Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	527,915	(A)5→4	coding (335/369 nt)	ybbC →	PF15631 family protein YbbC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	527,911	0	A	.	100.0%	268.3 / NA	63	coding (331/369 nt)	ybbC	PF15631 family protein YbbC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (31/32);  total (31/32)

TAGGTATCGCTATGGCGAACGGATTGCAGAAGAAGAAAAACCATATTTAATTACGGAACTACCAGATAGTTGGGTTGTTGAGGGAGCAAAGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAGAAAAATGGATGTGTTTTGAATTTCCTACATAGTAAATAATGCTGGCGCTGATGGATGCGGATGGAAACATTGCGTGGAGCGGGGAGTATGATGAGTGGGGCAACCAGCTGAATGAAGAGAACCCGCATCACCTG  >  NC_000913/527772‑528044                                                                                                                                            |                                                                                                                                      tAGGTATCGCTATGGCGAACGGATTGCAGAAGAAGAAAAACCATATTTAATTACGGAACTACCAGATAGTTGGGTTGTTGAGGGAGCAAAGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAGa                                                                                                                                       <  1:808381/140‑1 (MQ=255) tAGGTATCGCTATGGCGAACGGATTGCAGAAGAAGAAAAACCATATTTAATTACGGAACTACCAGATAGTTGGGTTGTTGAGGGAGCAAAGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAGa                                                                                                                                       >  2:808381/1‑140 (MQ=255)      aTCGCTATGGCGAACGGATTGCAGAAGAAGAAAAACCATATTTAATTACGGAACTACCAGATAGTTGGGTTGTTGAGGGAGCAAAGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAGaaaatg                                                                                                                                  >  2:908108/1‑138 (MQ=255)        cGCTATGGCGAACGGATTGCAGAAGAAGAAAAACCATATTTAATTACGGAACTACCAGATAGTTGGGTTGTTGAGGGAGCAAAGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAGaaaatgga                                                                                                                                >  2:119990/1‑136 (MQ=255)        cGCTATGGCGAACGGATTGCAGAAGAAGAAAAACCATATTTAATTACGGAACTACCAGATAGTTGGGTTGTTGAGGGAGCAAAGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAGaaaatgga                                                                                                                                >  2:543208/1‑136 (MQ=255)        cGCTATGGCGAACGGATTGCAGAAGAAGAAAAACCATATTTAATTACGGAACTACCAGATAGTTGGGTTGTTGAGGGAGCAAAGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAGaaaatgga                                                                                                                                >  2:383912/1‑136 (MQ=255)         gCTATGGCGAACGGATTGCAGAAGAAGAAAAACCATATTTAATTACGGAACTACCAGATAGTTGGGTTGTTGAGGGAGCAAAGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAG‑AAAATGGAt                                                                                                                              <  1:860968/140‑1 (MQ=255)         gCTATGGCGAACGGATTGCAGAAGAAGAAAAACCATATTTAATTACGGAACTACCAGATAGTTGGGTTGTTGAGGGAGCAAAGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAG‑AAAATGGAt                                                                                                                              <  1:711039/140‑1 (MQ=255)              ggCGAACGGATTGCAGAAGAAGAAAAACCATATTTAATTACGGAACTACCAGATAGTTGGGTTGTTGAGGGAGCAAAGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAG‑AAAATGGATGTGtt                                                                                                                         >  2:444869/1‑140 (MQ=255)               gCGAACGGATTGCAGAAGAAGAAAAACCATATTTAATTACGGAACTACCAGATAGTTGGGTTGTTGAGGGAGCAAAGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAG‑AAAATGGATGTGttt                                                                                                                        >  1:581347/1‑140 (MQ=255)                  aaCGGATTGCAGAAGAAGAAAAACCATATTTAATTACGGAACTACCAGATAGTTGGGTTGTTGAGGGAGCAAAGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAG‑AAAATGGAt                                                                                                                              >  2:940407/1‑131 (MQ=255)                  aaCGGATTGCAGAAGAAGAAAAACCATATTTAATTACGGAACTACCAGATAGTTGGGTTGTTGAGGGAGCAAAGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAG‑AAAATGGAt                                                                                                                              <  1:940407/131‑1 (MQ=255)                  aaCGGATTGCAGAAGAAGAAAAACCATATTTAATTACGGAACTACCAGATAGTTGGGTTGTTGAGGGAGCAAAGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAG‑AAAATGGATGTGTTTTGa                                                                                                                     <  2:415849/140‑1 (MQ=255)                  aaCGGATTGCAGAAGAAGAAAAACCATATTTAATTACGGAACTACCAGATAGTTGGGTTGTTGAGGGAGCAAAGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAG‑AAAATGGATGTGTTTTGa                                                                                                                     <  1:813809/140‑1 (MQ=255)                  aaCGGATTGCAGAAGAAGAAAAACCATATTTAATTACGGAACTACCAGATAGTTGGGTTGTTGAGGGAGCAAAGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAG‑AAAATGGATGTGTTTTGa                                                                                                                     >  2:213102/1‑140 (MQ=255)                    cGGATTGCAGAAGAAGAAAAACCATATTTAATTACGGAACTACCAGATAGTTGGGTTGTTGAGGGAGCAAAGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAG‑AAAATGGATGTGTTTTGAAt                                                                                                                   <  1:35457/140‑1 (MQ=255)                         tGCAGAAGAAGAAAAACCATATTTAATTACGGAACTACCAGATAGTTGGGTTGTTGAGGGAGCAAAGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCt                                                                                                              >  1:321027/1‑140 (MQ=255)                          gCAGAAGAAGAAAAACCATATTTAATTACGGAACTACCAGATAGTTGGGTTGTTGAGGGAGCAAAGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTa                                                                                                             <  2:639008/140‑1 (MQ=255)                           cAGAAGAAGAAAAACCATATTTAATTACGGAACTACCAGATAGTTGGGTTGTTGAGGGAGCAAAGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTAc                                                                                                            <  1:908108/140‑1 (MQ=255)                                 aagaaAAACCATATTTAATTACGGAACTACCAGATAGTTGGGTTGTTGAGGGAGCAAAGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACa                                                                                                           <  1:888107/135‑1 (MQ=255)                                 aagaaAAACCATATTTAATTACGGAACTACCAGATAGTTGGGTTGTTGAGGGAGCAAAGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACa                                                                                                           >  2:888107/1‑135 (MQ=255)                                 aagaaAAACCATATTTAATTACGGAACTACCAGATAGTTGGGTTGTTGAGGGAGCAAAGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACATAGTa                                                                                                      >  2:950736/1‑140 (MQ=255)                                    aaaaaCCATATTTAATTACGGAACTACCAGATAGTTGGGTTGTTGAGGGAGCAAAGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACATAGTAaat                                                                                                   <  1:950736/140‑1 (MQ=255)                                     aaaaCCATATTTAATTACGGAACTACCAGATAGTTGGGTTGTTGAAGGAGCAAAGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACATAGTAaata                                                                                                  >  1:278679/1‑140 (MQ=255)                                        aCCATATTTAATTACGGAACTACCAGATAGTTGGGTTGTTGAGGGAGCAAAGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACATAGTAAATAATg                                                                                               <  1:543208/140‑1 (MQ=255)                                         ccATATTTAATTACGGAACTACCAGATAGTTGGGTTGTTGAGGGAGCAAAGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACATAGTAAATAATGc                                                                                              <  2:821121/140‑1 (MQ=255)                                                        gAACTACCAGATAGTTGGGTTGTTGAGGGAGCAAAGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACATAGTAAATAATGCTGGCGCTgatggatg                                                                               <  1:86473/140‑1 (MQ=255)                                                           cTACCAGATAGTTGGGTTGTTGAGGGAGCAAAGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACATAGTAAATAATGCTGGCGCTGATGGATGCgg                                                                            >  1:246647/1‑140 (MQ=255)                                                                   tAGTTGGGTTGTTGAGGGAGCAAAGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAG‑AAAATGGATGTGTTTTGAGTTTCCTACATAGTAAATAGTGCTGGCGCTGATGGATGCGGATGGAAAc                                                                    >  2:957587/1‑140 (MQ=255)                                                                      ttGGGTTGTTGAGGGAGCAAAGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACATAGTAAATAATGCTGGCGCTGATGGATGCGGATGGAAACAtt                                                                 >  1:355886/1‑140 (MQ=255)                                                                        gggTTGTTGAGGGAGCAAAGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACATAGTAAATAATGCTGGCGCTGATGGATGCGGATGGAAACATTGc                                                               <  1:164394/140‑1 (MQ=255)                                                                             gttgAGGGAGCAAAGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACATAGTAAATAATGCTGGCGCTGATGGATGCGGATGGAAACATTGCGTGGa                                                          <  1:444869/140‑1 (MQ=255)                                                                              ttgAGGGAGCAAAGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACATAGTAAATAATGCTGGCGCTGATGGATGCGGATGGAAACATTGCGTGGAg                                                         >  1:674589/1‑140 (MQ=255)                                                                               tgAGGGAGCAAAGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACATAGTAAATAATGCTGGCGCTGATGGATGCGGATGGAAACATTGCGTGGAGc                                                        >  1:147479/1‑140 (MQ=255)                                                                                gAGGGAGCAAAGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACATAGTAAATAATGCTGGCGCTGATGGATGCGGATGGAAACATTGCGTGGAGCg                                                       >  1:32973/1‑140 (MQ=255)                                                                                gAGGGAGCAAAGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACATAGTAAATAATGCTGGCGCTGATGGATGCGGATGGAAACATTGCGTGGAGCg                                                       <  2:32973/140‑1 (MQ=255)                                                                                gAGGGAGCAAAGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACATAGTAAATAATGCTGGCGCTGATGGATGCGGATGGAAACATTGCGTGGAGCg                                                       <  2:147479/140‑1 (MQ=255)                                                                                 aGGGAGCAAAGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACATAGTAAATAATGCTGGCGCTGATGGATGCGGATGGAAACATTGCGTGGAGCgg                                                      <  2:246647/140‑1 (MQ=255)                                                                                  aggAGCAAAGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACATAGTAAATAATGCTGGCGCTGATGGATGCGGATGGAAACATTGCGTGGAGCggg                                                     <  2:278679/139‑1 (MQ=255)                                                                                   ggAGCAAAGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACATAGTAAATAATGCTGGCGCTGATGGATGCGGATGGAAACATTGCGTGGAGCgggg                                                    <  1:119990/140‑1 (MQ=255)                                                                                   ggAGCAAAGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACATAGTAAATAATGCTGGCGCTGATGGATGCGGATGGAAACATTGCGTGGAGCgggg                                                    <  1:830891/140‑1 (MQ=255)                                                                                     aGCAAAGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACATAGTAAATAATGCTGGCGCTGATGGATGCGGATGGAAACATTGCGTGGAGCgggg                                                    <  1:235509/138‑1 (MQ=255)                                                                                     aGCAAAGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACATAGTAAATAATGCTGGCGCTGATGGATGCGGATGGAAACATTGCGTGGAGCgggg                                                    >  2:235509/1‑138 (MQ=255)                                                                                       cAAAGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACATAGTAAATAATGCTGGCGCTGATGGATGCGGATGGAAACATTGCGTGGAGCgg                                                      >  1:416485/1‑134 (MQ=255)                                                                                       cAAAGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACATAGTAAATAATGCTGGCGCTGATGGATGCGGATGGAAACATTGCGTGGAGCgg                                                      <  2:416485/134‑1 (MQ=255)                                                                                        aaaGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACATAGTAAATAATGCTGGCGCTGATGGATGCGGATGGAAACATTGCGTGGAGCgggg                                                    >  1:782060/1‑135 (MQ=255)                                                                                        aaaGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACATAGTAAATAATGCTGGCGCTGATGGATGCGGATGGAAACATTGCGTGGAGCgggg                                                    <  2:782060/135‑1 (MQ=255)                                                                                           gTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACATAGTAAATAATGCTGGCGCTGATGGATGCGGATGGAAACATTGCGTGGAGCGGGGAGTatgat                                            >  1:323422/1‑140 (MQ=255)                                                                                             tACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACATAGTAAATAATGCTGGCGCTGATGGATGCGGATGGAAACATTGCGTGGAGCGGGGAGTAtgatga                                          <  2:323422/140‑1 (MQ=255)                                                                                                     gAAGTTGCGGGTGGTGTATTTATTACAGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACATAGTAAATAATGCTGGCGCTGATGGATGCGGATGGAAACATTGCGTGGAGCGGGGAGTATGATGAGTGGGGCa                                  <  1:475941/140‑1 (MQ=255)                                                                                                     gAAGTTGCGGGTGGTGTATTTATTACAGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACATAGTAAATAATGCTGGCGCTGATGGATGCGGATGGAAACATTGCGTGGAGCGGGGAGTATGATGAGTGGGGCa                                  >  2:475941/1‑140 (MQ=255)                                                                                                      aaGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACATAGTAAATAATGCTGGCGCTGATGGATGCGGATGGAAACATTGCGTGGAGCGGGGAGTATGATGAGTGGGGCaa                                 <  2:674589/140‑1 (MQ=255)                                                                                                         ttGCGGGTGGTGTATTTATTATAGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACATAGTAAATAATGCTGGCGCTGATGGATGCGGATGGAAACATTGCGTGGAGCGGGGAGTATGATGAGTGGGGCAACCa                              <  1:85227/140‑1 (MQ=255)                                                                                                          tGCGGGTGGTGTATTTATTATAGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACATAGTAAATAATGCTGGCGCTGATGGATGCGGATGGAAACATTGCGTGGAGCGGGGAGTATGATGAGTGGGGCAACCAg                             <  2:581347/140‑1 (MQ=255)                                                                                                              ggtggtGTATTTATTATAGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACATAGTAAATAATGCTGGCGCTGATGGATGCGGATGGAAACATTGCGTGGAGCGGGGAGTATGATGAGTGGGGCAACCAGCtga                         >  1:183201/1‑140 (MQ=255)                                                                                                                   tgtATTTATTATAGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACATAGTAAATAATGCTGGCGCTGATGGATGCGGATGGAAACATTGCGTGGAGCGGGGAGTATGATGAGTGGGGCAACCAGCtgaatgaa                    >  1:646220/1‑140 (MQ=255)                                                                                                                          attatAGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACATAGTAAATAATGCTGGCGCTGATGGATGCGGATGGAAACATTGCGTGGAGCGGGGAGTATGATGAGTGGGGCAACCAGCTGAATGAAGAGaa               <  1:261182/138‑1 (MQ=255)                                                                                                                          attatAGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACATAGTAAATAATGCTGGCGCTGATGGATGCGGATGGAAACATTGCGTGGAGCGGGGAGTATGATGAGTGGGGCAACCAGCTGAATGAAGAGaa               >  2:261182/1‑138 (MQ=255)                                                                                                                            tataGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACATAGTAAATAATGCTGGCGCTGATGGATGCGGATGGAAACATTGCGTGGAGCGGGGAGTATGATGAGTGGGGCAACCAGCTGAATGAAGAGAACCCg           >  1:602242/1‑140 (MQ=255)                                                                                                                            tataGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACATAGTAAATAATGCTGGCGATGATGGATGCGGATGGAAACATTGCGTGGAGCGGGGAGTATGATGAGTGGGGCAACCAGATGAATGAAGAGAACCCg           >  2:857024/1‑140 (MQ=255)                                                                                                                             ataGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACATAGTAAATAATGCTGGCGCTGATGGATGCGGATGGAAACATTGCGTGGAGCGGGGAGTATGATGAGTGGGGCAACCAGCTGAATGAAGAGAACCCGc          >  2:66263/1‑140 (MQ=255)                                                                                                                             ataGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACATAGTAAATAATGCTGGCGCTGATGGATGCGGATGGAAACATTGCGTGGAGCGGGGAGTATGATGAGTGGGGCAACCAGCTGAATGAAGAGAACCCGc          <  1:226893/140‑1 (MQ=255)                                                                                                                             ataGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACATAGTAAATAATGCTGGCGCTGATGGATGCGGATGGAAACATTGCGTGGAGCGGGGAGTATGATGAGTGGGGCAACCAGCTGAATGAAGAGAACCCGc          >  2:297443/1‑140 (MQ=255)                                                                                                                             ataGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACATAGTAAATAATGCTGGCGCTGATGGATGCGGATGGAAACATTGCGTGGAGCGGGGAGTATGATGAGTGGGGCAACCAGCTGAATGAAGAGAACCCGc          >  1:160917/1‑140 (MQ=255)                                                                                                                             ataGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACATAGTAAATAATGCTGGCGCTGATGGATGCGGATGGAAACATTGCGTGGAGCGGGGAGTATGATGAGTGGGGCAACCAGCTGAATGAAGAGAACCCGc          <  2:160917/140‑1 (MQ=255)                                                                                                                               aGAAATTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACATAGTAAATAATGCTGGCGCTGATGGATGCGGATGGAAACATTGCGTGGAGCGGGGAGTATGATGAGTGGGGCAACCAGCTGAATGAAGAGAACCCGCAt        >  2:474906/1‑140 (MQ=255)                                                                                                                                 aaaTTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACATAGTAAATAATGCTGGCGCTGATGGATGCGGATGGAAACATTGCGTGGAGCGGGGAGTATGATGAGTGGGGCAACCAGCTGAATGAAGAGAACCCGCATCa      >  2:539549/1‑140 (MQ=255)                                                                                                                                 aaaTTAATAAG‑AAAATGGATGTGTTTTGAATTTCCTACATAGTAAATAATGCTGGCGCTGATGGATGCGGATGGAAACATTGCGTGGAGCGGGGAGTATGATGAGTGGGGCAACCAGCTGAATGAAGAGAACCCGCATCa      >  2:79862/1‑140 (MQ=255)                                                                                                                                     taataaG‑AAAATGGATGTGTTTTGAATTTCCTACATAGTAAATAATGCTGGCACTGATGGATGCGGATGGAAACATTGCGTGGAGCGGGGAGTATGATGAGTGGGGCAACCAGCTGAATGAAGAGAACCCGCATCACCTg  >  2:582150/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                      TAGGTATCGCTATGGCGAACGGATTGCAGAAGAAGAAAAACCATATTTAATTACGGAACTACCAGATAGTTGGGTTGTTGAGGGAGCAAAGTTACCTTATGAAGTTGCGGGTGGTGTATTTATTATAGAAATTAATAAGAAAAATGGATGTGTTTTGAATTTCCTACATAGTAAATAATGCTGGCGCTGATGGATGCGGATGGAAACATTGCGTGGAGCGGGGAGTATGATGAGTGGGGCAACCAGCTGAATGAAGAGAACCCGCATCACCTG  >  NC_000913/527772‑528044

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑