Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	175,956	C→T	H284Y (CAC→TAC)	clcA →	chloride:H(+) antiporter ClcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	175,956	0	C	T	100.0%	256.9 / NA	72	H284Y (CAC→TAC)	clcA	chloride:H(+) antiporter ClcA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (41/31);  total (41/31)

ATTGACGTCGGTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCTCGGTATTATTTTTGGCATTTTCGGCCCTATTTTTAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGCACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCAG  >  NC_000913/175821‑176092                                                                                                                                        |                                                                                                                                         attgaCGTCGGTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCTCGGTATTATTTTTGGCATTTTCGGCCCTATTTTTAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGTAcgg                                                                                                                                      >  2:962492/1‑140 (MQ=255)   tgaCGTCGGTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCTCGGTATTATTTTTGGCATTTTCGGCCCTATTTTTAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGTAcggcg                                                                                                                                    <  1:962492/140‑1 (MQ=255)   tgaCGTCGGTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCTCGGTATTATTTTTGGCATTTTCGGCCCTATTTTTAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGTAcggcg                                                                                                                                    >  2:734781/1‑140 (MQ=255)    gaCGTCGGTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCTCGGTATTATTTTTGGCATTTTCGGCCCTATTTTTAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGTAcggcgg                                                                                                                                   <  2:253507/140‑1 (MQ=255)      cGTCGGTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCTCGGTATTATTTTTGGCATTTTCGGCCCTATTTTTAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGTACGGCGGCa                                                                                                                                 <  2:186060/140‑1 (MQ=255)        tCGGTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCTCGGTATTATTTTTGGCATTTTCGGCCCTATTTTTAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAat                                                                                                                               >  1:590153/1‑140 (MQ=255)          ggTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCTCGGTATTATTTTTGGCATTTTCGGCCCTATTTTTAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAatat                                                                                                                             <  1:734781/140‑1 (MQ=255)          ggTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCTCGGTATTATTTTTGGCATTTTCGGCCCTATTTTTAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAatat                                                                                                                             <  1:225393/140‑1 (MQ=255)          ggTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCTCGGTATTATTTTTGGCATTTTCGGCCCTATTTTTAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAatat                                                                                                                             <  2:590153/140‑1 (MQ=255)           gTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCTCGGTATTATTTTTGGCATTTTCGGCCCTATTTTTAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAATAtt                                                                                                                            >  2:472558/1‑140 (MQ=255)           gTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCTCGGTATTATTTTTGGCATTTTCGGCCCTATTTTTAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAATAtt                                                                                                                            >  2:803295/1‑140 (MQ=255)                   tCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCTCGGTATTATTTTTGGCATTTTCGGCCCTATTTTTAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATg                                                                                                                    >  1:516006/1‑140 (MQ=255)                   tCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCTCGGTATTATTTTTGGCATTTTCGGCCCTATTTTTAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATg                                                                                                                    >  2:309239/1‑140 (MQ=255)                                    tACGCTGTGGCTTTATCTGATCCTCGGTATTATTTTTGGCATTTTCGGCCCTATTTTTAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTg                                                                                                   <  1:803295/140‑1 (MQ=255)                                         tgtgGCTTTATCTGATCCTCGGTATTATTTTTGGCATTTTCGGCCCTATTTTTAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGAtt                                                                                              >  1:564908/1‑140 (MQ=255)                                         tgtgGCTTTATCTGATCCTCGGTATTATTTTTGGCATTTTCGGCCCTATTTTTAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGAtt                                                                                              >  1:1118768/1‑140 (MQ=255)                                          gtgGCTTTATCTGATCCTCGGTATTATTTTTGGCATTTTCGGCCCTATTTTTAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTg                                                                                             <  2:98383/140‑1 (MQ=255)                                                ttATCTGATCCTCGGTATTATTTTTGGCATTTTCGGCCCTATTTTTAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTc                                                                                       >  2:68469/1‑140 (MQ=255)                                                ttATCTGATCCTCGGTATTATTTTTGGCATTTTCGGCCCTATTTTTAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTc                                                                                       <  2:87019/140‑1 (MQ=255)                                                       aTCCTCGGTATTATTTTTGGCATTTTCGGCCCTATTTTTAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGc                                                                                                  >  1:456204/1‑122 (MQ=255)                                                       aTCCTCGGTATTATTTTTGGCATTTTCGGCCCTATTTTTAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGc                                                                                                  <  2:456204/122‑1 (MQ=255)                                                       aTCCTCGGTATTATTTTTGGCATTTTCGGCCCTATTTTTAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCtgtgtg                                                                                 >  1:496971/1‑139 (MQ=255)                                                       aTCCTCGGTATTATTTTTGGCATTTTCGGCCCTATTTTTAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCtgtgtg                                                                                 <  2:496971/139‑1 (MQ=255)                                                       aTCCTCGGTATTATTTTTGGCATTTTCGGCCCTATTTTTAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTgg                                                                                >  1:351897/1‑140 (MQ=255)                                                        tCCTCGGTATTATTTTTGGCATTTTCGGCCCTATTTTTAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGa                                                                               <  2:79570/140‑1 (MQ=255)                                                            cGGTATTATTTTTGGCATTTTCGGCCCTATTTTTAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGc                                                                           >  2:307651/1‑140 (MQ=255)                                                            cGGTATTATTTTTGGCATTTTCGGCCCTATTTTTAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGc                                                                           >  1:30168/1‑140 (MQ=255)                                                             ggTATTATTTTTGGCATTTTCGGCCCTATTTTTAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCt                                                                          >  1:1021068/1‑140 (MQ=255)                                                              gTATTATTTTTGGCATTTTCGGCCCTATTTTTAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTg                                                                         <  2:1118768/140‑1 (MQ=255)                                                                attattTTTGGCATTTTCGGCCCTATTTTTAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTggg                                                                       <  1:307651/140‑1 (MQ=255)                                                                 ttattTTTGGCATTTTCGGCCCTATTTTTAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTgggg                                                                      >  2:1082870/1‑140 (MQ=255)                                                                      tttGGCATTTTCGGCCCTATTTTTAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTtgt                                                                 <  1:68469/140‑1 (MQ=255)                                                                           cATTTTCGGCCCTATTTTTAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCAc                                                            <  2:607366/140‑1 (MQ=255)                                                                              tttCGGCCCTATTTTTAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAg                                                         >  1:958356/1‑140 (MQ=255)                                                                                    cccTATTTTTAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGt                                                   >  2:9348/1‑140 (MQ=255)                                                                                        aTTTTTAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCggg                                               >  1:2867/1‑140 (MQ=255)                                                                                           tttAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGggcgg                                            <  2:958356/140‑1 (MQ=255)                                                                                                  aaTGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGtttt                                     <  2:408930/140‑1 (MQ=255)                                                                                                   aTGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTa                                    >  1:1107019/1‑140 (MQ=255)                                                                                                   aTGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTa                                    >  2:744383/1‑140 (MQ=255)                                                                                                   aTGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTa                                    <  2:2867/140‑1 (MQ=255)                                                                                                   aTGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTa                                    >  2:1051535/1‑140 (MQ=255)                                                                                                   aTGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTa                                    >  1:532970/1‑140 (MQ=255)                                                                                                     gggTGCTGGGGATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAAc                                  <  1:505842/140‑1 (MQ=255)                                                                                                     gggTGCTGGGGATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAAc                                  <  2:516006/140‑1 (MQ=255)                                                                                                      ggTGCTGGGGATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAAcc                                 >  2:116453/1‑140 (MQ=255)                                                                                                      ggTGCTGGGGATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAAcc                                 >  1:45606/1‑140 (MQ=255)                                                                                                        tGCTGGGGATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTg                               >  2:481552/1‑140 (MQ=255)                                                                                                         gCTGGGGATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGa                              >  2:19665/1‑140 (MQ=255)                                                                                                         gCTGGGGATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGa                              <  2:532970/140‑1 (MQ=255)                                                                                                          cTGGGGATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGAt                             >  1:179649/1‑140 (MQ=255)                                                                                                             gggATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTcc                          >  1:4085/1‑140 (MQ=255)                                                                                                             gggATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTcc                          <  2:734288/140‑1 (MQ=255)                                                                                                              ggATGCAGGATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCt                         <  1:1082870/140‑1 (MQ=255)                                                                                                                aTGCAGGATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTAt                       >  2:1007718/1‑140 (MQ=255)                                                                                                                  gCAGGATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCg                     >  1:419988/1‑140 (MQ=255)                                                                                                                  gCAGGATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCg                     <  2:1021068/140‑1 (MQ=255)                                                                                                                  gCAGGATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCg                     >  1:500632/1‑140 (MQ=255)                                                                                                                     ggATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTa                  <  1:1007718/140‑1 (MQ=255)                                                                                                                      gATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTAc                 <  2:500632/140‑1 (MQ=255)                                                                                                                      gATTTGCTGCACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTAc                 >  2:450791/1‑140 (MQ=255)                                                                                                                            ctgcACCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCggg           <  2:1107019/140‑1 (MQ=255)                                                                                                                                cccGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAAt       <  2:359847/139‑1 (MQ=255)                                                                                                                                aCCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAAt       <  2:564908/140‑1 (MQ=255)                                                                                                                                aCCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAAt       >  2:508846/1‑140 (MQ=255)                                                                                                                                aCCGTGTGTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAAt       <  1:508846/140‑1 (MQ=255)                                                                                                                                    tgtgTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCa   >  2:890303/1‑140 (MQ=255)                                                                                                                                    tgtgTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCa   >  1:438998/1‑140 (MQ=255)                                                                                                                                    tgtgTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCa   >  1:352005/1‑140 (MQ=255)                                                                                                                                    tgtgTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCa   >  1:524229/1‑140 (MQ=255)                                                                                                                                    tgtgTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCa   >  1:994378/1‑140 (MQ=255)                                                                                                                                    tgtgTACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCa   >  1:661341/1‑140 (MQ=255)                                                                                                                                     gtgtACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCAg  <  2:661341/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         ATTGACGTCGGTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCTCGGTATTATTTTTGGCATTTTCGGCCCTATTTTTAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGCACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCAG  >  NC_000913/175821‑176092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑