Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,142,983	A→G	intergenic (‑35/‑283)	udk ← / → dgcE	uridine/cytidine kinase/putative diguanylate cyclase DgcE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,142,983	0	A	G	100.0%	197.4 / NA	57	intergenic (‑35/‑283)	udk/dgcE	uridine/cytidine kinase/putative diguanylate cyclase DgcE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/26);  total (31/26)

CTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATTCCCGGGGGCAAATTACGTTGATCAGTT  >  NC_000913/2142847‑2143114                                                                                                                                         |                                                                                                                                    cTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAgggg                                                                                                                                  >  1:574414/1‑140 (MQ=255)                aTAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAGGGGGGCGACGAAGCGCCa                                                                                                                   <  2:706989/140‑1 (MQ=255)                    aGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAAcg                                                                                                               >  2:959479/1‑140 (MQ=255)                    aGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAAcg                                                                                                               >  1:421543/1‑140 (MQ=255)                      ggTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAAcgcg                                                                                                             >  2:421016/1‑140 (MQ=255)                       gTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAACGCgg                                                                                                            <  2:501071/140‑1 (MQ=255)                       gTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAACGCgg                                                                                                            <  1:614159/140‑1 (MQ=255)                            ggCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAACGCGGCAAtt                                                                                                       >  2:74056/1‑140 (MQ=255)                             gCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAACGCGGCAATta                                                                                                      >  1:411217/1‑140 (MQ=255)                                ctAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGTTGTTAGGAGGGGGGCGACGAAGCGCCAAACGCGGCAATTATAg                                                                                                   >  2:838728/2‑140 (MQ=255)                                aTAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAACGCgg                                                                                                            <  1:380374/131‑1 (MQ=255)                                aTAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAACGCgg                                                                                                            <  1:549481/131‑1 (MQ=255)                                aTAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAACGCgg                                                                                                            >  2:380374/1‑131 (MQ=255)                                aTAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAACGCgg                                                                                                            >  2:549481/1‑131 (MQ=255)                                aTAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAACGCGGCAATTATAg                                                                                                   >  1:199028/1‑140 (MQ=255)                                   aGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGa                                                                                                <  1:456541/140‑1 (MQ=255)                                       tctTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAGGGGGGCGATGAAGCGCCAAACGCGGCAATTATAGGGATTTc                                                                                            >  2:1014682/1‑140 (MQ=255)                                       tctTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTc                                                                                            >  2:230277/1‑140 (MQ=255)                                         ttGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCAt                                                                                          <  1:197989/140‑1 (MQ=255)                                         ttGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCAt                                                                                          <  2:335462/140‑1 (MQ=255)                                                  gCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGAt                                                                                 <  2:170702/140‑1 (MQ=255)                                                    cGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATAc                                                                               <  1:230277/140‑1 (MQ=255)                                                     gATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATAcc                                                                              <  2:574414/140‑1 (MQ=255)                                                      aTGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCa                                                                             >  1:1143859/1‑140 (MQ=255)                                                             aGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAAt                                                                      >  2:291300/1‑140 (MQ=255)                                                                   aCCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGtt                                                                >  2:402741/1‑140 (MQ=255)                                                                      gATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGcc                                                             >  2:175744/1‑140 (MQ=255)                                                                             aCGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCt                                                      <  1:175744/140‑1 (MQ=255)                                                                             aCGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCt                                                      <  1:1014682/140‑1 (MQ=255)                                                                             aCGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCt                                                      <  2:1143859/140‑1 (MQ=255)                                                                                cACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCag                                                   >  1:460745/1‑140 (MQ=255)                                                                                 aCTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCaga                                                  <  2:411217/140‑1 (MQ=255)                                                                                   tgatgaGACTGATCAGTCATATATTTAGCGACCTGATTAATCTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAACG‑GGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCcg                                                            >  1:156190/1‑127 (MQ=255)                                                                                   tgatgaGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAGGGGGGCGACGAAGCGCCAATCG‑GGCAATTATAGGGATTTCATCCGTCTGATACCAGTTGAATAGCGTTGCcg                                                            <  2:156190/127‑1 (MQ=255)                                                                                      tgaGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTaa                                             >  2:211697/1‑140 (MQ=255)                                                                                             gATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGa                                      <  2:460745/140‑1 (MQ=255)                                                                                              aTCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGAc                                     >  2:874809/1‑140 (MQ=255)                                                                                               tCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACa                                    >  1:1051650/1‑140 (MQ=255)                                                                                                      tatatTTAGCGACCTGATTAACCTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAAtt                             >  1:152557/1‑140 (MQ=255)                                                                                                        tatTTAGCGACCTGATTAACCTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATTcc                           >  1:15915/1‑140 (MQ=255)                                                                                                        tatTTAGCGACCTGATTAACCTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATTcc                           >  1:620601/1‑140 (MQ=255)                                                                                                          tttAGCGACCTGATTAACCTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATTCCCg                         >  2:437658/1‑140 (MQ=255)                                                                                                             aGCGACCTGATTAACCTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATTCCCgggg                      >  1:939308/1‑140 (MQ=255)                                                                                                             aGCGACCTGATTAACCTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATTCCCgggg                      <  2:421543/140‑1 (MQ=255)                                                                                                               cGACCTGATTAACCTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATTCCCGGGGGc                    >  2:316084/1‑140 (MQ=255)                                                                                                               cGACCTGATTAACCTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATTCCCGGGGGc                    >  1:1068079/1‑140 (MQ=255)                                                                                                               cGACCTGATTAACCTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATTCCCGGGGGc                    >  1:142852/1‑140 (MQ=255)                                                                                                                gACCTGATTAACCTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATTCCCGGGGGCa                   <  2:1051650/140‑1 (MQ=255)                                                                                                                   cTGATTAACCTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATTCCCGGGGGCAAAt                >  2:1136798/1‑140 (MQ=255)                                                                                                                            cTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATTCCCGGGGGCAAATTACGTTGAt       <  1:402741/140‑1 (MQ=255)                                                                                                                            cTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATTCCCGGGGGCAAATTACGTTGAt       <  1:291300/140‑1 (MQ=255)                                                                                                                            cTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATTCCCGGGGGCAAATTACGTTGAt       <  1:421016/140‑1 (MQ=255)                                                                                                                            cTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATTCCCGGGGGCAAATTACGTTGAt       <  2:413325/140‑1 (MQ=255)                                                                                                                            cTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATTCCCGGGGGCAAATTACGTTGAt       <  1:74056/140‑1 (MQ=255)                                                                                                                            cTGGATGTTAGGAGGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATTCCCGGGGGCAAATTACGTTGAt       <  2:152557/140‑1 (MQ=255)                                                                                                                                aTGTTAGGAGGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATTCCCGGGGGCAAATTACGTTGATCAGt   >  1:236900/1‑140 (MQ=255)                                                                                                                                aTGTTAGGAGGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATTCCCGGGGGCAAATTACGTTGATCAGt   >  1:15902/1‑140 (MQ=255)                                                                                                                                aTGTTAGGAGGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATTCCCGGGGGCAAATTACGTTGATCAGt   >  2:188458/1‑140 (MQ=255)                                                                                                                                 tGTTAGGAGGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATTCCCGGGGGCAAATTACGTTGATCAGtt  <  1:188458/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    CTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATTCCCGGGGGCAAATTACGTTGATCAGTT  >  NC_000913/2142847‑2143114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑