Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,224,024	A→G	intergenic (+117/+255)	ycgI → / ← minE	protein YcgI/Z‑ring positioning protein MinE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,224,024	0	A	G	100.0%	80.7 / NA	24	intergenic (+117/+255)	ycgI/minE	protein YcgI/Z‑ring positioning protein MinE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (3/21);  total (3/21)

TGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGA‑ACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAG  >  NC_000913/1223891‑1224152                                                                                                                                       |                                                                                                                                 tgAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGA‑GCAATGaa                                                                                                                           <  2:1046727/140‑1 (MQ=255)        gTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAAACCCATCCTATTGGGGA‑GGAAAGAATAAAGaa                                                                                                                    >  2:356272/1‑140 (MQ=255)                gACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGA‑GCAATGAATAATGAAATTGCggg                                                                                                            <  2:675158/140‑1 (MQ=255)                     tCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGA‑GCAATGAATAATGAAATTGCGGGGTTat                                                                                                       <  1:59341/140‑1 (MQ=255)                     tCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAAACTCATCCTATTGGGGA‑GCAAAGAAAAAAGAAAATTCGGGGGTat                                                                                                       >  2:771749/1‑140 (MQ=255)                       aaCGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAAACCCATCCTATGGGAGA‑GCAAAGAATAATGAAAATGCGGGGGTatca                                                                                                     >  2:450148/1‑140 (MQ=255)                         cGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGA‑GCAATGAATAATGAAATTGCGGGGTTatcatc                                                                                                   <  1:722172/140‑1 (MQ=255)                          gATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGA‑GCAATGAATAATGAAATTGCGGGGTTATCAtct                                                                                                  <  2:322716/140‑1 (MQ=255)                           aTTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGA‑GCAATGAATAATGAAATTGCGGGGTTATCAtctc                                                                                                 <  1:274004/140‑1 (MQ=255)                           aTTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGA‑GCAATGAATAATGAAATTGCGGGGTTATCAtctc                                                                                                 <  2:177543/140‑1 (MQ=255)                               acaggcgcTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGA‑GCAATGAATAATGAAATTGCGGGGTTATCATCTCCCAg                                                                                             <  1:267800/136‑1 (MQ=255)                               aaCTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGA‑GCAATGAATAATGAAATTGCGGGGTTATCATCTCCCAg                                                                                             <  2:654639/140‑1 (MQ=255)                               aaCTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGA‑GCAATGAATAATGAAATTGCGGGGTTATCATCTCCCAg                                                                                             <  1:1095941/140‑1 (MQ=255)                                aCTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGA‑GCAATGAATAATGAAATTGCGGGGTTATCACCTCCCAGt                                                                                            <  1:1114010/140‑1 (MQ=255)                                   gcgcTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGA‑GCAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGtata                                                                                         <  1:751325/140‑1 (MQ=255)                                              cAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAAACTCCTCCTATTGGGGA‑GGAAAGAAAAATGAAAATGCGGGGGTATTATCTCCCAGTATATTCATAcccgt                                                                              >  2:267800/1‑136 (MQ=255)                                                                  ttGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGGAAACTCCTCCTATGGGAGG‑GGAATGAATAATGAAATTGCGGGGGTATTATCTCCCCGTATATCCATACTAAAAAAAAAGGTATTTTTTCAAc                                                          >  2:788599/1‑140 (MQ=255)                                                                  ttGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAACCCCCTCCCATGGGGGA‑GCAAAGAAAAAAGAAAATGCGGGGGTTTCATCTCCCCGCATATCCCTACTAACAATAAAGTTATTTTATCAAc                                                          >  2:716755/1‑140 (MQ=255)                                                                          gAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAAACTCATCCTTTTGGGGA‑GGAAAGAAAAATAAAAATTCGGGGGTATCATCTCCCCCTATATTCATACTAAAAAAAAAGATATTTATTTAAACAAGCATa                                                  >  2:977967/1‑140 (MQ=255)                                                                          gAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAAAAACCTTCTATTGGGGAGGAAAGGAAAAAAAAAATTG‑GGGGGTATGATCTCCCCATATATTCCAACTTAAAAAAAAATTTTTTTTTCAACCAGGCCTa                                                  >  2:96163/1‑140 (MQ=255)                                                                               ttCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGA‑GCAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACAt                                             <  1:931023/140‑1 (MQ=255)                                                                                                   gCCAGTTACGGCGGGAAACCTCATCCTATGGGAGA‑GCAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGaa                          <  1:977967/140‑1 (MQ=255)                                                                                                   gCCAGTTACGGCGGGAAACCTCATCCTATGGGAGA‑GCAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGaa                          <  1:576416/140‑1 (MQ=255)                                                                                                        ttACGGCGGGAAACCTCATCCTATGGGAGA‑GCAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGcc                     <  2:817571/140‑1 (MQ=255)                                                                                                             gcggGAAACCTCATCCTATGGGAGA‑GCAATGAATAATGAAATTGCGGGTTTATAATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAg                <  1:1050259/140‑1 (MQ=255)                                                                                                                 gAAACCTCATACTATGGGAGA‑GCAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGgtgt            <  1:788599/140‑1 (MQ=255)                                                                                                                        cATCCTATGGGAGA‑GCAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAgggg     <  1:771749/140‑1 (MQ=255)                                                                                                                        cATCCTATGGGAGA‑GCAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAgggg     >  1:1051631/1‑140 (MQ=255)                                                                                                                          tCCTATGGGAGA‑GCAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGa   >  1:198776/1‑140 (MQ=255)                                                                                                                           ccTATGGGAGA‑GCAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAg  <  2:198776/140‑1 (MQ=255)                                                                                                                           ccTATGGGAGA‑GCAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAg  <  1:716755/140‑1 (MQ=255)                                                                                                                           ccTATGGGAGA‑GCAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAg  >  1:349914/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                 TGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGA‑ACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAG  >  NC_000913/1223891‑1224152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑