Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,949,479	A→C	G348G (GGT→GGG)	aspS ←	aspartate‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,949,479	0	A	C	100.0%	200.6 / NA	58	G348G (GGT→GGG)	aspS	aspartate‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (31/27);  total (31/27)

AATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATGCGCCGCCCGGAACGCGCAGAGCCGCTACGCGACCTTTCGGATCGTTCGCCGGACCTGCAAATACAGCAA  >  NC_000913/1949347‑1949612                                                                                                                                     |                                                                                                                                      aaTCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTcg                                                                                                                                >  2:274757/1‑140 (MQ=255)      tatCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCg                                                                                                                           >  2:29360/1‑140 (MQ=255)       atCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGt                                                                                                                          >  1:678228/1‑140 (MQ=255)       atCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGt                                                                                                                          <  2:410485/140‑1 (MQ=255)       atCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGt                                                                                                                          >  1:246902/1‑140 (MQ=255)           ccATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGAt                                                                                                                      >  2:87759/1‑140 (MQ=255)              tCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTc                                                                                                                                 >  1:729356/1‑126 (MQ=255)              tCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTc                                                                                                                                 <  2:729356/126‑1 (MQ=255)                 tGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAAc                                                                                                                >  1:992612/1‑140 (MQ=255)                  gcgcGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACg                                                                                                               <  1:87759/140‑1 (MQ=255)                  gcgcGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACg                                                                                                               <  2:992612/140‑1 (MQ=255)                  gcgcGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGCTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACg                                                                                                               <  1:375060/140‑1 (MQ=255)                   cgcgGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACGa                                                                                                              >  2:853864/1‑140 (MQ=255)                     cgGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACGAAg                                                                                                            <  1:216732/140‑1 (MQ=255)                        cAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACGAAGTTa                                                                                                         <  2:246902/140‑1 (MQ=255)                           tACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACGAAGt                                                                                                           <  1:479181/135‑1 (MQ=255)                           tACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACGAAGt                                                                                                           >  2:479181/1‑135 (MQ=255)                            aCGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACGAAGTTACCGt                                                                                                     <  2:42955/140‑1 (MQ=255)                                  cAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATtcgt                                                                                               >  2:1047511/1‑140 (MQ=255)                                  cAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATtcgt                                                                                               >  1:965447/1‑140 (MQ=255)                                   aGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATtcgtc                                                                                              <  2:264229/140‑1 (MQ=255)                                   aGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATtcgtc                                                                                              <  1:29360/140‑1 (MQ=255)                                      aTGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGAt                                                                                           <  1:274757/140‑1 (MQ=255)                                         tCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTg                                                                                        >  1:631639/1‑140 (MQ=255)                                          cTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACGAAGTTa                                                                                                         <  1:362124/122‑1 (MQ=255)                                          cTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACGAAGTTa                                                                                                         >  2:362124/1‑122 (MQ=255)                                          cTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGc                                                                                       >  1:969236/1‑140 (MQ=255)                                           ttCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCt                                                                                      >  2:771540/1‑140 (MQ=255)                                            tCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCtt                                                                                     >  1:591510/1‑140 (MQ=255)                                                  atTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGt                                                                               <  2:641454/140‑1 (MQ=255)                                                          aTTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGTGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATg                                                                       <  1:339808/140‑1 (MQ=255)                                                                   cTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGCTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATGCGCCGCCCg                                                              >  2:1113657/1‑140 (MQ=255)                                                                    ttCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATGcgccgc                                                                 >  2:443000/1‑136 (MQ=255)                                                                    ttCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATGcgccgc                                                                 <  1:443000/136‑1 (MQ=255)                                                                      cGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATGCGCCGCCCGGaa                                                           >  1:472814/1‑140 (MQ=255)                                                                        cTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTAGTCGTCGATCTGCTTACGGGTCAGCGATGTGCCGCCCGGAAcg                                                         >  2:1090231/1‑140 (MQ=255)                                                                                cTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATGCGCCGCCCGGAACGCGCAGAGc                                                 <  2:678228/140‑1 (MQ=255)                                                                                        aCCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATGCGCCGCCCGGAACGCGCAGAGCCGCTAcgc                                         <  1:1047511/140‑1 (MQ=255)                                                                                         ccTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATGTGCCGCCCGGAACGCGCAGAGCCGCTAcgcg                                        <  1:1090231/140‑1 (MQ=255)                                                                                         ccTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATGCGCCGCCCGGAACGCGCAGAGCCGCTAcgcg                                        <  2:631639/140‑1 (MQ=255)                                                                                             ccAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATGCGCCGCCCGGAACGCGCAGAGCCGCTAcgcg                                        <  1:117002/136‑1 (MQ=255)                                                                                             ccAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATGCGCCGCCCGGAACGCGCAGAGCCGCTAcgcg                                        <  1:848666/136‑1 (MQ=255)                                                                                             ccAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATGCGCCGCCCGGAACGCGCAGAGCCGCTAcgcg                                        >  2:848666/1‑136 (MQ=255)                                                                                             ccAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATGCGCCGCCCGGAACGCGCAGAGCCGCTAcgcg                                        >  2:117002/1‑136 (MQ=255)                                                                                             ccAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATGCGCCGCCCGGAACGCGCAGAGCCGCTACGCGACCt                                    >  1:259427/1‑140 (MQ=255)                                                                                                gACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATGCGCCGCCCGGAACGCGCAGAGCCGCTAcgc                                         <  1:367818/132‑1 (MQ=255)                                                                                                gACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATGCGCCGCCCGGAACGCGCAGAGCCGCTAcgc                                         >  2:367818/1‑132 (MQ=255)                                                                                                gACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATGCGCCGCCCGGAACGCGCAGAGCCGCTAcg                                          >  2:411806/1‑131 (MQ=255)                                                                                                gACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATGCGCCGCCCGGAACGCGCAGAGCCGCTAcg                                          <  1:411806/131‑1 (MQ=255)                                                                                                 aCCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATGCGCCGCCCGGAACGCGCAGAGCCGCTACGCGACCTTTCg                                >  1:786594/1‑140 (MQ=255)                                                                                                     ttCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATGCGCCGCCCGGAACGCGCAGAGCCGCTACGCGACCTTTCGGATc                            >  2:584019/1‑140 (MQ=255)                                                                                                     ttCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATGCGCCGCCCGGAACGCGCAGAGCCGCTACGCGACCTTTCGGATc                            >  1:492771/1‑140 (MQ=255)                                                                                                      tCGCGCGTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATGCGCCGCCCGGAACGCGCAGAGCCGCTACGCGACCTTTCGGATCg                           >  2:895324/1‑140 (MQ=255)                                                                                                       cgcgcgTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATGCGCCGCCCGGAACGCGCAGAGCCGCTACGCGACCTTTCGGATCGt                          >  2:443081/1‑140 (MQ=255)                                                                                                          gcgTTCGTTAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATGCGCCGCCCGGAACGCGCAGAGCCGCTACGCGACCTTTCGGATCGTTCg                       <  2:591510/140‑1 (MQ=255)                                                                                                           cgttcgttAACTTTGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATGCGCCGCCCGGAACGCGCAGAGCCGCTACGCGACCTTTCGGATCGTTCGc                      <  1:853864/140‑1 (MQ=255)                                                                                                                        tGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATGCGCCGCCCGGAACGCGCAGAGCCGCTACGCGACCTTTCGGATCGTTCGCCGGACCTGCAAAt         <  1:1113657/140‑1 (MQ=255)                                                                                                                        tGATGTAAGCCAGCCCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATGCGCCGCCCGGAACGCGCAGAGCCGCTACGCGACCTTTCGGATCGTTCGCCGGACCTGCAAAt         >  2:1134699/1‑140 (MQ=255)                                                                                                                               agccagccCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATGCGCCGCCCGGAACGCGCAGAGCCGCTACGCGACCTTTCGGATCGTTCGCCGGACCTGCAAATACAGCaa  >  2:902972/1‑140 (MQ=255)                                                                                                                               agccagccCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATGCGCCGCCCGGAACGCGCAGAGCCGCTACGCGACCTTTCGGATCGTTCGCCGGACCTGCAAATACAGCaa  >  2:959339/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                      AATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTCAGCGATGCGCCGCCCGGAACGCGCAGAGCCGCTACGCGACCTTTCGGATCGTTCGCCGGACCTGCAAATACAGCAA  >  NC_000913/1949347‑1949612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑