Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,408,087	A→C	N176T (AAC→ACC)	alaA →	glutamate‑‑pyruvate aminotransferase AlaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,408,087	0	A	C	100.0%	137.6 / NA	40	N176T (AAC→ACC)	alaA	glutamate‑‑pyruvate aminotransferase AlaA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (19/21);  total (19/21)

CTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGTGGGATCGTTATTATCAACCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAATGGAGATTGTGGAGATTGCACGTCAGCATAATCTCATTATCTTCGCCGATGAAATTTATGACAAAATTCTCTACGACGACGCTGAGCATCA  >  NC_000913/2407951‑2408222                                                                                                                                         |                                                                                                                                        ctctGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGTGGGATCGTTATTATCAcccc                                                                                                                                      >  2:546903/1‑140 (MQ=255)  tctGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGTGGGATCGTTATTATCACCCCa                                                                                                                                     >  1:9281/1‑140 (MQ=255)   ctGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGTGGGATCGTTATTATCACCCCaa                                                                                                                                    <  2:41654/140‑1 (MQ=255)        ccGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGTGGGATCGTTATTATCACCCCAAATAAc                                                                                                                               >  2:535100/1‑140 (MQ=255)            ggcggTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGTGGGATCGTTATTATCACCCCAAATAACCCaa                                                                                                                           >  1:255600/1‑140 (MQ=255)             gcggTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGTGGGATCGTTATTATCACCCCAAATAACCCAAc                                                                                                                          <  1:535100/140‑1 (MQ=255)               ggTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGTGGGATCGTTATTATCACCCCAAATAACCCAACCg                                                                                                                        <  2:76342/140‑1 (MQ=255)                gTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGTGGGATCGTTATTATCACCCCAAATAACCCAACCgg                                                                                                                       >  1:733705/1‑140 (MQ=255)                  ttCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTATGCGTGGGATCGTTATTATCACCCCAAATAACCCAACCGgcg                                                                                                                     <  1:871476/140‑1 (MQ=255)                  ttCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGTGGGATCGTTATTATCACCCCAAATAACCCAACCGgcg                                                                                                                     <  1:165188/140‑1 (MQ=255)                  ttCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGTGGGATCGTTATTATCACCCCAAATAACCCAACCGgcg                                                                                                                     <  2:766509/140‑1 (MQ=255)                  ttCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGTGGGATCGTTATTATCACCCCAAATAACCCAACCGgcg                                                                                                                     <  2:733705/140‑1 (MQ=255)                  ttCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGTGGGATCGTTATTATCACCCCAAATAACCCAACCGgcg                                                                                                                     <  2:317342/140‑1 (MQ=255)                    cGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGTGGGATCGTTATTATCACCCCAAATAACCCAACCGGcgcg                                                                                                                   <  2:987089/140‑1 (MQ=255)                    cGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGTGGGATCGTTATTATCACCCCAAATAACCCAACCGGcgcg                                                                                                                   <  1:466297/140‑1 (MQ=255)                      cTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGTCTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGTGGGATCGTTATTATCGCCCCAAATAACCCAACCGGCGCGGt                                                                                                                 <  2:532336/140‑1 (MQ=255)                         tCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGTGGGATCGTTATTATCACCCCAAATAACCCAACCGGCGCGGtata                                                                                                              <  2:9281/140‑1 (MQ=255)                                gTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGTGGGATCGTTATTATCACCCCAAATAACCCAACCGGCGCGGTATATTCCaaa                                                                                                       <  1:647448/140‑1 (MQ=255)                                   aaGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGTGGGATCGTTATTATCACCCCAAATAACCCAACCGgcg                                                                                                                     <  1:1127183/123‑1 (MQ=255)                                   aaGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGTGGGATCGTTATTATCACCCCAAATAACCCAACCGgcg                                                                                                                     >  2:1127183/1‑123 (MQ=255)                                           cATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGTGGGATCGTTATTATCACCCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGc                                                                                                   >  2:576704/1‑133 (MQ=255)                                           cATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGTGGGATCGTTATTATCACCCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGc                                                                                                   <  1:576704/133‑1 (MQ=255)                                           cATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGTGGGATCGTTATTATCACCCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAAt                                                                                            <  2:255600/140‑1 (MQ=255)                                                tCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGTGGGATCGTTATTATCACCCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAAtggaga                                                                                       >  2:565560/1‑140 (MQ=255)                                                   ttGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGTGGGATCGTTATTATCACCCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAAtggagattg                                                                                    >  1:590502/1‑140 (MQ=255)                                                   ttGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGTGGGATCGTTATTATCACCCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAAtggagattg                                                                                    >  1:769911/1‑140 (MQ=255)                                                   ttGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGTGGGATCGTTATTATCACCCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAAtgg                                                                                          <  1:809674/134‑1 (MQ=255)                                                   ttGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGTGGGATCGTTATTATCACCCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAAtgg                                                                                          >  2:809674/1‑134 (MQ=255)                                                                       ggTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGTGGGATCGTTATTATCACCCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAATGGAGATTGTGGAGATTGCACGTCAGCAt                                                                <  1:546903/140‑1 (MQ=255)                                                                        gTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGTGGGATCGTTATTATCACCCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAATGGAGATTGTGGAGATTGCACGTCAGCATa                                                               >  1:876311/1‑140 (MQ=255)                                                                              ggACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGTGGGATCGTTATTATCACCCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAATGGAGATTGTGGAGATTGCACGTCAGCATAAtctc                                                          >  1:961362/1‑139 (MQ=255)                                                                              ggACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGTGGGATCGTTATTATCACCCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAATGGAGATTGTGGAGATTGCACGTCAGCATAAtctc                                                          <  2:961362/139‑1 (MQ=255)                                                                              ggACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGTGGGATCGTTATTATCACCCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAATGGAGATTGTGGAGATTGCACGTCAGCATAATCTCa                                                         <  1:78119/140‑1 (MQ=255)                                                                                   tCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGTGGGATCGTTATTATCACCCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAATGGAGATTGTGGAGATTGCACGTCAGCATAATCTCATTATc                                                    >  2:514721/1‑140 (MQ=255)                                                                                   tCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGTGGGATCGTTATTATCACCCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAATGGAGATTGTGGAGATTGCACGTCAGCATAATCTCATTATc                                                    >  2:111751/1‑140 (MQ=255)                                                                                                 gcTAAAATTACGCCTCGTACGCGTGGGATCGTTATTATCACCCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAATGGAGATTGTGGAGATTGCACGTCAGCATAATCTCATTATCTTCGCCGATGAAAt                                      >  1:310902/1‑140 (MQ=255)                                                                                                                      cgTGGGATCGTTATTATCACCCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAATGGAGATTGTGGAGATTGCACGTCAGCATAATCTCATTATCTTCGCCGATGAAATTTATGACAAAATTCTCTacga                 <  2:310902/140‑1 (MQ=255)                                                                                                                            aTCGTTATTATCACCCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAATGGAGATTGTGGAGATTGCACGTCAGCATAATCTCATTATCTTCGCCGATGAAATTTATGACAAAATTCTCTACGACGACGc           >  1:604821/1‑140 (MQ=255)                                                                                                                                     atCACCCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAATGGAGATTGTGGAGATTGCACGTCAGCATAATCTCATTATCTTCGCCGATGAAATTTATGACAAAATTCTCTACGACGACGCTGAGCATCa  >  2:683741/1‑140 (MQ=255)                                                                                                                                     atCACCCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAATGGAGATTGTGGAGATTGCACGTCAGCATAATCTCATTATCTTCGCCGATGAAATTTATGACAAAATTCTCTACGACGACGCTGAGCATCa  >  2:1135285/1‑140 (MQ=255)                                                                                                                                     atCACCCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAATGGAGATTGTGGAGATTGCACGTCAGCATAATCTCATTATCTTCGCCGATGAAATTTATGACAAAATTCTCTACGACGACGCTGAGCATCa  >  2:868279/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        CTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGTGGGATCGTTATTATCAACCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAATGGAGATTGTGGAGATTGCACGTCAGCATAATCTCATTATCTTCGCCGATGAAATTTATGACAAAATTCTCTACGACGACGCTGAGCATCA  >  NC_000913/2407951‑2408222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑