Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,455,581	Δ1 bp	coding (29/999 nt)	fbp ←	fructose‑1,6‑bisphosphatase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,455,578	0	T	.	100.0%	236.9 / NA	56	coding (32/999 nt)	fbp	fructose‑1,6‑bisphosphatase 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (29/27);  total (29/27)

GTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGCTTTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAATCTCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGA  >  NC_000913/4455442‑4455709                                                                                                                                         |                                                                                                                                    gTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGCtttc                                                                                                                                  <  1:809077/140‑2 (MQ=255)    ctcAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGCtttcgac                                                                                                                               <  1:54321/140‑5 (MQ=255)      cAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACaa                                                                                                                            <  2:363820/140‑1 (MQ=255)              cTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGCtttc                                                                                                                                  <  1:332725/127‑2 (MQ=255)              cTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGCtttc                                                                                                                                  >  2:332725/1‑126 (MQ=255)              cTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATaaa                                                                                                                        <  1:514885/136‑1 (MQ=255)              cTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATaaa                                                                                                                        >  2:514885/1‑136 (MQ=255)                      cAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCt                                                                                                                 <  1:860293/135‑1 (MQ=255)                      cAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCt                                                                                                                 >  2:860293/1‑135 (MQ=255)                      cAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGt                                                                                                            <  2:807318/140‑1 (MQ=255)                                   aGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACtt                                                                                               >  1:751/1‑140 (MQ=255)                                   aGTCCTGCTTTGTTGATATCGAGATGGATAATCTTGACGCCAAGTTTTATTGCCGACAGCAAAGGAGTGAGCTCACCGGAAACAACAAAAACCTCGCGATGC‑TTTAGACAATAAATTCACCTAAAGTTTTCATAAAACtt                                                                                               >  2:700921/1‑140 (MQ=255)                                     tCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTc                                                                                             >  2:8727/1‑140 (MQ=255)                                          cTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGAAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGc                                                                                        >  1:57612/1‑140 (MQ=255)                                               ttgATATCGCGGTGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAGGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTcc                                                                                            >  2:736925/1‑131 (MQ=255)                                               ttgATATCGCGGTGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAGGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTcc                                                                                            <  1:736925/131‑1 (MQ=255)                                               ttgATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTcc                                                                                            >  1:959329/1‑131 (MQ=255)                                               ttgATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTcc                                                                                            <  2:959329/131‑1 (MQ=255)                                               ttgATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGt                                                                                   <  2:829576/140‑1 (MQ=255)                                               ttgATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGt                                                                                   >  2:307897/1‑140 (MQ=255)                                               ttgATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGt                                                                                   >  2:260617/1‑140 (MQ=255)                                                 gATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGttt                                                                                 <  1:835871/140‑1 (MQ=255)                                                  atatCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTa                                                                                <  1:307897/140‑1 (MQ=255)                                                    atCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCaa                                                                                      >  2:416117/1‑132 (MQ=255)                                                    atCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCaa                                                                                      <  1:416117/132‑1 (MQ=255)                                                     tCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTa                                                                                >  1:175654/1‑137 (MQ=255)                                                     tCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTa                                                                                <  2:175654/137‑1 (MQ=255)                                                      cgcgATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGc                                                                                        <  2:239526/128‑1 (MQ=255)                                                      cgcgATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGc                                                                                        >  1:239526/1‑128 (MQ=255)                                                      cgcgATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGa                                                                            >  1:567073/1‑140 (MQ=255)                                                         gATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTa                                                                         >  2:675262/1‑140 (MQ=255)                                                         gATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTa                                                                         >  2:389756/1‑140 (MQ=255)                                                            ggATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAg                                                                      >  1:274839/1‑140 (MQ=255)                                                               tAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGa                                                                   <  2:325691/140‑1 (MQ=255)                                                                        cgcCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGcaacaa                                                          >  2:515223/1‑140 (MQ=255)                                                                          cccAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATc                                                        >  1:520691/1‑140 (MQ=255)                                                                                   aTTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAaat                                               <  1:818583/140‑1 (MQ=255)                                                                                        cGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAAtct                                          <  2:274839/140‑1 (MQ=255)                                                                                         gACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACa                                                  >  2:542655/1‑131 (MQ=255)                                                                                         gACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACa                                                  <  1:542655/131‑1 (MQ=255)                                                                                             gCAAAGCAGTGAGCTCACCGGTAGCACGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAATCTCAATg                                     <  1:675262/140‑1 (MQ=255)                                                                                                    aGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAATCTCAATGTTGCGCt                              <  1:8727/140‑1 (MQ=255)                                                                                                      tGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAATCTCAATGTTGCGCTCa                            >  1:776899/1‑140 (MQ=255)                                                                                                             aCCGGTAGCATGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAATCTCAATGTTGCGCTCAGGTGAAt                     >  2:491509/1‑140 (MQ=255)                                                                                                                       tGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAATCTCAATGTTGCGCTCAGGTGAATCGCGCCAGCa           >  1:516723/1‑140 (MQ=255)                                                                                                                       tGAGAAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAATCTCAATGTTGCGCTCAGGTGAATCGCGCCAGCa           >  2:372011/1‑140 (MQ=255)                                                                                                                         agaAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAATCTCAATGTTGCGCTCAGGTGAATCGCGCCAGCaaa         >  2:951623/1‑140 (MQ=255)                                                                                                                         agaAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAATCTCAATGTTGCGCTCAGGTGAATCGCGCCAGCaaa         >  1:363090/1‑140 (MQ=255)                                                                                                                         agaAAACTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAATCTCAATGTTGCGCTCAGGTGAATCGCGCCAGCaaa         >  2:591116/1‑140 (MQ=255)                                                                                                                           aaaaCTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAATCTCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAAtt       <  1:951623/140‑1 (MQ=255)                                                                                                                              aCTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAATCTCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACg    <  2:634947/140‑1 (MQ=255)                                                                                                                               cTCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAATCTCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACgg   <  2:76561/140‑1 (MQ=255)                                                                                                                                tCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAATCTCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGa  <  1:205492/140‑1 (MQ=255)                                                                                                                                tCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAATCTCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGa  <  2:776899/140‑1 (MQ=255)                                                                                                                                tCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAATCTCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGa  <  2:567073/140‑1 (MQ=255)                                                                                                                                tCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAATCTCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGa  <  2:363090/140‑1 (MQ=255)                                                                                                                                tCGTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAATCTCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGa  >  2:205492/1‑140 (MQ=255)                                                                                                                                  gTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAATCTCAATGTTGCGCTCAGGTGAATCgcgc                <  1:376941/124‑1 (MQ=255)                                                                                                                                  gTGCTGC‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAATCTCAATGTTGCGCTCAGGTGAATCgcgc                >  2:376941/1‑124 (MQ=255)                                                                                                                                   tgctgc‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAATCTCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGa  <  1:449948/137‑1 (MQ=255)                                                                                                                                   tgctgc‑TTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAATCTCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGa  >  2:449948/1‑137 (MQ=255)                                                                                                                                         |                                                                                                                                    GTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAAAACTCGTGCTGCTTTTCGACAATAAATTCACCTAACGTTTTCATAAAACTTTCCCTGCAATGTTTATGGAGTAAAGCGACCGCAACAATCTTAACAAATAATCTCAATGTTGCGCTCAGGTGAATCGCGCCAGCAAATTACGGA  >  NC_000913/4455442‑4455709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑