Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,682,157	(C)7→6	coding (589/3282 nt)	yphG ←	DUF5107 domain‑containing protein YphG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,682,151	0	C	.	100.0%	123.5 / NA	30	coding (595/3282 nt)	yphG	DUF5107 domain‑containing protein YphG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (16/14);  total (16/14)

TTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCA  >  NC_000913/2682012‑2682284                                                                                                                                            |                                                                                                                                      ttaaggCGAAAAATGCCCCCTCCGGCGGAGTGGTCCTCTTGTTAGTAAGGCCGGGTAGGAGGGGGGAAGGGGGGGGCGGCCCTTTGCCCGTGATAAAACCCCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTc                                                                                                                                       <  2:81106/135‑1 (MQ=255)                        gggggAGTAGTCCTTTTTGTAGTAGGTGCGGGTAGGGAGGGGAAAGGCGGAGCCGCCCCTTTTGCCGTAACAAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTT‑CCCCCCTTTCACTGCCGGGTTGGc                                                                                                               <  2:873485/137‑1 (MQ=255)                          cGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTT‑CCCCCCTTTCACTGCCGGGTTGGccc                                                                                                             >  1:527387/1‑140 (MQ=255)                                         tGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTT‑CCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGAc                                                                                              >  1:352566/1‑140 (MQ=255)                                         tGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTT‑CCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGAc                                                                                              >  2:553084/1‑140 (MQ=255)                                                   gcgggTAGCGAGGGGAAAGGGGGAGGCGCCCCGTTCGCCGTAATCAACCCCCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTT‑CCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCggcgtggc                                                                                    <  2:599284/137‑1 (MQ=255)                                                     cGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTT‑CCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCggcgtggcgt                                                                                  >  1:27722/1‑140 (MQ=255)                                                      ggTAGCGAGGGGAAAGGGGGAGCCGGCCCTTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTT‑CCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGtt                                                                                 <  2:92834/140‑1 (MQ=255)                                                       gTAGCGAGGGGGAAGGCGGAGGGGGCCCTTTTGCCGTGAAAAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTT‑CCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTc                                                                                <  2:527387/140‑1 (MQ=255)                                                            gATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTT‑CCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGtt                                                                           >  1:599284/1‑140 (MQ=255)                                                                gggAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTT‑CCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAg                                                                       >  1:92834/1‑140 (MQ=255)                                                                          agaCGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTT‑CCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTgg                                                             >  1:275058/1‑140 (MQ=255)                                                                            ccgccccGTTTGCCGTAAACAACCCCCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTT‑CCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCg                                                           <  1:463506/136‑1 (MQ=255)                                                                               ccccTTTTCCCGTAATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTT‑CCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGAtt                                                        <  1:589996/139‑1 (MQ=255)                                                                                    tttGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTT‑CCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAg                                                   >  1:277304/1‑140 (MQ=255)                                                                                           tGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTT‑CCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCgccgcc                                            <  2:352566/140‑1 (MQ=255)                                                                                                      cgccgTTACATCCGGCGGGAAGACGCTCTGATGCCCTT‑CCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGgcg                                 <  1:11688/140‑1 (MQ=255)                                                                                                       gccgTTACATCCGGCGGGAAGACGCTCTGATGCCCTT‑CCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGgcgc                                >  1:269094/1‑140 (MQ=255)                                                                                                        ccgTTACATCCGGCGGGAAGACGCTCTGATGCCCTT‑CCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCa                               >  2:230674/1‑140 (MQ=255)                                                                                                        ccgTTACATCCGGCGGGAAGACGCTCTGATGCCCTT‑CCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCa                               >  1:302513/1‑140 (MQ=255)                                                                                                                   ggcggGAAGACGCTCTGATGCCCTT‑CCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCt                    <  1:553084/140‑1 (MQ=255)                                                                                                                     cggGAAGACGCTCTGATGCCCTT‑CCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGt                  >  2:121243/1‑140 (MQ=255)                                                                                                                     cggGAAGACGCTCTGATGCCCTT‑CCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGt                  >  2:584515/1‑140 (MQ=255)                                                                                                                        gAAGACGCTCTGATGCCCTT‑CCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGtcat               <  2:275058/140‑1 (MQ=255)                                                                                                                        gAAGACGCTCTGATGCCCTT‑CCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGtcat               <  2:727019/140‑1 (MQ=255)                                                                                                                             cGCTCTGATGCCCTT‑CCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCt          <  2:461977/140‑1 (MQ=255)                                                                                                                                tctGATGCCCTT‑CCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTa       >  1:331922/1‑140 (MQ=255)                                                                                                                                  tGATGCCCTT‑CCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGtcat               <  2:476568/130‑1 (MQ=255)                                                                                                                                  tGATGCCCTT‑CCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGtcat               >  1:476568/1‑130 (MQ=255)                                                                                                                                  tGATGCCCTT‑CCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTaaa     <  2:331922/140‑1 (MQ=255)                                                                                                                                     tGCCCTT‑CCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCa  >  2:561186/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                      TTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCA  >  NC_000913/2682012‑2682284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑