Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,840,934	T→C	Y471H (TAT→CAT)	ascF →	beta‑glucoside specific PTS enzyme IIBC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,840,934	0	T	C	100.0%	169.3 / NA	49	Y471H (TAT→CAT)	ascF	beta‑glucoside specific PTS enzyme IIBC component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/21);  total (28/21)

CGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCAGTCTGAAGGTGCGTTCCGTGAAGGTGA  >  NC_000913/2840798‑2841069                                                                                                                                         |                                                                                                                                        cGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATc                                                                                                                                      <  1:181077/140‑1 (MQ=255)  gATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCa                                                                                                                                     <  2:534405/140‑1 (MQ=255)    tCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCaga                                                                                                                                   <  2:1096882/140‑1 (MQ=255)      cGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGc                                                                                                                                 >  2:354821/1‑140 (MQ=255)        gCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGt                                                                                                                               >  1:8254/1‑140 (MQ=255)             tCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAac                                                                                                                          >  1:1024852/1‑140 (MQ=255)             tCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAac                                                                                                                          <  1:540252/140‑1 (MQ=255)                  tGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAaccgacc                                                                                                                     >  1:355896/1‑140 (MQ=255)                        tCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGcc                                                                                                               >  2:373648/1‑140 (MQ=255)                          gTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCaa                                                                                                             >  2:330485/1‑140 (MQ=255)                           tCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCaa                                                                                                             <  1:225884/139‑1 (MQ=255)                           tCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCaa                                                                                                             >  2:225884/1‑139 (MQ=255)                             tGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAaaga                                                                                                          >  1:994048/1‑140 (MQ=255)                               ggTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAaagaag                                                                                                        <  2:994048/140‑1 (MQ=255)                                 tgtTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGaagta                                                                                                      <  1:330485/140‑1 (MQ=255)                                    tCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGaagtaagt                                                                                                   <  1:810100/140‑1 (MQ=255)                                     cgcgGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTc                                                                                                  <  2:8254/140‑1 (MQ=255)                                     cgcgGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTc                                                                                                  >  1:370881/1‑140 (MQ=255)                                         gTCATGGCGCTGGCGGGGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGGTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCCAAGAAGTAAGTCTTaa                                                                                              >  2:415455/1‑140 (MQ=255)                                               gcgcTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAgg                                                                                        <  2:1024852/140‑1 (MQ=255)                                                  cTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATg                                                                                     >  2:578411/1‑140 (MQ=255)                                                          ggtgCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTc                                                                             <  2:355896/140‑1 (MQ=255)                                                                 tCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTAttt                                                                      >  2:518413/1‑140 (MQ=255)                                                                      tATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGa                                                                 <  1:713770/140‑1 (MQ=255)                                                                           tCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGtt                                                            >  2:156745/1‑140 (MQ=255)                                                                               acTGTTGCTCGGCTTTCAGGATATTCCTGTTGAGGAAGCGGCTGCACCGGCGCGAACGCATCGGAGCGTACAACCGACCGTCGCCAAAGGAGCGAGTCTTAATTGAGGATGAAAATGTCAGTCTTTGGAGACAGtttttt                                                        >  2:520594/1‑140 (MQ=255)                                                                                   ttGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATgg                                                    >  1:1026271/1‑140 (MQ=255)                                                                                   ttGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATgg                                                    >  1:325548/1‑140 (MQ=255)                                                                                    tGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGCAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGAATTTCCAGAAAGTTTTTTATggg                                                   >  2:757800/1‑140 (MQ=255)                                                                                    tGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATggg                                                   >  2:864484/1‑140 (MQ=255)                                                                                      cTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGggc                                                 >  2:546025/1‑140 (MQ=255)                                                                                      cTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGggc                                                 >  1:900436/1‑140 (MQ=255)                                                                                           cTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGgcgc                                            >  1:947188/1‑140 (MQ=255)                                                                                                aGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTg                                       <  1:546025/140‑1 (MQ=255)                                                                                                aGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTg                                       <  1:848854/140‑1 (MQ=255)                                                                                                     atTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTgccgcc                                  >  2:542533/1‑140 (MQ=255)                                                                                                     atTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTgccgcc                                  <  2:947188/140‑1 (MQ=255)                                                                                                     atTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTgccgcc                                  >  2:461765/1‑140 (MQ=255)                                                                                                        ccTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAAc                               <  1:646952/140‑1 (MQ=255)                                                                                                         cTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAAcc                              >  2:929860/1‑140 (MQ=255)                                                                                                            ttGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCAGt                           >  2:539462/1‑140 (MQ=255)                                                                                                              gAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAAc                               >  2:123946/1‑134 (MQ=255)                                                                                                              gAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAAc                               <  1:123946/134‑1 (MQ=255)                                                                                                               aGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCAGTCTg                        >  1:787422/1‑140 (MQ=255)                                                                                                                    gCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCAGTCTGAAGGt                   >  1:588882/1‑140 (MQ=255)                                                                                                                    gCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCAGTCTGAAGGt                   <  1:373648/140‑1 (MQ=255)                                                                                                                    gCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCAGTCTGAAGGt                   <  1:415455/140‑1 (MQ=255)                                                                                                                      ggCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCAGTCTGAAGGTGc                 >  2:22968/1‑140 (MQ=255)                                                                                                                             cAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCAGTCTGAAGGTGCGTTCCGt          <  2:370881/140‑1 (MQ=255)                                                                                                                                gcgcGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCAGTCTGAAGGTGCGTTCCGTGaa       >  1:551419/1‑140 (MQ=255)                                                                                                                                gcgcGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCAGTCTGAAGGTGCGTTCCGTGaa       <  1:864484/140‑1 (MQ=255)                                                                                                                                     aaaGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCAGTCTGAAGGTGCGTTCCGTGAAGGTGa  >  2:472086/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        CGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCAGTCTGAAGGTGCGTTCCGTGAAGGTGA  >  NC_000913/2840798‑2841069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑