Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,249,084	T→C	S11G (AGC→GGC)	dhaM ←	dihydroxyacetone kinase subunit M

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,249,084	0	T	C	100.0%	208.7 / NA	60	S11G (AGC→GGC)	dhaM	dihydroxyacetone kinase subunit M
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/29);  total (31/29)

ATGGCCTCCATCACTTTGACGGCATCGGTACCAATGGGATTTTGTGGATCGTCAATTCCCGCGGCAATGGCGATTTTACAACTATCACTCATTAACATCTGACGGGCTAATTCACCGACACCTTCTCCCAGTCGGCTGCTATGTGAAACTATGACCAGGTTTACCATCACCAATTCCTTACTCTTTTGCGGCTAACGCCAACATTTGCATCATAAACATCACCGAGGTCGCGCCGGGATCCTGGTGACCAATACTGCGTTCACCGAGATAA  >  NC_000913/1248948‑1249218                                                                                                                                         |                                                                                                                                       aTGGCCTCCATCACTTTGACGGCATCGGTACCAATGGGATTTTGTGGATCGTCAATTCCCGCGGCAATGGCGATTTTACAACTATCACTCATTAACATCTGACGGGCTAATTCACCGACACCTTCTCCCAGTCGgccgct                                                                                                                                     <  1:168448/140‑1 (MQ=255) aTGGCCTCCATCACTTTGACGGCATCGGTACCAATGGGATTTTGTGGATCGTCAATTCCCGCGGCAATGGCGATTTTACAACTATCACTCATTAACATCTGACGGGCTAATTCACCGACACCTTCTCCCAGTCGgccgct                                                                                                                                     >  1:834921/1‑140 (MQ=255)      cTCCATCACTTTGACGGCATCGGTACCAATGGGATTTTGTGGATCGTCAATTCCCGCGGCAATGGCGATTTTACAACTATCACTCATTAACATCTGACGGGCTAATTCACCGACACCTTCTCCCAGTCGGCCGCTAtgtg                                                                                                                                <  2:391574/140‑1 (MQ=255)         cATCACTTTGACGGCATCGGTACCAATGGGATTTTGTGGATCGTCAATTCCCGCGGCAATGGCGATTTTACAACTATCACTCATTAACATCTGACGGGCTAATTCACCGACACCTTCTCCCAGTCGGCCGCTATGTGaaa                                                                                                                             <  1:1038781/140‑1 (MQ=255)                   aCGGCATCGGTACCAATGGGATTTTGTGGATCGTCAATTCCCGCGGCAATGGCGATTTTACAACTATCACTCATTAACATCTGACGGGCTAATTCACCGACACCTTCTCCCAGTCGGCCGCTATGTGAAACTATGACCAg                                                                                                                   >  2:55074/1‑140 (MQ=255)                     ggCATCGGTACCAATGGGATTTTGTGGATCGTCAATTCCCGCGGCAATGGCGATTTTACAACTATCACTCATTAACATCTGACGGGCTAATTCACCGACACCTTCTCCCAGTCGGCCGCTATGTGAAACTATGACCAGGt                                                                                                                 >  1:785912/1‑140 (MQ=255)                     ggCATCGGTACCAATGGGATTTTGTGGATCGTCAATTCCCGCGGCAATGGCGATTTTACAACTATCACTCATTAACATCTGACGGGCTAATTCACCGACACCTTCTCCCAGTCGGCCGCTATGTGAAACTATGACCAGGt                                                                                                                 <  1:5635/140‑1 (MQ=255)                         tCGGTACCAATGGGATTTTGTGGATCGTCAATTCCCGCGGCAATGGCGATTTTACAACTATCACTCATTAACATCTGACGGGCTAATTCACCGACACCTTCTCCCAGTCGGCCGCTATGTGAAACTATGACCAGGTTTAc                                                                                                             <  2:232945/140‑1 (MQ=255)                          cGGTACCAATGGGATTTTGTGGATCGTCAATTCCCGCGGCAATGGCGATTTTACAACTATCACTCATTAACATCTGACGGGCTAATTCACCGACACCTTCTCCCAGTCGGCCGCTATGTGAAACTATGACCAGGTTTAcc                                                                                                            <  2:591743/140‑1 (MQ=255)                             tACCAATGGGATTTTGTGGATCGTCAATTCCCGCGGCAATGGCGATTTTACAACTATCACTCATTAACATCTGACGGGCTAATTCACCGACACCTTCTCCCAGTCGGCCGCTATGTGAAACTATGACCAGGTTTACCATc                                                                                                         <  1:55074/140‑1 (MQ=255)                                  aTGGGATTTTGTGGATCGTCAATTCCCGCGGCAATGGCGATTTTACAACTATCACTCATTAACATCTGACGGGCTAATTCACCGACACCTTCTCCCAGTCGGCCGCTATGTGAAACTATGACCAGGTTTACCATCACCaa                                                                                                    >  1:780109/1‑140 (MQ=255)                                    gggATTTTGTGGATCGTCAATTCCCGCGGCAATGGCGATTTTACAACTATCACTCATTAACATCTGACGGGCTAATTCACCGACACCTTCTCCCAGTCGGCCGCTATGTGAAACTATGACCAGGTTTACCATCACCAAtt                                                                                                  <  2:834921/140‑1 (MQ=255)                                      gATTTTGTGGATCGTCACTTCCCGCGGCAATGGCGATTTTACAACTATCACTCATTAACATCTGACGGGCTAATTCACCGACACCTTCTCCCAGTCTGCCGCTATGTGAAACTATGACCAGGTTTACCATCACCAATTcc                                                                                                <  2:1023597/140‑1 (MQ=255)                                           tgtgGATCGTCAATTCCCGCGGCAATGGCGATTTTACAACTATCACTCATTAACATCTGACGGGCTAATTCACCGACACCTTCTCCCAGTCGGCCGCTATGTGAAACTATGACCAGGTTTACCATCAc                                                                                                       <  2:329483/128‑1 (MQ=255)                                           tgtgGATCGTCAATTCCCGCGGCAATGGCGATTTTACAACTATCACTCATTAACATCTGACGGGCTAATTCACCGACACCTTCTCCCAGTCGGCCGCTATGTGAAACTATGACCAGGTTTACCATCAc                                                                                                       >  1:329483/1‑128 (MQ=255)                                                      aaTTCCCGCGGCAATGGCGATTTTACAACTATCACTCATTAACATCTGACGGGCTAATTCACCGACACCTTCTCCCAGTCGGCCGCTATGTGAAACTATGACCAGGTTTACCATCACCAATTCCTTACTCTTTTGCGGCt                                                                                <  2:774209/140‑1 (MQ=255)                                                        ttCCCGCGGCAATGGCGATTTTACAACTATCACTCGTTAACATCTGACGGGCTAATTCACCGACACCTTCTCCCAGTCGGCCGCTATGTGAAACTATGACCAGGTTTACCATCACCAATTCCTTACTCTTTTGCGGCTaa                                                                              <  1:36724/140‑1 (MQ=255)                                                            cgcgGCAATGGCGATTTTACAACTATCACTCATTAACATCTGACGGGCTAATTCACCGACACCTTCTCCCAGTCGCCCGCTATGTGAAACTATGACCAGGTTTACCATCACCAATTCCTTACTCTTTTGCGGCTAACGcc                                                                          <  2:785912/140‑1 (MQ=255)                                                              cgGCAATGGCGATTTTACAACTATCACTCATTAACATCTGACGGGCTAATTCACCGACACCTTCTCCCAGTCGGCCGCTATGTGAAACTATGACCAGGTTTACCATCACCAATTCCTTACTCTTTTGCGGCTAACGCCaa                                                                        >  1:532233/1‑140 (MQ=255)                                                               ggCAATGGCGATTTTACAACTATCACTCATTAACATCTGACGGGCTAATTCACCGACACCTTCTCCCAGTCGGCCGCTATGTGAAACTATGACCAGGTTTACCATCACCAATTCCTTACTCTTTTGCGGCTAACGCCAAc                                                                       >  1:702944/1‑140 (MQ=255)                                                                    tGGCGATTTTACAACTATCACTCATTAACATCTGACGGGCTAATTCACCGACACCTTCTCCCAGTCGGCCGCTATGTGAAACTATGACCAGGTTTACCATCACCAATTCCTTACTCTTTTGCGGCTAACGCCAACATTTg                                                                  <  2:780109/140‑1 (MQ=255)                                                                        gATTTTACAACTATCACTCATTAACATCTGACGGGCTAATTCACCGACACCTTCTCCCAGTCGGCCGCTATGTGAAACTATGACCAGGTTTACCATCACCAATTCCTTGCTCTTTTGCGGCTAACGCCAACATTTGcatc                                                              <  2:518197/140‑1 (MQ=255)                                                                              aCAACTATCACTCATTAACATCTGACGGGCTAATTCACCGACACCTTCTCCCAGTCGGCCGCTATGTGAAACTATGACCAGGTTTACCATCACCAATTCCTTACTCTTTTGCGGCTAACGCCAACATTTGCATCATAAAc                                                        <  2:702944/140‑1 (MQ=255)                                                                                     tCACTCATTAACATCTGACGGGCTAATTCACCGACACCTTCTCCCAGTCGGCCGCTATGTGAAACTATGACCAGGTTTACCATCACCAATTCCTTACTCTTTTGCGGCTAACGCCAACATTTGCATCATAAACATCACCg                                                 >  1:530151/1‑140 (MQ=255)                                                                                       aCTCATTAACATCTGACGGGCTAATTCACCGACACCTTCTCCCAGTCGGCCGCTATGTGAAACTATGACCAGGTTTACCATCACCAATTCCTTACTCTTTTGCGGCTAACGCCAACATTTGCATCATAAACATCACCGAg                                               >  1:338574/1‑140 (MQ=255)                                                                                       aCTCATTAACATCTGACGGGCTAATTCACCGACACCTTCTCCCAGTCGGCCGCTATGTGAAACTATGACCAGGTTTACCATCACCAATTCCTTACTCTTTTGCGGCTAACGCCAACATTTGCATCATAAACATCACCGAg                                               >  2:208758/1‑140 (MQ=255)                                                                                         tCATTAACATCTGACGGGCTAATTCACCGACACCTTCTCCCAGTCGGCCGCTATGTGAAACTATGACCAGGTTTACCATCACCAATTCCTTACTCTTTTGCGGCTAACGCCAACATTTGCATCATAAACATCACCGAGGt                                             <  1:901629/140‑1 (MQ=255)                                                                                         tCATTAACATCTGACGGGCTAATTCACCGACACCTTCTCCCAGTCGGCCGCTATGTGAAACTATGACCAGGTTTACCATCACCAATTCCTTACTCTTTTGCGGCTAACGCCAACATTTGCATCATAAACATCACCGAGGt                                             >  2:872667/1‑140 (MQ=255)                                                                                           aTTAACATCTGACGGGCTAATTCACCGACACCTTCTCCCAGTCGGCCGCTATGTGAAACTATGACCAGGTTTACCATCACCAATTCCTTACTCTTTTGCGGCTAACGCCAACATTTGCATCATAAACATCACCGAGGTcg                                           <  2:456521/140‑1 (MQ=255)                                                                                            ttAACATCTGACGGGCTAATTCACCGACACCTTCTCCCAGTCGGCCGCTATGTGAAACTATGACCAGGTTTACCATCACCAATTCCTTACTCTTTTGCGGCTAACGCCAACATTTGCATCATAAACATCACCGAGGTcgc                                          >  2:559614/1‑140 (MQ=255)                                                                                             tAACATCTGACGGGCTAATTCACCGACACCTTCTCCCAGTCGGCCGCTATGTGAAACTATGACCAGGTTTACCATCACCAATTCCTTACTCTTTTGCGGCTAACGCCAACATTTGCATCATAAACATCACCGAGGTcgcg                                         >  1:656025/1‑140 (MQ=255)                                                                                             tAACATCTGACGGGCTAATTCACCGACACCTTCTCCCAGTCGGCCGCTATGTGAAACTATGACCAGGTTTACCATCACCAATTCCTTACTCTTTTGCGGCTAACGCCAACATTTGCATCATAAACATCACCGAGGTcgcg                                         >  2:638971/1‑140 (MQ=255)                                                                                             tAACATCTGACGGGCTAATTCACCGACACCTTCTCCCAGTCGGCCGCTATGTGAAACTATGACCAGGTTTACCATCACCAATTCCTTACTCTTTTGCGGCTAACGCCAACATTTGCATCATAAACATCACCGAGGTcgcg                                         >  2:164559/1‑140 (MQ=255)                                                                                               aCATCTGACGGGCTAATTCACCGACACCTTCTCCCAGTCGGCCGCTATGTGAAACTATGACCAGGTTTACCATCACCAATTCCTTACTCTTTTGCGGCTAACGCCAACATTTGCATCATAAACATCACCGAGGTCGCGcc                                       >  1:616356/1‑140 (MQ=255)                                                                                                cATCTGACGGGCTAATTCACCGACACCTTCTCCCAGTCGGCCGCTATGTGAAACTATGACCAGGTTTACCATCACCAATTCCTTACTCTTTTGCGGCTAACGCCAACATTTGCATCATAAACATCACCGAGGTCGCGCCg                                      >  2:875895/1‑140 (MQ=255)                                                                                                   cTGACGGGCTAATTCACCGACACCTTCTCCCAGTCGGCCGCTATGTGAAACTATGACCAGGTTTACCATCACCAATTCCTTACTCTTTTGCGGCTAACGCCAACATTTGCATCATAAACATCACCGAGGTCGCGCCGGGa                                   >  2:490872/1‑140 (MQ=255)                                                                                                   cTGACGGGCTAATTCACCGACACCTTCTCCCAGTCGGCCGCTATGTGAAACTATGACCAGGTTTACCATCACCAATTCCTTACTCTTTTGCGGCTAACGCCAACATTTGCATCATAAACATCACCGAGGTCGCGCCGGGa                                   <  1:105146/140‑1 (MQ=255)                                                                                                   cTGACGGGCTAATTCACCGACACCTTCTCCCAGTCGGCCGCTATGTGAAACTATGACCAGGTTTACCATCACCAATTCCTTACTCTTTTGCGGCTAACGCCAACATTTGCATCATAAACATCACCGAGGTCGCGCCGGGa                                   <  1:164559/140‑1 (MQ=255)                                                                                                     gACGGGCTAATTCACCGACACCTTCTCCCAGTCGGCCGCTATGTGAAACTATGACCAGGTTTACCATCACCAATTCCTTACTCTTTTGCGGCTAACGCCAACATTTGCATCATAAACATCACCGAGGTCGCGCCGGGATc                                 >  1:35331/1‑140 (MQ=255)                                                                                                     gACGGGCTAATTCACCGACACCTTCTCCCAGTCGGCCGCTATGTGAAACTATGACCAGGTTTACCATCACCAATTCCTTACTCTTTTGCGGCTAACGCCAACATTTGCATCATAAACATCACCGAGGTCGCGCCGGGATc                                 <  1:638971/140‑1 (MQ=255)                                                                                                      aCGGGCTAATTCACCGACACCTTCTCCCAGTCGGCCGCTATGTGAAACTATGACCAGGTTTACCATCACCAATTCCTTACTCTTTTGCGGCTAACGCCAACATTTGCATCATAAACATCACCGAGGTCGCGCCGGGATcc                                >  1:410044/1‑140 (MQ=255)                                                                                                      aCGGGCTAATTCACCGACACCTTCTCCCAGTCGGCCGCTATGTGAAACTATGACCAGGTTTACCATCACCAATTCCTTACTCTTTTGCGGCTAACGCCAACATTTGCATCATAAACATCACCGAGGTCGCGCCGGGATcc                                >  2:493004/1‑140 (MQ=255)                                                                                                       cGGGCTAATTCACCGACACCTTCTCCCAGTCGGCCGCTATGTGAAACTATGACCAGGTTTACCATCACCAATTCCTTACTCTTTTGCGGCTAACGCCAACATTTGCATCATAAACATCACCGAGGTCGCGCCGGGATCCt                               >  2:1058584/1‑140 (MQ=255)                                                                                                          gCTAATTCACCGACACCTTCTCCCAGTCGGCCGCTATGTGAAACTATGACCAGGTTTACCATCACCAATTCCTTACTCTTTTGCGGCTAACGCCAACATTTGCATCATAAACATCACCGAGGTCGCGCCGGGATCCTGGt                            <  2:338574/140‑1 (MQ=255)                                                                                                           cTAATTCACCGACACCTTCTCCCAGTCGGCCGCTATGTGAAACTATGACCAGGTTTACCATCACCAATTCCTTACTCTTTTGCGGCTAACGCCAACATTTGCATCATAAACATCACCGAGGTCGCGCCGGGATCCTGGTg                           >  2:62608/1‑140 (MQ=255)                                                                                                             aaTTCACCGACACCTTCTCCCAGTCGGCCGCTATGTGAAACTATGACCAGGTTTACCATCACCAATTCCTTACTCTTTTGCGGCTAACGCCAACATTTGCATCATAAACATCACCGAGGTCGCGCCGGGATCCTGGTGAc                         >  1:1070225/1‑140 (MQ=255)                                                                                                             aaTTCACCGACACCTTCTCCCAGTCGGCCGCTATGTGAAACTATGACCAGGTTTACCATCACCAATTCCTTACTCTTTTGCGGCTAACGCCAACATTTGCATCATAAACATCACCGAGGTCGCGCCGGGATCCTGGTGAc                         <  1:875895/140‑1 (MQ=255)                                                                                                             aaTTCACCGACACCTTCTCCCAGTCGGCCGCTATGTGAAACTATGACCAGGTTTACCATCACCAATTCCTTACTCTTTTGCGGCTAACGCCAACATTTGCATCATAAACATCACCGAGGTCGCGCCGGGATCCTGGTGAc                         <  2:35331/140‑1 (MQ=255)                                                                                                                     gACACCTTCTCCCAGTCGGCCGCTATGTGAAACTATGACCAGGTTTACCGTCACCAATTCCTTACTCTTTTGCGGCTAACGCCAACATTTGCATCATAAACATCACCGAGGTCGCGCCGGGATCCTGGTGACCAATACTg                 >  1:470421/1‑140 (MQ=255)                                                                                                                       cacCTTCTCCCAGTCGGCCGCTATGTGAAACTATGACCAGGTTTACCGTCACCAATTCCTTACTCTTTTGCGGCTAACGCCAACATTTGCATCATAAACATCACCGAGGTCGCGCCGGGATCCTGGTGACCAATACTGCg               <  2:470421/140‑1 (MQ=255)                                                                                                                        acCTTCTCCCAGTTGGCCGCTATGTGAAACTATGACCAGGTTTACCATCACCAATTCCTTACTCTTTTGCGGCTAACGCCAACATTTGCATCATAAACATCACCGAGGTCGCGCCGGGATCCTGGTGACCAATACTGCGt              >  2:1070408/1‑140 (MQ=255)                                                                                                                        acCTTCTCCCAGTCGGCCGCTATGTGAAACTATGACCAGGTTTACCATCACCAATTCCTTACTCTTTTGCGGCTAACGCCAACATTTGCATCATAAACATCACCGAGGTCGCGCCGGGATCCTGGt                            <  1:745403/126‑1 (MQ=255)                                                                                                                        acCTTCTCCCAGTCGGCCGCTATGTGAAACTATGACCAGGTTTACCATCACCAATTCCTTACTCTTTTGCGGCTAACGCCAACATTTGCATCATAAACATCACCGAGGTCGCGCCGGGATCCTGGt                            >  2:745403/1‑126 (MQ=255)                                                                                                                            tctcCCAGTCGGCCGCTATGTGAAACTATGACCAGGTTTACCATCACCAATTCCTTACTCTTTTGCGGCTAACGCCAACATTTGCATCATAAACATCACCGAGGTCGCGCCGGGATCCTGGTGACCAATACTGCGTTCa           <  1:798127/139‑1 (MQ=255)                                                                                                                            tctcCCAGTCGGCCGCTATGTGAAACTATGACCAGGTTTACCATCACCAATTCCTTACTCTTTTGCGGCTAACGCCAACATTTGCATCATAAACATCACCGAGGTCGCGCCGGGATCCTGGTGACCAATACTGCGTTCa           >  2:798127/1‑139 (MQ=255)                                                                                                                            tctcCCAGTCGGCCGCTATGTGAAACTATGACCAGGTTTACCATCACCAATTCCTTACTCTTTTGCGGCTAACGCCAACATTTGCATCATAAACATCACCGAGGTCGCGCCGGGATCCTGGTGACCAATACTGCGTTCAc          >  1:453230/1‑140 (MQ=255)                                                                                                                             ctcCCAGTCGGCCGCTATGTGAAACTATGACCAGGTTTACCATCACCAATTCCTTACTCTTTTGCGGCTAACGCCAACATTTGCATCATAAACATCACCGAGGTCGCGCCGGGATCCTGGTGACCAATACTGCGTTCAcc         <  2:861138/140‑1 (MQ=255)                                                                                                                                    tCGGCCGCTATGTGAAACTATGACCAGGTTTACCATCGCCAATTCCTTACTCTTTTGCGGCTAACGCCAACATTTGCATCATAAACATCACCGAGGTCGCGCCGGGACCCTGGTGACCAATACTGCGTTCACCGAGATaa  >  2:1016188/1‑140 (MQ=255)                                                                                                                                    tCGGCCGCTATGTGAAACTATGACCAGGTTTACCATCACCAATTCCTTACTCTTTTGCGGCTAACGCGAACATTTGCATCATAAACATCACCGAGGTCGCGCCGGGATCCTGGTGACCAATACTGCGTTCACCGAGATaa  >  2:1045254/1‑140 (MQ=255)                                                                                                                                    tCGGCCGCTATGTGAAACTATGACCAGGTTTACCATCACCAATTCCTTACTCTTTTGCGGCTAACGCCAACATTTGCATCATAAACATCACCGAGGTCGCGCCGGGATCCTGGTGACCAATACTGCGTTCACCGAGATaa  <  2:530151/140‑1 (MQ=255)                                                                                                                                    tCGGCCGCTATGTGAAACTATGACCAGGTTTACCATCACCAATTCCTTACTCTTTTGCGGCTAACGCCAACATTTGCATCATAAACATCACCGAGGTCGCGCCGGGATCCTGGTGACCAATACTGCGTTCACCGAGATaa  <  1:323579/140‑1 (MQ=255)                                                                                                                                    tCGGCCGCTATGTGAAACTATGACCAGGTTTACCATCACCAATTCCTTACTCTTTTGCGGCTAACGCCAACATTTGCATCATAAACATCACCGAGGTCGCGCCGGGATCCTGGTGACCAATACTGCGTTCACCGAGATaa  >  2:323579/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       ATGGCCTCCATCACTTTGACGGCATCGGTACCAATGGGATTTTGTGGATCGTCAATTCCCGCGGCAATGGCGATTTTACAACTATCACTCATTAACATCTGACGGGCTAATTCACCGACACCTTCTCCCAGTCGGCTGCTATGTGAAACTATGACCAGGTTTACCATCACCAATTCCTTACTCTTTTGCGGCTAACGCCAACATTTGCATCATAAACATCACCGAGGTCGCGCCGGGATCCTGGTGACCAATACTGCGTTCACCGAGATAA  >  NC_000913/1248948‑1249218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑