Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,276,396	G→A	E33K (GAG→AAG)	yejF →	putative oligopeptide ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,276,396	0	G	A	100.0%	261.7 / NA	74	E33K (GAG→AAG)	yejF	putative oligopeptide ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (44/30);  total (44/30)

CCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCGC  >  NC_000913/2276265‑2276531                                                                                                                                    |                                                                                                                                        ccGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGc                                                                                                                                 >  2:123996/1‑140 (MQ=255)  cgcgACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCg                                                                                                                                >  2:969904/1‑140 (MQ=255)          aTTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAAcgctg                                                                                                                        >  1:384553/1‑140 (MQ=255)           tttGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAAcgctgg                                                                                                                       <  1:172198/140‑1 (MQ=255)                      taaGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTggtgggt                                                                                                            <  2:233366/140‑1 (MQ=255)                      taaGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTggtgggt                                                                                                            >  2:582872/1‑140 (MQ=255)                         ggCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAg                                                                                                         >  2:476788/1‑140 (MQ=255)                          gCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGt                                                                                                        >  1:1039715/1‑140 (MQ=255)                              tgtAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGt                                                                                                        <  1:238103/136‑1 (MQ=255)                              tgtAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGt                                                                                                        >  2:238103/1‑136 (MQ=255)                              tgtAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAgg                                                                                                    >  1:973620/1‑140 (MQ=255)                              tgtAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAgg                                                                                                    >  2:466525/1‑140 (MQ=255)                                     tGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGc                                                                                             <  2:684349/140‑1 (MQ=255)                                     tGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGCCAGGTTCAGGc                                                                                             >  1:534674/1‑140 (MQ=255)                                        cGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGt                                                                                                        <  1:257283/126‑1 (MQ=255)                                        cGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGt                                                                                                        >  2:257283/1‑126 (MQ=255)                                        cGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCaaa                                                                                          >  2:816752/1‑140 (MQ=255)                                        cGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCaaa                                                                                          >  2:1110510/1‑140 (MQ=255)                                        cGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCaaa                                                                                          >  2:312044/1‑140 (MQ=255)                                        cGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCaaa                                                                                          >  1:62621/1‑140 (MQ=255)                                        cGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGAAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGGTCAGGCaaa                                                                                          >  2:700512/1‑140 (MQ=255)                                          cAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAg                                                                                        >  1:384945/1‑140 (MQ=255)                                             aCTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGt                                                                                     >  1:910781/1‑140 (MQ=255)                                                   ttAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACcgc                                                                               <  2:900612/140‑1 (MQ=255)                                                    tAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACcgcg                                                                              <  1:582872/140‑1 (MQ=255)                                                    tAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACcgcg                                                                              <  2:560606/140‑1 (MQ=255)                                                    tAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACcgcg                                                                              <  2:910781/140‑1 (MQ=255)                                                         aTTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACcgcg                                                                              >  1:1013331/1‑135 (MQ=255)                                                         aTTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACcgcg                                                                              <  2:1013331/135‑1 (MQ=255)                                                         aTTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTc                                                                         >  2:80312/1‑140 (MQ=255)                                                           tGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACcg                                                                                <  2:475141/131‑1 (MQ=255)                                                           tGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACcg                                                                                >  1:475141/1‑131 (MQ=255)                                                           tGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCaa                                                                       >  2:310725/1‑140 (MQ=255)                                                           tGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCaa                                                                       >  2:615212/1‑140 (MQ=255)                                                             aaaaTTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAAtt                                                                     >  2:783312/1‑140 (MQ=255)                                                                    gTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACcgcg                                                                              <  2:992512/124‑1 (MQ=255)                                                                    gTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACcgcg                                                                              >  1:992512/1‑124 (MQ=255)                                                                      cGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTATGCCTg                                                            <  1:783312/140‑1 (MQ=255)                                                                                tCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTccc                                                  <  1:80312/140‑1 (MQ=255)                                                                                 cGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTcccc                                                 <  2:849861/140‑1 (MQ=255)                                                                                   ccATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCccgc                                               <  1:123996/140‑1 (MQ=255)                                                                                   ccATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCccgc                                               <  2:1039715/140‑1 (MQ=255)                                                                                      tCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCgg                                            <  1:312044/140‑1 (MQ=255)                                                                                        aGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGtt                                          <  2:876805/140‑1 (MQ=255)                                                                                          cAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGa                                        >  1:662726/1‑140 (MQ=255)                                                                                           aaaCCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGaa                                       <  2:662726/140‑1 (MQ=255)                                                                                              ccGTACGTACAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAatat                                    <  1:508333/140‑1 (MQ=255)                                                                                                 tacgtacAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATAtctc                                 >  2:313708/1‑140 (MQ=255)                                                                                                 tacgtacAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATAtctc                                 >  2:422626/1‑140 (MQ=255)                                                                                                    gtacAGTAGTCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTcc                              >  2:931446/1‑140 (MQ=255)                                                                                                         gtagtCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGa                         >  1:457503/1‑140 (MQ=255)                                                                                                          tagtCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGat                        >  2:742686/1‑140 (MQ=255)                                                                                                           agtCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGata                       >  2:480453/1‑140 (MQ=255)                                                                                                            gtCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGatat                      >  1:515450/1‑140 (MQ=255)                                                                                                             tCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATAtt                     >  2:1092643/1‑140 (MQ=255)                                                                                                             tCAATGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATAtt                     >  1:491760/1‑140 (MQ=255)                                                                                                               aaTGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCg                   <  2:491760/140‑1 (MQ=255)                                                                                                               aaTGATGTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCg                   >  1:540384/1‑140 (MQ=255)                                                                                                                 tgatgTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGtt                 >  1:47776/1‑140 (MQ=255)                                                                                                                 tgatgTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGtt                 >  2:928425/1‑140 (MQ=255)                                                                                                                  gatgTTTCACTACAGATTAAGGCTGGCGCAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGttt                >  1:907621/1‑140 (MQ=255)                                                                                                                  gatgTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGttt                <  1:310725/140‑1 (MQ=255)                                                                                                                    tgTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTc              >  1:610571/1‑140 (MQ=255)                                                                                                                    tgTTTCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTc              >  1:616956/1‑140 (MQ=255)                                                                                                                       ttCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGa                         >  2:136556/1‑126 (MQ=255)                                                                                                                       ttCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGa                         <  1:136556/126‑1 (MQ=255)                                                                                                                       ttCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATAtt                     <  1:8520/130‑1 (MQ=255)                                                                                                                       ttCACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATAtt                     >  2:8520/1‑130 (MQ=255)                                                                                                                         cACTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGc         >  2:771208/1‑140 (MQ=255)                                                                                                                          aCTACAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCg        <  2:1060515/140‑1 (MQ=255)                                                                                                                             aCAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAAt     <  1:771208/140‑1 (MQ=255)                                                                                                                              cAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATAtt                     >  2:13272/1‑123 (MQ=255)                                                                                                                              cAGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATAtt                     <  1:13272/123‑1 (MQ=255)                                                                                                                               aGATTAAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCg   <  1:969904/140‑1 (MQ=255)                                                                                                                                gATTAAGGCTGGCGAAGCGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCgc  <  2:907621/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                        CCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCGC  >  NC_000913/2276265‑2276531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑