Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,777,381	T→C	G62G (GGT→GGC)	ydiO →	putative acyl‑CoA dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,777,381	0	T	C	100.0%	169.7 / NA	49	G62G (GGT→GGC)	ydiO	putative acyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/28);  total (21/28)

GCGAACTGATTACGACTAACTTTCCGGAAGAGTATTTCCGCACCTGCGATCAAAACGGGACATATCCGCGTGAGTTTATGCGGGCGCTGGCGGATAACGGTATTTCCATGCTTGGCGTGCCGGAAGAATTTGGTGGTATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTCCGGCATTTTTAATTACCAACGGTCAATGTATTCACAGTATGCGCCGTTTCGGTTCTGCAGAGCAGCTACGTAAAA  >  NC_000913/1777245‑1777517                                                                                                                                         |                                                                                                                                         gcgAACTGATTACGACTAACTTTCCGGAAGAGTATTTCCGCACCTGCGATCAAAACGGGACATATCCGCGTGAGTTTATGCGGGCGCTGGCGGATAACGGTATTTCCATGCTTGGCGTGCCGGAAGAATTTGGTGGCATc                                                                                                                                       >  1:491164/1‑140 (MQ=255)     aCTGATTACGACTAACTTTCCGGAAGAGTATTTCCGCACCTGCGATCAAAACGGGACATATCCGCGTGAGTTTATGCGGGCGCTGGCGGATAACGGTATTTCCATGCTTGGCGTGCCGGAAGAATTTGGTGGCATCCCTg                                                                                                                                   <  2:160536/140‑1 (MQ=255)     aCTGATTACGACTAACTTTCCGGAAGAGTATTTCCGCACCTGCGATCAAAACGGGACATATCCGCGTGAGTTTATGCGGGCGCTGGCGGATAACGGTATTTCCATGCTTGGCGTGCCGGAAGAATTTGGTGGCATCCCTg                                                                                                                                   <  2:491164/140‑1 (MQ=255)     aCTGATTACGACTAACTTTCCGGAAGAGTATTTCCACACCTGCGATCAAAACGGGACATATCCGCGTGAGTTTATGCGGGCGCTGGCGGATAACGGTATTTCCATGCTTGGCGTGCCGGAAGAATTTGGTGGCATCCCTg                                                                                                                                   <  1:523893/140‑1 (MQ=255)      cTGATTACGACTAACTTTCCGGAAGAGTATTTCCGCACCTGCGATCAAAACGGGACATATCCGCGTGAGTTTATGCGGGCGCTGGCGGATAACGGTATTTCCATGCTTGGCGTGCCGGAAGAATTTGGTGGCATCCCTGc                                                                                                                                  >  1:607919/1‑140 (MQ=255)              gACTAACTTTCCGGAAGAGTATTTCCGCACCTGCGATCAAAACGGGACATATCCGCGTGAGTTTATGCGGGCGCTGGCGGATAACGGTATTTCCATGCTTGGCGTGCCGGAAGAATTTGGTGGCATCCCTGCGGATTACg                                                                                                                          <  2:1104175/140‑1 (MQ=255)                              gagTATTTCCGCACCTGCGATCAAAACGGGACATATCCGCGTGAGTTTATGCGGGCGCTGGCGGATAACGGTATTTCCATGCTTGGCGTGCCGGAAGAATTTGGTGGCATCCCTGCGGATTACGTCACCCAAATGCTGgc                                                                                                          <  2:819138/140‑1 (MQ=255)                                 tATTTCCGCACCTGCGATCAAAACGGGACATATCCGCGTGAGTTTATGCGGGCGCTGGCGGATAACGGTATTTCCATGCTTGGCGTGCCGGAAGAATTTGGTGGCATCCCTGCGGATTACGTCACCCAAATGCTGGCGCt                                                                                                       >  2:164506/1‑140 (MQ=255)                                     tCCGCACCTGCGATCAAAACGGGACATATCCGCGTGAGTTTATGCGGGCGCTGGCGGATAACGGTATTTCCATGCTTGGCGTGCCGGAAGAATTTGGTGGCATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATg                                                                                                   >  1:35956/1‑140 (MQ=255)                                        gCACCTGCGATCAAAACGGGACATATCCGCGTGAGTTTATGCGGGCGCTGGCGGATAACGGTATTTCCATGCTTGGCGTGCCGGAAGAATTTGGTGGCATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGaa                                                                                                <  2:35956/140‑1 (MQ=255)                                              gCGATCAAAACGGGACATATCCGCGTGAGTTTATGCTGGCGCTGGCGGATAACGGTATTTCCATGCTTGGCGTGCCGGAAGAATTTGGTGGCATCCCTGCGGATTACGTCACCCAAATGCTGgcg                                                                                                         >  1:292022/1‑125 (MQ=255)                                              gCGATCAAAACGGGACATATCCGCGTGAGTTTATGCTGGCGCTGGCGGATAACGGTATTTCCATGCTTGGCGTGCCGGAAGAATTTGGTGGCATCCCTGCGGATTACGTCACCCAAATGCTGgcg                                                                                                         <  2:292022/125‑1 (MQ=255)                                                 aTCAAAACGGGACATATCCGCGTGAGTTTATGCGGGCGCTGGCGGATAACGGTATTTCCATGCTTGGCGTGCCGGAAGAATTTGGTGGCATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCaaaa                                                                                       >  1:878936/1‑140 (MQ=255)                                                   cAAAACGGGACATATCCGCGTGAGTTTATGCGGGCGCTGGCGGATAACGGTATTTCCATGCTTGGCGTGCCGGAAGAATTTGGTGGCATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATg                                                                                     <  2:324635/140‑1 (MQ=255)                                                            aCATATCCGCGTGAGTTTATGCGGGCGCTGGCGGATAACGGTATTTCCATGCTTGGCGTGCCGGAAGAATTTGGTGGCATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTcc                                                                            >  1:47831/1‑140 (MQ=255)                                                                 tCCGCGTGAGTTTATGCGGGCGCTGGCGGATAACGGTATTTCCATGCTTGGCGTGCCGGAAGAATTTGGTGGCATCGCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTCCGGCAt                                                                       <  2:371182/140‑1 (MQ=255)                                                                    gcgTGAGTTTATGCGGGCGCTGGCGGATAACGGTATTTCCATGCTTGGCGTGCCGGAAGAATTTGGTGGCATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTCCGGCAtttt                                                                    <  2:47831/140‑1 (MQ=255)                                                                     cgTGAGTTTATGCGGGCGCTGGCGGATAACGGTATTTCCATGCTTGGCGTGCCGGAAGAATTTGGTGGCATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTCCGGCAttttt                                                                   >  2:29629/1‑140 (MQ=255)                                                                     cgTGAGTTTATGCGGGCGCTGGCGGATAACGGTATTTCCATGCTTGGCGTGCCGGAAGAATTTGGTGGCATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTCCGGCAttttt                                                                   >  1:888171/1‑140 (MQ=255)                                                                       tGAGTTTATGCGGGCGCTGGCGGATAACGGTATTTCCATGCTTGGCGTGCCGGAAGAATTTGGTGGCATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTCCGGCATTTTTaa                                                                 >  2:484832/1‑140 (MQ=255)                                                                             tATGCGGGCGCTGGCGGATAACGGTATTTCCATGCTTGGCGTGCCGGAAGAATTTGGTGGCATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTCCGGCATTTTTAATTACCa                                                           <  1:484832/140‑1 (MQ=255)                                                                                gCGGGCGCTGGCGGATAACGGTATTTCCATGCTTGGCGTGCCGGAAGAATTTGGTGGCATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTCCGGCATTTTTAATTACCAACg                                                        >  2:718905/1‑140 (MQ=255)                                                                                 cGGGCGCTGGCGGATAACGGTATTTCCATGCTTGGCGTGCCGGAAGAATTTGGTGGCATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTCCGGCATTTTTAATTACCAACgg                                                       <  1:164506/140‑1 (MQ=255)                                                                                   ggCGCTGGCGGATAACGGTATTTCCATGCTTGGCGTGCCGGAAGAATTTGGTGGCATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTCCGGCATTTTTAATTACCAACGGTc                                                     <  2:40154/140‑1 (MQ=255)                                                                                   ggCGCTGGCGGATAACGGTATTTCCATGCTTGGCGTGCCGGAAGAATTTGGTGGCATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTCCGGCATTTTTAATTACCAACGGTc                                                     >  1:40154/1‑140 (MQ=255)                                                                                   ggCGCTGGCGGATAACGGTATTTCCATGCTTGGCGTGCCGGAAGAATTTGGTGGCATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTCCGGCATTTTTAATTACCAACGGTc                                                     <  2:888171/140‑1 (MQ=255)                                                                                    gcgcTGGCGGATAACGGTATTTCCATGCTTGGCGTGCCGGAAGAATTTGGTGGCATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTCCGGCATTTTTAATTACCAACGGTCa                                                    <  1:290864/140‑1 (MQ=255)                                                                                    gcgcTGGCGGATAACGGTATTTCCATGCTTGGCGTGCCGGAAGAATTTGGTGGCATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTCCGGCATTTTTAATTACCAACGGTCa                                                    >  2:363657/1‑140 (MQ=255)                                                                                     cgcTGGCGGATAACGGTATTTCCATGCTTGGCGTGCCGGAAGAATTTGGTGGCATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTCCGGCATTTTTAATTACCAACGGTCaa                                                   <  1:926206/140‑1 (MQ=255)                                                                                     cgcTGGCGGATAACGGTATTTCCATGCTTGGCGTGCCGGAAGAATTTGGTGGCATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTCCGGCATTTTTAATTACCAACGGTCaa                                                   >  2:926206/1‑140 (MQ=255)                                                                                      gcTGGCGGATAACGGTATTTCCATGCTTGGCGTGCCGGAAGAATTTGGTGGCATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTCCGGCATTTTTAATTACCAACGGTCAAt                                                  <  1:455349/140‑1 (MQ=255)                                                                                      gcTGGCGGATAACGGTATTTCCATGCTTGGCGTGCCGGAAGAATTTGGTGGCATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTCCGGCATTTTTAATTACCAACGGTCAAt                                                  <  2:878936/140‑1 (MQ=255)                                                                                       cTGGCGGATAACGGTATTTCCATGCTTGGCGTGCCGGAAGAATTTGGTGGCATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTCCGGCATTTTTAATTACCAACGGTCAATg                                                 <  1:211970/140‑1 (MQ=255)                                                                                        tGGCGGATAACGGTATTTCCATGCTTGGCGTGCCGGAAGAATTTGGTGGCATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTCCGGCATTTTTAATTACCAACGGTCAATGt                                                <  2:1058925/140‑1 (MQ=255)                                                                                            ggATAACGGTATTTCCATGCTTGGCGTGCCGGAAGAATTTGGTGGCATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTCCGGCATTTTTAATTACCAACGGTCAATGTATTc                                            <  2:981662/140‑1 (MQ=255)                                                                                             gATAACGGTATTTCCATGCTTGGCGTGCCGGAAGAATTTGGTGGCATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTCCGGCATTTTTAATTACCAACGGTCAATGTATTca                                           <  2:855973/140‑1 (MQ=255)                                                                                                aaCGGTATTTCCATGCTTGGCGTGCCGGAAGAATTTGGTGGCATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTCCGGCATTTTTAATTACCAACGGt                                                      <  1:991643/126‑1 (MQ=255)                                                                                                aaCGGTATTTCCATGCTTGGCGTGCCGGAAGAATTTGGTGGCATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTCCGGCATTTTTAATTACCAACGGt                                                      >  2:991643/1‑126 (MQ=255)                                                                                                    gTATTTCCATGCTTGGCGTGCCGGAAGAATTTGGTGGCATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTCCGGCACTTTTAATTACCAACGGTCaa                                                   >  2:1106518/1‑125 (MQ=255)                                                                                                    gTATTTCCATGCTTGGCGTGCCGGAAGAATTTGGTGGCATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTCCGGCACTTTTAATTACCAACGGTCaa                                                   <  1:1106518/125‑1 (MQ=255)                                                                                                     tATTTCCATGCTTGGCGTGCCGGAAGAATTTGGTGGCATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTCCGGCATTTTTAATTACCAACGGTCAATGTATTCACAGTATgc                                   <  1:363657/140‑1 (MQ=255)                                                                                                       tttCCATGCTTGGCGTGCCGGAAGAATTTGGTGGCATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTCCGGCATTTTTAATTACCAACGGTCAATGTATTCACAGTATgcgc                                 <  2:337788/140‑1 (MQ=255)                                                                                                               cTTGGCGTGCCGGAAGAATTTGGTGGCATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTCCGGCATTTTTAATTACCAACGGTCAATGTATTCACAGTATGCGCCGTTTCgg                         >  1:220803/1‑140 (MQ=255)                                                                                                               cTTGGCGTGCCGGAAGAATTTGGTGGCATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTCCGGCATTTTTAATTACCAACGGTCAATGTATTCACAGTATGCGCCGTTTCgg                         <  2:220803/140‑1 (MQ=255)                                                                                                                  ggCGTGCCGGAAGAATTTGGTGGCATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTCCGGCATTTTTAATTACCAACGGTCAATGTATTCACAGTATGCGCCGTTTCGGTTc                      >  2:848618/1‑140 (MQ=255)                                                                                                                    cGTGCCGGAAGAATTTGGTGGCATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTCCGGCATTTTTAATTACCAACGGTCAATGTATTCACAGTATGCGCCGTTTCGGTTCTg                    <  2:607919/140‑1 (MQ=255)                                                                                                                    cGTGCCGGAAGAATTTGGTGGCATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTCCGGCATTTTTAATTACCAACGGTCAATGTATTCACAGTATGCGCCGTTTCGGTTCTg                    >  2:1110361/1‑140 (MQ=255)                                                                                                                            aagaaTTTGGTGGCATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTCCGGCATTTTTAATTACCAACGGTCAATGTATTCACAGTATGCGCCGTTTCGGTTCTGCAGagcag            >  1:452543/1‑140 (MQ=255)                                                                                                                                   tggtggCATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTCCGGCATTTTTAATTACCAACGGTCAATGTATTCACAGTATGCGCCGTTTCGGTTCTGCAGAGCAGCTACGTa     >  2:589119/1‑140 (MQ=255)                                                                                                                                      tggcATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTCCGGCATTTTTAATTACCAACGGTCAATGTATTCACAGTATGCGCCGTTTCGGTTCTGCAGAGCAGCTACGTaaaa  <  1:718905/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         GCGAACTGATTACGACTAACTTTCCGGAAGAGTATTTCCGCACCTGCGATCAAAACGGGACATATCCGCGTGAGTTTATGCGGGCGCTGGCGGATAACGGTATTTCCATGCTTGGCGTGCCGGAAGAATTTGGTGGTATCCCTGCGGATTACGTCACCCAAATGCTGGCGCTGATGGAAGTGTCAAAATGCGGTGCTCCGGCATTTTTAATTACCAACGGTCAATGTATTCACAGTATGCGCCGTTTCGGTTCTGCAGAGCAGCTACGTAAAA  >  NC_000913/1777245‑1777517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑