Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	844,562	T→G	T307P (ACC→CCC)	ybiO ←	moderate conductance mechanosensitive channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	844,562	0	T	G	100.0%	182.3 / NA	52	T307P (ACC→CCC)	ybiO	moderate conductance mechanosensitive channel
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (24/28);  total (24/28)

GCCAGCCAGTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGTCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTATCTGTACATTCACCTGATTAGAGATAATCGGCACGGCCACAATCAGGCCATAACCTATCAAACTGCTTAACCAGCTCAGGCGACGACTCC  >  NC_000913/844429‑844690                                                                                                                                      |                                                                                                                                 gccagccaGTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGGCATGca                                                                                                                            <  1:81856/140‑1 (MQ=255) gccagccaGTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGGCATGca                                                                                                                            <  2:713545/140‑1 (MQ=255)          tGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGGCATGCACAGCATAat                                                                                                                   >  2:717390/1‑140 (MQ=255)            ccACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGGCATGCACAGCATAatga                                                                                                                 <  1:230932/140‑1 (MQ=255)            ccACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGGCATGCACAGCATAatga                                                                                                                 <  2:278263/140‑1 (MQ=255)             cacaCCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGGCATGCACAGCATAatgat                                                                                                                >  2:7706/1‑140 (MQ=255)                acCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGGCATGCACAGCATAATGATGAc                                                                                                             >  2:11697/1‑140 (MQ=255)                    gcgcAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGGCATGCACAGCATAATGATGACGTTc                                                                                                         >  1:181513/1‑140 (MQ=255)                       cAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGGCATGCACAGCATAATGATGACGTTCGcc                                                                                                      >  2:746450/1‑140 (MQ=255)                       cAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGGCATGCACAGCATAATGATGACGTTCGcc                                                                                                      >  1:891974/1‑140 (MQ=255)                           ggCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGGCATGCACAGCATAATGATGACGTTCGCCAgcg                                                                                                  <  1:1146738/140‑1 (MQ=255)                             cgcgGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGGCATGCACAGCATAATGATGACGTTCGCCAGCGCa                                                                                                <  2:181513/140‑1 (MQ=255)                             cgcgGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGGCATGCACAGCATAATGATGACGTTCGCCAGCGCa                                                                                                <  1:739600/140‑1 (MQ=255)                             cgcgGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGGCATGCACAGCATAATGATGACGTTCGCCAGCGCa                                                                                                <  1:773239/140‑1 (MQ=255)                              gcgGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGGCATGCACAGCATAATGATGACGTTCGCCAGCGCAc                                                                                               <  2:683604/140‑1 (MQ=255)                                   tAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGGCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTATc                                                                                          <  2:891974/140‑1 (MQ=255)                                          gCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGGCATGCACAGCATAATGATGACGTTCGCCAGCGCAc                                                                                               >  1:922010/1‑128 (MQ=255)                                          gCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGGCATGCACAGCATAATGATGACGTTCGCCAGCGCAc                                                                                               <  2:922010/128‑1 (MQ=255)                                           cTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGGCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTATCt                                                                                         <  2:455747/133‑1 (MQ=255)                                           cTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGGCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTATCt                                                                                         >  1:455747/1‑133 (MQ=255)                                            tGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGGCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTATc                                                                                          <  2:298345/131‑1 (MQ=255)                                            tGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGGCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTATc                                                                                          >  1:298345/1‑131 (MQ=255)                                              aaaaaGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGGCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTATCTGTACATTCAc                                                                               <  1:7706/140‑1 (MQ=255)                                               aaaaGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGGCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTATCTGTACATTCAcc                                                                              <  1:11697/140‑1 (MQ=255)                                                    gCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGGCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTATCTGTAc                                                                                     <  1:1071454/128‑1 (MQ=255)                                                    gCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGGCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTATCTGTAc                                                                                     >  2:1071454/1‑128 (MQ=255)                                                    gCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGGCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTATCTGTACa                                                                                    <  1:936062/129‑1 (MQ=255)                                                    gCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGGCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTATCTGTACa                                                                                    >  2:936062/1‑129 (MQ=255)                                                        gCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGGCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTATCTGTACATTCACCTGATTagag                                                                     >  2:298312/1‑140 (MQ=255)                                                                  cgcgAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGGCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTATCTGTACATTCACCTGATTAGAGATAATCGGCa                                                           >  2:1050405/1‑140 (MQ=255)                                                                  cgcgAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGGCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTATCTGTACATTCACCTGATTAGAGATAATCGGCa                                                           <  1:298312/140‑1 (MQ=255)                                                                             gCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGGCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTATCTGTACATTCACCTGATTAGAGATAATCGGCACGGCCACAATc                                                <  2:414069/140‑1 (MQ=255)                                                                                   gCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGGCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTATCTGTACATTCACCTGATTAGGGATAATCGGCACGGCCACAATCAGGCCa                                          >  2:328358/1‑140 (MQ=255)                                                                                   gCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGGCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTATCTGTACATTCACCTGATTAGAGATAATCGGCACGGCCACAATc                                                >  1:39911/1‑134 (MQ=255)                                                                                   gCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGGCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTATCTGTACATTCACCTGATTAGAGATAATCGGCACGGCCACAATc                                                <  2:39911/134‑1 (MQ=255)                                                                                             ctctTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGGCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTATCTGTACATTCACCTGATTAGAGATAATCGGCACGGCCACAATCAGGCCATAACCTATCa                                <  1:980737/140‑1 (MQ=255)                                                                                             ctctTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGGCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTATCTGTACATTCACCTGATTAGAGATAATCGGCACGGCCACAATCAGGCCATAACCTATCa                                <  1:1126214/140‑1 (MQ=255)                                                                                                 tttttATTACGAAAGATCAGGTACAACGCCCAGACGGGCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTATCTGTACATTCACCTGATTAGAGATAATCGGCACGGCCACAATCAGGCCATAACCTATCAAACt                            >  2:182057/1‑140 (MQ=255)                                                                                                   tttATTACGAAAGATCAGGTACAACGCCCAGACGGGCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTATCTGTACATTCACCTGATTAGAGATAATCGGCACGGCCACAATCAGGCCATAACCTATCAAACTGc                          <  1:746450/140‑1 (MQ=255)                                                                                                       ttaCGAAAGATCAGGTACAACGCCCAGACGGGCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTATCTGTACATTCACCTGATTAGAGATAATCGGCACGGCCACAATCAGGCCATAACCTa                                   >  1:668614/1‑127 (MQ=255)                                                                                                       ttaCGAAAGATCAGGTACAACGCCCAGACGGGCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTATCTGTACATTCACCTGATTAGAGATAATCGGCACGGCCACAATCAGGCCATAACCTa                                   <  2:668614/127‑1 (MQ=255)                                                                                                                aTCAGGTACAACGCCCAGACGGGCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTATCTGTACATTCACCTGATTAGAGATAATCGGCACGGCCACAATCAGGCCATAACCTATCaa                               >  2:578809/1‑122 (MQ=255)                                                                                                                aTCAGGTACAACGCCCAGACGGGCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTATCTGTACATTCACCTGATTAGAGATAATCGGCACGGCCACAATCAGGCCATAACCTATCaa                               <  1:578809/122‑1 (MQ=255)                                                                                                                aTCAGGTACAACGCCCAGACGGGCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTATCTGTACATTCACCTGATTAGAGATAATCGGCACGGCCACAATCAGGCCATAACCTATCAAACTGCTTAACCAGCTCAg             >  2:257317/1‑140 (MQ=255)                                                                                                                    ggTACAACGCCCAGACGGGCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTATCTGTACATTCACCTGATTAGAGATAATCGGCACGGCCACAATCAGGCCATAACCTATCAAACTGCTTAACCAGCTCAGGcga         >  1:22625/1‑140 (MQ=255)                                                                                                                     gTACAACGCCCAGACGGGCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTAGCTGTACATTCACCTGATTAGAGATAATCGGCACGGCCACAATCAGGCCATAACCTATCAAACTGCTTAACCAGCTCAGGcgac        <  1:717390/140‑1 (MQ=255)                                                                                                                          aCGCCCAGACGGGCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTATCTGTACATTCACCTGATTAGAGATAATCGGCACGGCCACAATCAGGCCATAACCTATCAAACTGCTTAACCAGCTCAGGcgac        >  1:1150195/1‑135 (MQ=255)                                                                                                                          aCGCCCAGACGGGCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTATCTGTACATTCACCTGATTAGAGATAATCGGCACGGCCACAATCAGGCCATAACCTATCAAACTGCTTAACCAGCTCAGGcgac        <  2:1150195/135‑1 (MQ=255)                                                                                                                          aCGCCCAGACGGGCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTATCTGTACATTCACCTGATTAGAGATAATCGGCACGGCCACAATCAGGCCATAACCTATCAAACTGCTTAACCAGCTCAGGcgac        <  1:1113242/135‑1 (MQ=255)                                                                                                                          aCGCCCAGACGGGCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTATCTGTACATTCACCTGATTAGAGATAATCGGCACGGCCACAATCAGGCCATAACCTATCAAACTGCTTAACCAGCTCAGGcgac        >  2:1113242/1‑135 (MQ=255)                                                                                                                           cGCCCAGACGGGCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTATCTGTACATTCACCTGATTAGAGATAATCGGCACGGCCACAATCAGGCCATAACCTATCAAACTGCTTAACCAGCTCAGGCGACGACTcc  >  1:313425/1‑140 (MQ=255)                                                                                                                           cGCCCAGACGGGCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTATCTGTACATTCACCTGATTAGAGATAATCGGCACGGCCACAATCAGGCCATAACCTATCAAACTGCTTAACCAGCTCAGGCGACGACTcc  >  2:293790/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                 GCCAGCCAGTGCCACACCAGCGCAAAGGCGCGGATAAACAGGCTGAAAAAGGCCAGCGAATGCTCCGCGAAGTTGAGCAAATGCTGGGTAATCTCTTTTTTATTACGAAAGATCAGGTACAACGCCCAGACGGTCATGCACAGCATAATGATGACGTTCGCCAGCGCACCTATCTGTACATTCACCTGATTAGAGATAATCGGCACGGCCACAATCAGGCCATAACCTATCAAACTGCTTAACCAGCTCAGGCGACGACTCC  >  NC_000913/844429‑844690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑