Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,413,200	T→C	intergenic (‑68/‑270)	yfbV ← / → ackA	UPF0208 membrane protein YfbV/acetate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,413,200	0	T	C	100.0%	175.3 / NA	50	intergenic (‑68/‑270)	yfbV/ackA	UPF0208 membrane protein YfbV/acetate kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/23);  total (27/23)

TCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCAACTTCACATATAAAGATTCAAAAATTTGTGCAAATTCACAACTCAGCGGGACAACGTTCAAAACATTTTGTCTTCCATACCCACTATCAGGTATCCTTTAGCAGCCTGAAGGCCTAAGTAGTACATATTCATTGAGTCGTCAAATTCATATACATTATGCCATTGGCTGAAAATTACGCAAAATGGCATAG  >  NC_000913/2413079‑2413334                                                                                                                          |                                                                                                                                       tCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCAACTTCACATATAAAGATTCAAAAATTTGTGCAAATTCACAACTCAGCGGGACAACGCTCAAAACATTTTGt                                                                                                                          >  2:355973/1‑136 (MQ=255) tCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCAACTTCACATATAAAGATTCAAAAATTTGTGCAAATTCACAACTCAGCGGGACAACGCTCAAAACATTTTGt                                                                                                                          <  1:355973/136‑1 (MQ=255) tCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCAACTTCACATATAAAGATTCAAAAATTTGTGCAAATTCACAACTCAGCGGGACAACGCTCAAAACATTTTGTCTTc                                                                                                                      >  2:337062/1‑140 (MQ=255)      ggCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCAACTTCACATATAAAGATTCAAAAATTTGTGCAAATTCACAACTCAGCGGGACAACGCTCAAAACATTTTGTCTTCCATAc                                                                                                                 >  1:838007/1‑140 (MQ=255)      ggCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCAACTTCACATATAAAGATTCAAAAATTTGTGCAAATTCACAACTCAGCGGGACAACGCTCAAAACATTTTGTCTTCCATAc                                                                                                                 >  1:404259/1‑140 (MQ=255)       gCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCAACTTCACATATAAAGATTCAAAAATTTGTGCAAATTCACAACTCAGCGGGACAACGCTCAAAACATTTTGTCTTCCATAcc                                                                                                                <  1:116686/140‑1 (MQ=255)           aaCAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCAACTTCACATATAAAGATTCAAAAATTTGTGCAAATTCACAACTCAGCGGGACAACGCTCAAAACATTTTGTCTTCCATACCCACt                                                                                                            >  2:1143697/1‑140 (MQ=255)                 cTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCAACTTCACATATAAAGATTCAAAAATTTGTGCAAATTCACAACTCAGCGGGACAACGCTCAAAACATTTTGTCTTCCATACCCACTATCAgg                                                                                                      <  1:337062/140‑1 (MQ=255)                     aaaaaTTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCAACTTCACATATAAAGATTCAAAAATTTGTGCAAATTCACAACTCAGCGGGACAACGCTCAAAACATTTTGTCTTCCATACCCACTATCAgg                                                                                                      >  2:462489/1‑136 (MQ=255)                     aaaaaTTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCAACTTCACATATAAAGATTCAAAAATTTGTGCAAATTCACAACTCAGCGGGACAACGCTCAAAACATTTTGTCTTCCATACCCACTATCAgg                                                                                                      <  1:462489/136‑1 (MQ=255)                          ttAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCAACTTCACATATAAAGATTCAAAAATTTGTGCAAATTCACAACTCAGCGGGACAACGCTCAAAACATTTTGTCTTCCATACCCACTATCAGGTATCCTTTa                                                                                             >  2:353692/1‑140 (MQ=255)                           tAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCAACTTCACATATAAAGATTCAAAAATTTGTGCAAATTCACAACTCAGCGGGACAACGCTCAAAACATTTTGTCTTCCATACCCACTATCAGGTATCCTTTag                                                                                            <  1:1143697/140‑1 (MQ=255)                                       tATCCGGCGTTGACATGCTTCACCTCAACTTCACATATAAAGATTCAAAAATTTGTGCAAATTCACAACTCAGCGGGACAACGCTCAAAACATTTTGTCTTCCATACCCACTATCAGGTATCCTTTAGCAGCCTGAAGGc                                                                                <  2:678216/140‑1 (MQ=255)                                         tCCGGCGTTGACATGCTTCACCTCAACTTCACATATAAAGATTCAAAAATTTGTGCAAATTCACAACTCAGCGGGACAACGCTCAAAACATTTTGTCTTCCATACCCACTATCAGGTATCCTTTAGCAGCCTGAAGGCCt                                                                              <  1:708534/140‑1 (MQ=255)                                           cGGCGTTGACATGCTTCACCTCAACTTCACATATAAAGATTCAAAAATTTGTGCAAATTCACAACTCAGCGGGACAACGCTCAAAACATTTTGTCTTCCATACCCACTATCAGGTATCCTTTAGCAGCCTGAAGGccaag                                                                            <  2:400309/140‑4 (MQ=255)                                           cGGCGTTGACATGCTTCACCTCAACTTCACATATAAAGATTCAAAAATTTGTGCAAATTCACAACTCAGCGGGACAACGCTCAAAACATTTTGTCTTCCATACCCACTATCAGGTATCCTTTAGCAGCCTGAAGGCCTaa                                                                            >  1:10785/1‑140 (MQ=255)                                                ttGACATGCTTCACCTCAACTTCACACATAAAGATTCAAAAATTTGTGCAAATTCACAACTCAGCGGGACAACGCTCAAAACATTTTGTCTTCCATACCCACTATCAGGTATCCTTTAGCAGCCTGAAGGCCTAagtagt                                                                       >  2:428471/1‑140 (MQ=255)                                                   aCATGCTTCACCTCAACTTCACATATAAAGATTCAAAAATTTGTGCAAATTCACAACTCAGCGGGACAACGCTCAAAACATTTTGTCTTCCATACCCACTATCAGGTATCCTTTAGCAGCCTGAAGGCCTAAGTAGTACa                                                                    >  1:596953/1‑140 (MQ=255)                                                   aCATGCTTCACCTCAACTTCACATATAAAGATTCAAAAATTTGTGCAAATTCACAACTCAGCGGGACAACGCTCAAAACATTTTGTCTTCCATACCCACTATCAGGTATCCTTTAGCAGCCTGAAGGCCTAAGTAGTACa                                                                    >  1:70634/1‑140 (MQ=255)                                                        cTTCACCTCAACTTCACATATAAAGATTCAAAAATTTGTGCAAATTCACAACTCAGCGGGACAACGCTCAAAACATTTTGTCTTCCATACCCACTATCAGGTATCCTTTAGCAGCCTGAAGGCCTAAGTAGTACATATTc                                                               >  2:340532/1‑140 (MQ=255)                                                             ccTCAACTTCACATATAAAGATTCAAAAATTTGTGCAAATTCACAACTCAGCGGGACAACGCTCAAAACATTTTGTCTTCCATACCCACTATCAGGTATCCTTTAGCAGCCTGAAGGCCTAAGTAGTACATATTCATTGa                                                          >  1:800988/1‑140 (MQ=255)                                                                cAACTTCACATATAAAGATTCAAAAATTTGTGCAAATTCACAACTCAGCGGGACAACGCTCAAAACATTTTGTCTTCCATACCCACTATCAGGTATCCTTTAGCAGCCTGAAGGCCTAagtag                                                                        >  1:41115/1‑123 (MQ=255)                                                                cAACTTCACATATAAAGATTCAAAAATTTGTGCAAATTCACAACTCAGCGGGACAACGCTCAAAACATTTTGTCTTCCATACCCACTATCAGGTATCCTTTAGCAGCCTGAAGGCCTAagtag                                                                        <  2:41115/123‑1 (MQ=255)                                                                 aaCTTCACATATAAAGATTCAAAAATTTGTGCAAATTCACAACTCAGCGGGACAACGCTCAAAACATTTTGTCTTCCATACCCACTATCAGGTATCCTTTAGCAGCCTGAAGGCCTAAGTAGTACATATTCATTGAgtcg                                                      >  1:681236/1‑140 (MQ=255)                                                                  aCTTCACATATAAAGATTCAAAAATTTGTGCAAATTCACAACTCAGCGGGACAACGCTCAAAACATTTTGTCTTCCATACCCACTATCAGGTATCCTTTAGCAGCCTGAAGGCCTAAGTAGTACATATTCATTGAgtcgt                                                     <  2:838007/140‑1 (MQ=255)                                                                      cacaTATAAAGATTCAAAAATTTGTGCAAATTCACAACTCAGCGGGACAACGCTCAAAACATTTTGTCTTCCATACCCACTATCAGGTATCCTTTAGCAGCCTGAAGGCCTAAGTAGTACATATTCATTGAGTCGTCaaa                                                 <  2:70634/140‑1 (MQ=255)                                                                      cacaTATAAAGATTCAAAAATTTGTGCAAATTCACAACTCAGCGGGACAACGCTCAAAACATTTTGTCTTCCATACCCACTATCAGGTATCCTTTAGCAGCCTGAAGGCCTAAGTAGTACATATTCATTGAGTCGTCaaa                                                 <  1:247029/140‑1 (MQ=255)                                                                      cacaTATAAAGATTCAAAAATTTGTGCAAATTCACAACTCAGCGGGACAACGCTCAAAACATTTTGTCTTCCATACCCACTATCAGGTATCCTATAGCAGCCTGAAGGCCTAAGTAGTACATATTCATTGAGTCGTCaaa                                                 <  2:404259/140‑1 (MQ=255)                                                                           ataAAGATTCAAAAATTTGTGCAAATTCACAACTCAGCGGGACAACGCTCAAAACATTTTGTCTTCCATACCCACTATCAGGTATCCTTTAGCAGCCTGAAGGCCTAAGTAGTACATATTCATTGAGTCGTCAAATTCat                                            <  2:10785/140‑1 (MQ=255)                                                                            taAAGATTCAAAAATTTGTGCAAATTCACAACTCAGCGGGACAACGCTCAAAACATTTTGTCTTCCATACCCACTATCAGGTATCCTTTAGCAGCCTGAAGGCCTAAGTAGTACATATTCATTGAGTCGTCAAATTCata                                           <  1:340532/140‑1 (MQ=255)                                                                            taAAGATTCAAAAATTTGTGCAAATTCACAACTCAGCGGGACAACGCTCAAAACATTTTGTCTTCCATACCCACTATCAGGTATCCTTTAGCAGCCTGAAGGCCTAAGTAGTACATATTCATTGAGTCGTCAAATTCata                                           <  1:428471/140‑1 (MQ=255)                                                                              aaGATTCAAAAATTTGTGCAAATTCACAACTCAGCGGGACAACGCTCAAAACATTTTGTCTTCCATACCCACTATCAGGTATCCTTTAGCAGCCTGAAGGCCTAAGTAGTACATATTCATTGAGTCGTCAAATTCAtata                                         >  2:758501/1‑140 (MQ=255)                                                                                 aTTCAAAAATTTGTGCAAATTCACAACTCAGCGGGACAACGCTCAAAACATTTTGTCTTCCATACCCACTATCAGGTATCCTTTAGCAGCCTGAAGGCCTAAGTAGTACATATTCATTGAGTCGTCAAATTCata                                           <  1:264135/135‑1 (MQ=255)                                                                                 aTTCAAAAATTTGTGCAAATTCACAACTCAGCGGGACAACGCTCAAAACATTTTGTCTTCCATACCCACTATCAGGTATCCTTTAGCAGCCTGAAGGCCTAAGTAGTACATATTCATTGAGTCGTCAAATTCata                                           >  2:264135/1‑135 (MQ=255)                                                                                   tCAAAAATTTGTGCAAATTCACAACTCAGCGGGACAACGCTCAAAACATTTTGTCTTCCATACCCACTATCAGGTATCCTTTAGCAGCCTGAAGGCCTAAGTAGTACATATTCATTGAgtcgtc                                                    <  2:995288/124‑1 (MQ=255)                                                                                   tCAAAAATTTGTGCAAATTCACAACTCAGCGGGACAACGCTCAAAACATTTTGTCTTCCATACCCACTATCAGGTATCCTTTAGCAGCCTGAAGGCCTAAGTAGTACATATTCATTGAgtcgtc                                                    >  1:995288/1‑124 (MQ=255)                                                                                   tCAAAAATTTGTGCAAATTCACAACTCAGCGGGACAACGCTCAAAACATTTTGTCTTCCATACCCACTATCAGGTATCCTTTAGCAGCCTGAAGGCCTAAGTAGTACATATTCATTGAGTCGTCAAATTCATATACATTa                                    >  2:73054/1‑140 (MQ=255)                                                                                     aaaaaTTTGTGCAAATTCACAACTCAGCGGGACAACGCTCAAAACATTTTGTCTTCCATACCCACTATCAGGTATCCTTTAGCAGCCTGAAGGCCTAAGTAGTACATATTCATTGAGTCGTCAAATTCata                                           <  2:1145344/131‑1 (MQ=255)                                                                                     aaaaaTTTGTGCAAATTCACAACTCAGCGGGACAACGCTCAAAACATTTTGTCTTCCATACCCACTATCAGGTATCCTTTAGCAGCCTGAAGGCCTAAGTAGTACATATTCATTGAGTCGTCAAATTCata                                           >  1:1145344/1‑131 (MQ=255)                                                                                         aTTTGTGCAAATTCACAACTCAGCGGGACAACGCTCAAAACATTTTGTCTTCCATACCCACTATCAGGTATCCTTTAGCAGCCTGAAGGCCTAAGTAGTACATATTCATTGAGTCGTCAAATTCATATACATTATGCCAt                              <  1:36433/140‑1 (MQ=255)                                                                                                        caACTCAGCGGGACAACGCTCAAAACATTTTGTCTTCCATACCCACTATCAGGTATCCTTTAGCAGCCTGAAGGCCTAAGTAGTACATATTCATTGAGTCGTCAAATTCATATACATTATGCCATTGGCTGAAAATTACg               <  1:758501/140‑1 (MQ=255)                                                                                                           cTCAGCGGGACAACGCTCAAAACATTTTGTCTTCCATACCCACTATCAGGTATCCTTTAGCAGCCTGAAGGCCTAAGTAGTACATATTCATTGAGTCGTCAAATTCATATACATTATGCCATTGGCTGAAAATTACGCaa            >  1:990607/1‑140 (MQ=255)                                                                                                              aGCGGGACAACGCTCAAAACATTTTGTCTTCCATACCCACTATCAGGTATCCTTTAGCAGCCTGAAGGCCTAAGTAGTACATATTCATTGAGTCGTCAAATTCATATACATTATGCCATTGGCTGAAAATTACGCaaa           <  2:384089/138‑1 (MQ=255)                                                                                                              aGCGGGACAACGCTCAAAACATTTTGTCTTCCATACCCACTATCAGGTATCCTTTAGCAGCCTGAAGGCCTAAGTAGTACATATTCATTGAGTCGTCAAATTCATATACATTATGCCATTGGCTGAAAATTACGCaaa           >  1:384089/1‑138 (MQ=255)                                                                                                              aGCGGGACAACGCTCAAAACATTTTGTCTTCCATACCCACTATCAGGTATCCTTTAGCAGCCTGAAGGCCTAAGTAGTACATATTCATTGAGTCGTCAAATTCATATACATTATGCCATTGGCTGAAAATTACGCAAAAt         >  1:253940/1‑140 (MQ=255)                                                                                                                cGGGACAACGCTCAAAACATTTTGTCTTCCATACCCACTATCAGGTATCCTTTAGCAGCCTGAAGGCCTAAGTAGTACATATTCATTGAGTCGTCAAATTCATATACATTATGCCATTGGCTGAAAATTACGCAAAATgg       >  2:79488/1‑140 (MQ=255)                                                                                                                  ggACAACGCTCAAAACATTTTGTCTTCCATACCCACTATCAGGTATCCTTTAGCAGCCTGAAGGCCTAAGTAGTACATATTCATTGAGTCGTCAAATTCATATACATTATGCCATTGGCTGAAAATTACGCAAAATGGCa     <  1:353692/140‑1 (MQ=255)                                                                                                                     cAACGCTCAAAACATTTTGTCTTCCATACCCACTATCAGGTATCCTTTAGCAGCCTGAAGGCCTAAGTAGTACATATTCATTGAGTCGTCAAATTCATATACATTATGCCATTGGCTGAAAATTACGCAAAATGGCATAg  >  2:345987/1‑140 (MQ=255)                                                                                                                     cAACGCTCAAAACATTTTGTCTTCCATACCCACTATCAGGTATCCTTTAGCAGCCTGAAGGCCTAAGTAGTACATATTCATTGAGTCGTCAAATTCATATACATTATGCCATTGGCTGAAAATTACGCAAAATGGCATAg  >  1:734686/1‑140 (MQ=255)                                                                                                                     cAACGCTCAAAACATTTTGTCTTCCATACCCACTATCAGGTATCCTTTAGCAGCCTGAAGGCCTAAGTAGTACATATTCATTGAGTCGTCAAATTCATATACATTATGCCATTGGCTGAAAATTACGCAAAATGGCATAg  >  2:807093/1‑140 (MQ=255)                                                                                                                          |                                                                                                                                       TCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATGCTTCACCTCAACTTCACATATAAAGATTCAAAAATTTGTGCAAATTCACAACTCAGCGGGACAACGTTCAAAACATTTTGTCTTCCATACCCACTATCAGGTATCCTTTAGCAGCCTGAAGGCCTAAGTAGTACATATTCATTGAGTCGTCAAATTCATATACATTATGCCATTGGCTGAAAATTACGCAAAATGGCATAG  >  NC_000913/2413079‑2413334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑