGCGTATAAAACAGGTTTGCCGTGCGGCTAAtctc                                                                                                                                <  1:222884/130‑1 (MQ=255)               tAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTCGAGATGTAGTGCAACCTTGTCGCCTTTGCGAATCCCCAGCGTATAAAACAGGTTTGCCGTGCGGCTAATCTCCTGATTTAAc                                                                                                                      <  1:142583/140‑1 (MQ=255)                aaTCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTCGAGATGTAGTGCAACCTTGTCGCCTTTGCGAATCCCCAGCGTATAAAACAGGTTTGCCGTGCGGCTAATCTCCTGATTTAACt                                                                                                                     >  1:948531/1‑140 (MQ=255)                   cgcgCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTCGAGATGTAGTGCAACCTTGTCGCCTTTGCGAATCCCCAGCGTATAAAACAGGTTTGCCGTGCGGCTAATCTCCTGATTTAACTGaa                                                                                                                  <  2:264250/140‑1 (MQ=255)                    gcgcCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTCGAGATGTAGTGCAACCTTGTCGCCTTTGCGAATCCCCAGCGTATAAAACAGGTTTGCCGTGCGGCTAATCTCCTGAtt                                                                                                                          >  1:583164/1‑131 (MQ=255)                    gcgcCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTCGAGATGTAGTGCAACCTTGTCGCCTTTGCGAATCCCCAGCGTATAAAACAGGTTTGCCGTGCGGCTAATCTCCTGAtt                                                                                                                          <  2:583164/131‑1 (MQ=255)                    gcgcCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTCGAGATGTAGTGCAACCTTGTCGCCTTTGCGAATCCCCAGCGTATAAAACAGGTTTGCCGTGCGGCTAATCTCCTGATTTAACTCAAg                                                                                                                 >  2:380877/1‑140 (MQ=255)                     cgcCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTCGAGATGTAGTGCAACCTTGTCGCCTTTGCGAATCCCCAGCGTATAAAACAGGTTTGCCGTGCGGCTAATCTCCTGATTTAACTCAAGa                                                                                                                <  1:320162/140‑1 (MQ=255)                      gcCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTCGAGATGTAGTGCAACCTTGTCGCCTTTGCGAATCCCCAGCGTATAAAACAGGTTTGCCGTGCGGCTAATCTCCTGATTTAACTCAAGAt                                                                                                               <  1:380877/140‑1 (MQ=255)                              ttGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTCGAGATGTAGTGCAACCTTGTCGCCTTTGCGAATCCCCAGCGTATAAAACAGGTTTGCCGTGCGGCTAATCTCCTGATTTAACTCAAGATAACTATAc                                                                                                       <  2:1008179/140‑1 (MQ=255)                                gCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTCGAGATGTAGTGCAACCTTGTCGCCTTTGCGAATCCCCAGCGTATAAAACAGGTTTGCCGTGCGGCTAATCTCCTGATTTAACTCAAGATAACTATACCg                                                                                                     >  2:761237/1‑140 (MQ=255)                                                aaaaGATAAATTCCGGGCAGTTGTCGAGATGTAGTGCAACCTTGTCGCCTTTGCGAATCCCCAGCGTATAAAACAGGTTTGCCGTGCGGCTAATCTCCTGATTTAACTCAAGATAACTATACCGGTTAACGACTccgccg                                                                                     <  1:761237/140‑1 (MQ=255)                                                aaaaGATAAATTCCGGGCAGTTGTCGAGATGTAGTGCAACCTTGTCGCCTTTGCGAATCCCCAGCGTATAAAACAGGTTTGCCGTGCGGCTAATCCCCTGATTTAACTCAAGATAACTATACCGGTTAACGACTccgccg                                                                                     >  2:854531/1‑140 (MQ=255)                                                    gATAAATTCCGGGCAGTTGTCGAGATGTAGTGCAACCTTGTCGCCTTTGTGAATCCCCAGCGTATAAAACAGGTTTGCCGTGCGGCTAATCTCCTGATTTAACTCAAGATAACTATACCGGTTAACGACTCCGCCGCTgg                                                                                 <  1:260825/140‑1 (MQ=255)                                                               ggCAGTTGTCGAGATGTAGTGCAACCTTGTCGCCTTTGCGAATCCCCAGCGTATAAAACAGGTTTGCCGTGCGGCTAATCTCCTGATTTAACTCAAGATAACTATACCGGTTAACGACTCCGCCGCTGGATTCACAAATc                                                                      >  1:459574/1‑140 (MQ=255)                                                                    ttGTCGAGATGTAGTGCAACCTTGTCGCCTTTGCGAATCCCCAGCGTATAAAACAGGTTTGCCGTGCGGCTAATCTCCTGATTTAACTCAAGATAACTATACCGGTTAACGACTCCGCCGCTGGATTCACAAATCAgcgc                                                                 <  2:343248/140‑1 (MQ=255)                                                                              gTAGTGCAACCTTGTCGCCTTTGCGAATCCCCAGCGTATAAAACAGGTTTGCCGTGCGGCTAATCTCCTGATTTAACTCAAGATAACTATACCGGTTAACGACTCCGCCGCTGGATTCACAAATCAGCGCCGTTTTATGa                                                       >  2:604324/1‑140 (MQ=255)                                                                                 gTGCAACCTTGTCGCCTTTGCGAATCCCCAGCGTATAAAACAGGTTTGCCGTGCGGCTAATCTCCTGATTTAACTCAAGATAACTATACCGGTTAACGACTCCGCCGCTGGATTCACAAATCAGCGCCGTTTTATGACCg                                                    >  2:1061646/1‑140 (MQ=255)                                                                                    cAACCTTGTCGCCTTTGCGAATCCCCAGCGTATAAAACAGGTTTGCCGTGCGGCTAATCTCCTGATTTAACTCAAGATAACTATACCGGTTAACGACTCCGCCGCTGGATTCACAAATCAGCGCCGTTTTATGACCGTaa                                                 >  1:88072/1‑140 (MQ=255)                                                                                     aaCCTTGTCGCCTTTGCGAATCCCTAGCGTATAAAACAGGTTTGCCGTGCGGCTAATCTCCTGATTTAACTCAAGATAACTATACCGGTTAACGACTCCGCCGCTGGATTCACAAATCAGCGCCGTTTTATGACCGTaaa                                                >  1:912098/1‑140 (MQ=255)                                                                                     aaCCTTGTCGCCTTTGCGAATCCCCAGCGTATAAAACAGGTTTGCCGTGCGGCTAATCTCCTGATTTAACTCGAGATAACTATACCGGTTAACGACTCCGCCGCTGGATTCACAAATCAGCGCCGTTTTATGACCGTaaa                                                >  2:253897/1‑140 (MQ=255)                                                                                      aCCTTGTCGCCTTTGCGAATCCCCAGCGTATAAAACAGGTTTGCCGTGCGGCTAATCTCCTGATTTAACTCAAGATAACTATACCGGTTAACGACTCCGCCGCTGGATTCACAAATCAGCGCCGTTTTATGACCGTAAAc                                               >  1:798600/1‑140 (MQ=255)                                                                                      aCCTTGTCGCCTTTGCGAATCCCCAGCGTATAAAACAGGTTTGCCGTGCGGCTAATCTCCTGATTTAACTCAAGATAACTATACCGGTTAACGACTCCGCCGCTGGATTCACAAATCAGCGCCGTTTTATGACCGTAAAc                                               <  1:604324/140‑1 (MQ=255)                                                                                      aCCTTGTCGCCTTTGCGAATCCCCAGCGTATAAAACAGGTTTGCCGTGCGGCTAATCTCCTGATTTAACTCAAGATAACTATACCGGTTAACGACTCCGCCGCTGGATTCACAAATCAGCGCCGTTTTATGACCGTAAAc                                               <  2:88072/140‑1 (MQ=255)                                                                                      aCCTTGTCGCCTTTGCGAATCCCCAGCGTATAAAACAGGTTTGCCGTGCGGCTAATCTCCTGATTTAACTCAAGATAACTATACCGGTTAACGACTCCGCCGCTGGATTCACAAATCAGCGCCGTTTTATGACCGTAAAc                                               <  2:1042574/140‑1 (MQ=255)                                                                                        cTTGTCGCCTTTGCGAATCCCCAGCGTATAAAACAGGTTTGCCGTGCGGCTAATCTCCTGATTTAACTCAAGATAACTATACCGGTTAACGACTCCGCTGCTGGATTCACAAATCAGCGCCGTTTTATGACCGTAAACGt                                             >  2:200851/1‑140 (MQ=255)                                                                                           gTCGCCTTTGCGAATCCCCAGCGTATAAAACAGGTTTGCCGTGCGGCTAATCTCCTGATTTAACTCAAGATAACTATACCGGTTAACGACTCCGCCGCTGGATTCACAAATCAGCGCCGTTTTATGACCGTAAACg                                              >  2:713376/1‑136 (MQ=255)                                                                                           gTCGCCTTTGCGAATCCCCAGCGTATAAAACAGGTTTGCCGTGCGGCTAATCTCCTGATTTAACTCAAGATAACTATACCGGTTAACGACTCCGCCGCTGGATTCACAAATCAGCGCCGTTTTATGACCGTAAACg                                              <  1:713376/136‑1 (MQ=255)                                                                                                cTTTGCGAATCCCCAGCGTATAAAACAGGTTTGCCGTGCGGCTAATCTCCTGATTTAACTCAAGATAACTATACCGGTTAACGACTCCGCCGCTGGATTCACAAATCAGCGCCGTTTTATGACCGTAAACGTCCGCAAGa                                     <  2:882248/140‑1 (MQ=255)                                                                                                          ccccAGCGTATAAAACAGGTTTGCCGTGCGGCTAATCTCCTGATTTAACTCAAGATAACTATACCGGTTAACGACTCCGCCGCTGGATTCACAAATCAGCGCCGTTTTATGACCGTAAACGTCCGCAAGATCGTCCcaca                           >  1:539778/1‑140 (MQ=255)                                                                                                             cAGCGTATAAAACAGGTTTGCCGTGCGGCTAATCTCCTGATTTAACTCAAGATAACTATACCGGTTAACGACTCCGCCGCTGGATTCACAAATCAGCGCCGTTTTATGACCGTAAACGTCCGCAAGATCGTCCCACAttt                        >  1:575795/1‑140 (MQ=255)                                                                                                              aGCGTATAAAACAGGTTTGCCGTGCGGCTAATCTCCTGATTTAACTCAAGATAACTATACCGGTTAACGACTCCGCCGCTGGATTCACAAATCAGCGCCGTTTTATGACCGTAAACGTCCGCAAGATCGTCCCACATTTg                       >  2:357729/1‑140 (MQ=255)                                                                                                               gCGTATAAAACAGGTTTGCCGTGCGGCTAATCCCCTGATTTAACTCAAGATAACTATACCGGTTAACGACTCCGCCGCTGGATTCACAAATCAGCGCCGTTTTATGACCGTAAACGTCCGCAAGATCGTCCCACATTTGa                      <  1:854531/140‑1 (MQ=255)                                                                                                                cGTATAAAACAGGTTTGCCGTGCGGCTAATCTCCTGATTTAACTCAAGATAACTATACCGGTTAACGACTCCGCCGCTGGATTCACAAATCAGCGCCGTTTTATGACCGTAAACGTCCGCAAGATCGTCCCACATTTGAc                     <  1:1061646/140‑1 (MQ=255)                                                                                                                     aaaaCAGGTTTGCCGTGCGGCTAATCTCCTGATTTAACTCAAGATAACTATACCGGTTAACGACTCCGCCGCTGGATTCACAAATCAGCGCCGTTTTATGACCGTAAACGTCCGCAAGATCGTCCcaca                           >  2:384763/1‑129 (MQ=255)                                                                                                                     aaaaCAGGTTTGCCGTGCGGCTAATCTCCTGATTTAACTCAAGATAACTATACCGGTTAACGACTCCGCCGCTGGATTCACAAATCAGCGCCGTTTTATGACCGTAAACGTCCGCAAGATCGTCCcaca                           <  1:384763/129‑1 (MQ=255)                                                                                                                          aGGTTTGCCGTGCGGCTAATCTCCTGATTTAACTCAAGATAACTATACCTGTTAACGACTCCGCCGCTGGATTCACAAATCAGCGCCGTTTTATGACCGTAAACGTCCGCAAGATCGTCCCACATTTGACGTAGAtgttg           >  2:592660/1‑140 (MQ=255)                                                                                                                          aGGTTTGCCGTGCGGCTAATCTCCTGATTTAACTCAAGATAACTATACCGGTTAACGACTCCGCCGCTGGATTCACAAATCAGCGCCGTTTTATGACCGTAAACGTCCGCAAGATCGTCCCACATTTGACGTAGAtgttg           >  1:862280/1‑140 (MQ=255)                                                                                                                          aGGTTTGCCGTGCGGCTAATCTCCTGATTTAACTCAAGATAACTATACCGGTTAACGACTCCGCCGCTGGATTCACAAATCAGCGCCGTTTTATGACCGTAAACGTCCGCAAGATCGTCCCACATTTGACGTAGAtgttg           >  1:64880/1‑140 (MQ=255)                                                                                                                            gTTTGCCGTGCGGCTAATCTCCTGATTTAACTCAAGATAACTATACCGGTTAACGACTCCGCCGCTGGATTCACAAATCAGCGCCGTTTTATGACCGTAAACGTCCGCAAGATCGTCCCACATTTTACGTAGATGTTGTc         >  1:96538/1‑140 (MQ=255)                                                                                                                            gTTTGCCGTGCGGCTAATCTCCTGATTTAACTCAAGATAACTATACCGGTTAACGACTCCGCCGCTGGATTCACAAATCAGCGCCGTTTTATGACCGTAAACGTCCGCAAGATCGTCCCACATTTGACGTAGAtgttgt          >  2:177515/1‑139 (MQ=255)                                                                                                                            gTTTGCCGTGCGGCTAATCTCCTGATTTAACTCAAGATAACTATACCGGTTAACGACTCCGCCGCTGGATTCACAAATCAGCGCCGTTTTATGACCGTAAACGTCCGCAAGATCGTCCCACATTTGACGTAGAtgttgt          <  1:177515/139‑1 (MQ=255)                                                                                                                            gTTTGCCGTGCGGCTAATCTCCTGATTTAACTCAAGATAACTATACCGGTTAACGACTCCGCCGCTGGATTCACAAATCAGCGCCGTTTTATGACCGTAAACGTCCGCAAGATCGTCCCACATTTGACGTAGATGTTGTc         >  1:890052/1‑140 (MQ=255)                                                                                                                            gTTTGCCGTGCGGCTAATCTCCTGATTTAACTCAAGATAACTATACCGGTTAACGACTCCGCCGCTGGATTCACAAATCAGCGCCGTTTTATGACCGTAAACGTCCGCAAGATCGTCCCACATTTGACGTAGATGTTGTc         <  2:948531/140‑1 (MQ=255)                                                                                                                              ttGCCGTGCGGCTAATCTCCTGATTTAACTCAAGATAACTATACCTGTTAACGACTCCGCCGCTGGATTCACAAATCAGCGCCGTTTTATGACCGTAAACGTCCGCAAGATCGTCCCACATTTGACGTAGATGTTGTCCg       <  1:592660/140‑1 (MQ=255)                                                                                                                              ttGCCGTGCGGCTAATCTCCTGATTTAACTCAAGATAACTATACCGGTTAACGACTCCGCCGCTGGATTCACAAATCAGCGCCGTTTTATGACCGTAAACGTCCGCAAGATCGTCCCACATTTGACGTAGAtgttg           >  2:205200/1‑136 (MQ=255)                                                                                                                              ttGCCGTGCGGCTAATCTCCTGATTTAACTCAAGATAACTATACCGGTTAACGACTCCGCCGCTGGATTCACAAATCAGCGCCGTTTTATGACCGTAAACGTCCGCAAGATCGTCCCACATTTGACGTAGAtgttg           <  1:205200/136‑1 (MQ=255)                                                                                                                               tGCCGTGCGGCTAATCTCCTGATTTAACTCAAGATAACTATACCGGTTAACGACTCCGCCGCTGGATTCACAAATCAGCGCCGTTTTATGACCGTAAACGTCCGCAAGATCGTCCCACATTTGACg                    >  2:988551/1‑126 (MQ=255)                                                                                                                               tGCCGTGCGGCTAATCTCCTGATTTAACTCAAGATAACTATACCGGTTAACGACTCCGCCGCTGGATTCACAAATCAGCGCCGTTTTATGACCGTAAACGTCCGCAAGATCGTCCCACATTTGACg                    <  1:988551/126‑1 (MQ=255)                                                                                                                               tGCCGTGCGGCTAATCTCCTGATTTAACTCAAGATAACTATACCGGTTAACGACTCCGCCGCTGGATTCACAAATCAGCGCCGTTTTATGACCGTAAACGTCCGCAAGATCGTCCCACATTTGACGTAGATGTTGTCCGc      <  2:539778/140‑1 (MQ=255)                                                                                                                               tGCCGTGCGGCTAATCTCCTGATTTAACTCAAGATAACTATACCGGTTAACGACTCCGCCGCTGGATTCACAAATCAGCGCCGTTTTATGACCGTAAACGTCCGCAAGATCGTCCCACATTTGACGTAGATGTTGTCCGc      <  2:862280/140‑1 (MQ=255)                                                                                                                               tGCCGTGCGGCTAATCTCCTGATTTAACTCAAGATAACTATACCGGTTAACGACTCCGCCGCTGGATTCACAAATCAGCGCCGTTTTATGACCGTAAACGTCCGCAAGATCGTCCCACATTTGACGTAGATGTTGTCCGc      <  2:231954/140‑1 (MQ=255)                                                                                                                               tGCCGTGCGGCTAATCTCCTGATTTAACTCAAGATAACTATACCGGTTAACGACTCCGCCGCTGGATTCACAAATCAGCGCCGTTTTATGACCGTAAACGTCCGCAAGATCGTCCCACATTTGACGTAGATGTTGTCCGc      <  1:357729/140‑1 (MQ=255)                                                                                                                                gCCGTGCGGCTAATCTCCTGATTTAACTCAAGATAACTATACCGGTTAACGACTCCGCCGCTGGATTCACAAATCAGCGCCGTTTTATGACCGTAAACGTCCGCAAGATCGTCCCACATTTGACGTAGAtgttg           <  2:609087/134‑1 (MQ=255)                                                                                                                                gCCGTGCGGCTAATCTCCTGATTTAACTCAAGATAACTATACCGGTTAACGACTCCGCCGCTGGATTCACAAATCAGCGCCGTTTTATGACCGTAAACGTCCGCAAGATCGTCCCACATTTGACGTAGAtgttg           >  1:609087/1‑134 (MQ=255)                                                                                                                                   gTGCGGCTAATCTCCTGATTTAACTCAAGATAACTATACCGGTTAACGACTCCGCCGCTGGATTCACAAATCAGCGCCGTTTTATGACCGTAAACGTCCTCAAGATCGTCCCACATTTGACGTAGATGTTGTCCGCCAAt  <  2:494353/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      GTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTCGAGATGTAGTGCAACCTTGTCGCCTTTGCGAATCCCCAGCGTATAAAACAGGTTTGCCGTGCGGTTAATCTCCTGATTTAACTCAAGATAACTATACCGGTTAACGACTCCGCCGCTGGATTCACAAATCAGCGCCGTTTTATGACCGTAAACGTCCGCAAGATCGTCCCACATTTGACGTAGATGTTGTCCGCCAAT  >  NC_000913/37546‑37815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑