Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,588,160	A→C	D189A (GAC→GCC)	acrD →	multidrug efflux pump RND permease AcrD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,588,160	0	A	C	100.0%	264.0 / NA	75	D189A (GAC→GCC)	acrD	multidrug efflux pump RND permease AcrD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (41/34);  total (41/34)

TCTACCGATGGTTCGATGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTTGGTGGTACACCTTCCGTCGATAAGCAGGCGCTCAACGCC  >  NC_000913/2588024‑2588290                                                                                                                                         |                                                                                                                                   tctACCGATGGTTCGATGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGcccc                                                                                                                                 >  1:540148/1‑140 (MQ=255)             tCGATGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCaa                                                                                                                     >  2:22370/1‑140 (MQ=255)             tCGATGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCaa                                                                                                                     >  1:849298/1‑140 (MQ=255)               gATGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACa                                                                                                                   >  1:15929/1‑140 (MQ=255)               gATGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACa                                                                                                                   >  1:29455/1‑140 (MQ=255)                aTGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAg                                                                                                                  >  2:887458/1‑140 (MQ=255)                aTGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAg                                                                                                                  <  1:371824/140‑1 (MQ=255)                    aTAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTc                                                                                                              >  1:326313/1‑140 (MQ=255)                      aaaCAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCa                                                                                                            >  1:80360/1‑140 (MQ=255)                          aGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATg                                                                                                        <  2:326313/140‑1 (MQ=255)                            gATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGAc                                                                                                      >  1:935126/1‑140 (MQ=255)                             atatTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACg                                                                                                     >  1:1130794/1‑140 (MQ=255)                              tatTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGGGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACgg                                                                                                    >  2:154386/1‑140 (MQ=255)                              tatTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACgg                                                                                                    >  1:219002/1‑140 (MQ=255)                              tatTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACgg                                                                                                    >  1:178990/1‑140 (MQ=255)                              tatTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATAGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCaa                                                                                                                     >  2:455750/1‑123 (MQ=255)                              tatTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATAGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCaa                                                                                                                     <  1:455750/123‑1 (MQ=255)                                ttGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCt                                                                                                  <  1:730838/140‑1 (MQ=255)                                 tGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGAt                                                                                                         <  1:861754/132‑1 (MQ=255)                                 tGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGAt                                                                                                         >  2:861754/1‑132 (MQ=255)                                 tGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTa                                                                                                 <  1:309628/140‑1 (MQ=255)                                 tGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTa                                                                                                 >  1:1064555/1‑140 (MQ=255)                                  gCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATg                                                                                                        <  2:634299/132‑1 (MQ=255)                                  gCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATg                                                                                                        >  1:634299/1‑132 (MQ=255)                                  gCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTaa                                                                                                >  1:1086416/1‑140 (MQ=255)                                  gCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTaa                                                                                                <  2:1130794/140‑1 (MQ=255)                                  gCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTaa                                                                                                >  1:360549/1‑140 (MQ=255)                                      aTTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGAt                                                                                            >  1:396691/1‑140 (MQ=255)                                      aTTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGAt                                                                                            >  2:393926/1‑140 (MQ=255)                                         aTGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTc                                                                                         >  2:997531/1‑140 (MQ=255)                                         aTGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTc                                                                                         >  2:529840/1‑140 (MQ=255)                                            ttGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTaa                                                                                                >  1:469558/1‑130 (MQ=255)                                            ttGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTaa                                                                                                <  2:469558/130‑1 (MQ=255)                                             tGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTg                                                                                     <  2:849298/140‑1 (MQ=255)                                               ccAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGAt                                                                                   <  2:540148/140‑1 (MQ=255)                                                 aGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGc                                                                                 <  2:644499/140‑1 (MQ=255)                                                   tAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCa                                                                               <  2:80360/140‑1 (MQ=255)                                                     atatTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCAtt                                                                             <  1:529840/140‑1 (MQ=255)                                                     atatTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCAtt                                                                             <  2:935126/140‑1 (MQ=255)                                                     atatTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCAtt                                                                             <  1:393926/140‑1 (MQ=255)                                                        ttCAGGACCCGTTAAGCCGCGTGAATGGGGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAg                                                                          <  1:154386/140‑1 (MQ=255)                                                           aGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTca                                                                       >  2:726601/1‑140 (MQ=255)                                                             gACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTcaca                                                                     >  2:755662/1‑140 (MQ=255)                                                              aCCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAg                                                                    <  1:1140146/140‑1 (MQ=255)                                                                 cGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAAc                                                                 >  2:1136628/1‑140 (MQ=255)                                                                 cGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAAc                                                                 >  1:111876/1‑140 (MQ=255)                                                                  gTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAAcg                                                                >  1:574938/1‑140 (MQ=255)                                                                    tAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAAcgcg                                                              <  2:360549/140‑1 (MQ=255)                                                                    tAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAAcgcg                                                              <  2:1086416/140‑1 (MQ=255)                                                                    tAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAAcgcg                                                              <  2:219002/140‑1 (MQ=255)                                                                       gCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAg                                                           >  1:950410/1‑140 (MQ=255)                                                                        ccGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGa                                                          <  2:1064555/140‑1 (MQ=255)                                                                         cgcgTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGAt                                                         <  2:574938/140‑1 (MQ=255)                                                                           cgTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGaa                                                                  <  2:210945/129‑1 (MQ=255)                                                                           cgTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGaa                                                                  >  1:210945/1‑129 (MQ=255)                                                                                aTGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGtt                                                  <  1:22370/140‑1 (MQ=255)                                                                                    cGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGc                                              <  1:1136628/140‑1 (MQ=255)                                                                                         gggATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACtt                                         >  1:281089/1‑140 (MQ=255)                                                                                          ggATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTtg                                        <  2:281089/140‑1 (MQ=255)                                                                                           gATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTtgg                                       <  2:15929/140‑1 (MQ=255)                                                                                               tCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTTggtggt                                   <  2:111876/140‑1 (MQ=255)                                                                                                       tATGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTTGGTGGTACACCTTc                           <  2:954054/140‑1 (MQ=255)                                                                                                         tGGTTCGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTTGGTGGTACACCTTCCg                         >  1:1102873/1‑140 (MQ=255)                                                                                                              cGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTTGGTGGTACACCtt                            <  2:1094730/132‑1 (MQ=255)                                                                                                              cGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTTGGTGGTACACCtt                            >  1:1094730/1‑132 (MQ=255)                                                                                                              cGCAATATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTTGGTGGTACACCTTCCGTCGAt                    >  1:568533/1‑140 (MQ=255)                                                                                                                 aaTATTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTTGGTGGTACACCTTCCGTCGATAAg                 <  1:726601/140‑1 (MQ=255)                                                                                                                   tatTCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTTGGTGGTACACCTTCCGTCGATAAGCa               >  1:506965/1‑140 (MQ=255)                                                                                                                      tCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTTGGTGGTACACCTTc                           >  1:1142284/1‑125 (MQ=255)                                                                                                                      tCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTTGGTGGTACACCTTc                           <  2:1142284/125‑1 (MQ=255)                                                                                                                      tCCATGCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTTGGTGGTACACCTTCCGTCGATAAGCAGgc            >  1:620207/1‑140 (MQ=255)                                                                                                                           gCGTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTTGGTGGTACACCTTCCGTCGATAAGCAGGCGCTCa       >  2:918891/1‑140 (MQ=255)                                                                                                                             gTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTTGGTGGTACACCTTCCGTCGATAAGCAGGCGCTCAAc     >  1:310392/1‑140 (MQ=255)                                                                                                                             gTATCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTTGGTGGTACACCTTCCGTCGATAAGCAGGCGCTCAAc     <  2:178990/140‑1 (MQ=255)                                                                                                                               aTCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTTGGTGGTACACCTTCCGTCGATAAGCAGGCGCTCAACGc   >  1:609368/1‑140 (MQ=255)                                                                                                                                tCTGGCTGGCCCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTTGGTGGTACACCTTCCGTCGATAAGCAGGCGCTCAACGcc  >  2:831163/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                   TCTACCGATGGTTCGATGGATAAACAGGATATTGCTGATTATGTTGCCAGTAATATTCAGGACCCGTTAAGCCGCGTGAATGGCGTCGGGGATATCGATGCCTATGGTTCGCAATATTCCATGCGTATCTGGCTGGACCCGGCGAAACTCAACAGTTTCCAGATGACGGCTAAAGATGTCACTGATGCCATTGAGTCACAGAACGCGCAGATTGCGGTTGGGCAACTTGGTGGTACACCTTCCGTCGATAAGCAGGCGCTCAACGCC  >  NC_000913/2588024‑2588290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑