Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,356,477	G→A	G319G (GGG→GGA)	glpC →	anaerobic glycerol‑3‑phosphate dehydrogenase subunit C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,356,477	0	G	A	100.0%	226.2 / NA	64	G319G (GGG→GGA)	glpC	anaerobic glycerol‑3‑phosphate dehydrogenase subunit C
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/36);  total (28/36)

CAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGGCTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCAAAAAAGAGAACTACCCCACCTCACAAGCCATCGGCGCACCACTGTTCCGCCAGATAGAAGAAAGCGGCGCAGATCTGG  >  NC_000913/2356348‑2356613                                                                                                                                  |                                                                                                                                         cAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGACTTGAGTTaa                                                                                                                                <  2:838388/140‑1 (MQ=255)     cTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGt                                                                                                                            <  1:603321/140‑1 (MQ=255)     cTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGt                                                                                                                            <  1:378120/140‑1 (MQ=255)        gACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTg                                                                                                                           >  1:478868/1‑138 (MQ=255)        gACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTg                                                                                                                           <  2:478868/138‑1 (MQ=255)              ggCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTg                                                                                                                        <  2:334383/135‑1 (MQ=255)              ggCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTg                                                                                                                        >  1:334383/1‑135 (MQ=255)              ggCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTc                                                                                                                   >  1:1057997/1‑140 (MQ=255)                cAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTccc                                                                                                                 <  1:1102730/140‑1 (MQ=255)                cAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTccc                                                                                                                 <  2:502290/140‑1 (MQ=255)                  aaaCGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAg                                                                                                               <  2:748772/140‑1 (MQ=255)                    aCGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGtg                                                                                                             <  1:45722/140‑1 (MQ=255)                             cTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTAt                                                                                                    >  1:867258/1‑140 (MQ=255)                                  aCCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACA‑CCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCggg                                                                                              >  1:740287/1‑140 (MQ=255)                                        gccgctgAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACtt                                                                                         >  2:45262/1‑140 (MQ=255)                                                      tttATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTTCTGCGGTATTGCGGGTACTTACGGTTTCaaaaaa                                                                           <  2:575532/140‑1 (MQ=255)                                                      tttATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCaaaaaa                                                                           <  1:448295/140‑1 (MQ=255)                                                      tttATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCaaaaaa                                                                           <  2:32402/140‑1 (MQ=255)                                                      tttATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCaaaaaa                                                                           <  1:818768/140‑1 (MQ=255)                                                        tATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACA‑CCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCAAAAAagag                                                                        <  2:740287/140‑1 (MQ=255)                                                            acacTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCAAAAAAGAGAAc                                                                     <  2:867258/140‑1 (MQ=255)                                                               cTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCAAAAAAGAGAACTAc                                                                  >  1:915621/1‑140 (MQ=255)                                                               cTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCAAAAAAGAGAACTAc                                                                  >  2:5962/1‑140 (MQ=255)                                                                 ccGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCAAAAAAGAGAACTAccc                                                                <  1:623012/140‑1 (MQ=255)                                                                  cGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCAAAAAAGAGAACTAcccc                                                               >  2:847341/1‑140 (MQ=255)                                                                  cGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCAAAAAAGAGAACTAcccc                                                               >  2:882812/1‑140 (MQ=255)                                                                   gTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCAAAAAAGAGAACTACCCCa                                                              <  1:302424/140‑1 (MQ=255)                                                                    tGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCAAAAAAGAGAACTAcccc                                                               >  1:245921/1‑138 (MQ=255)                                                                    tGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCAAAAAAGAGAACTAcccc                                                               <  2:245921/138‑1 (MQ=255)                                                                    tGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCAAAAAAGAGAACTACCCCAc                                                             >  2:394697/1‑140 (MQ=255)                                                                    tGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCAAAAAAGAGAACTACCCCAc                                                             >  2:1634/1‑140 (MQ=255)                                                                            ggAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCAAAAAAGAGAACTACCCCACCTCACAAg                                                     >  2:965657/1‑140 (MQ=255)                                                                              aaaaaaTGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCAAAAAAGAGAACTACCCCACCTCACAAGcc                                                   >  2:864714/1‑140 (MQ=255)                                                                                aaaaTGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCGAAAAAGAGAACTACCCCACCTCACAAGCCAt                                                 <  1:1102966/140‑1 (MQ=255)                                                                                aaaaTGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCAAAAAAGAGAACTACCCCACCTCACAAGCCAt                                                 <  1:955316/140‑1 (MQ=255)                                                                                   aTGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCAAAAAAGAGAACTACCCCACCTCACAAGCCATCgg                                              <  1:394697/140‑1 (MQ=255)                                                                                     gggCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCAAAAAAGAGAACTACCCCACCTCACAAGCCATCGgcg                                            <  2:249598/140‑1 (MQ=255)                                                                                        cTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCAAAAAAGAGAACTACCCCACCTCACAAGCCATCGGCGcac                                         <  1:965657/140‑1 (MQ=255)                                                                                         tGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCAAAAAAGAGAACTACCCCACCTCACAAGCCATCGGCGcacc                                        <  1:847341/140‑1 (MQ=255)                                                                                            aCGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCAAAAAAGAGAACTACCCCACCTCACAAGCCATCGGCGCACCACt                                     >  2:29774/1‑140 (MQ=255)                                                                                             cGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCAAAAAAGAGAACTACCCCACCTCACAAGCCATCGGCGCACCACTg                                    <  2:1057997/140‑1 (MQ=255)                                                                                             cGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCAAAAAAGAGAACTACCCCACCTCACAAGCCATCGGCGCACCACTg                                    <  1:882812/140‑1 (MQ=255)                                                                                                tctACACCCTGGAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCAAAAAAGAGAACTACCCCACCTCACAAGCCATCGGCGCACCACTGTTc                                 <  1:122085/140‑1 (MQ=255)                                                                                                     acCCTGGAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCAAAAAAGAGAACTACCCCACCTCACAAGCCATCGGCGCACCACTGTTCCGCCa                            >  2:198978/1‑140 (MQ=255)                                                                                                     acCCTGGAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCAAAAAAGAGAACTACCCCACCTCACAAGCCATCGGCGCACCACTGTTCCGCCa                            >  2:211341/1‑140 (MQ=255)                                                                                                        cTGGAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCAAAAAAGAGAACTACCCCACCTCACAAGCCATCGGCGCACCACTGTTCCGcc                             <  2:357260/136‑1 (MQ=255)                                                                                                        cTGGAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCAAAAAAGAGAACTACCCCACCTCACAAGCCATCGGCGCACCACTGTTCCGcc                             >  1:357260/1‑136 (MQ=255)                                                                                                          ggAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCAAAAAAGAGAACTACCCCACCTCACAAGCCATCGGCGCACCACTGTTCCGcc                             <  2:932194/134‑1 (MQ=255)                                                                                                          ggAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCAAAAAAGAGAACTACCCCACCTCACAAGCCATCGGCGCACCACTGTTCCGcc                             >  1:932194/1‑134 (MQ=255)                                                                                                          ggAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCAAAAAAGAGAACTACCCCACCTCACAAGCCATCGGCGCACCACTGTTCCGCCAGATag                       >  1:824632/1‑140 (MQ=255)                                                                                                          ggAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCAAAAAAGAGAACTACCCCACCTCACAAGCCATCGGCGCACCACTGTTCCGCCAGATag                       >  2:839332/1‑140 (MQ=255)                                                                                                          ggAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCAAAAAAGAGAACTACCCCACCTCACAAGCCATCGGCGCACCACTGTTCCGCCAGATag                       >  1:983631/1‑140 (MQ=255)                                                                                                           gAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCAAAAAAGAGAACTACCCCACCTCACAAGCCATCGGCGCACCACTg                                    >  1:871455/1‑126 (MQ=255)                                                                                                           gAGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCAAAAAAGAGAACTACCCCACCTCACAAGCCATCGGCGCACCACTg                                    <  2:871455/126‑1 (MQ=255)                                                                                                            aGCTGTTGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCAAAAAAGAGAACTACCCCACCTCACAAGCCATCGGCGCACCACTGTTCCGCCAGATagaa                     >  1:791148/1‑140 (MQ=255)                                                                                                                 ttGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCAAAAAAGAGAACTACCCCACCTCACAAGCCATCGGCGCACCACTGTTCCGcc                             <  1:872082/127‑1 (MQ=255)                                                                                                                 ttGCGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCAAAAAAGAGAACTACCCCACCTCACAAGCCATCGGCGCACCACTGTTCCGcc                             >  2:872082/1‑127 (MQ=255)                                                                                                                    cGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCAAAAAAGAGAACTACCCCACCTCACAAGCCATCGGCGCACCACTGTTCCGCCAGATAGAAGAAAgcgg             <  1:5962/140‑1 (MQ=255)                                                                                                                    cGTAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCAAAAAAGAGAACTACCCCACCTCACAAGCCATCGGCGCACAACTGTTCCGCCAGATAGAAGAAAgcgg             <  1:45262/140‑1 (MQ=255)                                                                                                                      tAACATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCAAAAAAGAGAACTACCCCACCTCACAAGCCATCGGCGCACCACTGTTCCGCCAGATAGAAGAAAgcggcg           <  2:401529/140‑1 (MQ=255)                                                                                                                       aaCATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCAAAAAAGAGAACTACCCCACCTCACAAGCCATCGGCGCACCACTGTTCCGCCAGATAGAAGAAAGCGgcgc          <  1:211341/140‑1 (MQ=255)                                                                                                                       aaCATCCCGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCAAAAAAGAGAACTACCCCACCTCACAAGCCATCGGCGCACCACTGTTCCGCCAGATAGAAGAAAGCGgcgc          <  1:198978/140‑1 (MQ=255)                                                                                                                            cccGGGACTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCAAAAAAGAGAACTACCCCACCTCACAAGCCATCGGCGCACCACTGTTCCGCCAGATAGAAGAAAGCGGCGCAGATc     >  2:1068194/1‑140 (MQ=255)                                                                                                                            cccGGGACTTGAGTCAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCAAAAAAGAGAACTACCCCACCTCACAAGCCATCGGCGCACCACTGTTCCGCCAGATAGAAGAAAGCGGCGCAGATc     >  1:328276/1‑140 (MQ=255)                                                                                                                               gggaCTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCAAAAAAGAGAACTACCCCACCTCACAAGCCATCGGCGCACCACTGTTCCGCCAGATAGAAGAAAGCGGCGCAGATCtgg  <  2:983631/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                         CAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGGCTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGTACTTACGGTTTCAAAAAAGAGAACTACCCCACCTCACAAGCCATCGGCGCACCACTGTTCCGCCAGATAGAAGAAAGCGGCGCAGATCTGG  >  NC_000913/2356348‑2356613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑