Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 77,011 C→T V97I (GTT→ATT)  sgrR ← DNA‑binding transcriptional dual regulator SgrR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091377,0110CT98.1% 175.3 / ‑4.4 52V97I (GTT→ATT) sgrRDNA‑binding transcriptional dual regulator SgrR
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (25/26);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

CGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACCAGTTGATCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCGCGCCCGACTTCCGCTTCC  >  NC_000913/76881‑77135
                                                                                                                                  |                                                                                                                            
cgcAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAATCAACTGCAc                                                                                                                     >  2:353463/1‑140 (MQ=255)
     tGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAATCAACTGCACCAGtt                                                                                                                <  2:598522/140‑1 (MQ=255)
       cgcTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAATCAACTGCACCAGTTGa                                                                                                              <  1:711182/140‑1 (MQ=255)
        gcTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAATCAACTGCACCAGTTGAt                                                                                                             <  1:353463/140‑1 (MQ=255)
          tGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAATCAACTGCACCAGTTGATCg                                                                                                           >  1:907778/1‑140 (MQ=255)
            ccAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAATCAACTGCACCAGTTGATCGAt                                                                                                         <  1:738325/140‑1 (MQ=255)
             cAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAATCAACTGCACCAGTTGATCGATa                                                                                                        <  1:405648/140‑1 (MQ=255)
                      gATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAATCAACTGCACCAGTTGATCGATACGATCCTGc                                                                                               >  2:108001/1‑140 (MQ=255)
                           cGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAATCAACTGCACCAGTTGATCGATACGATCCTGCTCcag                                                                                          <  2:907778/140‑1 (MQ=255)
                              aaCGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAATCAACTGCACCAGTTGATCGATACGATCCTGCTc                                                                                             <  2:949206/134‑1 (MQ=255)
                              aaCGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAATCAACTGCACCAGTTGATCGATACGATCCTGCTc                                                                                             >  1:949206/1‑134 (MQ=255)
                               aCGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAATCAACTGCACCAGTTGATCGATACGATCCTGCTCCAGCAgg                                                                                      >  1:1016218/1‑140 (MQ=255)
                                cGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAATCAACTGCACCAGTTGATCGATACGATCCTGCTCCAGCAGGt                                                                                     <  2:95110/140‑1 (MQ=255)
                                            gagCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAATCAACTGCACCAGTTGATCGATACGATCCTGCTCCAGCAGGTCTTccgcc                                                                             <  2:523210/136‑1 (MQ=255)
                                            gagCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAATCAACTGCACCAGTTGATCGATACGATCCTGCTCCAGCAGGTCTTccgcc                                                                             >  1:523210/1‑136 (MQ=255)
                                               cACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAATCAACTGCACCAGTTGATCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGtt                                                                      >  2:368100/1‑140 (MQ=255)
                                               cACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAATCAACTGCACCAGTTGATCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGtt                                                                      >  1:280483/1‑140 (MQ=255)
                                                 cgcgCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTGGCCAACCAACTGCACCAGTTGATCGATACGGTCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGc                                                                    >  2:914939/1‑140 (MQ=255)
                                                 cgcgCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAATCAACTGCACCAGTTGATCGATACGATCCTGCTCCAGCAGGTCTTccgc                                                                              >  1:1023259/1‑130 (MQ=255)
                                                 cgcgCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAATCAACTGCACCAGTTGATCGATACGATCCTGCTCCAGCAGGTCTTccgc                                                                              <  2:1023259/130‑1 (MQ=255)
                                                 cgcgCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAATCAACTGCACCAGTTGATCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGc                                                                    >  1:230850/1‑140 (MQ=255)
                                                 cgcgCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAATCAACTGCACCAGTTGATCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGc                                                                    >  1:781768/1‑140 (MQ=255)
                                                 cgcgCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAATCAACTGCACCAGTTGATCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGc                                                                    >  1:417498/1‑140 (MQ=255)
                                                 cgcgCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAATCAACTGCACCAGTTGATCGATACGATCCTGCT‑CAGCAGGTCTTCCGCCCGCTGTTGCt                                                                   >  1:887331/1‑140 (MQ=255)
                                                   cgcAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAATCAACTGCACCAGTTGATCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTg                                                                  >  1:523384/1‑140 (MQ=255)
                                                   cgcAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAATCAACTGCACCAGTTGATCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTg                                                                  >  2:190001/1‑140 (MQ=255)
                                                             gccgccCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAATCAACTGCACCAGTTGATCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGc                                                        >  2:785694/1‑140 (MQ=255)
                                                             gccgccCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAATCAACTGCACCAGTTGATCGATACGATCCTGCT‑CAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGcc                                                       <  2:887331/140‑1 (MQ=255)
                                                              cggccCCTGGCGGAAGCTGCGGTCCTGATGAGAACCCAGCATTTGCCGGACAGTCGTTTTGTCGCCAAGCAACTGCAGCAGTTGATCGAGACGGTCCTGCTCCAGCAGGTCTTCCGCCCGGTGTTGCTGAAGCGCCAGcc                                                       <  2:523384/138‑1 (MQ=255)
                                                              ccgccCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAATCAACTGCACCAGTTGATCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGcc                                                       <  1:190001/140‑1 (MQ=255)
                                                              ccgccCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAATCAACTGCACCAGTTGATCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGcc                                                       >  1:1073878/1‑140 (MQ=255)
                                                              ccgccCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAATCAACTGCACCAGTTGATCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGcc                                                       <  2:703995/140‑1 (MQ=255)
                                                              ccgccCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAATCAACTGCACCAGTTGATCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGcc                                                       <  1:772454/140‑1 (MQ=255)
                                                              ccgccCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAATCAACTGCACCAGTTGATCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGcc                                                       <  2:1073878/140‑1 (MQ=255)
                                                              ccgccCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAATCAACTGCACCAGTTGATCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGcc                                                       <  1:368100/140‑1 (MQ=255)
                                                                gccCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAATCAACTGCACCAGTTGATCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCg                                                     <  1:625223/140‑1 (MQ=255)
                                                                       gCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAATCAACTGCACCAGTTGATCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATaga                                              <  1:751927/140‑1 (MQ=255)
                                                                         ggAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAATCAACTGCACCAGTTGATCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAgg                                            <  2:1009249/140‑1 (MQ=255)
                                                                           aaGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAATCAACTGCACCAGTTGATCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGaa                                          <  2:365478/140‑1 (MQ=255)
                                                                                 cGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAATCAACTGCACCAGTTGATCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAg                                    >  1:664393/1‑140 (MQ=255)
                                                                                 cGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAATCAACTGCACCAGTTGATCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAg                                    >  1:1135582/1‑140 (MQ=255)
                                                                                 cGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAATCAACTGCACCAGTTGATCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAg                                    >  2:655989/1‑140 (MQ=255)
                                                                                  ggCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAATCAACTGCACCAGTTGATCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGa                                   <  2:230850/140‑1 (MQ=255)
                                                                                    cccAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAATCAACTGCACCAGTTGATCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGAcg                                 <  1:747234/140‑1 (MQ=255)
                                                                                      cAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAATCAACTGCACCAGTTGATCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGAcgcg                               <  2:781768/140‑1 (MQ=255)
                                                                                                   aGCATTTGCCGCACAGTCGCTTTGTCGCCAATCAACTGCACCAGTTGATCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACcgcg                  >  1:87949/1‑140 (MQ=255)
                                                                                                   aGCATTTGCCGCACAGTCGCTTTGTCGCCAATCAACTGCACCAGTTGATCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACcgcg                  >  2:1077685/1‑140 (MQ=255)
                                                                                                         tGCCGCACAGTCGCTTTGTCGCCAATCAACTGCACCAGTTGATCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCGCGCCCGAc            <  1:785694/140‑1 (MQ=255)
                                                                                                           ccGCACAGTCGCTTTGTCGCCAATCAACTGCACCAGTTGATCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCGCGCCCGACtt          >  2:1078204/1‑140 (MQ=255)
                                                                                                                   tCGCTTTGTCGCCAATCAACTGCACCAGTTGATCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCGCGCCCGACTTCCGCTTcc  <  2:87949/140‑1 (MQ=255)
                                                                                                                   tCGCTTTGTCGCCAATCAACTGCACCAGTTGATCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCGCGCCCGACTTCCGCTTcc  >  1:431767/1‑140 (MQ=255)
                                                                                                                   tCGCTTTGTCGCCAATCAACTGCACCAGTTGATCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCGCGCCCGACTTCCGCTTc   <  1:1125876/139‑1 (MQ=255)
                                                                                                                   tCGCTTTGTCGCCAATCAACTGCACCAGTTGATCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCGCGCCCGACTTCCGCTTc   >  2:1125876/1‑139 (MQ=255)
                                                                                                                                  |                                                                                                                            
CGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACCAGTTGATCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCGCGCCCGACTTCCGCTTCC  >  NC_000913/76881‑77135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑