Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,631,977	T→C	T187A (ACC→GCC)	guaA ←	GMP synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,631,977	0	T	C	100.0%	167.3 / NA	48	T187A (ACC→GCC)	guaA	GMP synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/25);  total (23/25)

CGCCTACCTGCTCGCGGATGCGAGCTACAGCATCGTCGATAATTTTCGCTGGCGTCCACAGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGG  >  NC_000913/2631857‑2632110                                                                                                                         |                                                                                                                                      cgCCTACCTGCTCGCGGATGCGAGCTACAGCATCGTCGATAATTTTCGCTGGCGTCCACAGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGCATGAGTCACTTCCGGGTgg                                                                                                                    <  2:1112249/140‑1 (MQ=255) cgCCTACCTGCTCGCGGATGCGAGCTACAGCATCGTCGATAATTTTCGCTGGCGTCCACAGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGCATGAGTCACTTCCGGGTgg                                                                                                                    <  1:540315/140‑1 (MQ=255)  gCCTACCTGCTCGCGGATGCGAGCTACAGCATCGTCGATAATTTTCGCTGGCGTCCACAGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGCATGAGTCACTTCCGGGTGGa                                                                                                                   >  2:879773/1‑140 (MQ=255)   ccTACCTGCTCGCGGATGCGAGCTACAGCATCGTCGATAATTTTCGCTGGCGTCCACAGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGCATGAGTCACTTCCGGGTGGaa                                                                                                                  <  2:446240/140‑1 (MQ=255)       ccTGCTCGCGGATGCGAGCTACAGCATCGTCGATAATTTTCGCTGGCGTCCACAGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGCATGAGTCACTTCCggg                                                                                                                       >  1:711887/1‑131 (MQ=255)       ccTGCTCGCGGATGCGAGCTACAGCATCGTCGATAATTTTCGCTGGCGTCCACAGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGCATGAGTCACTTCCggg                                                                                                                       <  2:711887/131‑1 (MQ=255)       ccTGCTCGCGGATGCGAGCTACAGCATCGTCGATAATTTTCGCTGGCGTCCACAGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGCATGAGTCACTTCCGGGTGGAACTGt                                                                                                              >  1:773468/1‑140 (MQ=255)        cTGCTCGCGGATGCGAGCTACAGCATCGTCGATAATTTTCGCTGGCGTCCACAGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGCATGAGTCACTTCCGGGTGGAACTGTa                                                                                                             <  2:686831/140‑1 (MQ=255)        cTGCTCGCGGATGCGAGCTACAGCATCGTCGATAATTTTCGCTGGCGTCCACAGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGCATGAGTCACTTCCGGGTGGAACTGTa                                                                                                             <  1:671120/140‑1 (MQ=255)        cTGCTCGCGGATGCGAGCTACAGCATCGTCGATAATTTTCGCTAGCGTCCACAGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGCATGAGTCACTTCCGGGTGGAACTGTa                                                                                                             <  2:951614/140‑1 (MQ=255)           cTCGCGGATGCGAGCTACAGCATCGTCGATAATTTTCGCTGGCGTCCACAGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGCATGAGTCACTTCCGGGTGGAACTGTACGc                                                                                                          >  2:477770/1‑140 (MQ=255)                     cGAGCTACAGCATCGTCGATAATTTTCGCTGGCGTCCACAGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGCATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCg                                                                                                >  1:432078/1‑140 (MQ=255)                        gCTACAGCATCGTCGATAATTTTCGCTGGCGTCCACAGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGCATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGctgtc                                                                                             >  1:723056/1‑136 (MQ=255)                              gCATCGTCGATAATTTTCGCTGGCGTCCACAGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGCATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGttttt                                                                                           >  2:580899/1‑136 (MQ=255)                              gCATCGTCGATAATTTTCGCTGGCGTCCACAGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGCATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGctttt                                                                                           <  1:580899/136‑1 (MQ=255)                              gCATCGTCGATAATTTTCGCTGGCGTCCACAGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGCATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTttcttc                                                                                       >  1:348481/1‑140 (MQ=255)                               cATCGTCGATAATTTTCGCTGGCGTCCACAGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGCATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCg                                                                                      <  1:157386/140‑1 (MQ=255)                                  cgtcgATAATTTTCGCTGGCGTCCACAGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGCATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGt                                                                                     <  1:92468/138‑1 (MQ=255)                                  cgtcgATAATTTTCGCTGGCGTCCACAGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGCATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGt                                                                                     >  2:92468/1‑138 (MQ=255)                                  cgtcgATAATTTTCGCTGGCGTCCACAGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGCATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTa                                                                                   >  2:466260/1‑140 (MQ=255)                                   gtcgATAATTTTCGCTGGCGTCCACAGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGCATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTttcttc                                                                                       >  1:642408/1‑135 (MQ=255)                                   gtcgATAATTTTCGCTGGCGTCCACAGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGCATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTttcttc                                                                                       <  2:642408/135‑1 (MQ=255)                                    tcgATAATTTTCGCTGGCGTCCACAGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGCATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGc                                                                                 <  2:432078/140‑1 (MQ=255)                                    tcgATAATTTTCGCTGGCGTCCACAGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGCATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGc                                                                                 >  1:403049/1‑140 (MQ=255)                                                   ggCGTCCACAGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGCATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACgg                                                                  <  2:773468/140‑1 (MQ=255)                                                           caGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGCATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCttt                                                          <  1:1147605/140‑1 (MQ=255)                                                              ggCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGCATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCgg                                                       >  2:824272/1‑140 (MQ=255)                                                              ggCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGCATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCg                                                        <  1:576679/139‑1 (MQ=255)                                                              ggCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGCATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCg                                                        >  2:576679/1‑139 (MQ=255)                                                               gCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGCATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGt                                                      <  2:348481/140‑1 (MQ=255)                                                                  tCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGCATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCt                                                   <  1:477770/140‑1 (MQ=255)                                                                   cacaCTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGCATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTg                                                  >  1:635946/1‑140 (MQ=255)                                                                       cTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGCATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTa                                              <  1:879773/140‑1 (MQ=255)                                                                          gCAGCTATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGCATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTgg                                                 <  1:788967/134‑1 (MQ=255)                                                                          gCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGCATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTgg                                                 >  2:788967/1‑134 (MQ=255)                                                                                  cACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGCATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGc                                                    <  2:320439/123‑1 (MQ=255)                                                                                  cACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGCATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGc                                                    >  1:320439/1‑123 (MQ=255)                                                                                         aaaaCGCTCCAGCATGCGCATACCCTGGCGGGCATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTcggacg                            >  2:998856/1‑140 (MQ=255)                                                                                            aCGCTCCAGCATGCGCATACCCTGGCGGGCATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGaa                         <  2:576277/140‑1 (MQ=255)                                                                                               cTCCAGCATGCGCATACCCTGGCGGGCATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAg                      >  2:66532/1‑140 (MQ=255)                                                                                                 ccAGCATGCGCATACCCTGGCGGGCATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCg                    >  2:584699/1‑140 (MQ=255)                                                                                                              aCCCTGGCGGGCATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGCTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGc       >  1:723494/1‑140 (MQ=255)                                                                                                               cccTGGCGGGCATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGcc      <  1:221990/140‑1 (MQ=255)                                                                                                                 cTGGCGGGCATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGt    <  2:635946/140‑1 (MQ=255)                                                                                                                  tGGCGGGCATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTg   >  2:118863/1‑140 (MQ=255)                                                                                                                  tGGCGGGCATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTg   >  1:40041/1‑140 (MQ=255)                                                                                                                   ggCGGGCATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTgg  <  2:723494/140‑1 (MQ=255)                                                                                                                   ggCGGGCATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTgg  <  1:824272/140‑1 (MQ=255)                                                                                                                         |                                                                                                                                      CGCCTACCTGCTCGCGGATGCGAGCTACAGCATCGTCGATAATTTTCGCTGGCGTCCACAGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGG  >  NC_000913/2631857‑2632110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑