| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,167,184 | A→G | noncoding (71/87 nt) | cyaR → | small regulatory RNA CyaR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,167,184 | 0 | A | G | 97.9% | 160.7 / ‑4.4 | 48 | noncoding (71/87 nt) | cyaR | small regulatory RNA CyaR |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (27/20); total (28/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
ATGGAAAATTCTTAGAAACCGATCACATACAGCTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGC > NC_000913/2167053‑2167320 | aTGGAAAATTCTTAGAAACCGATCACATACAGCTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCt > 1:899731/1‑140 (MQ=255) ttAGAAACCGATCACATACAGCTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTGCACGGGCTTATTTTTTAcg > 2:528984/1‑140 (MQ=255) aGAAACCGATCACATACAGCTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTGCACGGGCTTATTTTTTAcgcg < 1:989853/140‑1 (MQ=255) aaCCGATCACATACAGCTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTGCACGGGCTTATTTTTTACGCGTaa < 1:95248/140‑1 (MQ=255) cGATCACATACAGCTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTGCACGGGCTTATTTTTTAcgcg > 2:196104/1‑134 (MQ=255) cGATCACATACAGCTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTGCACGGGCTTATTTTTTAcgcg < 1:196104/134‑1 (MQ=255) cTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTGCACGGGCTTATTTTTTACGCGTAATACaa < 2:483265/129‑1 (MQ=255) cTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTGCACGGGCTTATTTTTTACGCGTAATACaa > 1:483265/1‑129 (MQ=255) cTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTGCACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAg > 2:152930/1‑140 (MQ=255) cTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTGCACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAg > 1:961277/1‑140 (MQ=255) cTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTGCACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAg > 1:949101/1‑140 (MQ=255) cTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTGCACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAg > 1:695731/1‑140 (MQ=255) aTTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTGCACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGatt > 1:1035379/1‑140 (MQ=255) aTTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTGCA‑‑‑‑CTTATTTTTTACGCGTAATACAATGAAATAAAAGGatttatt > 2:653268/1‑140 (MQ=255) attaAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTGCACGGGCTTATTTTTTACGCGTAATACAATGAAATaaaa < 1:95956/131‑1 (MQ=255) attaAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTGCACGGGCTTATTTTTTACGCGTAATACAATGAAATaaaa > 2:95956/1‑131 (MQ=255) ttaAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTGCACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGatttattt < 1:364995/140‑1 (MQ=255) tATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTGCACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCa < 2:1131839/140‑1 (MQ=255) atcatcCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTGCACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCAc > 1:945822/1‑140 (MQ=255) atcatcCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTGCACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCAc > 2:198048/1‑140 (MQ=255) atcCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTGCACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTc < 2:811154/140‑1 (MQ=255) gTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTGCACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCcaca > 2:355948/1‑140 (MQ=255) cTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTGCACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGa > 1:11108/1‑140 (MQ=255) aaaaCATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTGCACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACcac < 2:1035379/140‑1 (MQ=255) cATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTGCACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCg > 1:805060/1‑140 (MQ=255) tGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTGCACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCt > 2:919772/1‑140 (MQ=255) gCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTGCACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTc < 1:919772/140‑1 (MQ=255) gCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTGCACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTc < 1:1061958/140‑1 (MQ=255) cTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTGCACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTg > 1:900251/1‑140 (MQ=255) gTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTGCACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAg < 2:562571/140‑1 (MQ=255) tACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTGCACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAgg > 1:131308/1‑140 (MQ=255) aCCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTGCACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAggg > 1:578765/1‑140 (MQ=255) ccAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTGCACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAgggg > 2:101070/1‑140 (MQ=255) cAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTGCACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGc < 2:900251/140‑1 (MQ=255) gAACCACCTCCTTAGCCTGTGTAATCTCCCTTGCACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAg < 2:300502/133‑1 (MQ=255) gAACCACCTCCTTAGCCTGTGTAATCTCCCTTGCACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAg > 1:300502/1‑133 (MQ=255) gAACCACCTCCTTAGCCTGTGTAATCTCCCTTGCACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTc < 2:945822/140‑1 (MQ=255) accaccTCCTTAGCCTGTGTAATCTCCCTTGCACGGGCTTATTTTTTACGCGTAATACAATGAAGTAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCt > 2:254707/1‑140 (MQ=255) accaccTCCTTAGCCTGTGTAATCTCCCTTGCACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACt < 1:1042652/128‑1 (MQ=255) accaccTCCTTAGCCTGTGTAATCTCCCTTGCACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACt > 2:1042652/1‑128 (MQ=255) accaccTCCTTAGCCTGTGTAATCTCCCTTGCACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCt < 1:1129643/140‑1 (MQ=255) caccTCCTTAGCCTGTGTAATCTCCCTTGCACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGATAAAAAAGCCCCTCGACTGAGGGGCTTCCTGt > 2:940044/1‑140 (MQ=255) caccTCCTTAGCCTGTGTAATCTCCCTTGCACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGt > 2:951533/1‑140 (MQ=255) cctcctTAGCCTGTGTAATCTCCCTTGCACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGttt < 1:528984/140‑1 (MQ=255) ttAGCCTGTGTAATCTCCCTTGCACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAAt > 2:1035822/1‑140 (MQ=255) ccTGTGTAATCTCCCTTGCACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACa < 2:344158/140‑1 (MQ=255) gtAATCTCCCTTGCACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCcac > 1:522925/1‑140 (MQ=255) cTTGCACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTtgc < 2:961277/140‑1 (MQ=255) | ATGGAAAATTCTTAGAAACCGATCACATACAGCTGCATTTATTAAGGTTATCATCCGTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGC > NC_000913/2167053‑2167320 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |