Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 52,315 T→G Q39P (CAG→CCG)  rsmA ← 16S rRNA m(6)2A1518,m(6)2A1519 dimethyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091352,3150TG100.0% 338.6 / NA 97Q39P (CAG→CCG) rsmA16S rRNA m(6)2A1518,m(6)2A1519 dimethyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (45/52);  total (45/52)

GGCCTAAGAATGGATGCGTTTGCAGACGTGCCGCCAGATCGCGGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCTGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCCTGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTG  >  NC_000913/52184‑52451
                                                                                                                                   |                                                                                                                                        
ggCCTAAGAATGGATGCGTTTGCAGACGTGCCGCCAGATCGCGGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCttt                                                                                                                                  <  1:911171/140‑1 (MQ=255)
  ccTAAGAATGGATGCGTTTGCAGACGTGCCGCCAGATCGCGGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTg                                                                                                                                <  2:865741/140‑1 (MQ=255)
     aaGAATGGATGCGTTTGCAGACGTGCCGCCAGATCGCGGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCgg                                                                                                                             >  2:426632/1‑140 (MQ=255)
     aaGAATGGATGCGTTTGCAGACGTGCCGCCAGATCGCGGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCgg                                                                                                                             >  1:780519/1‑140 (MQ=255)
     aaGAATGGATGCGTTTGCAGACGTGCCGCCAGATCGCGGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCgg                                                                                                                             >  2:723718/1‑140 (MQ=255)
       gAATGGATGCGTTTGCAGACGTGCCGCCAGATCGCGGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGt                                                                                                                           <  1:1135138/140‑1 (MQ=255)
         aTGGATGCGTTTGCAGACGTGCCGCCAGATCGCGGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTa                                                                                                                         <  1:147778/140‑1 (MQ=255)
           ggATGCGTTTGCAGACGTGCCGCCAGATCGCGGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAAt                                                                                                                       >  1:824264/1‑140 (MQ=255)
           ggATGCGTTTGCAGACGTGCCGCCAGATCGCGGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAAt                                                                                                                       >  2:1046424/1‑140 (MQ=255)
           ggATGCGTTTGCAGACGTGCCGCCAGATCGCGGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAAt                                                                                                                       >  2:502672/1‑140 (MQ=255)
              tGCGTTTGCAGACGTGCCGCCAGATCGCGGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATggg                                                                                                                    <  1:1046424/140‑2 (MQ=255)
              tGCGTTTGCAGACGTGCCGCCAGATCGCGGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGc                                                                                                                    <  2:824264/140‑1 (MQ=255)
              tGCGTTTGCAGACGTGCCGCCAGATCGCGGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGc                                                                                                                    <  1:723718/140‑1 (MQ=255)
              tGCGTTTGCAGACGTGCCGCCAGATCGCGGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGc                                                                                                                    <  1:1110541/140‑1 (MQ=255)
                cGTTTGCAGACGTGCCGCCAGATCGCGGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAg                                                                                                                  >  2:677371/1‑140 (MQ=255)
                cGTTTGCAGACGTGCCGCCAGATCGCGGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAg                                                                                                                  >  2:103927/1‑140 (MQ=255)
                 gTTTGCAGACGTGCCGCCAGATCGCGGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGGCCTTTTGCGGGTTAATGGCAGa                                                                                                                 <  1:713136/140‑1 (MQ=255)
                 gTTTGCAGACGTGCCGCCAGATCGCGGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGTGGGTTAATGGCAGa                                                                                                                 <  1:239952/140‑1 (MQ=255)
                  tttGCAGACGTGCCGCCAGATCGCGGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGac                                                                                                                >  1:1133711/1‑140 (MQ=255)
                  tttGCAGACGTGCCGCCAGATCGCGGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGac                                                                                                                >  1:260474/1‑140 (MQ=255)
                  tttGCAGACGTGCCGCCAGATCGCGGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGa                                                                                                                 <  1:673627/139‑1 (MQ=255)
                  tttGCAGACGTGCCGCCAGATCGCGGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGa                                                                                                                 >  2:673627/1‑139 (MQ=255)
                   ttGCAGACGTGCCGCCAGATCGCGGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGaca                                                                                                               <  2:198910/140‑1 (MQ=255)
                     gCAGACGTGCCGCCAGATCGCGGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGAcaca                                                                                                             <  1:103927/140‑1 (MQ=255)
                      cAGACGTGCCGCCAGATCGCGGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGc                                                                                                                    <  2:124163/132‑1 (MQ=255)
                      cAGACGTGCCGCCAGATCGCGGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGc                                                                                                                    >  1:124163/1‑132 (MQ=255)
                      cAGACGTGCCGCCAGATCGCGGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCa                                                                                                                   <  1:1135367/133‑1 (MQ=255)
                      cAGACGTGCCGCCAGATCGCGGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCa                                                                                                                   >  2:1135367/1‑133 (MQ=255)
                      cAGACGTGCCGCCAGATCGCGGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGa                                                                                                                 >  2:951886/1‑135 (MQ=255)
                      cAGACGTGCCGCCAGATCGCGGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGa                                                                                                                 <  1:951886/135‑1 (MQ=255)
                         aCGTGCCGCCAGATCGCGGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGTCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATAc                                                                                                         >  2:1089763/1‑140 (MQ=255)
                          cGTGCCGCCAGATCGCGGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACt                                                                                                        >  2:614271/1‑140 (MQ=255)
                           gTGCCGCCAGATCGCGGTCAAGTTCGATGACCGTCAGCTGGTCCAGGCGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTg                                                                                                       >  2:101633/1‑140 (MQ=255)
                            tGCCGCCAGATCGCGGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTTGGTCAATGTCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGt                                                                                                      <  1:1089763/140‑1 (MQ=255)
                            tGCCGCCAGATCGCGGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGt                                                                                                      <  2:450907/140‑1 (MQ=255)
                            tGCCGCCAGATCGCGGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGt                                                                                                      <  1:614271/140‑1 (MQ=255)
                             gccgccAGATCGCGGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTc                                                                                                     >  2:1034332/1‑140 (MQ=255)
                              ccgccAGATCGCGGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGAcaca                                                                                                             <  2:502364/131‑1 (MQ=255)
                              ccgccAGATCGCGGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGAcaca                                                                                                             >  1:1139165/1‑131 (MQ=255)
                              ccgccAGATCGCGGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGAcaca                                                                                                             >  1:502364/1‑131 (MQ=255)
                              ccgccAGATCGCGGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGAcaca                                                                                                             <  2:1139165/131‑1 (MQ=255)
                                       cgcgGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAAc                                                                                           <  2:260474/140‑1 (MQ=255)
                                        gcgGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACt                                                                                          <  1:625456/140‑1 (MQ=255)
                                         cgGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACt                                                                                          >  1:995891/1‑139 (MQ=255)
                                         cgGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACt                                                                                          <  2:995891/139‑1 (MQ=255)
                                         cgGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTg                                                                                         >  1:915094/1‑140 (MQ=255)
                                         cgGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTg                                                                                         >  2:492986/1‑140 (MQ=255)
                                           gTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGAt                                                                                       >  2:960887/1‑140 (MQ=255)
                                             cAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCg                                                                                     <  1:502672/140‑1 (MQ=255)
                                               aGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGtt                                                                                   >  1:484614/1‑140 (MQ=255)
                                                gTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTg                                                                                  >  1:871920/1‑140 (MQ=255)
                                                 ttCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTga                                                                                 >  1:1138973/1‑140 (MQ=255)
                                                  tCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTgag                                                                                <  2:1133711/140‑1 (MQ=255)
                                                      tGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAg                                                                            >  1:348208/1‑140 (MQ=255)
                                                      tGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAg                                                                            >  1:346688/1‑140 (MQ=255)
                                                       gACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGt                                                                           <  1:243351/140‑1 (MQ=255)
                                                        aCCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGtt                                                                          >  1:131872/1‑140 (MQ=255)
                                                         ccGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGttt                                                                         >  2:663778/1‑140 (MQ=255)
                                                         ccGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGttt                                                                         <  2:484614/140‑1 (MQ=255)
                                                          cGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGtttt                                                                        >  1:586134/1‑140 (MQ=255)
                                                            tCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGc                                                                      >  2:51018/1‑140 (MQ=255)
                                                              aGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGccc                                                                    <  1:636310/140‑1 (MQ=255)
                                                                 tGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGaa                                                                 <  1:960887/140‑1 (MQ=255)
                                                                  ggTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAg                                                                >  1:468544/1‑140 (MQ=255)
                                                                      cAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGtt                                                            <  2:131872/140‑1 (MQ=255)
                                                                        gACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTa                                                          >  2:920511/1‑140 (MQ=255)
                                                                        gACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGCGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTa                                                          <  2:1116186/140‑1 (MQ=255)
                                                                             tCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGc                                                     >  1:404763/1‑140 (MQ=255)
                                                                              cGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCt                                                    >  2:820098/1‑140 (MQ=255)
                                                                              cGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCt                                                    >  2:443044/1‑140 (MQ=255)
                                                                                 cGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAg                                                 <  1:677371/140‑1 (MQ=255)
                                                                                 cGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAg                                                 <  1:260270/140‑1 (MQ=255)
                                                                                 cGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAg                                                 <  1:277930/140‑1 (MQ=255)
                                                                                  gACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGt                                                <  2:468544/140‑1 (MQ=255)
                                                                                    ccGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTgg                                              <  1:492986/140‑1 (MQ=255)
                                                                                     cGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGc                                             <  2:780519/140‑1 (MQ=255)
                                                                                           ggTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCCtggt                                       <  2:586134/140‑1 (MQ=255)
                                                                                              cAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCCtgg                                        >  2:302750/1‑136 (MQ=255)
                                                                                              cAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCCtgg                                        <  1:302750/136‑1 (MQ=255)
                                                                                              cAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCCTGGTGGa                                    <  1:51018/140‑1 (MQ=255)
                                                                                              cAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCCTGGTGGa                                    <  1:509534/140‑1 (MQ=255)
                                                                                                aTGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCCTGGTGGACt                                  <  1:443044/140‑1 (MQ=255)
                                                                                                 tGCCGCCAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCCTGGTGGACTc                                 >  1:282264/1‑140 (MQ=255)
                                                                                                      ccAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCCTGGTGGACTCGatta                            <  1:101633/140‑1 (MQ=255)
                                                                                                      ccAGACCGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCCTTTACGGGCTAAGTGGCCCTGGTGGACTCGatta                            <  2:1018927/140‑1 (MQ=255)
                                                                                                           ccGGGGCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCCTGGTGGACTCGATTATTCAt                       <  2:346688/140‑1 (MQ=255)
                                                                                                             ggggCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCCTGGTGGa                                    <  2:745024/125‑1 (MQ=255)
                                                                                                             ggggCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCCTGGTGGa                                    <  2:79994/125‑1 (MQ=255)
                                                                                                             ggggCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCCTGGTGGa                                    >  1:79994/1‑125 (MQ=255)
                                                                                                             ggggCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCCTGGTGGa                                    >  1:745024/1‑125 (MQ=255)
                                                                                                               ggCCGATTTCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCCTGGTGGACTCGATTATTCATTggg                   <  2:1138973/140‑1 (MQ=255)
                                                                                                                       tCGACCATCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCCTGGTGGACTCGATTATTCATTGGGTGTTAACa           <  2:611339/140‑1 (MQ=255)
                                                                                                                              tCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCCTGGTGGACTCGATTATTCATTGGGTGTTa              <  2:1138874/130‑1 (MQ=255)
                                                                                                                              tCGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCCTGGTGGACTCGATTATTCATTGGGTGTTa              >  1:1138874/1‑130 (MQ=255)
                                                                                                                               cGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCCTGGTGGACTCGATTATTCATTGGGTGTTAACAATCAtttt   >  1:493161/1‑140 (MQ=255)
                                                                                                                               cGCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCCTGGTGGACTCGATTATTCATTGGGTGTTAACAATCAtttt   >  2:445141/1‑140 (MQ=255)
                                                                                                                                gCCGGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACAAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCCTGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTg  <  1:45248/140‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                        
GGCCTAAGAATGGATGCGTTTGCAGACGTGCCGCCAGATCGCGGTCAAGTTCGATGACCGTCAGCTGGTCCAGACGTTCGCCGACCGGTTCGGTCAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCTGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCCTGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTG  >  NC_000913/52184‑52451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑