Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,646,951	A→G	intergenic (+214/+223)	flxA → / ← ydfW	Qin prophage; protein FlxA/Qin prophage; uncharacterized protein YdfW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,646,951	0	A	G	100.0%	277.3 / NA	79	intergenic (+214/+223)	flxA/ydfW	Qin prophage; protein FlxA/Qin prophage; uncharacterized protein YdfW
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (44/35);  total (44/35)

TTAACATTACAAGATATAAGTAATGGACGCACTCCCAATTAGTCTATTTAAATCGCCACGAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGACAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCTATTTACCATAACGCCTCATTACGCGCACCGCCCAGACTGACTCAGCGCGTTTCTGGCATATCCCCGGTAAAAC  >  NC_000913/1646827‑1647081                                                                                                                             |                                                                                                                                   ttAACATTACAAGATATAAGTAATGGACGCACTCCCAATTAGTCTATTTAAATCGCCACGAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTg                                                                                                                     <  2:861568/140‑1 (MQ=255)  tAACATTACAAGATATAAGTAATGGACGCACTCCCAATTAGTCTATTTAAATCGCCACGAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGc                                                                                                                    >  2:667087/1‑140 (MQ=255)  tAACATTACAAGATATAAGTAATGGACGCACTCCCAATTAGTCTATTTAAATCGCCACGAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGc                                                                                                                    >  2:84512/1‑140 (MQ=255)   aaCATTACAAGATATAAGTAATGGACGCACTCCCAATTAGTCTATTTAAATCGCCACGAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGcc                                                                                                                   <  1:767192/140‑1 (MQ=255)   aaCATTACAAGATATAAGTAATGGACGCACTCCCAATTAGTCTATTTAAATCGCCACGAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGcc                                                                                                                   <  1:667087/140‑1 (MQ=255)       ttACAAGATATAAGTAATGGACGCACTCCCAATTAGTCTATTTAAATCGCCACGAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACt                                                                                                               >  1:658037/1‑140 (MQ=255)         aCAAGATATAAGTAATGGACGCACTCCCAATTAGTCTATTTAAATCGCCACGAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCTACAAAACTGCCAACTTc                                                                                                             >  2:721111/1‑140 (MQ=255)         aCAAGATATAAGTAATGGACGCACTCCCAATTAGTCTATTTAAATCGCCACGAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTc                                                                                                             <  2:336709/140‑1 (MQ=255)          cAAGATATAAGTAATGGACGCACTCCCAATTAGTCTATTTAAATCGCCACGAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTcc                                                                                                            >  1:952308/1‑140 (MQ=255)                       aTGGACGCACTCCCAATTAGTCTATTTAAATCGCCACGAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCtt                                                                                               >  2:546044/1‑140 (MQ=255)                       aTGGACGCACTCCCAATTAGTCTATTTAAATCGCCACGAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCtt                                                                                               >  1:216083/1‑140 (MQ=255)                        tGGACGCACTCCCAATTAGTCTATTTAAATCGCCACGAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTa                                                                                              >  2:158037/1‑140 (MQ=255)                        tGGACGCACTCCCAATTAGTCTATTTAAATCGCCACGAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTa                                                                                              >  1:919605/1‑140 (MQ=255)                        tGGACGCACTCCCAATTAGTCTATTTAAATCGCCACGAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTa                                                                                              >  1:966432/1‑140 (MQ=255)                        tGGACGCACTCCCAATTAGTCTATTTAAATCGCCACGAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTa                                                                                              >  2:470718/1‑140 (MQ=255)                        tGGACGCACTCCCAATTAGTCTATTTAAATCGCCACGAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTa                                                                                              >  1:252130/1‑140 (MQ=255)                         ggACGCACTCCCAATTAGTCTATTTAAATCGCCACGAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAg                                                                                             <  2:252130/140‑1 (MQ=255)                         ggACGCACTCCCAATTAGTCTATTTAAATCGCCACGAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAg                                                                                             >  2:473921/1‑140 (MQ=255)                         ggACGCACTCCCAATTAGTCTATTTAAATCGCCACGAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAg                                                                                             >  1:246016/1‑140 (MQ=255)                            cGCACTCCCAATTAGTCTATTTAAATCGCCACGAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCgg                                                                                          >  1:519834/1‑140 (MQ=255)                              cACTCCCAATTAGTCTATTTAAATCGCCACGAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCg                                                                                           >  2:167316/1‑137 (MQ=255)                              cACTCCCAATTAGTCTATTTAAATCGCCACGAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCg                                                                                           <  1:167316/137‑1 (MQ=255)                               aCTCCCAATTAGTCTATTTAAATCGCCACGAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACa                                                                                       >  2:951690/1‑140 (MQ=255)                               aCTCCCAATTAGTCTATTTAAATCGCCACGAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACa                                                                                       <  1:473921/140‑1 (MQ=255)                                 tCCCAATTAGTCTATTTAAATCGCCACGAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACAtt                                                                                     >  2:856587/1‑140 (MQ=255)                                  cccAATTAGTCTATTTAAATCGCCACGAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGctct                                                                                                >  1:424795/1‑128 (MQ=255)                                  cccAATTAGTCTATTTAAATCGCCACGAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGctct                                                                                                <  2:424795/128‑1 (MQ=255)                                     aaTTAGTCTATTTAAATCGCCACGAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCa                                                                                 <  1:694291/140‑1 (MQ=255)                                         aGTCTATTTAAATCGCCACGAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAgg                                                                             >  2:171805/1‑140 (MQ=255)                                         aGTCTATTTAAATCGCCACGAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAgg                                                                             <  1:158037/140‑1 (MQ=255)                                           tCTATTTAAATCGCCACGAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCg                                                                                           >  1:938955/1‑124 (MQ=255)                                           tCTATTTAAATCGCCACGAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCg                                                                                           <  2:938955/124‑1 (MQ=255)                                           tCTATTTAAATCGCCACGAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCt                                                                           >  2:143426/1‑140 (MQ=255)                                           tCTATTTAAATCGCCACGAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCt                                                                           >  2:910033/1‑140 (MQ=255)                                              aTTTAAATCGCCACGAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCTAtt                                                                        <  1:910033/140‑1 (MQ=255)                                               tttAAATCGCCACGAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCTAttt                                                                       <  1:951690/140‑1 (MQ=255)                                                   aaTCGCCACGAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCTATTTACCa                                                                   <  2:658037/140‑1 (MQ=255)                                                   aaTCGCCACGAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCTATTTACCa                                                                   <  1:745812/140‑1 (MQ=255)                                                    aTCGCCACGAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCTATTTACCAt                                                                  >  2:691324/1‑140 (MQ=255)                                                     tCGCCACGAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCTATTTACCATa                                                                 <  2:519834/140‑1 (MQ=255)                                                     tCGCCACGAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCTATTTACCATa                                                                 <  1:171805/140‑1 (MQ=255)                                                       gCCACGAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCTATTTACCATAAc                                                               >  1:638071/1‑140 (MQ=255)                                                        ccACGAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCTATTTACCATAACg                                                              <  2:216083/140‑1 (MQ=255)                                                         cACGAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCTATTTACCATAACGc                                                             >  1:389074/1‑140 (MQ=255)                                                         cACGAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCTATTTACCATAACGc                                                             >  1:223643/1‑140 (MQ=255)                                                            gAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCTATTTACCATAACGCCTc                                                          <  2:451274/140‑1 (MQ=255)                                                                     tGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCTATTTACCATAACGCCTCATTACgcgc                                                 <  2:638071/140‑1 (MQ=255)                                                                     tGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCTATTTACCATAACGCCTCATTACgcgc                                                 <  2:952308/140‑1 (MQ=255)                                                                     tGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCTATTTACCATAACGCCTCATTACgcgc                                                 <  1:421848/140‑1 (MQ=255)                                                                     tGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCACAGGCTATTTACCATAACGCCTCATTACgcgc                                                 <  1:856587/140‑1 (MQ=255)                                                                       aCAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCTATTTACCATAACGCCTCATTACGCGCAc                                               >  1:612738/1‑140 (MQ=255)                                                                         aaCCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCTATTTACCATAACGCCTCATTACGCGCa                                                >  2:781868/1‑137 (MQ=255)                                                                         aaCCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCTATTTACCATAACGCCTCATTACGCGCa                                                <  1:781868/137‑1 (MQ=255)                                                                           cccATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCTATTTACCATAACGCCTCATTACGCGCACCGcc                                           <  1:143426/140‑1 (MQ=255)                                                                              aTGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCTATTTACCATAACGCCTCATTACGCGCACCGCCCAg                                        >  1:478982/1‑140 (MQ=255)                                                                                 aTCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCTATTTACCATAACGCCTCATTACGCGCACCGCCCAgact                                     >  1:955602/1‑140 (MQ=255)                                                                                     aTTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCTATTTACCATAACGCCTCATTACGCGCACCGCCCAgactgact                                 >  1:327357/1‑140 (MQ=255)                                                                                      ttATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCTATTTACCATAACGCCTCATTACgcgc                                                 >  1:696916/1‑123 (MQ=255)                                                                                      ttATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCTATTTACCATAACGCCTCATTACgcgc                                                 <  2:696916/123‑1 (MQ=255)                                                                                      ttATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCTATTTACCATAACGCCTCATTACGCGCACCGCCCAGACTGACTc                                >  1:628457/1‑140 (MQ=255)                                                                                      ttATGAATTGCAACTATTTCTGTAGTCACTTTTGCGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCTATTTACCATAACGCCTCATTACGCGCACCGCCCAGACTGACTc                                >  1:109853/1‑140 (MQ=255)                                                                                         tGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCTATTTACCATAACGCCTCATTACGCGCACCGCCCAGACTGACTCAgc                             <  2:223643/140‑1 (MQ=255)                                                                                          gAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAGCTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCTATTTACCATAACGCCTCATTACGCGCACCGCCCAGACTGACTCAgcg                            <  1:28052/140‑1 (MQ=255)                                                                                          gAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCTATTTACCATAACGCCTCATTACGCGCACCGCCCAGACTGACTCAgcg                            <  2:966432/140‑1 (MQ=255)                                                                                          gAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCTATTTACCATAACGCCTCATTACGCGCACCGCCCAGACTGACTCAgcg                            <  1:691324/140‑1 (MQ=255)                                                                                            aTTGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCTATTTACCATAACGCCTCATTACGCGCACCGCCCAGACTGACTCAGcgcg                          <  2:563915/140‑1 (MQ=255)                                                                                              tGCAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCTATTTACCATAACGCCTCATTACGCGCACCGCCCAGACTGACTCAGCGCGtt                        >  2:576963/1‑140 (MQ=255)                                                                                                cAACTATTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCTATTTACCATAACGCCTCATTACGCGCACCGCCCAGACTGACTCAGCGCGTTTc                      >  2:69415/1‑140 (MQ=255)                                                                                                  aCTATCTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCTATTTACCATAACGCCTCATTACGCGCACCGCCCAGACTGACTCAGCGCGTTTCTg                    >  1:641470/1‑140 (MQ=255)                                                                                                     aTTTCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCTATTTACCATAACGCCGCATTACGCGCACCGCCCAGACTGACTCAGCGCGTTTCTGGCa                 <  1:470718/140‑1 (MQ=255)                                                                                                       ttCTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCTATTTACCATAACGCCTCATTACGCGCACCGCCCAGACTGACTCAGCGCGTTTCTGGCata               >  2:357798/1‑140 (MQ=255)                                                                                                         cTGTAGTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCTATTTACCATAACGCCTCATTACGCGCACCGCCCAGACTGACTCAGCGCGTTTCTGGCATATc             <  2:109853/140‑1 (MQ=255)                                                                                                              gTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCTATTTACCATAACGCCTCATTACGCGCACCGCCCAGACTGACTCAGCGCGTTTCTGGCATATCCCCgg        >  1:987395/1‑140 (MQ=255)                                                                                                              gTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCTATTTACCATAACGCCTCATTACGCGCACCGCCCAGACTGACTCAGCGCGTTTCTGGCATATCCCCgg        >  2:847039/1‑140 (MQ=255)                                                                                                              gTCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCTATTTACCATAACGCCTCATTACGCGCACCGCCCAGACTGACTCAGCGCGTTTCTGGCATATCCCCgg        >  1:108650/1‑140 (MQ=255)                                                                                                               tCACTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCTATTTACCATAACGCCTCATTACGCGCACCGCCCAGACTGACTCAGCGCGTTTCTGGCATATCCCCGGt       >  1:767518/1‑140 (MQ=255)                                                                                                                 aCTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCTATTTACCATAACGCCTCATTACGCGCACCGCCCAGACTGACTCAGCGCGTTTCTGGCATATc             <  2:1093991/132‑1 (MQ=255)                                                                                                                 aCTTTTGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCTATTTACCATAACGCCTCATTACGCGCACCGCCCAGACTGACTCAGCGCGTTTCTGGCATATc             >  1:1093991/1‑132 (MQ=255)                                                                                                                   ttttGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCTATTTACCATAACGCCTCATTACGCGCACCGCCCAGACTGACTCAGCGCGTTTCTGGCATATCCCCGGTaaaa   >  1:1050283/1‑140 (MQ=255)                                                                                                                    tttGTGGGGGCAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCTATTTACCATAACGCCTCATTACGCGCACCGCCCAGACTGACTCAGCGCGTTTCTGGCATATCCCCGGTAAAAc  <  2:327357/140‑1 (MQ=255)                                                                                                                             |                                                                                                                                   TTAACATTACAAGATATAAGTAATGGACGCACTCCCAATTAGTCTATTTAAATCGCCACGAGTTTAACTGACAACCCATGATCAATTATGAATTGCAACTATTTCTGTAGTCACTTTTGTGGGGACAGTCCACAAAACTGCCAACTTCCGCTTCTTGCTCTTAGCGGACATTAGCATAGGCTATTTACCATAACGCCTCATTACGCGCACCGCCCAGACTGACTCAGCGCGTTTCTGGCATATCCCCGGTAAAAC  >  NC_000913/1646827‑1647081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑