Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,446,579	T→C	T299A (ACC→GCC)	aroC ←	chorismate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,446,579	0	T	C	100.0%	227.4 / NA	66	T299A (ACC→GCC)	aroC	chorismate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/28);  total (38/28)

GTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑TAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCA  >  NC_000913/2446445‑2446713                                                                                                                                        |                                                                                                                                       gTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGc                                                                                                                                   >  1:303584/1‑140 (MQ=255)  tGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCt                                                                                                                                  >  2:615998/1‑140 (MQ=255)  tGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCt                                                                                                                                  >  2:769825/1‑140 (MQ=255)   gATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTg                                                                                                                                 >  2:1069151/1‑140 (MQ=255)       cATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAgg                                                                                                                             >  1:491184/1‑140 (MQ=255)        aTTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGt                                                                                                                            <  1:769825/140‑1 (MQ=255)              aCGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGttt                                                                                                                      >  2:793005/1‑140 (MQ=255)                gATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCa                                                                                                                    >  2:304997/1‑140 (MQ=255)                gATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCa                                                                                                                    <  1:304997/140‑1 (MQ=255)                 atcaccAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAg                                                                                                                   >  1:281718/5‑140 (MQ=255)                 aTCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAg                                                                                                                   <  2:491184/140‑1 (MQ=255)                  tCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAgc                                                                                                                  <  1:670797/140‑1 (MQ=255)                  tCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAgc                                                                                                                  <  2:766005/140‑1 (MQ=255)                  tCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAgc                                                                                                                  >  1:288445/1‑140 (MQ=255)                   caccAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAgcg                                                                                                                 <  2:281718/138‑1 (MQ=255)                       aGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCat                                                                                                             >  1:988708/1‑140 (MQ=255)                          aTCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATg                                                                                                          >  2:690543/1‑140 (MQ=255)                            cGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATggg                                                                                                        <  2:988708/140‑1 (MQ=255)                            cGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTCGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATggg                                                                                                        <  2:1002804/140‑1 (MQ=255)                              cTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCa                                                                                                      >  2:134423/1‑140 (MQ=255)                              cTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCa                                                                                                      >  2:832816/1‑140 (MQ=255)                                    cGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGAtt                                                                                                >  2:807741/1‑140 (MQ=255)                                    cGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGAtt                                                                                                >  1:211379/1‑140 (MQ=255)                                           cACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGcc                                                                                         >  2:714020/1‑140 (MQ=255)                                           cACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGcc                                                                                         <  1:793005/140‑1 (MQ=255)                                           cACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGcc                                                                                         <  1:714020/140‑1 (MQ=255)                                           cACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGcc                                                                                         <  1:69411/140‑1 (MQ=255)                                           cACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGcc                                                                                         <  1:690543/140‑1 (MQ=255)                                           cACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGcc                                                                                         >  2:536133/1‑140 (MQ=255)                                            aCTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGccc                                                                                        <  2:303584/140‑1 (MQ=255)                                            aCTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGccc                                                                                        <  1:807741/140‑1 (MQ=255)                                            aCTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGccc                                                                                        <  2:288445/140‑1 (MQ=255)                                                cgcgGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGccc                                                                                        >  1:206992/1‑136 (MQ=255)                                                cgcgGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGccc                                                                                        <  2:206992/136‑1 (MQ=255)                                                cgcgGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCgctg                                                                                    >  1:749795/1‑140 (MQ=255)                                                          gACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACAACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATAccgc                                                                          >  2:1010576/1‑140 (MQ=255)                                                               aGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGa                                                                                >  2:687267/1‑129 (MQ=255)                                                               aGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGa                                                                                <  1:687267/129‑1 (MQ=255)                                                               aGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCgaga                                                                     >  1:2938/1‑140 (MQ=255)                                                               aGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCgaga                                                                     >  2:305899/1‑140 (MQ=255)                                                                 ggATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAAt                                                                   >  2:630879/1‑140 (MQ=255)                                                                 ggATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAAt                                                                   >  1:839090/1‑140 (MQ=255)                                                                       tGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATgccgcc                                                             <  1:615998/140‑1 (MQ=255)                                                                        gACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGccgccc                                                            >  2:39982/1‑140 (MQ=255)                                                                         gcgtgcTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGccgcccg                                                           >  2:307469/6‑140 (MQ=255)                                                                         aCGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGccgcccg                                                           <  1:765935/140‑1 (MQ=255)                                                                         aCGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGccgcccg                                                           <  1:39982/140‑1 (MQ=255)                                                                           ggCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATAccgccg                                                                        <  2:454539/125‑1 (MQ=255)                                                                           ggCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATAccgccg                                                                        >  1:454539/1‑125 (MQ=255)                                                                              cTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATgg                                                      <  1:134423/140‑1 (MQ=255)                                                                               tttGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGt                                                     >  2:400109/1‑140 (MQ=255)                                                                               tttGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGt                                                     >  2:726302/1‑140 (MQ=255)                                                                                    ggttcaccTCAACTTTTTCGCGAAAGCGTTTATTGGTACG‑CGCCGAACGGCCAAGGCTGGAGGTCGGTTTAAGCGCCATATGGGAATTAATTTGCTGCCCGCGGCGGATACCGCCGAGAATGCCGCCCGCATGGTTGctc                                                <  2:560994/137‑1 (MQ=255)                                                                                         atcTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGATGCTCTGGaa                                           >  1:560994/1‑140 (MQ=255)                                                                                           ctcAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAAc                                         >  1:1054276/1‑140 (MQ=255)                                                                                            tcAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAAcc                                        <  2:523548/140‑1 (MQ=255)                                                                                            tcAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAAcc                                        <  1:1010576/140‑1 (MQ=255)                                                                                                cttcttCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCt                                    <  1:630879/140‑1 (MQ=255)                                                                                                     tcGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGt                               >  1:231689/1‑140 (MQ=255)                                                                                                      cGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTg                              <  2:1054276/140‑1 (MQ=255)                                                                                                               ggTTAATGGTACGACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATcg                     <  1:400109/140‑1 (MQ=255)                                                                                                                           gACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTg         >  2:579832/1‑140 (MQ=255)                                                                                                                           gACCCGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTg         >  2:101852/1‑140 (MQ=255)                                                                                                                              ccGGCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCcg      <  2:2938/140‑1 (MQ=255)                                                                                                                                 gCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCgcgc   >  1:847878/1‑140 (MQ=255)                                                                                                                                 gCACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCgcgc   >  2:977638/1‑140 (MQ=255)                                                                                                                                  cACGG‑CAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCa  >  1:68193/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                       GTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGG‑TAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCA  >  NC_000913/2446445‑2446713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑