Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,475,119	(T)7→6	intergenic (+38/‑25)	pdxI → / → ydbD	pyridoxine 4‑dehydrogenase/DUF2773 domain‑containing protein YdbD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,475,113	0	T	.	100.0%	271.4 / NA	64	intergenic (+32/‑31)	pdxI/ydbD	pyridoxine 4‑dehydrogenase/DUF2773 domain‑containing protein YdbD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (29/35);  total (29/35)

ATCCCAGGGACGTCTTCGGTTGCGCATTTACGGGAGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCCTTTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAGGCCAGGAATTGCGGCTGGATACGTTTACTGCCCCTGTTTATGCTGAGTCTACCAGTACAGGCGGAACTTCGATGTGTTGCAAATGCAGT  >  NC_000913/1474974‑1475241                                                                                                                                            |                                                                                                                                  aTCCCAGGGACGTCTTCGGTTGCGCATTTACGGGAGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCCt                                                                                                                                   >  1:863528/1‑140 (MQ=255)             tCTTCGGTTGCGCATTTACGGGAGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCc‑ttttttctttttt                                                                                                                      >  2:694955/1‑140 (MQ=255)             tCTTCGGTTGCGCATTTACGGGAGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCc‑ttttttctttttt                                                                                                                      >  1:909022/1‑140 (MQ=255)             tCTTCGGTTGCGCATTTACGGGAGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCc‑ttttttctttttt                                                                                                                      >  1:41594/1‑140 (MQ=255)             tCTTCGGTTGCGCATTTACGGGAGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCc‑ttttttctttttt                                                                                                                      >  1:226287/1‑140 (MQ=255)             tCTTCGGTTGCGCATTTACGGGAGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCc‑ttttttctttttt                                                                                                                      >  1:598814/1‑140 (MQ=255)             tCTTCGGTTGCGCATTTACGGGAGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCCttttttc                                                                                                                             <  2:388673/134‑2 (MQ=255)             tCTTCGGTTGCGCATTTACGGGAGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCCttttttc                                                                                                                             <  2:373520/134‑2 (MQ=255)             tCTTCGGTTGCGCATTTACGGGAGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCCttttttc                                                                                                                             >  1:388673/1‑133 (MQ=255)             tCTTCGGTTGCGCATTTACGGGAGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCCttttttc                                                                                                                             >  1:373520/1‑133 (MQ=255)              cTTCGGTTGCGCATTTACGGGAGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTa                                                                                                                     <  1:782365/140‑1 (MQ=255)              cTTCGGTTGCGCATTTACGGGAGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTa                                                                                                                     <  2:598814/140‑1 (MQ=255)              cTTCGGTTGCGCATTTACGGGAGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTa                                                                                                                     <  2:41594/140‑1 (MQ=255)              cTTCGGTTGCGCATTTACGGGAGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTa                                                                                                                     <  2:72612/140‑1 (MQ=255)                 cGGTTGCGCATTTACGGGAGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATg                                                                                                                  >  2:263018/1‑140 (MQ=255)                    ttGCGCATTTACGGGAGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCa                                                                                                               <  2:863528/140‑1 (MQ=255)                       cgcATTTACGGGAGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTa                                                                                                                     >  2:431414/1‑131 (MQ=255)                       cgcATTTACGGGAGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTa                                                                                                                     <  1:431414/131‑1 (MQ=255)                       cgcATTTACGGGAGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATgg                                                                                                            >  2:874579/1‑140 (MQ=255)                               cGGGAGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCata                                                                                                    <  2:663022/140‑1 (MQ=255)                                 ggAGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCat                                                                                                     <  1:268945/137‑1 (MQ=255)                                 ggAGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCat                                                                                                     >  2:268945/1‑137 (MQ=255)                                    gaATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑Tg                                                                                              <  1:874579/140‑1 (MQ=255)                                    gaATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑Tg                                                                                              >  1:780066/1‑140 (MQ=255)                                     aaTATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGa                                                                                             <  1:709350/140‑1 (MQ=255)                                     aaTATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGa                                                                                             <  2:909022/140‑1 (MQ=255)                                       tatGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAg                                                                                           >  1:663575/1‑140 (MQ=255)                                       tatGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAg                                                                                           >  1:134403/1‑140 (MQ=255)                                         tGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAGGc                                                                                         >  1:170461/1‑140 (MQ=255)                                            ctgctgAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAGGCCAg                                                                                      >  2:435051/1‑140 (MQ=255)                                               ctgAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAGGCCAGGaa                                                                                   >  1:783886/1‑140 (MQ=255)                                               ctgAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAGGCCAGGaa                                                                                   >  2:909932/1‑140 (MQ=255)                                                  aaaaaTTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAGGCCAGGAATTg                                                                                <  1:171128/140‑1 (MQ=255)                                                     aaTTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAGGCCAGGAATTGCgg                                                                             <  2:783886/140‑1 (MQ=255)                                                     aaTTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAGGCCAGGAATTGCgg                                                                             <  2:226287/140‑1 (MQ=255)                                                        tGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAGGCCAGGAATTGCGGCTg                                                                          <  1:459556/140‑1 (MQ=255)                                                               ttCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAGGCCAGGAATTGCGGCTGGATACGt                                                                   >  2:324337/1‑140 (MQ=255)                                                                  tGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAGGCCAGGAATTGCGGCTGGATACGTTTa                                                                <  1:435051/140‑1 (MQ=255)                                                                   gAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAGGTCAGGAATTGCGGCTGGATACGTTTAc                                                               <  2:780066/140‑1 (MQ=255)                                                                   gAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAGGCCAGGAATTGCGGCTGGATACGTTTAc                                                               <  1:263018/140‑1 (MQ=255)                                                                         gtgtTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAGGCCAGGAATTGCGGCTGGATACGTTTAc                                                               <  1:871828/134‑1 (MQ=255)                                                                         gtgtTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAGGCCAGGAATTGCGGCTGGATACGTTTAc                                                               >  2:871828/1‑134 (MQ=255)                                                                         gtgtTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAGGCCAGGAATTGCGGCTGGATACGTTTACTGcccc                                                         <  1:324337/140‑1 (MQ=255)                                                                          tgttgtCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAGGCCAGGAATTGCGGCTGGATACGTTTACTGCCCCt                                                        >  1:485199/1‑140 (MQ=255)                                                                            ttgtCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAGGCCAGGAATTGCGGCTGGATACGTTTACTGCCCCTGt                                                      <  2:601359/140‑1 (MQ=255)                                                                            ttgtCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAGGCCAGGAATTGCGGCTGGATACGTTTACTGCCCCTGt                                                      >  1:851830/1‑140 (MQ=255)                                                                            ttgtCTACGTTGGATGGTATTTCGCGAGAATAACAAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAGGCCAGGAATTGCGGCTGGATACGTTTACTGCCCCTGt                                                      <  2:696411/140‑1 (MQ=255)                                                                              gtCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAGGCCAGGAATTGCGGCTGGATACGTTTACTGCCCCTGttt                                                    >  1:172337/1‑140 (MQ=255)                                                                              gtCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAGGCCAGGAATTGCGGCTGGATACGTTTACTGCCCCTGttt                                                    >  1:230718/1‑140 (MQ=255)                                                                                 tACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAGGCCAGGAATTGCGGCTGGATACGTTTACTGCCCCTGTTTATg                                                 <  1:909932/140‑1 (MQ=255)                                                                                   cGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAGGCCAGGAATTGCGGCTGGATACGTTTACTGCCCCTGTTTATGCt                                               <  2:572251/140‑1 (MQ=255)                                                                                    gTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAGGCCAGGAATTGCGGCTGGATACGTTTACTGCCCCTGTTTATGCt                                               <  1:521220/139‑1 (MQ=255)                                                                                    gTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAGGCCAGGAATTGCGGCTGGATACGTTTACTGCCCCTGTTTATGCt                                               >  2:521220/1‑139 (MQ=255)                                                                                        gATGGTATTTCGCGAGAATAACGAATATACAAAAGTGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAGGCCAGGAATTGCGGCTGGATACGTTTACTGCCCCTGTTTATGCTGAGTc                                          >  2:581161/1‑140 (MQ=255)                                                                                         attggaTTTCGCGAGAATAACGAATATACAAAAGGGAAGGATGCATTTCTC‑TTCTTTCTTTTTTAATGGCATGGAGGGCATAAGTGTGAA‑GCTATGAACTGCGGCTGGATACGTTTACTGCTCCTGTTTATGCTGAGTCt                                         >  2:404594/6‑140 (MQ=255)                                                                                         aTGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAGGCCAGGAATTGCGGCTGGATACGTTTACTGCCCCTGTTTATGCt                                               <  2:779479/134‑1 (MQ=255)                                                                                         aTGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAGGCCAGGAATTGCGGCTGGATACGTTTACTGCCCCTGTTTATGCt                                               >  1:779479/1‑134 (MQ=255)                                                                                         aTGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAGGCCAGGAATTGCGGCTGGATACGTTTACTGCCCCTGTTTATGCt                                               >  1:266450/1‑134 (MQ=255)                                                                                         aTGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAGGCCAGGAATTGCGGCTGGATACGTTTACTGCCCCTGTTTATGCt                                               <  2:266450/134‑1 (MQ=255)                                                                                         aTGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAGGCCAGGAATTGCGGCTGGATACGTTTACTGCCCCTGTTTATGCTGAGTCt                                         >  2:423138/1‑140 (MQ=255)                                                                                            gTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAGGCCAGGAATTGCGGCTGGATACGTTTACTGCCCCTGTTTATGCTGAGTCTAcc                                      <  1:810348/140‑1 (MQ=255)                                                                                              aTTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAGGCCAGGAATTGCGGCTGGATACGTTTACTGCCCCTGTTTATGCTGAGTCTACCAg                                    >  2:411694/1‑140 (MQ=255)                                                                                                 tCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAGGCCAGGAATTGCGGCTGGATACGTTTACTGCCCCTGTTTATGCTGAGTCTACCAGTAc                                 <  2:485199/140‑1 (MQ=255)                                                                                                       gaATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAGGCCAGGAATTGCGGCTGGATACGTTTACTGCCCCTGTTTATGCTGAGTCTACCAGTACAGGCgg                           >  1:398665/1‑140 (MQ=255)                                                                                                       gaATAACGAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGGGCATATGT‑TGAAGGCCAGGAATTGCGGCTGGATACGTTTACTGCCCCTGTTTATGCTGAGTCTACCAGTACAGGCgg                           >  2:435873/1‑140 (MQ=255)                                                                                                              gAATATACAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAGGCCAGGAATTGCGGCTGGATACGTTTACTGCCCCTGTTTATGCTGAGTCTACCAGTACAGGCGGAACTTCg                    <  2:851830/140‑1 (MQ=255)                                                                                                                  ataCAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAGGCCAGGAATTGCGGCTGGATACGTTTACTGCCCCTGTTTATGCTGAGTCTACCAGTACAGGCGGAACTTCGAtgt                >  1:394627/1‑140 (MQ=255)                                                                                                                     cAAAAGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAGGCCAGGAATTGCGGCTGGATACGTTTACTGCCCCTGTTTATGCTGAGTCTACCAGTACAGGCGGAACTTCGATGTGtt             >  2:912283/1‑140 (MQ=255)                                                                                                                        aaGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAGGCCAGGAATTGCGGCTGGATACGTTTACTGCCCCTGTTTATGCTGAGTCTACCAGTACAGGCGGAACTTCGAtgt                >  1:422270/1‑134 (MQ=255)                                                                                                                        aaGGGAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAGGCCAGGAATTGCGGCTGGATACGTTTACTGCCCCTGTTTATGCTGAGTCTACCAGTACAGGCGGAACTTCGAtgt                <  2:422270/134‑1 (MQ=255)                                                                                                                           tgAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAGGCCAGGAATTGCGGCTGGATACGTTTACTGCCCCTGTTTATGCTGAGTCTACCAGTACAGGCGGAACTTCGATGTGTTGCAAAt       <  1:581161/139‑1 (MQ=255)                                                                                                                           ggAAAGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAGGCCAGGAATTGCGGCTGGATACGTTTACTGCCCCTGTTTATGCTGAGTCTACCAGTACAGGCGGAACTTCGATGTGTTGCAAAt       <  1:694955/140‑1 (MQ=255)                                                                                                                             aaaGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAGGCCAGGAATTGCGGCTGGATACGTTTACTGCCCCTGTTTATGCTGAGTCTACCAGTACAGGCGGAACTTCg                    <  2:897292/125‑1 (MQ=255)                                                                                                                             aaaGATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAGGCCAGGAATTGCGGCTGGATACGTTTACTGCCCCTGTTTATGCTGAGTCTACCAGTACAGGCGGAACTTCg                    >  1:897292/1‑125 (MQ=255)                                                                                                                                gATGCATTTCCC‑TTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAGGCCAGGAATTGCGGCTGGATACGTTTACTGCCCCTGTTTATGCTGAGTCTACCAGTACAGGCGGAACTTCGATGTGTTGCAAATGCAGt  <  2:170461/140‑1 (MQ=255)                                                                                                                                            |                                                                                                                                  ATCCCAGGGACGTCTTCGGTTGCGCATTTACGGGAGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTGGATGGTATTTCGCGAGAATAACGAATATACAAAAGGGAAAGATGCATTTCCCTTTTTTTCTTTTTTAATGGCATGGAGTGCATATGT‑TGAAGGCCAGGAATTGCGGCTGGATACGTTTACTGCCCCTGTTTATGCTGAGTCTACCAGTACAGGCGGAACTTCGATGTGTTGCAAATGCAGT  >  NC_000913/1474974‑1475241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑