Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,394,435 T→C A204A (GCA→GCG)  nuoL ← NADH:quinone oxidoreductase subunit L

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,394,4350TC94.0% 151.5 / 0.2 50A204A (GCA→GCG) nuoLNADH:quinone oxidoreductase subunit L
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base C (20/27);  total (21/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

GAGACAGGCGTCGGGCCCGCCATCGCGTCGGCAAGCCATGTCTGCAACGGCAACTGCGCAGATTTACCGACCGCACCGCCCAGCAGCATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGTGCCAGTTCCACCATTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGT  >  NC_000913/2394298‑2394572
                                                                                                                                         |                                                                                                                                         
gagaCAGGCGTCGGGCCCGCCATCGCGTCGGCAAGCCATGTCTGCAACGGCAACTGCGCAGATTTACCGACCGCACCGCCCAGCAGCATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGTGc                                                                                                                                         >  2:115439/1‑140 (MQ=255)
 agaCAGGCGTCGGGCCCGCCATCGCGTCGGCAAGCCATGTCTGCAACGGCAACTGCGCAGATTTACCGACCGCACCGCCCAGCAGCATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGCGcc                                                                                                                                        <  2:579579/140‑1 (MQ=255)
      ggCGTCGGGCCCGCCATCGCGTCGGCAAGCCATGTCTGCAACGGCAACTGCGCAGATTTACCGACCGCACCGCCCAGCAGCATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGCGCCAGTTc                                                                                                                                   >  1:805391/1‑140 (MQ=255)
           cGGGCCCGCCATCGCGTCGGCAAGCCATGTCTGCAACGGCAACTGCGCAGATTTACCGACCGCACCGCCCAGCAGCATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGCGCCAGTTccacca                                                                                                                              >  2:559547/1‑140 (MQ=255)
                  gCCATCGCGTCGGCAAGCCATGTCTGCAACGGCAACTGCGCAGATTTACCGACCGCACCGCCCAGCAGCATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGCGCCAGTTCCACCATTTcgcg                                                                                                                       <  2:77642/140‑1 (MQ=255)
                          gTCGGCAAGCCATGTCTGCAACGGCAACTGCGCAGATTTACCGACCGCACCGCCCAGCAGTATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGCGCCAGTTCCACCATTTCGCGGAAGTTCa                                                                                                               <  2:1027936/140‑1 (MQ=255)
                          gTCGGCAAGCCATGTCTGCAACGGCAACTGCGCAGATTTACCGACCGCACCGCCCAGCAGCATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGCGCCAGTTCCACCATTTCGCGGAAGTTCa                                                                                                               <  1:1102166/140‑1 (MQ=255)
                           tCGGCAAGCCATGTCTGCAACGGCAACTGCGCAGATTTACCGACCGCACCGCCCAGCAGCATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGCGCCAGTTCCACCATTTCGCGGAAGTTCAg                                                                                                              <  2:437034/140‑1 (MQ=255)
                                   ccATGTCTGCAACGGCAACTGCGCAGATTTACCGACCGCACCGCCCAGCAGCATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGCGCCAGTTCCACCATTTCGCGGAAGTTCAGGGTGCCCa                                                                                                      <  1:817248/140‑1 (MQ=255)
                                      tGTCTGCAACGGCAACTGCGCAGATTTACCGACCGCACCGCCCAGCAGCATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGCGCCAGTTCCACCATTTCGCGGAAGTTCAGGGTGCCCAGtt                                                                                                   <  2:578205/140‑1 (MQ=255)
                                       gTCTGCAACGGCAACTGCGCAGATTTACCGACCGCACCGCCCAGCAGCATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGTGCTGGCGCCAGTTCCACCATTTCGCGGAAGTTCAGGGTGCCCAGTTc                                                                                                  <  1:562655/140‑1 (MQ=255)
                                       gTCTGCAACGGCAACTGCGCAGATTTACCGACCGCACCGCCCAGCAGCATCAGCGTCGCCCACATCAGCATGTTATTCCCGTCAGCAAAGTGCGCTCGCGCCAGTTCCACCATTTCGCGGAAGTTCAGGGTGCCCAGTTc                                                                                                  <  2:1024755/140‑1 (MQ=255)
                                        tCTGCAACGGCAACTGCGCAGATTTACCGACCGCACCGCCCAGCAGCATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGCGCCAGTTCCACCATTTCGCGGAAGTTCa                                                                                                               <  1:355315/126‑1 (MQ=255)
                                        tCTGCAACGGCAACTGCGCAGATTTACCGACCGCACCGCCCAGCAGCATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGCGCCAGTTCCACCATTTCGCGGAAGTTCa                                                                                                               >  2:355315/1‑126 (MQ=255)
                                            cAACGGCAACTGCGCAGATTTACCGACCGCACCGCCCAGCAGCATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGCGCCAGTTCCACCATTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGt                                                                                             <  2:985581/140‑1 (MQ=255)
                                                ggCAACTGCGCAGATTTACCGACCGCACCGCCCAGCAGCATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGCGCCAGTTCCACCATTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAg                                                                                         >  2:615167/1‑140 (MQ=255)
                                                 gCAACTGCGCAGATTTACCGACCGCACCGCCCAGCAGCATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGCGCCAGTTCCACCATTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGa                                                                                        >  2:212323/1‑140 (MQ=255)
                                                 gCAACTGCGCAGATTTACCGACCGCACCGCCCAGCAGCATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGCGCCAGTTCCACCATTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGa                                                                                        >  1:117715/1‑140 (MQ=255)
                                                      tGCGCAGATTTACCGACCGCACCGCCCAGCAGCATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGTGCCAGTTCCACCATTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGaa                                                                                   <  1:1031479/140‑1 (MQ=255)
                                                      tGCGCAGATTTACCGACCGCACCGCCCAGCAGCATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGCGCCAGTTCCACCATTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGaa                                                                                   <  2:103312/140‑1 (MQ=255)
                                                              tttACCGACCGCACCGCCCAGCAGCATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGTTGCCAGTTCCACCATTTCGCGGAAGTTCAGGGTGACCCTTTCGTTATAAAGAATGAGAAGTGCGa                                                                           <  2:835880/140‑1 (MQ=255)
                                                                  ccgaccgCACCGCCCAGCAGCATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGCGCCAGTTCCACCATTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGc                                                                       >  2:360735/1‑140 (MQ=255)
                                                                   cgaccgCACCGCCCAGCAGCATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGCGCCAGTTCCACCATTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCg                                                                      <  1:360735/140‑1 (MQ=255)
                                                                      ccgcaccgcCCAGCAGCATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGCGCCAGTTCCACCATTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAgg                                                                   <  2:805391/140‑1 (MQ=255)
                                                                         caccgcCCAGCAGCATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGCGCCAGTTCCACCATTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAac                                                                >  2:665297/1‑140 (MQ=255)
                                                                         caccgcCCAGAAACGTCAGCGTCCCCCACCTCAGCATGTTATTGACGTCAGGAAGACGAGCTGGTGCCAGTTCCACCCTTTCGTGGAAGTTCAGGGTGCCCAGATCGTTGTAAAGACTGAGGAGTGCGGAGGCGAGGagc                                                                >  2:204826/1‑138 (MQ=255)
                                                                          accgcCCAGCAGCATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGCGCCAGTTCCACCATTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAaca                                                               <  1:212323/140‑1 (MQ=255)
                                                                                cagcagCATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGCGCCAGTTCCACCATTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCAc                                                         <  1:665297/140‑1 (MQ=255)
                                                                                cagcagCATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGCGCCAGTTCCACCATTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCAc                                                         <  2:117715/140‑1 (MQ=255)
                                                                                        tCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGCGCCAGTTCCACCATTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACgg                                                 >  2:417224/1‑140 (MQ=255)
                                                                                                gCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGCGCCAGTTCCACCATTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCacgac                                         >  1:883880/1‑140 (MQ=255)
                                                                                                     caTCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGCGCCAGTTCCACCATTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACg                                    <  1:417224/140‑1 (MQ=255)
                                                                                                      aTCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGCGCCAGTTCCACCATTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGc                                   >  1:1087513/1‑140 (MQ=255)
                                                                                                                tATTGCCGTCAGCAAAGTGCGCTGGCGCCAGTTCCACCATTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGcc                         >  1:556176/1‑140 (MQ=255)
                                                                                                                  ttGCCGTCAGCAAAGTGCGCTGGCGCCAGTTCCACCATTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGc                       >  1:282703/1‑140 (MQ=255)
                                                                                                                     ccGTCAGCAAAGTGCGCTGGCGCCAGTTCCACCATTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTgc                    >  2:341240/1‑140 (MQ=255)
                                                                                                                       gTCAGCAAAGTGCGCTGGCGCCAGTTCCACCATTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTgcgc                  >  2:908740/1‑140 (MQ=255)
                                                                                                                       gTCAGCAAAGTGCGCTGGCGCCAGTTCCACCATTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTgcgc                  <  1:261480/140‑1 (MQ=255)
                                                                                                                        tCAGCAAAGTGCGCTGGCGCCAGTTCCACCATTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGcc                 >  1:498348/1‑140 (MQ=255)
                                                                                                                        tCAGCAAAGTGCGCTGGCGCCAGTTCCACCATTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGcc                 >  2:1053199/1‑140 (MQ=255)
                                                                                                                          aGCAAAGTGCGCTGGCGCCAGTTCCACCATTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCAt               >  2:1007291/1‑140 (MQ=255)
                                                                                                                              aaGTGCGCTGGCGCCAGTTCCACCATTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCAttctt           <  2:737743/140‑1 (MQ=255)
                                                                                                                                 tGCGCTGGCGCCAGTTCCACCATTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCgg        <  1:1007291/140‑1 (MQ=255)
                                                                                                                                 tGCGCTGGCGCCAGTTCCACCATTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCgg        >  1:951867/1‑140 (MQ=255)
                                                                                                                                   cgcTGGCGCCAGTTCCACCATTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGAt      >  2:578113/1‑140 (MQ=255)
                                                                                                                                   cgcTGGCGCCAGTTCCACCATTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGAt      <  2:498348/140‑1 (MQ=255)
                                                                                                                                   cgcTGGCGCCAGTTCCACCATTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGAt      <  2:370503/140‑1 (MQ=255)
                                                                                                                                   cgcTGGCGCCAGTTCCACCATTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGAt      <  2:35941/140‑1 (MQ=255)
                                                                                                                                   cgcTGGCGCCAGTTCCACCATTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGAt      <  2:883880/140‑1 (MQ=255)
                                                                                                                                   cgcTGGCGCCAGTTCCACCATTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGAt      <  2:1054357/140‑1 (MQ=255)
                                                                                                                                   cgcTGGCGCCAGTTCCACCATTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGAt      <  1:559547/140‑1 (MQ=255)
                                                                                                                                       ggTGCCAGTTCCACCATTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGgt  <  1:115439/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                         
GAGACAGGCGTCGGGCCCGCCATCGCGTCGGCAAGCCATGTCTGCAACGGCAACTGCGCAGATTTACCGACCGCACCGCCCAGCAGCATCAGCGTCGCCCACATCAGCATGTTATTGCCGTCAGCAAAGTGCGCTGGTGCCAGTTCCACCATTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGT  >  NC_000913/2394298‑2394572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑