Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	429,137	G→A	L497L (TTG→TTA)	secD →	Sec translocon accessory complex subunit SecD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	429,137	0	G	A	100.0%	269.3 / NA	77	L497L (TTG→TTA)	secD	Sec translocon accessory complex subunit SecD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/41);  total (36/41)

AGGGGCTGGAGGCTTGCCTTGCCGGTCTGCTGGTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAAT  >  NC_000913/429001‑429271                                                                                                                                         |                                                                                                                                       aGGGGCTGGAGGCTTGCCTTGCCGGTCTGCTGGTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCa                                                                                                                                     >  1:119848/1‑140 (MQ=255)     gCTGGAGGCTTGCCTTGCCGGTCTGCTGGTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGgcg                                                                                                                                 >  1:656359/1‑140 (MQ=255)     gCTGGAGGCTTGCCTTGCCGGTCTGCTGGTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGgcg                                                                                                                                 >  2:402137/1‑140 (MQ=255)     gCTGGAGGCTTGCCTTGCCGGTCTGCTGGTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGgcg                                                                                                                                 <  2:650903/140‑1 (MQ=255)      cTGGAGGCTTGCCTTGCCGGTCTGCTGGTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGgcgc                                                                                                                                <  1:662657/140‑1 (MQ=255)         gAGGCTTGCCTTGCCGGTCTGCTGGTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAAc                                                                                                                             <  1:36121/140‑1 (MQ=255)          gggCTTGCCTTGCCGGTCTGCTGGTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACg                                                                                                                            <  2:680290/139‑1 (MQ=255)          aGGCTTGCCTTGCCGGTCTGCTGGTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACg                                                                                                                            <  2:652626/140‑1 (MQ=255)          aGGCTTGCCTTGCCGGTCTGCTGGTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACg                                                                                                                            <  2:1014709/140‑1 (MQ=255)          aGGCTTGCCTTGCCGGTCTGCTGGTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACg                                                                                                                            <  1:338097/140‑1 (MQ=255)          aGGCTTGCCTTGCCGGTCTGCTGGTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACg                                                                                                                            <  1:795325/140‑1 (MQ=255)            gCTTGCCTTGCCGGTCTGCTGGTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCt                                                                                                                          <  1:191752/140‑1 (MQ=255)             cttgccttgcCGGTCTGCTGGTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTg                                                                                                                         <  1:912969/140‑1 (MQ=255)               tgccttgccGGTCTGCTGGTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAg                                                                                                                       >  1:1120898/1‑140 (MQ=255)                  cttgccGGTCTGCTGGTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTAt                                                                                                                    <  1:777867/140‑1 (MQ=255)                         gTCTGCTGGTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGt                                                                                                             <  2:656359/140‑1 (MQ=255)                          tCTGCTGGTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACg                                                                                                                            >  1:764225/1‑124 (MQ=255)                          tCTGCTGGTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACg                                                                                                                            <  2:764225/124‑1 (MQ=255)                              ctgGTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATcgc                                                                                                        >  1:1050292/1‑140 (MQ=255)                               tgGTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATcgcg                                                                                                       >  2:306178/1‑140 (MQ=255)                                ggTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCgg                                                                                                      <  2:359594/140‑1 (MQ=255)                                  tttCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGt                                                                                                    <  1:232749/140‑1 (MQ=255)                                   ttCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTa                                                                                                   <  1:877961/140‑1 (MQ=255)                                   ttCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTa                                                                                                   <  2:941718/140‑1 (MQ=255)                                   ttCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTa                                                                                                   <  2:162668/140‑1 (MQ=255)                                      tATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCACGCGCAACGCTGAGCATGCCAGGTATCGCGGGTATCg                                                                                                >  2:532806/1‑140 (MQ=255)                                       aTTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGt                                                                                               >  2:982234/1‑140 (MQ=255)                                          cTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCtt                                                                                            <  1:839754/140‑1 (MQ=255)                                          cTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCtt                                                                                            <  1:982234/140‑1 (MQ=255)                                           tGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTa                                                                                           >  2:500698/1‑140 (MQ=255)                                             ttCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAAc                                                                                         >  1:721461/1‑140 (MQ=255)                                             ttCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAAc                                                                                         >  2:559742/1‑140 (MQ=255)                                             ttCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAAc                                                                                         >  1:214957/1‑140 (MQ=255)                                                 tGATCATCTTCTAT‑AGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTg                                                                                    >  2:69494/1‑140 (MQ=255)                                                            tataAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGt                                                                          <  1:246372/140‑1 (MQ=255)                                                               aagaagTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCCGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGa                                                                       <  1:749293/140‑1 (MQ=255)                                                               aagaagTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGa                                                                       <  1:1071388/140‑1 (MQ=255)                                                                agaagTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTgg                                                                              <  1:364622/132‑1 (MQ=255)                                                                agaagTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTgg                                                                              >  2:364622/1‑132 (MQ=255)                                                                   agTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCg                                                                   <  2:214957/140‑1 (MQ=255)                                                                     tttGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGAt                                                                      <  1:109347/135‑1 (MQ=255)                                                                     tttGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGAt                                                                      >  2:109347/1‑135 (MQ=255)                                                                     tttGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGaa                                                                 >  1:962279/1‑140 (MQ=255)                                                                     tttGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGaa                                                                 >  1:185631/1‑140 (MQ=255)                                                                     tttGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGaa                                                                 >  2:1131190/1‑140 (MQ=255)                                                                     tttGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGa                                                                  <  1:314877/139‑1 (MQ=255)                                                                     tttGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGa                                                                  >  2:314877/1‑139 (MQ=255)                                                                       tGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACg                                                               <  2:1050292/140‑1 (MQ=255)                                                                        ggTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGt                                                              <  2:185631/140‑1 (MQ=255)                                                                         gTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTa                                                             <  1:402137/140‑1 (MQ=255)                                                                          tCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTAc                                                            <  2:98785/140‑1 (MQ=255)                                                                          tCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTAc                                                            >  2:766045/1‑140 (MQ=255)                                                                              aTTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGAt                                                        >  2:1081810/1‑140 (MQ=255)                                                                               ttGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTa                                                             <  2:327173/134‑1 (MQ=255)                                                                               ttGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTa                                                             >  1:327173/1‑134 (MQ=255)                                                                                    aCCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCaacga                                                  <  2:721461/140‑1 (MQ=255)                                                                                    aCCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCaacga                                                  >  1:384242/1‑140 (MQ=255)                                                                                     ccAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGCGGCGGTCGATGCGAACGTACTGATCaacgaa                                                 <  1:697222/140‑1 (MQ=255)                                                                                        gTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGt                                              >  2:1004198/1‑140 (MQ=255)                                                                                        gTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGt                                              >  2:601833/1‑140 (MQ=255)                                                                                         tGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTa                                             <  2:119848/140‑1 (MQ=255)                                                                                          gCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTAt                                            <  1:601833/140‑1 (MQ=255)                                                                                            tctGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTAt                                            >  1:646739/1‑138 (MQ=255)                                                                                            tctGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTAt                                            <  2:646739/138‑1 (MQ=255)                                                                                             ctGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCaacgaa                                                 >  2:30024/1‑132 (MQ=255)                                                                                             ctGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCaacgaa                                                 <  1:30024/132‑1 (MQ=255)                                                                                               gATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTAGATGCGAACGTACTGATCAACGAACGTATTAaag                                       >  1:407795/1‑140 (MQ=255)                                                                                                        cTTGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAAACCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGa                              >  2:776865/1‑140 (MQ=255)                                                                                                          tGATCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGc                            <  1:776865/140‑1 (MQ=255)                                                                                                            aTCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCaa                          >  1:130837/1‑140 (MQ=255)                                                                                                            aTCTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCaa                          <  1:624306/140‑1 (MQ=255)                                                                                                              cTTAATCGTCGGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACg                        >  1:584063/1‑140 (MQ=255)                                                                                                                     gtcgGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTAc                 <  1:306178/140‑1 (MQ=255)                                                                                                                      tcgGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACt                >  1:991292/1‑140 (MQ=255)                                                                                                                       cgGCATTATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTg               >  1:796998/1‑140 (MQ=255)                                                                                                                             tATGTCGCTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAAc         >  1:712495/1‑140 (MQ=255)                                                                                                                                    cTGTTACCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAAt  >  2:817709/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       AGGGGCTGGAGGCTTGCCTTGCCGGTCTGCTGGTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAAT  >  NC_000913/429001‑429271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑