Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,183,628	(T)7→8	coding (24/819 nt)	yegX ←	putative hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,183,621	1	.	T	100.0%	179.9 / NA	51	L11I (CTC→ATC)	yegX	putative hydrolase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (25/26);  total (25/26)

AAAGATTTAACCGGGCGATAACCGTAGAAGTGGATGTAGTCTGTAATCTGAACTGCTGTCGAATAGAAAAAATTCACCGTCTGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAG‑TTTTTTTCGAC‑TGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGAAGTGGTGAACCGGACCAATA  >  NC_000913/2183482‑2183752                                                                                                                                             |                                                                                                                                     aaaGATTTAACCGGGCGATAACCGTAGAAGTGGATGTAGTCTGTAATCTGAACTGCTGTCGAATAGAAAAAATTCACCGTCTGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAg                                                                                                                                       >  2:369972/1‑140 (MQ=255)           ccGGGCGATAACCGTAGAAGTGGATGTAGTCTGTAATCTGAACTGCTGTCGAATAGAAAAAATTCACCGTCTGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAG‑ttttttttcg                                                                                                                            <  1:836122/140‑4 (MQ=255)              ggCGATAACCGTAGAAGTGGATGTAGTCTGTAATCTGAACTGCTGTCGAATAGAAAAAATTCACCGTCTGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTTCGAC‑t                                                                                                                         >  2:763436/1‑140 (MQ=255)                cGATAACCGTAGAAGTGGATGTAGTCTGTAATCTGAACTGCTGTCGAATAGAAAAAATTCACCGTCTGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTTCGAC‑Tgg                                                                                                                       <  1:19280/140‑1 (MQ=255)                cGATAACCGTAGAAGTGGATGTAGTCTGTAATCTGAACTGCTGTCGAATAGAAAAAATTCACCGTCTGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTTCGAC‑Tgg                                                                                                                       <  1:901441/140‑1 (MQ=255)                cGATAACCGTAGAAGTGGATGTAGTCTGTAATCTGAACTGCTGTCGAATAGAAAAAATTCACCGTCTGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTTCGAC‑Tgg                                                                                                                       <  2:634904/140‑1 (MQ=255)                        gTAGAAGTGGATGTAGTCTGTAATCTGAACTGCTGTCGAATAGAAAAAATTCACCGTCTGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTTCGAC‑TGGTAATTCtt                                                                                                               <  1:763436/140‑1 (MQ=255)                         tAGAAGTGGATGTAGTCTGTAATCTGAACTGCTGTCGAATAGAAAAAATTCACCGTCTGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTTCGAC‑TGGTAATTCTTa                                                                                                              >  1:551380/1‑140 (MQ=255)                          aGAAGTGGATGTAGTCTGTAGTCTGAACTGCTGTCGAATAGAAAAAATTCACCGTCTGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTTCGAC‑TGGTAATTCTTAg                                                                                                             <  1:425767/140‑1 (MQ=255)                             aGTGGATGTAGTCTGTAATCTGAACTGCTGTCGAATAGAAAAAATTCACCGTCTGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAG‑ttttttttcga                                                                                                                           >  2:527131/1‑119 (MQ=255)                             aGTGGATGTAGTCTGTAATCTGAACTGCTGTCGAATAGAAAAAATTCACCGTCTGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAG‑ttttttttcga                                                                                                                           <  1:527131/123‑5 (MQ=255)                                 gATGTAGTCTGTAATCTGAACTGCTGTCGAATAGAAAAAATTCACCGTCTGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTTCGAC‑TGGTAATTCTTAGCTGCAtt                                                                                                      >  2:493751/1‑140 (MQ=255)                                   tGTAGTCTGTAATCTGAACTGCTGTCGAATAGAAAAAATTCACCGTCTGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTTCGAC‑TGGTAAtt                                                                                                                  >  1:247966/1‑126 (MQ=255)                                   tGTAGTCTGTAATCTGAACTGCTGTCGAATAGAAAAAATTCACCGTCTGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTTCGAC‑TGGTAAtt                                                                                                                  <  2:247966/126‑1 (MQ=255)                                      aGTCTGTAATCTGAACTGCTGTCGAATAGAAAAAATTCACCGTCTGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTTCGAC‑TGGTAATTCTTAGCTGCATTGGttt                                                                                                 >  2:726866/1‑140 (MQ=255)                                      aGTCTGTAATCTGAACTGCTGTCGAATAGAAAAAATTCACCGTCTGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTTCGAC‑TGGTAATTCTTAGCTGCATTGGttt                                                                                                 >  1:57795/1‑140 (MQ=255)                                       gTCTGTAATCTGAACTGCTGTCGAATAGAAAAAATTCACCGTCTGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTTCGAC‑TGGTAATTCTTAGCTGCATTGGTTTc                                                                                                >  1:938314/1‑140 (MQ=255)                                         cTGTAATCTGAACTGCTGTCGAATAGAAAAAATTCACCGTCTGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTTCGAC‑TGGTAATTCTTAGCTGCATTGGTTTCAt                                                                                              >  2:872958/1‑140 (MQ=255)                                         cTGTAATCTGAACTGCTGTCGAATAGAAAAAATTCACCGTCTGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTTCGAC‑TGGTAATTCTTAGCTGCATTGGTTTCAt                                                                                              >  1:866439/1‑140 (MQ=255)                                          tGTAATCTGAACTGCTGTCGAATAGAAAAAATTCACCGTCTGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTTCGAC‑TGGTAATTCTTAGCTGCATTGGTTTc                                                                                                >  2:857709/1‑137 (MQ=255)                                          tGTAATCTGAACTGCTGTCGAATAGAAAAAATTCACCGTCTGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTTCGAC‑TGGTAATTCTTAGCTGCATTGGTTTc                                                                                                <  1:857709/137‑1 (MQ=255)                                           gTAATCTGAACTGCTGTCGAATAGAAAAAATTCACCGTCTGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTTCGAC‑TGGTAATTCTTAGCTGCATTGGTTTCATcc                                                                                            <  2:866439/140‑1 (MQ=255)                                                 tGAACTGCTGTCGAATAGAAAAAATTCACCGTCTGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTTCGAC‑TGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAAt                                                                                      >  2:115107/1‑140 (MQ=255)                                                 tGAACTGCTGTCGAATAGAAAAAATTCACCGTCTGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTTCGAC‑TGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAAt                                                                                      <  2:551380/140‑1 (MQ=255)                                                 tGAACTGCTGTCGAATAGAAAAAATTCACCGTCTGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTTCGAC‑TGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAAt                                                                                      <  2:938314/140‑1 (MQ=255)                                                  gAACTGCTGTCGAATAGAAAAAATTCACCGTCTGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTTCGAC‑TGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATg                                                                                     >  2:328091/1‑140 (MQ=255)                                                     ctgctgTCGAATAGAAAAAATTCACCGTCTGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTTCGAC‑TGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCa                                                                                  <  1:248086/140‑1 (MQ=255)                                                        ctgTCGAATAGAAAAAATTCACCGTCTGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTTCGAC‑TGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTg                                                                               >  1:277627/1‑140 (MQ=255)                                                        ctgTCGAATAGAAAAAATTCACCGTCTGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTTCGAC‑TGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTg                                                                               >  1:672075/1‑140 (MQ=255)                                                        ctgTCGAATAGAAAAAATTCACCGTCTGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTTCGAC‑TGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTg                                                                               >  2:292309/1‑140 (MQ=255)                                                         tgTCGAATAGAAAAAATTCACCGTCTGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTTCGAC‑TGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGc                                                                              >  1:655539/1‑140 (MQ=255)                                                         tgTCGAATAGAAAAAATTCACCGTCTGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTTCGAC‑TGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGc                                                                              >  1:577510/1‑140 (MQ=255)                                                         tgTCGAATAGAAAAAATTCACCGTCTGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTTCGAC‑TGGTAATTCTTAGCTGCACTGGTTTCATCCCTGAATGTCAGTGc                                                                              >  2:396781/1‑140 (MQ=255)                                                                tAGAAAAAATTCACCGTCTGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTTCGAC‑TGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGc                                                                       <  1:493751/140‑1 (MQ=255)                                                                tAGAAAAAATTCACCGTCTGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTTCGAC‑TGGTAATTCTTAGCTGCACTGGTTTCATCCCTGAATGTCAGTGCCAAAGGc                                                                       <  1:396781/140‑1 (MQ=255)                                                                             ccGTCTGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTTCGAC‑TGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCaa                                                          >  2:65209/1‑140 (MQ=255)                                                                               gTCTGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTTCGAC‑TGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAag                                                        <  2:672075/140‑1 (MQ=255)                                                                                  tGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTTCGAC‑TGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAagcag                                                     <  2:57795/140‑1 (MQ=255)                                                                                         gATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTTCGAC‑TGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAAc                                                             <  2:463430/125‑1 (MQ=255)                                                                                         gATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTTCGAC‑TGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAAc                                                             >  1:463430/1‑125 (MQ=255)                                                                                          aTAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTTCGAC‑TGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTa                                             <  1:369972/140‑1 (MQ=255)                                                                                                   aaCGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTTCGAC‑TGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTca                                    >  2:336032/1‑140 (MQ=255)                                                                                                       aTGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTTCGAC‑TGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTcaccac                                <  2:655539/140‑1 (MQ=255)                                                                                                       aTGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTTCGAC‑TGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTcaccac                                <  2:577510/140‑1 (MQ=255)                                                                                                        tGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTTTCACTTGGAAATTCTTAGGGGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGCCTGACAATAACCAAAGCAGCAATAGAACGGTGCTaaccct                                >  2:575845/1‑138 (MQ=255)                                                                                                           aaaTTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTTCGAC‑TGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAgg                            <  1:115107/140‑1 (MQ=255)                                                                                                                  cccAAGAGCGCATAATAGAGAGGTGAGTTTTTTTTCGAC‑TGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTcaccac                                >  2:802604/1‑129 (MQ=255)                                                                                                                  cccAAGAGCGCATAATAGAGAGGTGAGTTTTTTTTCGAC‑TGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTcaccac                                <  1:802604/129‑1 (MQ=255)                                                                                                                          cgcATAATAGAGAGGTGAGTTTTTTTTCGAC‑TGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGAAGTGGTGaa             >  2:211788/1‑140 (MQ=255)                                                                                                                           gcATAATAGAGAGGTGAGTTTTTTTTCGAC‑TGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGAAGTGGTGAAc            <  1:65209/140‑1 (MQ=255)                                                                                                                           gcATAATAGAGAGGTGAGTTTTTTTTCGAC‑TGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGAAGTGGTGAAc            <  1:726866/140‑1 (MQ=255)                                                                                                                             ataataGAGAGGTGAGTTTTTTTTCGAC‑TGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGAAGTGGTGAACCg          <  1:68996/140‑1 (MQ=255)                                                                                                                             ataataGAGAGGTGAGTTTTTTTTCGAC‑TGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGAAGTGGTGAACCg          <  1:868263/140‑1 (MQ=255)                                                                                                                                  agagagGTGAGTTTTTTTTCGAC‑TGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGAAGTGGTGAACCGGACCa     >  1:159208/1‑140 (MQ=255)                                                                                                                                     gagGTGAGTTTTTTTTCGAC‑TGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGAAGTGGTGAACCGGACCAATa  <  1:336032/140‑1 (MQ=255)                                                                                                                                             |                                                                                                                                     AAAGATTTAACCGGGCGATAACCGTAGAAGTGGATGTAGTCTGTAATCTGAACTGCTGTCGAATAGAAAAAATTCACCGTCTGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAG‑TTTTTTTCGAC‑TGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGAAGTGGTGAACCGGACCAATA  >  NC_000913/2183482‑2183752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑