Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,180,225	A→G	T236A (ACC→GCC)	cobB →	protein‑lysine deacetylase/desuccinylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,180,225	0	A	G	100.0%	202.0 / NA	58	T236A (ACC→GCC)	cobB	protein‑lysine deacetylase/desuccinylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/27);  total (31/27)

CGAAATGCCACTCGGCATGGATGAAATTTATATGGCGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGATTAAAAGCGGGAAGCATTGCCTG  >  NC_000913/1180092‑1180358                                                                                                                                      |                                                                                                                                      cGAAATGCCACTCGGCATGGATGAAATTTATATGGCGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACGCCGTgg                                                                                                                                 >  1:840588/1‑140 (MQ=255)     aTGCCACTCGGCATGGATGAAATTTATATGGCGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACGCCGTGGAACt                                                                                                                             <  2:431458/140‑1 (MQ=255)           cTCGGCATGGATGAAATTTATATGGCGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCt                                                                                                                       <  1:706502/140‑1 (MQ=255)               gCATGGATGAAATTTATATGGCGTTGTCGATGTCCGATATCTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGACACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGaa                                                                                                                   <  2:1016185/140‑1 (MQ=255)               gCATGGATGAAATTTATATGGCGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGaa                                                                                                                   <  1:602385/140‑1 (MQ=255)                cATGGATGAAATTTATATGGCGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGAAc                                                                                                                  >  2:368192/1‑140 (MQ=255)                cATGGATGAAATTTATATGGCGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGAAc                                                                                                                  >  2:220334/1‑140 (MQ=255)                     aTGAAATTTATATGGCGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGAACCGAGt                                                                                                             <  1:865715/140‑1 (MQ=255)                        aaaTTTATATGGCGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGa                                                                                                                    >  2:475040/1‑130 (MQ=255)                        aaaTTTATATGGCGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGa                                                                                                                    <  1:475040/130‑1 (MQ=255)                          aTTTATATGGCGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGAACCGAGTCAGGt                                                                                                        >  2:412205/1‑140 (MQ=255)                              atatGGCGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGAACCGAGTCAGGt                                                                                                        >  1:215831/1‑136 (MQ=255)                              atatGGCGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGAACCGAGTCAGGt                                                                                                        <  2:215831/136‑1 (MQ=255)                                  ggCGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATg                                                                                                >  2:817432/1‑140 (MQ=255)                                    cGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGAACCGAGTCAGGt                                                                                                        >  2:989221/1‑130 (MQ=255)                                    cGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGAACCGAGTCAGGt                                                                                                        <  1:989221/130‑1 (MQ=255)                                      ttGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAAtt                                                                                            >  2:419928/1‑140 (MQ=255)                                       tGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAAttt                                                                                           >  2:250526/1‑140 (MQ=255)                                       tGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAAttt                                                                                           >  2:524096/1‑140 (MQ=255)                                        gTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTg                                                                                          >  2:835025/1‑140 (MQ=255)                                         tCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGc                                                                                         >  1:298694/1‑140 (MQ=255)                                         tCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGc                                                                                         <  2:812102/140‑1 (MQ=255)                                          cGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGcc                                                                                        <  1:524096/140‑1 (MQ=255)                                          cGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGcc                                                                                        <  2:364259/140‑1 (MQ=255)                                              ggCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCgaga                                                                                    >  2:386039/1‑140 (MQ=255)                                                        ttCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACgg                                                                          <  1:412205/140‑1 (MQ=255)                                                         tCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCg                                                                                       <  1:480201/126‑1 (MQ=255)                                                         tCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCg                                                                                       >  2:480201/1‑126 (MQ=255)                                                         tCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGc                                                                         >  1:490484/1‑140 (MQ=255)                                                             tGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCgg                                                                     <  1:220334/140‑1 (MQ=255)                                                              gCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGc                                                                    >  2:865352/1‑140 (MQ=255)                                                              gCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGc                                                                    <  1:399727/140‑1 (MQ=255)                                                                  ttGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGc                                                                <  1:340011/140‑1 (MQ=255)                                                                    ggCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCa                                                              >  1:731570/1‑140 (MQ=255)                                                                      cacaTCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAgg                                                            <  2:490484/140‑1 (MQ=255)                                                                        caTCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAggtg                                                          <  2:840588/140‑1 (MQ=255)                                                                        caTCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAggtg                                                          <  2:418364/140‑1 (MQ=255)                                                                              ggCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAGGTGGTGCCt                                                    >  2:40481/1‑140 (MQ=255)                                                                              ggCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAGGTGGTGCCt                                                    >  2:417342/1‑140 (MQ=255)                                                                                     ttATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAggtg                                                          <  2:942004/127‑1 (MQ=255)                                                                                     ttATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAggtg                                                          >  1:942004/1‑127 (MQ=255)                                                                                          cggcggCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGaa                                        <  1:496304/140‑1 (MQ=255)                                                                                             cggcTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCCGGGGGGGGCCGAGTTTGTTGaaaaa                                     >  2:820620/1‑139 (MQ=255)                                                                                             cggcTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAg                                     >  1:426232/1‑140 (MQ=255)                                                                                                   ggTTTGTTCACGAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTg                               <  1:229980/140‑1 (MQ=255)                                                                                                   ggTTTGTTCACGAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTg                               <  1:865352/140‑1 (MQ=255)                                                                                                         ttCACGAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAgggg                         >  1:758512/1‑139 (MQ=255)                                                                                                         ttCACGAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGa                         >  1:72168/1‑140 (MQ=255)                                                                                                              gAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGATTaaa                    >  2:1072143/1‑140 (MQ=255)                                                                                                              gAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGATTaaa                    >  2:510253/1‑140 (MQ=255)                                                                                                              gAAGCGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGATTaaa                    >  2:1024391/1‑140 (MQ=255)                                                                                                                  cGAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGATTAAAAGCg                >  1:23740/1‑140 (MQ=255)                                                                                                                   gAAACTGCATGGCGCGCACGCCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGATTAAAAGCgg               <  2:174017/140‑1 (MQ=255)                                                                                                                           aTGGCGCGCACGCCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGATTAAAAGCGGGAAGCAtt       <  1:835025/140‑1 (MQ=255)                                                                                                                           aTGGCGCGCACGCCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGATTAAAAGCGGGAAGCAtt       >  1:185897/1‑140 (MQ=255)                                                                                                                              gcgcgcACGCCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGATTAAAAGCGGGAAGCATTGcc    <  1:419928/140‑1 (MQ=255)                                                                                                                               cgcgcACGCCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAACCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGATTAAAAGCGGGAAGCATTGCCt   >  2:542188/1‑140 (MQ=255)                                                                                                                                gcgcACGCCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGATTAAAAGCGGGAAGCATTGCCTg  <  1:32723/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                      CGAAATGCCACTCGGCATGGATGAAATTTATATGGCGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGATTAAAAGCGGGAAGCATTGCCTG  >  NC_000913/1180092‑1180358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑