Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,084,255	Δ1 bp	coding (1568/3051 nt)	fdoG ←	formate dehydrogenase O subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,084,252	0	C	.	100.0%	149.4 / NA	36	coding (1571/3051 nt)	fdoG	formate dehydrogenase O subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (15/21);  total (15/21)

ATCACTTTGTTTTTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAGCCCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACGAAGAATTTCGGGTAGTTGCCCCAGTAGTTTACCTGGCCTTCCAGCAGCGGTTTTGGCGTGTTGGCGGTAAGGTAGGT  >  NC_000913/4084114‑4084385                                                                                                                                           |                                                                                                                                      aTCACTTTGTTTTTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAGcc                                                                                                                                      <  2:430023/140‑1 (MQ=255)    aCTTTGTTTTTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAGcccag                                                                                                                                   >  2:362488/1‑138 (MQ=255)                ttCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTccgcc                                                                                                                      <  2:412827/140‑1 (MQ=255)                         gAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTa                                                                                                             <  1:406188/140‑1 (MQ=255)                           ggCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATc                                                                                                           <  1:289062/140‑1 (MQ=255)                            gCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCa                                                                                                          <  2:507161/140‑1 (MQ=255)                              aaCAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCAcc                                                                                                        >  2:879314/1‑140 (MQ=255)                               aCAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCa                                                                                                       >  1:244775/1‑140 (MQ=255)                               aCAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCa                                                                                                       >  1:586497/1‑140 (MQ=255)                                       aaaGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGc                                                                                               <  2:586497/140‑1 (MQ=255)                                       aaaGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGc                                                                                               <  2:244775/140‑1 (MQ=255)                                          gCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCtt                                                                                            <  1:362488/140‑1 (MQ=255)                                          gCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCtt                                                                                            <  1:17848/140‑1 (MQ=255)                                             cTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTtcat                                                                                         >  1:369608/1‑140 (MQ=255)                                                    atatAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATagag                                                                                  <  1:293754/140‑1 (MQ=255)                                                          ccATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCtt                                                                                            >  1:230390/1‑124 (MQ=255)                                                          ccATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCtt                                                                                            <  2:230390/124‑1 (MQ=255)                                                          ccATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACgaa                                                                             <  1:410943/139‑1 (MQ=255)                                                          ccATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACgaa                                                                             >  2:410943/1‑139 (MQ=255)                                                              tGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACGAAGAAtt                                                                        >  1:47667/1‑140 (MQ=255)                                                                    tGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACGAAGAATTTCGGGt                                                                  >  1:917929/1‑140 (MQ=255)                                                                          ctTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACGAAGAATTTCGGGTAGTTGc                                                            >  1:504436/1‑140 (MQ=255)                                                                           tttCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACGAAGAAtt                                                                        >  1:376104/1‑127 (MQ=255)                                                                           tttCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACGAAGAAtt                                                                        <  2:376104/127‑1 (MQ=255)                                                                           tttCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACGAAGAATTTCGGGTAGTTg                                                             >  2:664357/1‑138 (MQ=255)                                                                           tttCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACGAAGAATTTCGGGTAGTTg                                                             <  1:664357/138‑1 (MQ=255)                                                                           tttCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACGAAGAATTTCGGGTAGTTGcc                                                           >  2:384904/1‑140 (MQ=255)                                                                             tcatcaTCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACGAAGAATTTCGGGTAGTTGcccc                                                         <  1:879314/140‑1 (MQ=255)                                                                             tcatcaTCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACGAAGAATTTCGGGTAGTTGcccc                                                         <  1:245127/140‑1 (MQ=255)                                                                             tcatcaTCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACGAAGAATTTCGGGTAGTTGcccc                                                         <  2:369608/140‑1 (MQ=255)                                                                                tcatcTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACGAAGAATTTCGGGTAGTTGCCCCagt                                                      >  2:721585/1‑140 (MQ=255)                                                                                  atcTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACGAAGAATTTCGGGTAGTTGCCCCagtag                                                    <  1:721585/140‑1 (MQ=255)                                                                                         aGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACGAAGAATTTCGGGTAGTTGCCCCAGTAGTTTACCt                                             <  2:731560/140‑1 (MQ=255)                                                                                         aGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACGAAGAATTTCGGGTAGTTGCCCCAATAGTTTACCt                                             <  2:504436/140‑1 (MQ=255)                                                                                            aTTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACGAAGAATTTCGGGTAGTTGCCCCAGTAGTTTACCTGGc                                          >  2:18591/1‑140 (MQ=255)                                                                                                                  aTCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACGAAGAATTTCGGGTAGTTGCCCCAGTAGTTTACCTGGCCTTCCAGCAGCGGTTTTGGCgt                    >  2:766683/1‑140 (MQ=255)                                                                                                                    cccACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACGAAGAATTTCGGGTAGTTGCCCCAGTAGTTTACCTGGCCTTCCAGCAGCGGTTTTGGCgtgt                  <  1:766683/140‑1 (MQ=255)                                                                                                                        cTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACGAAGAATTTCGGGTAGTTGCCCCAGTAGTTTACCTGGCCTTCCAGCAGCGGTTTTGGCGTGTTGGc              >  2:421566/1‑140 (MQ=255)                                                                                                                                aCCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACGAAGAATTTCGGGTAGTTGCCCCAGTAGTTTACCTGGCCTTCCAGCAGCGGTTTTGGCGTGTTGGCGGTAaggt      <  1:18591/140‑1 (MQ=255)                                                                                                                                    gTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACGAAGAATTTCGGGTAGTTGCCCCAGTAGTTTACCTGGCCTTCCAGCAGCGGTTTTGGCGTGTTGGCGGTAaggtaggt  <  1:421566/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                      ATCACTTTGTTTTTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAGCCCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACGAAGAATTTCGGGTAGTTGCCCCAGTAGTTTACCTGGCCTTCCAGCAGCGGTTTTGGCGTGTTGGCGGTAAGGTAGGT  >  NC_000913/4084114‑4084385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑