Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,606,330	(G)8→9	noncoding (72/87 nt)	leuQ ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,606,322	1	.	G	95.8%	150.7 / 0.1	48	noncoding (80/87 nt)	leuQ	tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base G (26/20);  total (27/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.92e-01

TTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGTTTT  >  NC_000913/4606183‑4606456                                                                                                                                              |                                                                                                                                       tttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGa                                                                                                                                         <  1:134371/140‑1 (MQ=255)  ttGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGa                                                                                                                                         <  2:660855/140‑1 (MQ=255)    gATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑gg                                                                                                                                      <  1:528193/140‑1 (MQ=255)      tatCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑gggg                                                                                                                                    >  1:693906/1‑140 (MQ=255)         cGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGAGCGAGCAATATCACCGTGGTTTTTGGTGCGA‑ggggggg                                                                                                                                 >  2:132429/1‑140 (MQ=255)             tCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAAAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑gggggggggac                                                                                                                             <  2:725218/140‑4 (MQ=255)                        gagggggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGGACTTGAACCCCCa                                                                                                                   <  1:910793/137‑1 (MQ=255)                        gagggggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGGACTTGAACCCCCa                                                                                                                   <  1:583421/137‑1 (MQ=255)                         agggggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑ggggg                                                                                                                                   <  1:300534/120‑1 (MQ=255)                         agggggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑ggggg                                                                                                                                   >  2:300534/3‑122 (MQ=255)                              gggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGGACTTGAACCCCCa                                                                                                                   >  2:867563/1‑134 (MQ=255)                              gggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGGACTTGAACCCCCa                                                                                                                   <  1:867563/134‑1 (MQ=255)                                  gACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAg                                                                                                         <  1:182997/140‑1 (MQ=255)                                         aCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTa                                                                                                  <  2:804059/140‑1 (MQ=255)                                                  tCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCt                                                                                            <  1:154503/137‑1 (MQ=255)                                                  tCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCt                                                                                            >  2:154503/1‑137 (MQ=255)                                                       aaGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg                                                                                    >  1:526687/1‑140 (MQ=255)                                                             acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTc                                                                              >  2:301852/1‑140 (MQ=255)                                                               tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTa                                                                            >  1:868846/1‑140 (MQ=255)                                                                    ccTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAAt                                                                       >  2:28970/1‑140 (MQ=255)                                                                       gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc                                                                    >  1:776882/1‑140 (MQ=255)                                                                         aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGGTCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                  >  2:310567/1‑140 (MQ=255)                                                                         aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgc                                                                                   >  2:335637/1‑123 (MQ=255)                                                                         aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgc                                                                                   <  1:335637/123‑1 (MQ=255)                                                                         aGCTAGCGCCTGCACCAATTCCGGCGCATTCGACCAGTGCGTGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                  <  2:693906/140‑1 (MQ=18)                                                                          gCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGcc                                                                 <  1:301852/140‑1 (MQ=255)                                                                           cTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa                                                                <  2:82085/140‑1 (MQ=255)                                                                             aGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                                                              >  2:413902/1‑140 (MQ=255)                                                                                   tCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt                                                            <  1:588432/136‑1 (MQ=255)                                                                                   tCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt                                                            >  2:588432/1‑136 (MQ=255)                                                                                   tCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCa                                                        <  2:526687/140‑1 (MQ=255)                                                                                    cTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCAt                                                       >  1:82085/1‑140 (MQ=255)                                                                                             ccGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAAt                                              <  1:28970/140‑1 (MQ=255)                                                                                               gCCACCTTCGCCCAGTGCGAGTAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCt                                           >  1:803052/1‑140 (MQ=255)                                                                                                ccACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCAt                                                  <  1:454922/133‑1 (MQ=255)                                                                                                ccACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCAt                                                  >  2:454922/1‑133 (MQ=255)                                                                                                ccACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCt                                           >  1:716936/1‑140 (MQ=255)                                                                                                     ttCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGCCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTaaaa                                      >  2:184989/1‑140 (MQ=255)                                                                                                      tCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTaaaaa                                     >  1:95315/1‑140 (MQ=255)                                                                                                        gCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGa                                   >  1:834056/1‑140 (MQ=255)                                                                                                         cccAGTGCGAGTAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAAt                                 <  2:803052/140‑1 (MQ=255)                                                                                                          ccAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAAt                                 <  2:868846/140‑1 (MQ=255)                                                                                                              tGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCt                             >  1:231453/1‑140 (MQ=255)                                                                                                              tGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCt                             >  2:871061/1‑140 (MQ=255)                                                                                                                  aGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCa                         >  2:507875/1‑140 (MQ=255)                                                                                                                  aGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCa                         >  2:39567/1‑140 (MQ=255)                                                                                                                    cAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTaaaa                                      <  1:26476/125‑1 (MQ=255)                                                                                                                    cAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTaaaa                                      >  2:26476/1‑125 (MQ=255)                                                                                                                    cAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACg                       <  2:776882/140‑1 (MQ=255)                                                                                                                       tatCAACGTGGTTTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAg                    <  1:310567/140‑1 (MQ=255)                                                                                                                             cGTGGTTTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcg                 <  2:884003/137‑1 (MQ=255)                                                                                                                             cGTGGTTTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcg                 >  1:884003/1‑137 (MQ=255)                                                                                                                                 gTTTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTa          >  2:625308/1‑140 (MQ=255)                                                                                                                                  tttttGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAg         <  1:507875/140‑1 (MQ=255)                                                                                                                                    tttGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTg       >  1:581656/1‑140 (MQ=255)                                                                                                                                         tGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGtttt  >  2:680742/1‑140 (MQ=255)                                                                                                                                              |                                                                                                                                       TTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGTTTT  >  NC_000913/4606183‑4606456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑