Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,264,383	T→C	V174A (GTA→GCA)	setB →	sugar exporter SetB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,264,383	0	T	C	100.0%	118.3 / NA	36	V174A (GTA→GCA)	setB	sugar exporter SetB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/16);  total (20/16)

TGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGTAATGTATCTGAGCGCAGCGGTAGCGTTTATTGTTTGCGGTGTGATGGTGTGGCTGTTTTTACCGTCGATGCGAAAAGAGCTTCCGCTGGCGACCGGCACGATCGAAGCGCCGCGCCGTAACCGTCGCGATACGC  >  NC_000913/2264249‑2264517                                                                                                                                       |                                                                                                                                       tgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGCAATGt                                                                                                                                   >  1:860127/1‑140 (MQ=255)  gcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGCAATGTa                                                                                                                                  >  1:343245/1‑140 (MQ=255)     cGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGCAATGTATCt                                                                                                                               <  2:860127/140‑1 (MQ=255)       tGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGCaa                                                                                                                                      >  2:160895/1‑131 (MQ=255)            aTGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGCAATGTATCTGAGCGCa                                                                                                                        >  2:129650/1‑140 (MQ=255)              gCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGCAATGTATCTGAGCGCAGc                                                                                                                      >  1:1150565/1‑140 (MQ=255)                   cAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGCAATGTATCTGAGCGCAGCGGTAg                                                                                                                 <  1:864467/140‑1 (MQ=255)                          ggACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGCAATGTATCTGAGCGCAGCGGTAGCGTTTAt                                                                                                          <  1:129650/140‑1 (MQ=255)                              gTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGCAATGTATCTGAGCGCAGCGGTAGCGTTTATTGtt                                                                                                      <  1:351959/140‑1 (MQ=255)                                gAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGCAATGTATCTGAGCGCAGCGGTAGCGTTTATTGTTTg                                                                                                    <  2:466481/140‑1 (MQ=255)                                  ggCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGCAATGTATCTGAGCGCAGCGGTAGCGTTTATTGTTTGCg                                                                                                  <  1:350061/140‑1 (MQ=255)                                     ggTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGCAATGTATCTGAGCGCAGCGGTAGCG‑TTATTGTTTGCGgtgt                                                                                              <  2:581675/140‑1 (MQ=255)                                              cAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGCAATGTATCTGAGCGCAGCGGTAGCGTTTATTGTTTg                                                                                                    <  2:850254/126‑1 (MQ=255)                                              cAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGCAATGTATCTGAGCGCAGCGGTAGCGTTTATTGTTTg                                                                                                    >  1:850254/1‑126 (MQ=255)                                                  tctTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGCAATGTATCTGAGCGCAGCGGTAGCGTTTATTGTTTGCGGTGTGATGGTGTGGCt                                                                                  <  2:343245/140‑1 (MQ=255)                                                                                  ggTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGCAATGTATCTGAGCGCAGCGGTAGCGTTTATTGTTTGCGGTGTGATGGTGTGGCTGTTTTTACCGTCGATGCGAAAAGAGCTTCCGc                                                  >  1:882005/1‑140 (MQ=255)                                                                                   gTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGCAATGTATCTGAGCGCAGCGGTAGCGTTTATTGTTTGCGGTGTGATGGTGTGGCTGTTTTTACCGTCGATGCGAAAAGAGCTTCCGCt                                                 <  1:671364/140‑1 (MQ=255)                                                                                       ttGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGCAATGTATCTGAGCGCAGCGGTAGCGTTTATTGTTTGCGGTGTGATGGTGTGGCTGTTTTTACCGTCGATGCGAAAAGAGCTTCCg                                                   >  1:1015492/1‑134 (MQ=255)                                                                                       ttGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGCAATGTATCTGAGCGCAGCGGTAGCGTTTATTGTTTGCGGTGTGATGGTGTGGCTGTTTTTACCGTCGATGCGAAAAGAGCTTCCg                                                   <  2:1015492/134‑1 (MQ=255)                                                                                          gCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGCAATGTATCTGAGCGCAGCGGTAGCGTTTATTGTTTGCGGTGTGATGGTGTGGCTGTTTTTACCGTCGATGCGAAAAGAGCTTCCGCTGGCGAcc                                          <  2:1150565/140‑1 (MQ=255)                                                                                            ccACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGCAATGTATCTGAGCGCAGCGGTAGCGTTTATTGTTTGCGGTGTGATGGTGTGGCTGTTTTTACCGTCGATGCGAAAAGAGCTTCCGCTGGCGACCgg                                        <  1:506113/140‑1 (MQ=255)                                                                                              aCCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGCAATGTATCTGAGCGCAGCGGTAGCGTTTATTGTTTGCGGTGTGATGGTGTGGCTGTTTTTACCGTCGATGCGAAAAGAGCTTCCGCTGGCGACCGGCa                                      >  1:104537/1‑140 (MQ=255)                                                                                                  cTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGCAATGTATCTGAGCGCAGCGGTAGCGTTTATTGTTTGCGGTGTGATGGTGTGGCTGTTTTTACCGTCGATGCGAAAAGAGCTTCCGCTGGCGACCGGCACGAt                                  >  1:6907/1‑140 (MQ=255)                                                                                                     gCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGCAATGTATCTGAGCGCAGCGGTAGCGTTTATTGTTTGCGGTGTGATGGTGTGGCTGTTTTTACCGTCGATGCGAAAAGAGCTTCCGCTGGCGACCGGCACGATCGa                               >  2:344911/1‑140 (MQ=255)                                                                                                          tGCCTTAGCGATGGGTTTCAGCTTTACGGCAATGTATCTGAGCGCAGCGGTAGCGTTTATTGTTTGCGGTGTGATGGTGTGGCTGTTTTTACCGTCGATGCGAAAAGAGCTTCCGCTGGCGACCGGCACGATCGAAgcgc                          >  1:191052/1‑140 (MQ=255)                                                                                                            ccTTAGCGATGGGTTTCAGCTTTACGGCAATGTATCTGAGCGCAGCGGTAGCGTTTATTGTTTGCGGTGTGATGGTGTGGCTGTTTTTACCGTCGATGCGAAAAGAGCTTCCGCTGGCGACCGGCACGATCGAAgcgccg                        <  2:882005/140‑1 (MQ=255)                                                                                                            ccTTAGCGATGGGTTTCAGCTTTACGGCAATGTATCTGAGCGCAGCGGTAGCGTTTATTGTTTGCGGTGTGATGGTGTGGCTGTTTTTACCGTCGATGCGAAAAGAGCTTCCGCTGGCGACCGGCACGATCGAAgcgccg                        <  2:191052/140‑1 (MQ=255)                                                                                                               tAGCGATGGGTTTCAGCTTTACGGCAATGTATCTGAGCGCAGCGGTAGCGTTTATTGTTTGCGGTGTGATGGTGTGGCTGTTTTTACCGTCGATGCGAAAAGAGCTTCCGCTGGCGACCGGCACGATCGAAgcgccgcgc                     >  1:1062125/1‑140 (MQ=255)                                                                                                               tAGCGATGGGTTTCAGCTTTACGGCAATGTATCTGAGCGCAGCGGTAGCGTTTATTGTTTGCGGTGTGATGGTGTGGCTG‑TTTTACCGTCGATGCGAAAAGAGCTTCCGCTGGCGACCGGCACGATCGAAgcgccgcgcc                    >  1:24325/1‑140 (MQ=255)                                                                                                                     tGGGTTTCAGCTTTACGGCAATGTATCTGAGCGCAGCGGTAGCGTTTATTGTTTGCGGTGTGATGGTGTGGCTGTTTTTACCGTCGATGCGAAAAGAGCTTCCGCTGGCGACCGGCACGATCGAAGCGCCGCGCCGTAAc               >  2:874795/1‑140 (MQ=255)                                                                                                                      gggTTTCAGCTTTACGGCAATGTATCTGAGCGCAGCGGTAGCGTTTATTGTTTGCGGTGTGATGGTGTGGCTGTTTTTACCGTCGATGCGAAAAGAGCTTCCGCTGGCGACCGGCACGATCGAAGCGCCGCGCCGTAAcc              >  1:490174/1‑140 (MQ=255)                                                                                                                       ggTTTCAGCTTTACGGCAATGTATCTGAGCGCAGCGGTAGCGTTTATTGTTTGCGGTGTGATGGTGTGGCTGTTTTTACCGTCGATGCGAAAAGAGCTTCCGCTGGCGACCGGCACGATCGAAGCGCCGCGCCGTAACCg             <  1:3401/140‑1 (MQ=255)                                                                                                                       ggTTTCAGCTTTACGGCAATGTATCTGAGCGCAGCGGTAGCGTTTATTGTTTGCGGTGTGATGGTGTGGCTGTTTTTACCGTCGATGCGAAAAGAGCTTCCGCTGGCGACCGGCACGATCGAAGCGCCGCGCCGTAACCg             >  2:3401/1‑140 (MQ=255)                                                                                                                                  tACGGCAATGTATCTGAGCGCAGCGGTAGCGTTTATTGTTTGCGGTGTGATGGTGTGGCTGTTTTTACCGTCGATGCGAAAAGAGCTTCCGCTGGCGACCGGCACGATCGAAGCGCCGCGCCGTAACCGTCGCGATACgc  >  2:948278/1‑140 (MQ=255)                                                                                                                                  tACGGCAATGTATCTGAGCGCAGCGGTAGCGTTTATTGTTTGCGGTGTGATGGTGTGGCTGTTTTTACCGTCGATGCGAAAAGAGCTTCCGCTGGCGACCGGCACGATCGAAGCGCCGCGCCGTAACCGTCGCGATACgc  >  1:521558/1‑140 (MQ=255)                                                                                                                                  tACGGCAATGTATCTGAGCGCAGCGGTAGCGTTTATTGTTTGCGGTGTGATGGTGTGGCTGTTTTTACCGTCGATGCGAAAAGAGCTTCCGCTGGCGACCGGCACGATCGAAGCGCCGCGCCGTAACCGTCGCGATACgc  >  1:1027495/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                       TGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCTTATGCCTTAGCGATGGGTTTCAGCTTTACGGTAATGTATCTGAGCGCAGCGGTAGCGTTTATTGTTTGCGGTGTGATGGTGTGGCTGTTTTTACCGTCGATGCGAAAAGAGCTTCCGCTGGCGACCGGCACGATCGAAGCGCCGCGCCGTAACCGTCGCGATACGC  >  NC_000913/2264249‑2264517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑