Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,977,134	Δ1 bp	coding (857/1251 nt)	wzxE →	lipid III flippase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,977,131	0	A	.	100.0%	245.8 / NA	58	coding (854/1251 nt)	wzxE	lipid III flippase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (34/24);  total (34/24)

GTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTCCGATGCCTACCTGCAATTTATTACGGCATCGTTCAGCGTATATTTGCTGCCCACGTTGTCGCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGC  >  NC_000913/3976993‑3977263                                                                                                                                           |                                                                                                                                     gTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTCCGATGCCTACCTGCAATTTATTACGGCATCGTTCAGCGTATATTTGCTGCCCACGTTGTCGCGGCTAACGGaa                                                                                                                                     >  2:206193/1‑140 (MQ=255)    aaCTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGACAAGGGGTGAGCAGTATTTCCGATGCCTACCTGCAATTTATTACGGCATCGTTCAGCGTATATTTGCTGCCCACGTTGTCGCGGCTAACGGaaagc                                                                                                                                  <  1:262246/140‑3 (MQ=255)         ctgGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTCCGATGCCTACCTGCAATTTATTACGGCATCGTTCAGCGTATATTTGCTGCCCACGTTGTCGCGGCTAACGG‑AAAGCGCGat                                                                                                                            <  2:551009/140‑1 (MQ=255)           ggcggcGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTCCGATGCCTACCTGCAATTTATTACGGCATCGTTCAGCGTATATTTGCTGCCCACGTTGTCGCGGCTAACGG‑AAAGCGCGatat                                                                                                                          <  2:256349/140‑1 (MQ=255)           ggcggcGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTCCGATGCCTACCTGCAATTTATTACGGCATCGTTCAGCGTATATTTGCTGCCCACGTTGTCGCGGCTAACGG‑AAAGCGCGatat                                                                                                                          <  1:550435/140‑1 (MQ=255)            gcggcgCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTCCGATGCCTACCTGCAATTTATTACGGCATCGTTCAGCGTATATTTGCTGCCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATc                                                                                                                         >  2:395071/1‑140 (MQ=255)                 gcAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTCCGATGCCTACCTGCAATTTATTACGGCATCGTTCAGCGTATATTTGCTGCCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCg                                                                                                                    >  1:377075/1‑140 (MQ=255)                   aGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTCCGATGCCTCCCTGCAATTTATTACGGCATCGTTCAGCGTATATTTGCTGCCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCggg                                                                                                                  <  2:922962/140‑1 (MQ=255)                   aGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTCCGATGCCTACCTGCAATTTATTACGGCATCGTTCAGCGTATATTTGCTGCCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCggg                                                                                                                  <  1:422819/140‑1 (MQ=255)                    gTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTCCGATGCCTACCTGCAATTTATTACGGCATCGTTCAGCGTATATTTGCTGCCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCGGGa                                                                                                                 <  1:206193/140‑1 (MQ=255)                     tataGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTCCGATGCCTACCTGCAATTTATTACGGCATCGTTCAGCGTATATTTGCTGCCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCGGGaa                                                                                                                <  2:457328/140‑1 (MQ=255)                     tataGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTCCGATGCCTACCTGCAATTTATTACGGCATCGTTCAGCGTATATTTGCTGCCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCGGGaa                                                                                                                <  2:319968/140‑1 (MQ=255)                          cTGGGATGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTCCGATGCCTACCTGCAATTTATTACGGCATCGTTCAGCGTATATTTGCTGCCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCGGGAAGTGGt                                                                                                           >  2:520962/1‑140 (MQ=255)                            gggATGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTCCGATGCCTACCTGCAATTTATTACGGCATCGTTCAGCGTATATTTGCTGCCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCGGGAAGTGGTTa                                                                                                         >  1:398892/1‑140 (MQ=255)                               aTGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTCCGATGCCTACCTGCAATTTATTACGGCATCGTTCAGCGTATATTTGCTGCCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCGGGAAGTGGTTAAAt                                                                                                      >  1:780597/1‑140 (MQ=255)                               aTGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTCCGATGCCTACCTGCAATTTATTACGGCATCGTTCAGCGTATATTTGCTGCCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCGGGAAGTGGTTAAAt                                                                                                      >  2:297788/1‑140 (MQ=255)                               aTGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTCCGATGCCTACCTGCAATTTATTACGGCATCGTTCAGCGTATATTTGCTGCCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCGGGAAGTGGTTAAAt                                                                                                      >  2:592987/1‑140 (MQ=255)                               aTGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTCCGATGCCTACCTGCAATTTATTACGGCATCGTTCAGCGTATATTTGCTGCCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCGGGAAGTGGTTAAAt                                                                                                      >  1:393387/1‑140 (MQ=255)                                  aGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTCCGATGCCTACCTGCAATTTATTACGGCATCGTTCAGCGTATATTTGCTGCCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCGGGaa                                                                                                                >  1:172440/1‑127 (MQ=255)                                  aGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTCCGATGCCTACCTGCAATTTATTACGGCATCGTTCAGCGTATATTTGCTGCCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCGGGaa                                                                                                                <  2:172440/127‑1 (MQ=255)                                    gTGGGGATCTGGCAAGGGGTGAGCAGTATTTCCGATGCCTACCTGCAATTTATTACGGCATCGTTCAGCGTATATTTGCTGCCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTg                                                                                                 >  1:182758/1‑140 (MQ=255)                                          aTCTGGCAAGGGGTGAGCAGTATTTCCGATGCCTACCTGCAATTTATTACGGCATCGTTCAGCGTATATTTGCTGCCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTc                                                                                           <  2:377075/140‑1 (MQ=255)                                                  aGGGGTGAGCAGTATTTCCGATGCCTACCTGCAATTTATTACGGCATCGTTCAGCGTATATTTGCTGCCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTAcc                                                                                   <  1:520962/140‑1 (MQ=255)                                                   ggggTGAGCAGTATTTCCGATGCCTACCTGCAATTTATTACGGCATCGTTCAGCGTATATTTGCTGCCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCg                                                                                  >  2:605023/1‑140 (MQ=255)                                                     ggTGAGCAGTATTTCCGATGCCTACCTGCAATTTATTACGGCATCGTTCAGCGTATATTTGCTGCCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGc                                                                                >  2:448079/1‑140 (MQ=255)                                                              tATTTCCGATGCCTACCTGCAATTTATTACGGCATCGTTCAGCGTATATTTGCTGCCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTggcggc                                                                       >  2:881496/1‑140 (MQ=255)                                                              tATTTCCGATGCCTACCTGCAATTTATTACGGCATCGTTCAGCGTATATTTGCTGCCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTggcggc                                                                       >  1:313403/1‑140 (MQ=255)                                                              tATTTCCGATGCCTACCTGCAATTTATTACGGCATCGTTCAGCGTATATTTGCTGCCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTggcggc                                                                       >  1:377886/1‑140 (MQ=255)                                                                        gCCTACCTGCAATTTATTACGGCATCGTTCAGCGTATATTTGCTGCCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTggcggcggc                                                                    >  2:602689/1‑133 (MQ=255)                                                                        gCCTACCTGCAATTTATTACGGCATCGTTCAGCGTATATTTGCTGCCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTggcggcggc                                                                    <  1:602689/133‑1 (MQ=255)                                                                        gCCTACCTGCAATTTATTACGGCATCGTTCAGCGTATATTTGCTGCCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGtttt                                                             >  1:85992/1‑140 (MQ=255)                                                                        gCCTACCTGCAATTTATTACGGCATCGTTCAGCGTATATTTGCTGCCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGtttt                                                             >  2:516692/1‑140 (MQ=255)                                                                             ccTGCAATTTATTACGGCATCGTTCAGCGTATATTTGCTGCCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGt                                                        <  1:605023/140‑1 (MQ=255)                                                                              cTGCAATTTATTACGGCATCGTTCAGCGTATATTTGCTGCCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGTc                                                       <  2:377886/140‑1 (MQ=255)                                                                                    tttATTACGGCATCGTTCAGCGTATATTTGCTGCCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGTCTGgctg                                                 <  1:395071/140‑1 (MQ=255)                                                                                      tattaCGGCATCGTTCAGCGTATATTTGCTGCCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGTCTGgctgct                                               >  1:59342/1‑140 (MQ=255)                                                                                      tattaCGGCATCGTTCAGCGTATATTTGCTGCCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGTCTGgctgct                                               >  1:575212/1‑140 (MQ=255)                                                                                         taCGGCATCGTTCAGCGTATATTTGCTGCCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGTCTGGCTGCTGCg                                            <  2:85992/140‑1 (MQ=255)                                                                                              cATCGTTCAGCGTATATTTGCTGCCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGTCTGGCTGCTGCGTGAtt                                       >  2:38239/1‑140 (MQ=255)                                                                                                    tCAGCGTATATTTGCTGCCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTa                                 <  1:38239/140‑1 (MQ=255)                                                                                                    tCAGCGTATATTTGCTGCCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTa                                 <  2:398892/140‑1 (MQ=255)                                                                                                       gCGTATATTTGCTGCCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCt                              >  2:827379/1‑140 (MQ=255)                                                                                                         gTATATTTGCTGCCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTgg                            <  1:297788/140‑1 (MQ=255)                                                                                                          tataTTTGCTGCCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCGCCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGgc                           <  2:757017/140‑1 (MQ=255)                                                                                                          tataTTTGCTGCCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGgc                           >  1:65963/1‑140 (MQ=255)                                                                                                            tatTTGCTGCCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGgctg                         >  2:227639/1‑140 (MQ=255)                                                                                                                  ctgcCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTg                             <  1:184382/130‑1 (MQ=255)                                                                                                                  ctgcCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTg                             >  2:184382/1‑130 (MQ=255)                                                                                                                  ctgcCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCtgttg                   <  1:516692/140‑1 (MQ=255)                                                                                                                  ctgcCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCtgttg                   >  1:580145/1‑140 (MQ=255)                                                                                                                  ctgcCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCtgttg                   >  2:859280/1‑140 (MQ=255)                                                                                                                  ctgcCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCtgttg                   >  2:843626/1‑140 (MQ=255)                                                                                                                  ctgcCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCtgttg                   >  2:246015/1‑140 (MQ=255)                                                                                                                    gcCCACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTc                 >  2:611484/1‑140 (MQ=255)                                                                                                                     cccACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCg                >  2:485173/1‑140 (MQ=255)                                                                                                                     cccACGTTGTCGCGGCTAACGG‑AAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGGTTTACCGTCTGGCTGCTGCGTGGTTTTGCTATCTGGCTGCTGTTGTCg                >  2:593426/1‑140 (MQ=255)                                                                                                                               cgcgGCTAACGG‑AAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTa      >  1:654020/1‑140 (MQ=255)                                                                                                                                  ggCTAACGG‑AAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCg   <  2:59342/140‑1 (MQ=255)                                                                                                                                  ggCTAACGG‑AAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCg   <  1:592987/140‑1 (MQ=255)                                                                                                                                   gCTAACGG‑AAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGc  <  1:859280/140‑1 (MQ=255)                                                                                                                                   gCTAACGG‑AAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGGGGCGGCGGCGAGGTTTACCTGCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGc  >  2:287239/1‑140 (MQ=255)                                                                                                                                           |                                                                                                                                     GTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTCCGATGCCTACCTGCAATTTATTACGGCATCGTTCAGCGTATATTTGCTGCCCACGTTGTCGCGGCTAACGGAAAAGCGCGATATCACCCGGGAAGTGGTTAAATCGCTGAAATTCGTCTTACCGGCAGTGGCGGCGGCGAGTTTTACCGTCTGGCTGCTGCGTGATTTTGCTATCTGGCTGCTGTTGTCGAATAAATTTACCGC  >  NC_000913/3976993‑3977263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑