Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	868,422	(C)8→7	coding (903/1872 nt)	gsiA →	glutathione ABC transporter ATP binding subunit GsiA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	868,415	0	C	.	100.0%	170.7 / NA	41	coding (896/1872 nt)	gsiA	glutathione ABC transporter ATP binding subunit GsiA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (20/21);  total (20/21)

CGAACAGATTTTTCATGCACCGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTAACGCGGGAAGTGCATGCCGTTGAG  >  NC_000913/868278‑868542                                                                                                                                          |                                                                                                                                cGAACAGATTTTTCATGCACCGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGccc                                                                                                                               <  1:113702/140‑1 (MQ=255)   aaCAGATTTTTCATGCACCGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGccccc                                                                                                                             >  2:385890/1‑140 (MQ=255)          ttttCATGCACCGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGa                                                                                                                     <  1:806760/140‑1 (MQ=255)                  cACCGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGaaaa                                                                                                             >  1:230886/1‑140 (MQ=255)                  cACCGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGaaaa                                                                                                             >  1:38797/1‑140 (MQ=255)                    ccGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACg                                                                                                           >  2:161927/1‑140 (MQ=255)                                 acacCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCg                                                                                              <  2:124792/140‑1 (MQ=255)                                     ccGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGGTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAAcc                                                                                          <  2:739874/140‑1 (MQ=255)                                        tGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGt                                                                                       >  1:457468/1‑140 (MQ=255)                                        tGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGt                                                                                       >  2:949497/1‑140 (MQ=255)                                        tGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGt                                                                                       >  1:177993/1‑140 (MQ=255)                                        tGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑‑CCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGtt                                                                                      >  2:836653/1‑140 (MQ=255)                                              gTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACg                                                                                 <  1:385890/140‑1 (MQ=255)                                               ttAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGa                                                                                <  1:949497/140‑1 (MQ=255)                                                 aGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGt                                                                              <  2:457468/140‑1 (MQ=255)                                                 aGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGt                                                                              <  1:161927/140‑1 (MQ=255)                                                              cAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTc                                                                 <  1:956060/140‑1 (MQ=255)                                                                   tGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCAc                                                               >  2:914589/1‑137 (MQ=255)                                                                   tGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCAc                                                               <  1:914589/137‑1 (MQ=255)                                                                      tGCGATGAAAGGGTTAGATTATCCCAGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGttt                                                         >  1:937673/1‑140 (MQ=255)                                                                           tGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCtt                                                    >  2:361729/1‑140 (MQ=255)                                                                               aGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTgcg                                                <  1:14964/140‑1 (MQ=255)                                                                                    tAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCg                                           <  2:85656/140‑1 (MQ=255)                                                                                    tAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCg                                           >  1:875340/1‑140 (MQ=255)                                                                                           tCCTCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTttgtt                                    >  2:804918/1‑140 (MQ=255)                                                                                           tCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTttgtt                                    >  2:468001/1‑140 (MQ=255)                                                                                            ccccGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGGGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTttgttg                                   >  2:382280/1‑140 (MQ=255)                                                                                             cccGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGa                                  <  2:828748/140‑1 (MQ=255)                                                                                              cagACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGaa                                 <  2:937673/138‑1 (MQ=255)                                                                                                 aCGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATcg                              >  1:775946/1‑140 (MQ=255)                                                                                                 aCGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATcg                              >  1:755610/1‑140 (MQ=255)                                                                                                     ttCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTa                          >  1:99394/1‑140 (MQ=255)                                                                                                      tCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGATCCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTaa                         <  2:875340/140‑1 (MQ=255)                                                                                                            tGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTAACGCggg                   <  1:784937/140‑1 (MQ=255)                                                                                                            tGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTAACGCggg                   <  1:468001/140‑1 (MQ=255)                                                                                                                atCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGTGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTAACGCGGGAAGt               >  2:794642/1‑140 (MQ=255)                                                                                                                   gCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTAACGCGGGAAGTGCa            >  2:402559/1‑140 (MQ=255)                                                                                                                   gCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTAACGCGGGAAGTGCa            <  1:382280/140‑1 (MQ=255)                                                                                                                    cTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTAACGCGGGAAGTGCAt           <  1:361729/140‑1 (MQ=255)                                                                                                                     ttGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGTGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTAACGCGGGAAGTGCATg          <  1:794642/140‑1 (MQ=255)                                                                                                                     ttGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTAACGCGGGAAGTGCATg          <  1:804918/140‑1 (MQ=255)                                                                                                                             ccAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTAACGCGGGAAGTGCATGCCGTTgag  <  2:99394/140‑1 (MQ=255)                                                                                                                             ccAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTAACGCGGGAAGTGCATGCCGTTgag  >  1:610091/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                CGAACAGATTTTTCATGCACCGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTAACGCGGGAAGTGCATGCCGTTGAG  >  NC_000913/868278‑868542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑